P46059 (S15A1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Solute carrier family 15 member 1

Alternative name(s):
Intestinal H(+)/peptide cotransporter
Oligopeptide transporter, small intestine isoform
Peptide transporter 1

Gene names

Name:	SLC15A1
Synonyms:	PEPT1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	708 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Proton-coupled intake of oligopeptides of 2 to 4 amino acids with a preference for dipeptides. May constitute a major route for the absorption of protein digestion end-products.
Subcellular location	Membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the PTR2/POT transporter (TC 2.A.17) family. [View classification]

Ontologies

Keywords
Biological process	Peptide transport Protein transport Symport Transport
Cellular component	Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane Transmembrane helix
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	digestion Traceable author statement. Source: ProtInc protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to plasma membrane Traceable author statement. Source: ProtInc membrane fraction Traceable author statement. Source: ProtInc
Molecular function	oligopeptide transporter activity Inferred from electronic annotation. Source: InterPro peptide:hydrogen symporter activity Traceable author statement. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 708	708	Solute carrier family 15 member 1		PRO_0000064304
Regions
	Transmembrane	1 – 21	21	Helical; Potential
	Topological domain	22 – 53	32	Extracellular Potential
	Transmembrane	54 – 74	21	Helical; Potential
	Topological domain	75 – 82	8	Cytoplasmic Potential
	Transmembrane	83 – 103	21	Helical; Potential
	Topological domain	104 – 118	15	Extracellular Potential
	Transmembrane	119 – 139	21	Helical; Potential
	Topological domain	140 – 161	22	Cytoplasmic Potential
	Transmembrane	162 – 182	21	Helical; Potential
	Topological domain	183 – 198	16	Extracellular Potential
	Transmembrane	199 – 219	21	Helical; Potential
	Topological domain	220 – 276	57	Cytoplasmic Potential
	Transmembrane	277 – 297	21	Helical; Potential
	Topological domain	298 – 327	30	Extracellular Potential
	Transmembrane	328 – 348	21	Helical; Potential
	Topological domain	349 – 361	13	Cytoplasmic Potential
	Transmembrane	362 – 382	21	Helical; Potential
	Topological domain	383 – 584	202	Extracellular Potential
	Transmembrane	585 – 605	21	Helical; Potential
	Topological domain	606 – 619	14	Cytoplasmic Potential
	Transmembrane	620 – 640	21	Helical; Potential
	Topological domain	641 – 645	5	Extracellular Potential
	Transmembrane	646 – 666	21	Helical; Potential
	Topological domain	667 – 708	42	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	50	1	N-linked (GlcNAc...) Potential
	Glycosylation	404	1	N-linked (GlcNAc...) Potential
	Glycosylation	408	1	N-linked (GlcNAc...) Potential
	Glycosylation	439	1	N-linked (GlcNAc...) Potential
	Glycosylation	509	1	N-linked (GlcNAc...) Potential
	Glycosylation	514	1	N-linked (GlcNAc...) Potential
	Glycosylation	562	1	N-linked (GlcNAc...) Potential
Natural variations
	Natural variant	21	1	V → I. Ref.5		VAR_029321
	Natural variant	28	1	F → Y. Ref.5		VAR_029322
	Natural variant	117	1	S → N. Ref.4 Ref.5 Corresponds to variant rs2297322 [ dbSNP | Ensembl ].		VAR_022147
	Natural variant	117	1	S → R. Ref.5 Corresponds to variant rs8187821 [ dbSNP | Ensembl ].		VAR_029323
	Natural variant	122	1	V → M. Ref.5 Corresponds to variant rs8187820 [ dbSNP | Ensembl ].		VAR_020456
	Natural variant	419	1	G → A. Ref.4 Ref.5 Corresponds to variant rs4646227 [ dbSNP | Ensembl ].		VAR_020457
	Natural variant	450	1	V → I. Ref.5 Corresponds to variant rs2274828 [ dbSNP | Ensembl ].		VAR_022148
	Natural variant	451	1	T → N. Ref.5 Corresponds to variant rs8187838 [ dbSNP | Ensembl ].		VAR_020458
	Natural variant	459	1	R → C. Corresponds to variant rs2274827 [ dbSNP | Ensembl ].		VAR_022149
	Natural variant	537	1	P → S. Ref.5 Corresponds to variant rs8187830 [ dbSNP | Ensembl ].		VAR_029324

