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Protein

Biosynthetic peptidoglycan transglycosylase

Gene

mtgA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Peptidoglycan polymerase that catalyzes glycan chain elongation from lipid-linked precursors (PubMed:6368264, PubMed:8772200, PubMed:18165305). May play a role in peptidoglycan assembly during cell division in collaboration with other cell division proteins (PubMed:18165305).3 Publications

Catalytic activityi

(GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n)-diphosphoundecaprenol + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = (GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n+1)-diphosphoundecaprenol + undecaprenyl diphosphate.UniRule annotation2 Publications

Enzyme regulationi

Inhibited by EDTA (PubMed:6368264). Stimulated by Ca2+, Mn2+ and Co2+ (PubMed:6368264). Not inhibited by flavomycin (PubMed:8772200).2 Publications

pH dependencei

Optimum pH is 6.0-6.5.2 Publications

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotation1 Publication
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processCell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Enzyme and pathway databases

BioCyciEcoCyc:G7668-MONOMER.
MetaCyc:G7668-MONOMER.
UniPathwayiUPA00219.

Protein family/group databases

CAZyiGT51. Glycosyltransferase Family 51.

Names & Taxonomyi

Protein namesi
Recommended name:
Biosynthetic peptidoglycan transglycosylaseUniRule annotationCurated (EC:2.4.1.129UniRule annotation1 Publication)
Alternative name(s):
Glycan polymerase1 PublicationUniRule annotation
Monofunctional biosynthetic peptidoglycan transglycosylaseCurated
Monofunctional glycosyltransferase1 Publication
Short name:
Monofunctional GTase1 Publication
Peptidoglycan glycosyltransferase MtgAUniRule annotationCurated
Short name:
PGTUniRule annotationCurated
Gene namesi
Name:mtgA1 PublicationUniRule annotation
Synonyms:mgt1 Publication, yrbM
Ordered Locus Names:b3208, JW3175
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12808. mtgA.

Subcellular locationi

  • Cell inner membrane UniRule annotation1 Publication; Single-pass membrane protein UniRule annotation

  • Note: Localizes to the cell division site.1 Publication

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei19 – 39HelicalUniRule annotationAdd BLAST21

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • peptidoglycan-based cell wall Source: InterPro
  • plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000831251 – 242Biosynthetic peptidoglycan transglycosylaseAdd BLAST242

Proteomic databases

PaxDbiP46022.
PRIDEiP46022.

Interactioni

Subunit structurei

Interacts with FtsI, FtsW and FtsN.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
ftsIP0AD683EBI-558469,EBI-548564

Protein-protein interaction databases

BioGridi4259286. 274 interactors.
DIPiDIP-10266N.
IntActiP46022. 5 interactors.
MINTiMINT-1226871.
STRINGi316385.ECDH10B_3382.

Structurei

3D structure databases

ProteinModelPortaliP46022.
SMRiP46022.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 51 family.UniRule annotationCurated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4108VKV. Bacteria.
COG0744. LUCA.
HOGENOMiHOG000288117.
InParanoidiP46022.
KOiK03814.
PhylomeDBiP46022.

Family and domain databases

Gene3Di1.10.3810.10. 1 hit.
HAMAPiMF_00766. PGT_MtgA. 1 hit.
InterProiView protein in InterPro
IPR001264. Glyco_trans_51.
IPR023346. Lysozyme-like_dom.
IPR011812. Pep_trsgly.
PfamiView protein in Pfam
PF00912. Transgly. 1 hit.
SUPFAMiSSF53955. SSF53955. 1 hit.
TIGRFAMsiTIGR02070. mono_pep_trsgly. 1 hit.

Sequencei

Sequence statusi: Complete.

P46022-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKSRLTVFS FVRRFLLRLM VVLAVFWGGG IALFSVAPVP FSAVMVERQV
60 70 80 90 100
SAWLHGNFRY VAHSDWVSMD QISPWMGLAV IAAEDQKFPE HWGFDVASIE
110 120 130 140 150
KALAHNERNE NRIRGASTIS QQTAKNLFLW DGRSWVRKGL EAGLTLGIET
160 170 180 190 200
VWSKKRILTV YLNIAEFGDG VFGVEAAAQR YFHKPASKLT RSEAALLAAV
210 220 230 240
LPNPLRFKVS SPSGYVRSRQ AWILRQMYQL GGEPFMQQHQ LD
Length:242
Mass (Da):27,342
Last modified:November 1, 1995 - v1
Checksum:iAB3B21B91BE4BA8C
GO

Sequence cautioni

The sequence D13188 differs from that shown. Reason: Frameshift at position 38.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA. Translation: AAA58010.1.
U00096 Genomic DNA. Translation: AAC76240.1.
AP009048 Genomic DNA. Translation: BAE77252.1.
D13188 Genomic DNA. No translation available.
PIRiB65112.
RefSeqiNP_417675.1. NC_000913.3.
WP_000047091.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76240; AAC76240; b3208.
BAE77252; BAE77252; BAE77252.
GeneIDi947728.
KEGGiecj:JW3175.
eco:b3208.
PATRICifig|1411691.4.peg.3523.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiMTGA_ECOLI
AccessioniPrimary (citable) accession number: P46022
Secondary accession number(s): Q2M904
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: July 5, 2017
This is version 143 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families