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Protein

Protein NRT1/ PTR FAMILY 8.3

Gene

NPF8.3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Peptide transporter. Mediates the transport of di- and tripeptides. High affinity, low capacity transporter. Can also transport histidine.

Enzyme regulationi

Inhibited by leucyl-ethionine.1 Publication

Kineticsi

  1. KM=14.4 µM for dileucine1 Publication

    GO - Molecular functioni

    • dipeptide transporter activity Source: TAIR
    • high-affinity oligopeptide transporter activity Source: TAIR
    • peptide transporter activity Source: TAIR
    • tripeptide transporter activity Source: TAIR

    GO - Biological processi

    • dipeptide transport Source: TAIR
    • peptide transport Source: TAIR
    • protein transport Source: UniProtKB-KW
    • tripeptide transport Source: TAIR
    Complete GO annotation...

    Keywords - Biological processi

    Peptide transport, Protein transport, Transport

    Protein family/group databases

    TCDBi2.A.17.3.2. the proton-dependent oligopeptide transporter (pot/ptr) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein NRT1/ PTR FAMILY 8.3
    Short name:
    AtNPF8.3
    Alternative name(s):
    Histidine-transporting protein
    Peptide transporter PTR2
    Gene namesi
    Name:NPF8.3
    Synonyms:NTR1, PTR2, PTR2-B
    Ordered Locus Names:At2g02040
    ORF Names:F14H20.11
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 2

    Organism-specific databases

    TAIRiAT2G02040.

    Subcellular locationi

    Topology

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei91 – 11121HelicalSequence analysisAdd
    BLAST
    Transmembranei116 – 13621HelicalSequence analysisAdd
    BLAST
    Transmembranei154 – 17421HelicalSequence analysisAdd
    BLAST
    Transmembranei200 – 22021HelicalSequence analysisAdd
    BLAST
    Transmembranei228 – 24821HelicalSequence analysisAdd
    BLAST
    Transmembranei351 – 37121HelicalSequence analysisAdd
    BLAST
    Transmembranei387 – 40721HelicalSequence analysisAdd
    BLAST
    Transmembranei431 – 45121HelicalSequence analysisAdd
    BLAST
    Transmembranei472 – 49221HelicalSequence analysisAdd
    BLAST
    Transmembranei511 – 53121HelicalSequence analysisAdd
    BLAST
    Transmembranei556 – 57621HelicalSequence analysisAdd
    BLAST

    GO - Cellular componenti

    • Golgi apparatus Source: TAIR
    • integral component of membrane Source: UniProtKB-KW
    • plant-type vacuole Source: TAIR
    • plant-type vacuole membrane Source: TAIR
    • vacuolar membrane Source: TAIR
    • vacuole Source: TAIR
    Complete GO annotation...

    Keywords - Cellular componenti

    Membrane, Vacuole

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methionineiRemovedCombined sources
    Chaini2 – 585584Protein NRT1/ PTR FAMILY 8.3PRO_0000064320Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylglycineCombined sources
    Modified residuei115 – 1151PhosphothreonineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PaxDbiP46032.
    PRIDEiP46032.

    Expressioni

    Tissue specificityi

    Highly expressed in young leaves, roots and germinating seeds, intermediately in stems, flowers and mature leaves and at low level in siliques.2 Publications

    Gene expression databases

    GenevisibleiP46032. AT.

    Interactioni

    Protein-protein interaction databases

    BioGridi138. 37 interactions.
    IntActiP46032. 1 interaction.
    STRINGi3702.AT2G02040.1.

    Structurei

    3D structure databases

    ProteinModelPortaliP46032.
    SMRiP46032. Positions 31-582.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG1237. Eukaryota.
    COG3104. LUCA.
    HOGENOMiHOG000237400.
    InParanoidiP46032.
    OMAiERGFTEL.
    PhylomeDBiP46032.

