Reviewed, UniProtKB/Swiss-Prot P46067 (LON_ERWAM)

Names and origin

Protein names

Recommended name:
    ATP-dependent protease La
    EC=3.4.21.53

Gene names

Name:

lon

Organism

Erwinia amylovora (Fire blight bacteria)

Taxonomic identifier

552 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Erwinia

Protein attributes

Sequence length	784 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Degrades short-lived regulatory and abnormal proteins in presence of ATP. Degrades the regulatory proteins rcsA and sulA. Hydrolyzes two ATPs for each peptide bond cleaved in the protein substrate By similarity.
Catalytic activity	Hydrolysis of proteins in presence of ATP.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the peptidase S16 family. Contains 1 Lon domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Protease Serine protease
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent peptidase activity Inferred from electronic annotation. Source: InterPro serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 784	784	ATP-dependent protease La		PRO_0000076135
Regions
	Domain	10 – 202	193	Lon
	Nucleotide binding	356 – 363	8	ATP Potential
Sites
	Active site	679	1	By similarity
	Active site	722	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P46067-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 1874DAE354EB05DB	FASTA	784	87,670
	10 20 30 40 50 60 MNPERSERIE IPVLPLRDVV VYPHMVIPLF VGREKSIRCL EAAMDHDKKI MLVAQKEAST 70 80 90 100 110 120 DEPGINDLFS VGTVASILQM LKLPDATVKV LVEGLQRARI SALSDNGDHF TAKAEYLTSP 130 140 150 160 170 180 EIEEREQEVL VRTAINQFEG YIKLNKKIPP EVLTSLNSID DAARLADTVA AHMPLKLSDK 190 200 210 220 230 240 QSVLEMSDVD ERLEYLMAMM ESEIDLLQVE KRIRNRVKKQ MEKSQREYYL NEQMKAIQKE 250 260 270 280 290 300 LGEMDDAPDE NEALKRKIDA ARMPKEAREK TEAELQKLKM MSPMSAEATV VRGYIDWMVQ 310 320 330 340 350 360 VPWNARSKVE KDLQKRRQTL DTDHFGLERV KDRILEYLAV QSRVSKIKGP ILCLVGPPGV 370 380 390 400 410 420 GKTSLGQSIA KATGRKYVRM ALGGVRDEAE IRGHRRTYIG SMPGKLIQKM AKVGVKNPLF 430 440 450 460 470 480 LLDEIDKMSS DMRGDPASAL LEVLDPEQNI AFNDHYLEVD YDLSDVMFVA TSNSMNIPAP 490 500 510 520 530 540 LLDRMEVIRL SGYTEDEKLN IAKKHLLSKQ IERNALKEHE LIVDDSAIVG IIRYYTRERG 550 560 570 580 590 600 VRSLERELSK LCRKAVKTLL MDKSVKHIEI NADNLKDYLG VQRYDYGRAD SENRVGQVTG 610 620 630 640 650 660 LAWTEVGGDL LTIETACVPG KGKLTYTGSL GEVMQESIQA ALTVVRARAE KLGINGDFYE 670 680 690 700 710 720 KRDIHVHVPE GATPKDGPSA GIAMCTALVS CLTGNPVRAD VAMTGEITLR GQVLPIGGLK 730 740 750 760 770 780 EKLLAAHRGG IKTVLIPDEN KRDLEEIPEN VIADLDIHPV KRIEEVLALA LQNAPYGMQV ASVK

References

[1]

"Cloning, expression, and characterization of the lon gene of Erwinia amylovora: evidence for a heat shock response." Eastgate J.A., Taylor N., Coleman M.J., Healy B., Thompson L., Roberts I.S. J. Bacteriol. 177:932-937(1995) [PubMed: 7860603] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: OT1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X77706 Genomic DNA. Translation: CAA54779.1.
PIR	S47270.
3D structure databases
SMR	P46067. Positions 8-117, 491-584, 594-784.
ModBase	Search...
Protein family/group databases
MEROPS	S16.001.
Enzyme and pathway databases
BRENDA	3.4.21.53. 81763.
Family and domain databases
InterPro	IPR003593. ATPase_AAA+_core. IPR003959. ATPase_AAA_core. IPR008269. Pept_S16_C. IPR004815. Pept_S16_lon. IPR003111. Pept_S16_N. IPR008268. Peptidase_S16_AS. IPR001984. Peptidase_S16_C. [Graphical view]
Pfam	PF00004. AAA. 1 hit. PF02190. LON. 1 hit. PF05362. Lon_C. 1 hit. [Graphical view]
PRINTS	PR00830. ENDOLAPTASE.
SMART	SM00382. AAA. 1 hit. SM00464. LON. 1 hit. [Graphical view]
TIGRFAMs	TIGR00763. lon. 1 hit.
PROSITE	PS01046. LON_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LON_ERWAM

Accession

Primary (citable) accession number: P46067

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	June 16, 2009
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)