Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P46062

- SIPA1_MOUSE

UniProt

P46062 - SIPA1_MOUSE

Protein

Signal-induced proliferation-associated protein 1

Gene

Sipa1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 2 (02 May 2002)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    GTPase activator for the nuclear Ras-related regulatory protein Rap1, Rsr1 and Ran in vitro, converting it to the putatively inactive GDP-bound state. Affects cell cycle progression.

    GO - Molecular functioni

    1. GTPase activator activity Source: UniProtKB-KW

    GO - Biological processi

    1. regulation of cell cycle Source: MGI
    2. regulation of small GTPase mediated signal transduction Source: InterPro

    Keywords - Molecular functioni

    GTPase activation

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Signal-induced proliferation-associated protein 1
    Short name:
    Sipa-1
    Alternative name(s):
    GTPase-activating protein Spa-1
    Gene namesi
    Name:Sipa1
    Synonyms:Spa-1, Spa1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Unplaced

    Organism-specific databases

    MGIiMGI:107576. Sipa1.

    Subcellular locationi

    Nucleus
    Note: Mostly nuclear.

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10371037Signal-induced proliferation-associated protein 1PRO_0000056745Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei62 – 621PhosphothreonineBy similarity
    Modified residuei65 – 651PhosphoserineBy similarity
    Modified residuei812 – 8121PhosphoserineBy similarity
    Modified residuei834 – 8341PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP46062.
    PaxDbiP46062.
    PRIDEiP46062.

    PTM databases

    PhosphoSiteiP46062.

    Expressioni

    Tissue specificityi

    Preferentially expressed in both fetal and adult lymphohematopoietic tissues.

    Inductioni

    By mitogens.

    Gene expression databases

    CleanExiMM_SIPA1.
    GenevestigatoriP46062.

    Interactioni

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000079637.

    Structurei

    3D structure databases

    ProteinModelPortaliP46062.
    SMRiP46062. Positions 200-534, 682-753.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini316 – 534219Rap-GAPPROSITE-ProRule annotationAdd
    BLAST
    Domaini682 – 75877PDZPROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili968 – 102558Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Contains 1 PDZ (DHR) domain.PROSITE-ProRule annotation
    Contains 1 Rap-GAP domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG292441.
    HOVERGENiHBG061666.
    InParanoidiP46062.
    PhylomeDBiP46062.

    Family and domain databases

    Gene3Di2.30.42.10. 1 hit.
    InterProiIPR001478. PDZ.
    IPR000331. Rap_GAP_dom.
    [Graphical view]
    PfamiPF00595. PDZ. 1 hit.
    PF02145. Rap_GAP. 1 hit.
    [Graphical view]
    SMARTiSM00228. PDZ. 1 hit.
    [Graphical view]
    SUPFAMiSSF50156. SSF50156. 1 hit.
    PROSITEiPS50106. PDZ. 1 hit.
    PS50085. RAPGAP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P46062-1 [UniParc]FASTAAdd to Basket

    « Hide

    MWAGGVGSPR RGMAPAPTDD LFARKLRQPA RPPLTPNTFE PRPARGPLLR     50
    SGSDAGEVRP PTPASPRARA HSHEDASRPA ATPTRLFTDP LALLGLPAEE 100
    PEPTFPPVLE PRWFAHYDVQ SLLFDWAPRP RGTGSHTEAN SGTLAEGQTT 150
    TSDLLLGAPG FVSELGGEGE LGLGGPISPP VPPALPNAAV SVLEEPQTRT 200
    TTYSLEHADL GAGYYRKYFY GKEHQNFFGL DEALGPVAVS LRREEKEGSG 250
    GGTLHSYRVI VRTTQLRTLR GTISEDALPP GPPSVSPRKL LEHVAPRLSP 300
    TCLRLGSASP KVPRQLLTLD EQVLSFQRKG GILYCRAGQG SEEEMYNNQE 350
    AGAAFMQFLT LLGDVVRLKG FESYRAQLDT KTDSTGTHSL YTTYQDHEIM 400
    FHVSTMLPYT PNNQQQLLRK RHIGNDIVTI VFQEPGSKPF CPTTIRSHFQ 450
    HVFLVVRAHA PCTPHTSYRV AVSRTQDTPA FGPALPEGGG PFAANADFRA 500
    FLLAKALNGE QAAGHARQFH AMATRTRQQY LQDLATNEVT TTSLDSASRF 550
    GLPSLGGRRR ATPRSPGADV QAAGALMWGV RAAPGARVAA GAETSGPDDA 600
    EVPCLLGISA ETLVLVAPRD GRVVFNCACR DVLAWTFSEH QLDLYHGRGE 650
    AITLRLDGAP GQAVGEVVAR LQLVSRGCET RELALPRDGQ GRLGFEVDAE 700
    GFITHVERFT FAETTGLRPG ARLLRVCGQT LPKLGPETAA QMLRSAPKVC 750
    VTVLPPDESG RPRRSFSELY MLSLKEPSRR GGPEPVQDET GKLVILPPTK 800
    QLLHFCLKDS SSPPGPGDLT EERTEFLRTH NSLSSGSSLS DEAPVLPNTT 850
    PDLLLVTTAN PSAPGTDRET PPSQDQSGSP SSHEDTSDSG PELRASILPR 900
    TLSLRNSISK IMSEAGSETL EDEWQSISEI ASTCNTILES LSREGQPISE 950
    SGDPKEALKC DSEPEPGSLS EKVSHLESML WKLQEDLQRE KADRAALEEE 1000
    VRSLRHNNQR LLAESESAAT RLLLASKHLG APTTDLA 1037
    Length:1,037
    Mass (Da):112,066
    Last modified:May 2, 2002 - v2
    Checksum:i2A30592E920A9AA5
    GO

