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Protein

Protein NRT1/ PTR FAMILY 8.3

Gene

NPF8.3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Peptide transporter. Mediates the transport of di- and tripeptides. High affinity, low capacity transporter. Can also transport histidine.

Enzyme regulationi

Inhibited by leucyl-ethionine.1 Publication

Kineticsi

  1. KM=14.4 µM for dileucine1 Publication

    GO - Molecular functioni

    • dipeptide transporter activity Source: TAIR
    • high-affinity oligopeptide transmembrane transporter activity Source: TAIR
    • peptide transporter activity Source: TAIR
    • tripeptide transporter activity Source: TAIR

    GO - Biological processi

    • dipeptide transport Source: TAIR
    • peptide transport Source: TAIR
    • protein transport Source: UniProtKB-KW
    • tripeptide transport Source: TAIR

    Keywordsi

    Biological processPeptide transport, Protein transport, Transport

    Enzyme and pathway databases

    ReactomeiR-ATH-427975 Proton/oligopeptide cotransporters
    R-ATH-6798695 Neutrophil degranulation

    Protein family/group databases

    TCDBi2.A.17.3.2 the proton-dependent oligopeptide transporter (pot/ptr) family

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein NRT1/ PTR FAMILY 8.3
    Short name:
    AtNPF8.3
    Alternative name(s):
    Histidine-transporting protein
    Peptide transporter PTR2
    Gene namesi
    Name:NPF8.3
    Synonyms:NTR1, PTR2, PTR2-B
    Ordered Locus Names:At2g02040
    ORF Names:F14H20.11
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 2

    Organism-specific databases

    AraportiAT2G02040
    TAIRilocus:2041125 AT2G02040

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Transmembranei91 – 111HelicalSequence analysisAdd BLAST21
    Transmembranei116 – 136HelicalSequence analysisAdd BLAST21
    Transmembranei154 – 174HelicalSequence analysisAdd BLAST21
    Transmembranei200 – 220HelicalSequence analysisAdd BLAST21
    Transmembranei228 – 248HelicalSequence analysisAdd BLAST21
    Transmembranei351 – 371HelicalSequence analysisAdd BLAST21
    Transmembranei387 – 407HelicalSequence analysisAdd BLAST21
    Transmembranei431 – 451HelicalSequence analysisAdd BLAST21
    Transmembranei472 – 492HelicalSequence analysisAdd BLAST21
    Transmembranei511 – 531HelicalSequence analysisAdd BLAST21
    Transmembranei556 – 576HelicalSequence analysisAdd BLAST21

    Keywords - Cellular componenti

    Membrane, Vacuole

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Initiator methionineiRemovedCombined sources
    ChainiPRO_00000643202 – 585Protein NRT1/ PTR FAMILY 8.3Add BLAST584

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei2N-acetylglycineCombined sources1
    Modified residuei115PhosphothreonineBy similarity1

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PaxDbiP46032

    PTM databases

    iPTMnetiP46032

    Expressioni

    Tissue specificityi

    Highly expressed in young leaves, roots and germinating seeds, intermediately in stems, flowers and mature leaves and at low level in siliques.2 Publications

    Gene expression databases

    ExpressionAtlasiP46032 baseline and differential
    GenevisibleiP46032 AT

    Interactioni

    Protein-protein interaction databases

    BioGridi138, 37 interactors
    IntActiP46032, 1 interactor
    STRINGi3702.AT2G02040.1

    Structurei

    3D structure databases

    ProteinModelPortaliP46032
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG1237 Eukaryota
    COG3104 LUCA
    HOGENOMiHOG000237400
    InParanoidiP46032
    OMAiFGADQVM
    OrthoDBiEOG093606MH
    PhylomeDBiP46032

    Family and domain databases

    CDDicd06174 MFS, 1 hit
    InterProiView protein in InterPro
    IPR020846 MFS_dom
    IPR036259 MFS_trans_sf
    IPR000109 POT_fam
    IPR018456 PTR2_symporter_CS
    PANTHERiPTHR11654 PTHR11654, 1 hit
    PfamiView protein in Pfam
    PF00854 PTR2, 1 hit
    SUPFAMiSSF103473 SSF103473, 2 hits
    PROSITEiView protein in PROSITE
    PS01022 PTR2_1, 1 hit
    PS01023 PTR2_2, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P46032-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MGSIEEEARP LIEEGLILQE VKLYAEDGSV DFNGNPPLKE KTGNWKACPF
    60 70 80 90 100
    ILGNECCERL AYYGIAGNLI TYLTTKLHQG NVSAATNVTT WQGTCYLTPL
    110 120 130 140 150
    IGAVLADAYW GRYWTIACFS GIYFIGMSAL TLSASVPALK PAECIGDFCP
    160 170 180 190 200
    SATPAQYAMF FGGLYLIALG TGGIKPCVSS FGADQFDDTD SRERVRKASF
    210 220 230 240 250
    FNWFYFSINI GALVSSSLLV WIQENRGWGL GFGIPTVFMG LAIASFFFGT
    260 270 280 290 300
    PLYRFQKPGG SPITRISQVV VASFRKSSVK VPEDATLLYE TQDKNSAIAG
    310 320 330 340 350
    SRKIEHTDDC QYLDKAAVIS EEESKSGDYS NSWRLCTVTQ VEELKILIRM
    360 370 380 390 400
    FPIWASGIIF SAVYAQMSTM FVQQGRAMNC KIGSFQLPPA ALGTFDTASV
    410 420 430 440 450
    IIWVPLYDRF IVPLARKFTG VDKGFTEIQR MGIGLFVSVL CMAAAAIVEI
    460 470 480 490 500
    IRLHMANDLG LVESGAPVPI SVLWQIPQYF ILGAAEVFYF IGQLEFFYDQ
    510 520 530 540 550
    SPDAMRSLCS ALALLTNALG NYLSSLILTL VTYFTTRNGQ EGWISDNLNS
    560 570 580
    GHLDYFFWLL AGLSLVNMAV YFFSAARYKQ KKASS
    Length:585
    Mass (Da):64,421
    Last modified:November 1, 1995 - v1
    Checksum:iC58F8194776E2D97
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti334R → ED in CAA54634 (PubMed:8033999).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    L39082 mRNA Translation: AAB00858.1
    X77503 mRNA Translation: CAA54634.1
    AC006532 Genomic DNA Translation: AAD20096.1
    CP002685 Genomic DNA Translation: AEC05538.1
    AF372946 mRNA Translation: AAK50086.1
    AY143954 mRNA Translation: AAN28893.1
    PIRiC84432
    S46236
    RefSeqiNP_178313.1, NM_126265.4
    UniGeneiAt.22261
    At.24145

    Genome annotation databases

    EnsemblPlantsiAT2G02040.1; AT2G02040.1; AT2G02040
    GeneIDi814735
    GrameneiAT2G02040.1; AT2G02040.1; AT2G02040
    KEGGiath:AT2G02040

    Similar proteinsi

    Entry informationi

    Entry nameiPTR2_ARATH
    AccessioniPrimary (citable) accession number: P46032
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: April 25, 2018
    This is version 139 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health