    Family and domain databases

    InterProiIPR020846. MFS_dom.
    IPR000109. POT_fam.
    IPR018456. PTR2_symporter_CS.
    [Graphical view]
    PfamiPF00854. PTR2. 1 hit.
    [Graphical view]
    SUPFAMiSSF103473. SSF103473. 2 hits.
    PROSITEiPS01022. PTR2_1. 1 hit.
    PS01023. PTR2_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P46032-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MGSIEEEARP LIEEGLILQE VKLYAEDGSV DFNGNPPLKE KTGNWKACPF
    60 70 80 90 100
    ILGNECCERL AYYGIAGNLI TYLTTKLHQG NVSAATNVTT WQGTCYLTPL
    110 120 130 140 150
    IGAVLADAYW GRYWTIACFS GIYFIGMSAL TLSASVPALK PAECIGDFCP
    160 170 180 190 200
    SATPAQYAMF FGGLYLIALG TGGIKPCVSS FGADQFDDTD SRERVRKASF
    210 220 230 240 250
    FNWFYFSINI GALVSSSLLV WIQENRGWGL GFGIPTVFMG LAIASFFFGT
    260 270 280 290 300
    PLYRFQKPGG SPITRISQVV VASFRKSSVK VPEDATLLYE TQDKNSAIAG
    310 320 330 340 350
    SRKIEHTDDC QYLDKAAVIS EEESKSGDYS NSWRLCTVTQ VEELKILIRM
    360 370 380 390 400
    FPIWASGIIF SAVYAQMSTM FVQQGRAMNC KIGSFQLPPA ALGTFDTASV
    410 420 430 440 450
    IIWVPLYDRF IVPLARKFTG VDKGFTEIQR MGIGLFVSVL CMAAAAIVEI
    460 470 480 490 500
    IRLHMANDLG LVESGAPVPI SVLWQIPQYF ILGAAEVFYF IGQLEFFYDQ
    510 520 530 540 550
    SPDAMRSLCS ALALLTNALG NYLSSLILTL VTYFTTRNGQ EGWISDNLNS
    560 570 580
    GHLDYFFWLL AGLSLVNMAV YFFSAARYKQ KKASS
    Length:585
    Mass (Da):64,421
    Last modified:November 1, 1995 - v1
    Checksum:iC58F8194776E2D97
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti334 – 3341R → ED in CAA54634 (PubMed:8033999).Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    L39082 mRNA. Translation: AAB00858.1.
    X77503 mRNA. Translation: CAA54634.1.
    AC006532 Genomic DNA. Translation: AAD20096.1.
    CP002685 Genomic DNA. Translation: AEC05538.1.
    AF372946 mRNA. Translation: AAK50086.1.
    AY143954 mRNA. Translation: AAN28893.1.
    PIRiC84432.
    S46236.
    RefSeqiNP_178313.1. NM_126265.3.
    UniGeneiAt.22261.
    At.24145.

    Genome annotation databases

    EnsemblPlantsiAT2G02040.1; AT2G02040.1; AT2G02040.
    GeneIDi814735.
    GrameneiAT2G02040.1; AT2G02040.1; AT2G02040.
    KEGGiath:AT2G02040.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    L39082 mRNA. Translation: AAB00858.1.
    X77503 mRNA. Translation: CAA54634.1.
    AC006532 Genomic DNA. Translation: AAD20096.1.
    CP002685 Genomic DNA. Translation: AEC05538.1.
    AF372946 mRNA. Translation: AAK50086.1.
    AY143954 mRNA. Translation: AAN28893.1.
    PIRiC84432.
    S46236.
    RefSeqiNP_178313.1. NM_126265.3.
    UniGeneiAt.22261.
    At.24145.

    3D structure databases

    ProteinModelPortaliP46032.
    SMRiP46032. Positions 31-582.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi138. 37 interactions.
    IntActiP46032. 1 interaction.
    STRINGi3702.AT2G02040.1.

    Protein family/group databases

    TCDBi2.A.17.3.2. the proton-dependent oligopeptide transporter (pot/ptr) family.

    Proteomic databases

    PaxDbiP46032.
    PRIDEiP46032.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblPlantsiAT2G02040.1; AT2G02040.1; AT2G02040.
    GeneIDi814735.
    GrameneiAT2G02040.1; AT2G02040.1; AT2G02040.
    KEGGiath:AT2G02040.

    Organism-specific databases

    TAIRiAT2G02040.

    Phylogenomic databases

    eggNOGiKOG1237. Eukaryota.
    COG3104. LUCA.
    HOGENOMiHOG000237400.
    InParanoidiP46032.
    OMAiERGFTEL.
    PhylomeDBiP46032.

    Miscellaneous databases

    PROiP46032.

    Gene expression databases

    GenevisibleiP46032. AT.

    Family and domain databases

    InterProiIPR020846. MFS_dom.
    IPR000109. POT_fam.
    IPR018456. PTR2_symporter_CS.
    [Graphical view]
    PfamiPF00854. PTR2. 1 hit.
    [Graphical view]
    SUPFAMiSSF103473. SSF103473. 2 hits.
    PROSITEiPS01022. PTR2_1. 1 hit.
    PS01023. PTR2_2. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "Cloning of a second Arabidopsis peptide transport gene."
      Song W., Steiner H.-Y., Zhang L., Naider F., Stacey G., Becker J.M.
      Plant Physiol. 110:171-178(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION, TISSUE SPECIFICITY.
      Strain: cv. Landsberg erecta.
    2. "Cloning of an Arabidopsis histidine transporting protein related to nitrate and peptide transporters."
      Frommer W.B., Hummel S., Rentsch D.
      FEBS Lett. 347:185-189(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. C24.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    4. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    6. Cited for: SUBCELLULAR LOCATION.
    7. "Nitrate transporters and peptide transporters."
      Tsay Y.F., Chiu C.C., Tsai C.B., Ho C.H., Hsu P.K.
      FEBS Lett. 581:2290-2300(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, GENE FAMILY.
    8. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
      Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
      Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT GLY-2, CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. Cited for: GENE FAMILY, NOMENCLATURE.

    Entry informationi

    Entry nameiPTR2_ARATH
    AccessioniPrimary (citable) accession number: P46032
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: February 17, 2016
    This is version 126 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.