Sequences

	Sequence		Length	Mass (Da)
	P46059 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: A75475789177A907	FASTA	708	78,806
	10 20 30 40 50 60 MGMSKSHSFF GYPLSIFFIV VNEFCERFSY YGMRAILILY FTNFISWDDN LSTAIYHTFV 70 80 90 100 110 120 ALCYLTPILG ALIADSWLGK FKTIVSLSIV YTIGQAVTSV SSINDLTDHN HDGTPDSLPV 130 140 150 160 170 180 HVVLSLIGLA LIALGTGGIK PCVSAFGGDQ FEEGQEKQRN RFFSIFYLAI NAGSLLSTII 190 200 210 220 230 240 TPMLRVQQCG IHSKQACYPL AFGVPAALMA VALIVFVLGS GMYKKFKPQG NIMGKVAKCI 250 260 270 280 290 300 GFAIKNRFRH RSKAFPKREH WLDWAKEKYD ERLISQIKMV TRVMFLYIPL PMFWALFDQQ 310 320 330 340 350 360 GSRWTLQATT MSGKIGALEI QPDQMQTVNA ILIVIMVPIF DAVLYPLIAK CGFNFTSLKK 370 380 390 400 410 420 MAVGMVLASM AFVVAAIVQV EIDKTLPVFP KGNEVQIKVL NIGNNTMNIS LPGEMVTLGP 430 440 450 460 470 480 MSQTNAFMTF DVNKLTRINI SSPGSPVTAV TDDFKQGQRH TLLVWAPNHY QVVKDGLNQK 490 500 510 520 530 540 PEKGENGIRF VNTFNELITI TMSGKVYANI SSYNASTYQF FPSGIKGFTI SSTEIPPQCQ 550 560 570 580 590 600 PNFNTFYLEF GSAYTYIVQR KNDSCPEVKV FEDISANTVN MALQIPQYFL LTCGEVVFSV 610 620 630 640 650 660 TGLEFSYSQA PSNMKSVLQA GWLLTVAVGN IIVLIVAGAG QFSKQWAEYI LFAALLLVVC 670 680 690 700 VIFAIMARFY TYINPAEIEA QFDEDEKKNR LEKSNPYFMS GANSQKQM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Human intestinal H+/peptide cotransporter. Cloning, functional expression, and chromosomal localization." Liang R., Fei Y.-J., Prasad P.D., Ramamoorthy S., Han H., Yang-Feng T.L., Hediger M.A., Ganapathy V., Leibach F.H. J. Biol. Chem. 270:6456-6463(1995) [PubMed: 7896779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Intestine.
[2]	"The DNA sequence and analysis of human chromosome 13." Dunham A., Matthews L.H., Burton J., Ashurst J.L., Howe K.L., Ashcroft K.J., Beare D.M., Burford D.C., Hunt S.E., Griffiths-Jones S., Jones M.C., Keenan S.J., Oliver K., Scott C.E., Ainscough R., Almeida J.P., Ambrose K.D., Andrews D.T. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Bannerjee R., Barlow K.F., Bates K., Beasley H., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burrill W., Carder C., Carter N.P., Chapman J.C., Clamp M.E., Clark S.Y., Clarke G., Clee C.M., Clegg S.C., Cobley V., Collins J.E., Corby N., Coville G.J., Deloukas P., Dhami P., Dunham I., Dunn M., Earthrowl M.E., Ellington A.G., Faulkner L., Frankish A.G., Frankland J., French L., Garner P., Garnett J., Gilbert J.G.R., Gilson C.J., Ghori J., Grafham D.V., Gribble S.M., Griffiths C., Hall R.E., Hammond S., Harley J.L., Hart E.A., Heath P.D., Howden P.J., Huckle E.J., Hunt P.J., Hunt A.R., Johnson C., Johnson D., Kay M., Kimberley A.M., King A., Laird G.K., Langford C.J., Lawlor S., Leongamornlert D.A., Lloyd D.M., Lloyd C., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., McLaren S.J., McMurray A., Milne S., Moore M.J.F., Nickerson T., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K.M., Rice C.M., Searle S., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Steward C.A., Sycamore N., Tester J., Thomas D.W., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Wilming L., Wray P.W., Wright M.W., Young L., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Beck S., Bentley D.R., Rogers J., Ross M.T. Nature 428:522-528(2004) [PubMed: 15057823] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"Catalog of 238 variations among six human genes encoding solute carriers (hSLCs) in the Japanese population." Saito S., Iida A., Sekine A., Ogawa C., Kawauchi S., Higuchi S., Nakamura Y. J. Hum. Genet. 