    Sequence cautioni

    The sequence BAA01973.1 differs from that shown. Reason: Frameshift at several positions.
    The sequence BAA13469.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti738 – 7381T → A in BAA13469. (PubMed:9027487)Curated
    Sequence conflicti763 – 7631R → Q in BAA13469. (PubMed:9027487)Curated
    Sequence conflicti829 – 8291T → S in BAA13469. (PubMed:9027487)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D11374 mRNA. Translation: BAA01973.1. Frameshift.
    D87849 Genomic DNA. Translation: BAA13469.1. Different initiation.
    PIRiI49709.
    UniGeneiMm.3072.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D11374 mRNA. Translation: BAA01973.1 . Frameshift.
    D87849 Genomic DNA. Translation: BAA13469.1 . Different initiation.
    PIRi I49709.
    UniGenei Mm.3072.

    3D structure databases

    ProteinModelPortali P46062.
    SMRi P46062. Positions 200-534, 682-753.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000079637.

    PTM databases

    PhosphoSitei P46062.

    Proteomic databases

    MaxQBi P46062.
    PaxDbi P46062.
    PRIDEi P46062.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    MGIi MGI:107576. Sipa1.

    Phylogenomic databases

    eggNOGi NOG292441.
    HOVERGENi HBG061666.
    InParanoidi P46062.
    PhylomeDBi P46062.

    Miscellaneous databases

    PROi P46062.
    SOURCEi Search...

    Gene expression databases

    CleanExi MM_SIPA1.
    Genevestigatori P46062.

    Family and domain databases

    Gene3Di 2.30.42.10. 1 hit.
    InterProi IPR001478. PDZ.
    IPR000331. Rap_GAP_dom.
    [Graphical view ]
    Pfami PF00595. PDZ. 1 hit.
    PF02145. Rap_GAP. 1 hit.
    [Graphical view ]
    SMARTi SM00228. PDZ. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50156. SSF50156. 1 hit.
    PROSITEi PS50106. PDZ. 1 hit.
    PS50085. RAPGAP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of a novel mitogen-inducible nuclear protein with a Ran GTPase-activating domain that affects cell cycle progression."
      Hattori M., Tsukamoto N., Nur-E-Kamal M.S.A., Rubinfeld B., Iwai K., Kubota H., Maruta H., Minato N.
      Mol. Cell. Biol. 15:552-560(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BALB/c.
    2. "Mitogen-inducible SIPA1 is mapped to the conserved syntenic groups of chromosome 19 in mouse and chromosome 11q13.3 centromeric to BCL1 in human."
      Wada Y., Kubota H., Maeda M., Taniwaki M., Hattori M., Imamura S., Iwai K., Minato N.
      Genomics 39:66-73(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 324-1037.
      Strain: DBA/2.
    3. "Human SPA-1 product selectively expressed in lymphoid tissues is a specific GTPase-activating protein for Rap1 and Rap2."
      Kurachi H., Wada Y., Tsukamoto N., Maeda M., Kubota H., Hattori M., Iwai K., Minato N.
      J. Biol. Chem. 272:28081-28088(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION OF PROBABLE FRAMESHIFTS.
    4. "The phagosomal proteome in interferon-gamma-activated macrophages."
      Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
      Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiSIPA1_MOUSE
    AccessioniPrimary (citable) accession number: P46062
    Secondary accession number(s): P70204
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: May 2, 2002
    Last modified: October 1, 2014
    This is version 101 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3