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P46062 (SIPA1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Signal-induced proliferation-associated protein 1

Short name=Sipa-1
Alternative name(s):
GTPase-activating protein Spa-1
Gene names
Name:Sipa1
Synonyms:Spa-1, Spa1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1037 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

GTPase activator for the nuclear Ras-related regulatory protein Rap1, Rsr1 and Ran in vitro, converting it to the putatively inactive GDP-bound state. Affects cell cycle progression.

Subcellular location

Nucleus. Note: Mostly nuclear.

Tissue specificity

Preferentially expressed in both fetal and adult lymphohematopoietic tissues.

Induction

By mitogens.

Sequence similarities

Contains 1 PDZ (DHR) domain.

Contains 1 Rap-GAP domain.

Sequence caution

The sequence BAA01973.1 differs from that shown. Reason: Frameshift at several positions.

The sequence BAA13469.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Cellular componentNucleus
   DomainCoiled coil
   Molecular functionGTPase activation
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processregulation of cell cycle

Inferred from direct assay Ref.1. Source: MGI

regulation of small GTPase mediated signal transduction

Inferred from electronic annotation. Source: InterPro

   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionGTPase activator activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10371037Signal-induced proliferation-associated protein 1
PRO_0000056745

Regions

Domain316 – 534219Rap-GAP
Domain682 – 75877PDZ
Coiled coil968 – 102558 Potential

Amino acid modifications

Modified residue621Phosphothreonine By similarity
Modified residue651Phosphoserine By similarity
Modified residue8121Phosphoserine By similarity
Modified residue8341Phosphoserine By similarity

Experimental info

Sequence conflict7381T → A in BAA13469. Ref.2
Sequence conflict7631R → Q in BAA13469. Ref.2
Sequence conflict8291T → S in BAA13469. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P46062 [UniParc].

Last modified May 2, 2002. Version 2.
Checksum: 2A30592E920A9AA5

FASTA1,037112,066
        10         20         30         40         50         60 
MWAGGVGSPR RGMAPAPTDD LFARKLRQPA RPPLTPNTFE PRPARGPLLR SGSDAGEVRP 

        70         80         90        100        110        120 
PTPASPRARA HSHEDASRPA ATPTRLFTDP LALLGLPAEE PEPTFPPVLE PRWFAHYDVQ 

       130        140        150        160        170        180 
SLLFDWAPRP RGTGSHTEAN SGTLAEGQTT TSDLLLGAPG FVSELGGEGE LGLGGPISPP 

       190        200        210        220        230        240 
VPPALPNAAV SVLEEPQTRT TTYSLEHADL GAGYYRKYFY GKEHQNFFGL DEALGPVAVS 

       250        260        270        280        290        300 
LRREEKEGSG GGTLHSYRVI VRTTQLRTLR GTISEDALPP GPPSVSPRKL LEHVAPRLSP 

       310        320        330        340        350        360 
TCLRLGSASP KVPRQLLTLD EQVLSFQRKG GILYCRAGQG SEEEMYNNQE AGAAFMQFLT 

       370        380        390        400        410        420 
LLGDVVRLKG FESYRAQLDT KTDSTGTHSL YTTYQDHEIM FHVSTMLPYT PNNQQQLLRK 

       430        440        450        460        470        480 
RHIGNDIVTI VFQEPGSKPF CPTTIRSHFQ HVFLVVRAHA PCTPHTSYRV AVSRTQDTPA 

       490        500        510        520        530        540 
FGPALPEGGG PFAANADFRA FLLAKALNGE QAAGHARQFH AMATRTRQQY LQDLATNEVT 

       550        560        570        580        590        600 
TTSLDSASRF GLPSLGGRRR ATPRSPGADV QAAGALMWGV RAAPGARVAA GAETSGPDDA 

       610        620        630        640        650        660 
EVPCLLGISA ETLVLVAPRD GRVVFNCACR DVLAWTFSEH QLDLYHGRGE AITLRLDGAP 