47:576-584(2002) [PubMed: 12436193] [Abstract] Cited for: VARIANTS ASN-117 AND ALA-419.
[5]	"Genetic variants of the human dipeptide transporter PEPT1." Anderle P., Nielsen C.U., Pinsonneault J., Krog P.L., Brodin B., Sadee W. J. Pharmacol. Exp. Ther. 316:636-646(2006) [PubMed: 16258023] [Abstract] Cited for: VARIANTS ILE-21; TYR-28; ASN-117; ARG-117; MET-122; ALA-419; ILE-450; ASN-451 AND SER-537.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U13173 mRNA. Translation: AAB61693.1. U21936 mRNA. Translation: AAA63797.1. AL391670, AL353574 Genomic DNA. Translation: CAH70393.1. AL353574, AL391670 Genomic DNA. Translation: CAI15619.1. BC096328 mRNA. Translation: AAH96328.1. BC096329 mRNA. Translation: AAH96329.1.
IPI	IPI00004279.
PIR	A56163.
RefSeq	NP_005064.1. NM_005073.3.
UniGene	Hs.436893.
3D structure databases
ProteinModelPortal	P46059.
ModBase	Search...
Protein-protein interaction databases
STRING	P46059.
PTM databases
PhosphoSite	P46059.
Polymorphism databases
DMDM	1172435.
Proteomic databases
PRIDE	P46059.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000376503; ENSP00000365686; ENSG00000088386.
GeneID	6564.
KEGG	hsa:6564.
UCSC	uc001vno.1. human.
Organism-specific databases
CTD	6564.
GeneCards	GC13M099336.
H-InvDB	HIX0037291.
HGNC	HGNC:10920. SLC15A1.
HPA	HPA002827.
MIM	600544. gene.
neXtProt	NX_P46059.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	HBG447142.
HOVERGEN	HBG052962.
InParanoid	P46059.
OMA	SEQWAEY.
OrthoDB	EOG4W6NVM.
PhylomeDB	P46059.
Enzyme and pathway databases
Reactome	REACT_15518. Transmembrane transport of small molecules. REACT_19419. Amino acid and oligopeptide SLC transporters.
Gene expression databases
ArrayExpress	P46059.
Bgee	P46059.
CleanEx	HS_SLC15A1.
Genevestigator	P46059.
GermOnline	ENSG00000088386. Homo sapiens.
Family and domain databases
InterPro	IPR016196. MFS_dom_general_subst_transpt. IPR000109. Oligopeptide_transporter. IPR004768. Pep_H_symport. IPR018456. PTR2_symporter_CS. [Graphical view]
KO	K14206.
Pfam	PF00854. PTR2. 1 hit. [Graphical view]
SUPFAM	SSF103473. MFS_gen_substrate_transporter. 1 hit.
TIGRFAMs	TIGR00926. 2A1704. 1 hit.
PROSITE	PS01022. PTR2_1. 1 hit. PS01023. PTR2_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
DrugBank	DB01140. Cefadroxil. DB01415. Ceftibuten. DB01000. Cyclacillin.
NextBio	25539.
SOURCE	Search...

Entry information

Entry name

S15A1_HUMAN

Accession

Primary (citable) accession number: P46059
Secondary accession number(s): Q5VW82

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	January 25, 2012
This is version 99 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.