P46062 (SIPA1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Signal-induced proliferation-associated protein 1
Short name=Sipa-1

Alternative name(s):
GTPase-activating protein Spa-1

Gene names

Name:	Sipa1
Synonyms:	Spa-1, Spa1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1037 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	GTPase activator for the nuclear Ras-related regulatory protein Rap1, Rsr1 and Ran in vitro, converting it to the putatively inactive GDP-bound state. Affects cell cycle progression.
Subcellular location	Nucleus. Note: Mostly nuclear.
Tissue specificity	Preferentially expressed in both fetal and adult lymphohematopoietic tissues.
Induction	By mitogens.
Sequence similarities	Contains 1 PDZ (DHR) domain. Contains 1 Rap-GAP domain.
Sequence caution	The sequence BAA01973.1 differs from that shown. Reason: Frameshift at several positions. The sequence BAA13469.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Nucleus
Domain	Coiled coil
Molecular function	GTPase activation
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	positive regulation of GTPase activity Inferred from electronic annotation. Source: GOC regulation of cell cycle Inferred from direct assay Ref.1. Source: MGI regulation of small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	GTPase activator activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1037	1037	Signal-induced proliferation-associated protein 1		PRO_0000056745
Regions
	Domain	316 – 534	219	Rap-GAP
	Domain	682 – 758	77	PDZ
	Coiled coil	968 – 1025	58	Potential
Amino acid modifications
	Modified residue	62	1	Phosphothreonine By similarity
	Modified residue	65	1	Phosphoserine By similarity
	Modified residue	812	1	Phosphoserine By similarity
	Modified residue	834	1	Phosphoserine By similarity
Experimental info
	Sequence conflict	738	1	T → A in BAA13469. Ref.2
	Sequence conflict	763	1	R → Q in BAA13469. Ref.2
	Sequence conflict	829	1	T → S in BAA13469. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P46062 [UniParc]. Last modified May 2, 2002. Version 2. Checksum: 2A30592E920A9AA5	FASTA	1,037	112,066
	10 20 30 40 50 60 MWAGGVGSPR RGMAPAPTDD LFARKLRQPA RPPLTPNTFE PRPARGPLLR SGSDAGEVRP 70 80 90 100 110 120 PTPASPRARA HSHEDASRPA ATPTRLFTDP LALLGLPAEE PEPTFPPVLE PRWFAHYDVQ 130 140 150 160 170 180 SLLFDWAPRP RGTGSHTEAN SGTLAEGQTT TSDLLLGAPG FVSELGGEGE LGLGGPISPP 190 200 210 220 230 240 VPPALPNAAV SVLEEPQTRT TTYSLEHADL GAGYYRKYFY GKEHQNFFGL DEALGPVAVS 250 260 270 280 290 300 LRREEKEGSG GGTLHSYRVI VRTTQLRTLR GTISEDALPP GPPSVSPRKL LEHVAPRLSP 310 320 330 340 350 360 TCLRLGSASP KVPRQLLTLD EQVLSFQRKG GILYCRAGQG SEEEMYNNQE AGAAFMQFLT 370 380 390 400 410 420 LLGDVVRLKG FESYRAQLDT KTDSTGTHSL YTTYQDHEIM FHVSTMLPYT PNNQQQLLRK 430 440 450 460 470 480 RHIGNDIVTI VFQEPGSKPF CPTTIRSHFQ HVFLVVRAHA PCTPHTSYRV AVSRTQDTPA 490 500 510 520 530 540 FGPALPEGGG PFAANADFRA FLLAKALNGE QAAGHARQFH AMATRTRQQY LQDLATNEVT 550 560 570 580 590 600 TTSLDSASRF GLPSLGGRRR ATPRSPGADV QAAGALMWGV RAAPGARVAA GAETSGPDDA 610 620 630 640 650 660 EVPCLLGISA ETLVLVAPRD GRVVFNCACR DVLAWTFSEH QLDLYHGRGE AITLRLDGAP 670 680 690 700 710 720 GQAVGEVVAR LQLVSRGCET RELALPRDGQ GRLGFEVDAE GFITHVERFT FAETTGLRPG 730 740 750 760 770 780 ARLLRVCGQT LPKLGPETAA QMLRSAPKVC VTVLPPDESG RPRRSFSELY MLSLKEPSRR 790 800 810 820 830 840 GGPEPVQDET GKLVILPPTK QLLHFCLKDS SSPPGPGDLT EERTEFLRTH NSLSSGSSLS 850 860 870 880 890 900 DEAPVLPNTT PDLLLVTTAN PSAPGTDRET PPSQDQSGSP SSHEDTSDSG PELRASILPR 910 920 930 940 950 960 TLSLRNSISK IMSEAGSETL EDEWQSISEI ASTCNTILES LSREGQPISE SGDPKEALKC 970 980 990 1000 1010 1020 DSEPEPGSLS EKVSHLESML WKLQEDLQRE KADRAALEEE VRSLRHNNQR LLAESESAAT 1030 RLLLASKHLG APTTDLA

References

[1]	"Molecular cloning of a novel mitogen-inducible nuclear protein with a Ran GTPase-activating domain that affects cell cycle progression." Hattori M., Tsukamoto N., Nur-E-Kamal M.S.A., Rubinfeld B., Iwai K., Kubota H., Maruta H., Minato N. Mol. Cell. Biol. 15:552-560(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c.
[2]	"Mitogen-inducible SIPA1 is mapped to the conserved syntenic groups of chromosome 19 in mouse and chromosome 11q13.3 centromeric to BCL1 in human." Wada Y., Kubota H., Maeda M., Taniwaki M., Hattori M., Imamura S., Iwai K., Minato N. Genomics 39:66-73(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 324-1037. Strain: DBA/2.
[3]	"Human SPA-1 product selectively expressed in lymphoid tissues is a specific GTPase-activating protein for Rap1 and Rap2." Kurachi H., Wada Y., Tsukamoto N., Maeda M., Kubota H., Hattori M., Iwai K., Minato N. J. Biol. Chem. 272:28081-28088(1997) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION OF PROBABLE FRAMESHIFTS.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D11374 mRNA. Translation: BAA01973.1. Frameshift. D87849 Genomic DNA. Translation: BAA13469.1. Different initiation.
IPI	IPI00129472.
PIR	I49709.
UniGene	Mm.3072.
3D structure databases
ProteinModelPortal	P46062.
SMR	P46062. Positions 200-534, 680-755.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000079637.
PTM databases
PhosphoSite	P46062.
Proteomic databases
PaxDb	P46062.
PRIDE	P46062.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
MGI	MGI:107576. Sipa1.
Phylogenomic databases
eggNOG	NOG292441.
HOVERGEN	HBG061666.
InParanoid	P46062.
OrthoDB	EOG4XPQF5.
Gene expression databases
CleanEx	MM_SIPA1.
Genevestigator	P46062.
GermOnline	ENSMUSG00000056917. Mus musculus.
Family and domain databases
InterPro	IPR001478. PDZ. IPR000331. Rap_GAP_dom. [Graphical view]
Pfam	PF00595. PDZ. 1 hit. PF02145. Rap_GAP. 1 hit. [Graphical view]
SMART	SM00228. PDZ. 1 hit. [Graphical view]
SUPFAM	SSF50156. PDZ. 1 hit.
PROSITE	PS50106. PDZ. 1 hit. PS50085. RAPGAP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
SOURCE	Search...

Entry information

Entry name

SIPA1_MOUSE

Accession

Primary (citable) accession number: P46062
Secondary accession number(s): P70204

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	May 2, 2002
Last modified:	May 1, 2013
This is version 91 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program