       670        680        690        700        710        720 
GQAVGEVVAR LQLVSRGCET RELALPRDGQ GRLGFEVDAE GFITHVERFT FAETTGLRPG 

       730        740        750        760        770        780 
ARLLRVCGQT LPKLGPETAA QMLRSAPKVC VTVLPPDESG RPRRSFSELY MLSLKEPSRR 

       790        800        810        820        830        840 
GGPEPVQDET GKLVILPPTK QLLHFCLKDS SSPPGPGDLT EERTEFLRTH NSLSSGSSLS 

       850        860        870        880        890        900 
DEAPVLPNTT PDLLLVTTAN PSAPGTDRET PPSQDQSGSP SSHEDTSDSG PELRASILPR 

       910        920        930        940        950        960 
TLSLRNSISK IMSEAGSETL EDEWQSISEI ASTCNTILES LSREGQPISE SGDPKEALKC 

       970        980        990       1000       1010       1020 
DSEPEPGSLS EKVSHLESML WKLQEDLQRE KADRAALEEE VRSLRHNNQR LLAESESAAT 

      1030 
RLLLASKHLG APTTDLA 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning of a novel mitogen-inducible nuclear protein with a Ran GTPase-activating domain that affects cell cycle progression."
Hattori M., Tsukamoto N., Nur-E-Kamal M.S.A., Rubinfeld B., Iwai K., Kubota H., Maruta H., Minato N.
Mol. Cell. Biol. 15:552-560(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: BALB/c.
[2]"Mitogen-inducible SIPA1 is mapped to the conserved syntenic groups of chromosome 19 in mouse and chromosome 11q13.3 centromeric to BCL1 in human."
Wada Y., Kubota H., Maeda M., Taniwaki M., Hattori M., Imamura S., Iwai K., Minato N.
Genomics 39:66-73(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 324-1037.
Strain: DBA/2.
[3]"Human SPA-1 product selectively expressed in lymphoid tissues is a specific GTPase-activating protein for Rap1 and Rap2."
Kurachi H., Wada Y., Tsukamoto N., Maeda M., Kubota H., Hattori M., Iwai K., Minato N.
J. Biol. Chem. 272:28081-28088(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION OF PROBABLE FRAMESHIFTS.
[4]"The phagosomal proteome in interferon-gamma-activated macrophages."
Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D11374 mRNA. Translation: BAA01973.1. Frameshift.
D87849 Genomic DNA. Translation: BAA13469.1. Different initiation.
PIRI49709.
UniGeneMm.3072.

3D structure databases

ProteinModelPortalP46062.
SMRP46062. Positions 200-534, 679-753.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000079637.

PTM databases

PhosphoSiteP46062.

Proteomic databases

PaxDbP46062.
PRIDEP46062.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

MGIMGI:107576. Sipa1.

Phylogenomic databases

eggNOGNOG292441.
HOVERGENHBG061666.
InParanoidP46062.
PhylomeDBP46062.

Gene expression databases

CleanExMM_SIPA1.
GenevestigatorP46062.

Family and domain databases

Gene3D2.30.42.10. 1 hit.
InterProIPR001478. PDZ.
IPR000331. Rap_GAP_dom.
[Graphical view]
PfamPF00595. PDZ. 1 hit.
PF02145. Rap_GAP. 1 hit.
[Graphical view]
SMARTSM00228. PDZ. 1 hit.
[Graphical view]
SUPFAMSSF50156. SSF50156. 1 hit.
PROSITEPS50106. PDZ. 1 hit.
PS50085. RAPGAP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP46062.
SOURCESearch...

Entry information

Entry nameSIPA1_MOUSE
AccessionPrimary (citable) accession number: P46062
Secondary accession number(s): P70204
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: May 2, 2002
Last modified: April 16, 2014
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot