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Protein

Glutamine synthetase

Gene

glnA

Organism
Frankia alni
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the ATP-dependent biosynthesis of glutamine from glutamate and ammonia.By similarity

Miscellaneous

Two forms of glutamine synthetase (GSI and GSII) can be found in this nitrogen fixing bacteria, GSI is a typical prokaryotic glutamine synthetase whereas GSII is similar to the eukaryotic enzyme.Curated

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.By similarity

Cofactori

Mg2+By similarityNote: Binds 2 Mg2+ ions per subunit.By similarity

Enzyme regulationi

The activity of this enzyme could be controlled by adenylation under conditions of abundant glutamine.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi132Magnesium 1By similarity1
Metal bindingi134Magnesium 2By similarity1
Binding sitei210ATPBy similarity1
Metal bindingi215Magnesium 2By similarity1
Metal bindingi223Magnesium 2By similarity1
Binding sitei268L-glutamate; via carbonyl oxygenBy similarity1
Metal bindingi272Magnesium 1; via pros nitrogenBy similarity1
Binding sitei276ATPBy similarity1
Binding sitei325L-glutamateBy similarity1
Binding sitei331L-glutamateBy similarity1
Binding sitei343ATPBy similarity1
Binding sitei343L-glutamateBy similarity1
Binding sitei348ATPBy similarity1
Binding sitei357ATPBy similarity1
Metal bindingi362Magnesium 1By similarity1
Binding sitei364L-glutamateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi274 – 276ATPBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processNitrogen fixation
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetaseBy similarity (EC:6.3.1.2By similarity)
Short name:
GSBy similarity
Alternative name(s):
Glutamate--ammonia ligaseBy similarity
Glutamine synthetase I betaBy similarity
Short name:
GSI betaBy similarity
Gene namesi
Name:glnABy similarity
OrganismiFrankia alni
Taxonomic identifieri1859 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaFrankialesFrankiaceaeFrankia

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001532211 – 474Glutamine synthetaseAdd BLAST474

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei402O-AMP-tyrosineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP46033.

Interactioni

Subunit structurei

Oligomer of 12 subunits arranged in the form of two hexagons.By similarity

Structurei

3D structure databases

ProteinModelPortaliP46033.
SMRiP46033.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni267 – 268L-glutamate bindingBy similarity2

Sequence similaritiesi

Belongs to the glutamine synthetase family.By similarity

Phylogenomic databases

eggNOGiENOG4105C5F. Bacteria.
COG0174. LUCA.

Family and domain databases

Gene3Di3.10.20.70. 1 hit.
3.30.590.10. 1 hit.
InterProiView protein in InterPro
IPR008147. Gln_synt_b-grasp.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR004809. Gln_synth_I.
IPR001637. Gln_synth_I_adenylation_site.
IPR027302. Gln_synth_N_conserv_site.
PfamiView protein in Pfam
PF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
SMARTiView protein in SMART
SM01230. Gln-synt_C. 1 hit.
SUPFAMiSSF54368. SSF54368. 1 hit.
TIGRFAMsiTIGR00653. GlnA. 1 hit.
PROSITEiView protein in PROSITE
PS00180. GLNA_1. 1 hit.
PS00182. GLNA_ADENYLATION. 1 hit.
PS00181. GLNA_ATP. 1 hit.

Sequencei

Sequence statusi: Complete.

P46033-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFTKAEDVLR YIRDEDVQFI DVRFCDLPGI MQHFTIPTQV FAESVFTDGL
60 70 80 90 100
MFDGSSIRGF QAIHESDMLL LPDPQTAFVD PFREHKTLAM TFFIHDPITK
110 120 130 140 150
EQYSRDPRNI AKKAETYLRG TSIADTAYFG PEAEFYIFDD VRYDYNPYGS
160 170 180 190 200
MHHVDSVEAA WNTSRKEEGG NLGYKPRFKG GYFPVPPTDH FTDLRSEMTR
210 220 230 240 250
VLYETGITVE MQHHEVGTAG QAEIDIRYDT LLKTADNLML YKYVIRNVAR
260 270 280 290 300
SRGKTVTFMP KPLFEDNGSG MHVHSSLWKD GEPLFYSPNG YGGLSDTARY
310 320 330 340 350
YIGGLLHHAP ALLAFTNPTT NSYRRLVPGY EAPVNLVYSA RNRSACCRIP
360 370 380 390 400
LGGDSPKAKR VEFRVPDPSC NPYLAFAAML MAGLDGIRNK IDPPDPIDKD
410 420 430 440 450
LYELPPDELA AVPQVPGSLE KVLDALEADN DFLREGDVFT TDLIETWLEY
460 470
KRLNEVDAIR LRPHPYEFTL YYDI
Length:474
Mass (Da):53,952
Last modified:November 1, 1995 - v1
Checksum:iFE1DB19AC1735652
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L10631 Genomic DNA. No translation available.
PIRiA40598.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiGLN1B_FRAAL
AccessioniPrimary (citable) accession number: P46033
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: July 5, 2017
This is version 82 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families