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Protein

Protein phosphatase 2C 70

Gene

KAPP

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Dephosphorylates the Ser/Thr receptor-like kinase RLK5. May function as a signaling component in a pathway involving RLK5 (PubMed:15592873). Binds and dephosphorylates CLAVATA1 (CLV1). Functions as a negative regulator of the CLV1 signaling in plant development (PubMed:9294234, PubMed:9701578). Dephosphorylates SERK1 receptor kinase on threonine residues in the A-loop. Dephosphorylation of SERK1 controls SERK1 internalization (PubMed:12101128). Component of a signaling pathway which mediates adaptation to NaCl stress. Is not a component of the SALT OVERLY SENSITIVE (SOS) pathway (PubMed:18162596).5 Publications

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi346 – 3461Manganese 1By similarity
Metal bindingi346 – 3461Manganese 2By similarity
Metal bindingi347 – 3471Manganese 1; via carbonyl oxygenBy similarity
Metal bindingi521 – 5211Manganese 2By similarity
Metal bindingi568 – 5681Manganese 2By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Magnesium, Manganese, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT5G19280-MONOMER.
ARA:GQT-2080-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase 2C 70 (EC:3.1.3.16)
Short name:
AtPP2C70
Alternative name(s):
Kinase-associated protein phosphatase1 Publication
Protein ROOT ATTENUATED GROWTH 11 Publication
Gene namesi
Name:KAPP1 Publication
Synonyms:RAG11 Publication
Ordered Locus Names:At5g19280
ORF Names:F7K24.30, T24G5.3
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G19280.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 77ExtracellularSequence Analysis
Transmembranei8 – 2821HelicalSequence AnalysisAdd
BLAST
Topological domaini29 – 581553CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

No visible phenotype under normal growth conditions, but mutant seedlings show increased sensitivity of roots to salt stress.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 581581Protein phosphatase 2C 70PRO_0000057780Add
BLAST

Proteomic databases

PaxDbiP46014.
PRIDEiP46014.

Expressioni

Tissue specificityi

Expressed in all tissues examined.

Gene expression databases

ExpressionAtlasiP46014. baseline and differential.

Interactioni

Subunit structurei

Association of RLK5 with kapp domain is dependent on phosphorylation of RLK5 and can be abolished by dephosphorylation. Interacts with SERK1 and CDC48A. Component of the SERK1 signaling complex, composed of KAPP, CDC48A, GRF6 or GRF7, SERK1, SERK2, SERK3/BAK1 and BRI1 (PubMed:15592873). Interacts with CLV1 (PubMed:9294234, PubMed:9701578).3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CLV1Q9SYQ86EBI-1646157,EBI-1646111
CRK6Q9C5S94EBI-1646157,EBI-2023993
KIK1O499743EBI-1646157,EBI-2015790From a different organism.

Protein-protein interaction databases

BioGridi17324. 12 interactions.
IntActiP46014. 12 interactions.
MINTiMINT-98609.
STRINGi3702.AT5G19280.2.

Structurei

Secondary structure

1
581
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi180 – 1867Combined sources
Beta strandi195 – 1984Combined sources
Beta strandi207 – 2159Combined sources
Beta strandi217 – 2193Combined sources
Beta strandi225 – 23511Combined sources
Turni236 – 2394Combined sources
Beta strandi240 – 2456Combined sources
Beta strandi257 – 2604Combined sources
Turni264 – 2663Combined sources
Beta strandi279 – 2824Combined sources
Beta strandi284 – 2863Combined sources
Beta strandi288 – 2958Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1MZKNMR-A180-313[»]
ProteinModelPortaliP46014.
SMRiP46014. Positions 180-298, 335-577.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP46014.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini208 – 25952FHAPROSITE-ProRule annotationAdd
BLAST
Domaini304 – 577274PPM-type phosphatasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 FHA domain.PROSITE-ProRule annotation
Contains 1 PPM-type phosphatase domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0631.
HOGENOMiHOG000240507.
InParanoidiP46014.
PhylomeDBiP46014.

Family and domain databases

Gene3Di2.60.200.20. 1 hit.
3.60.40.10. 1 hit.
InterProiIPR000253. FHA_dom.
IPR016660. Kinase_assoc_Pase.
IPR015655. PP2C.
IPR000222. PP2C_BS.
IPR001932. PPM-type_phosphatase_dom.
IPR008984. SMAD_FHA_domain.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 1 hit.
PTHR13832:SF224. PTHR13832:SF224. 1 hit.
PfamiPF00498. FHA. 1 hit.
PF00481. PP2C. 1 hit.
[Graphical view]
PIRSFiPIRSF016465. Kap_phosphatase. 1 hit.
SMARTiSM00240. FHA. 1 hit.
SM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF49879. SSF49879. 1 hit.
SSF81606. SSF81606. 1 hit.
PROSITEiPS50006. FHA_DOMAIN. 1 hit.
PS01032. PPM_1. 1 hit.
PS51746. PPM_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: P46014-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAMIGMNIIG LFMVLMLLLI SLIILFACKP WRYFSRFRSS SRFSSTFKVG
60 70 80 90 100
DLQRPLISDD GNLIQGQTSE VTREYDLEGA CYQNDGLLHS SLTEGRFYKQ
110 120 130 140 150
RLPSSSPHFS QGESFVLEVI SEPSDNALVG QTLKLPAEKG SLAEVQTYDW
160 170 180 190 200
QNNRNENLQY NLEKDRLINL SPRLVEDQRS WLFLEVIAGP AIGLQHAVNS
210 220 230 240 250
TSSSKLPVKL GRVSPSDLAL KDSEVSGKHA QITWNSTKFK WELVDMGSLN
260 270 280 290 300
GTLVNSHSIS HPDLGSRKWG NPVELASDDI ITLGTTTKVY VRISSQNEFQ
310 320 330 340 350
IPFKIGVASD PMAMRRGGRK LPMEDVCHYK WPLPGANKFG LFCVCDGHGG
360 370 380 390 400
SGAAQSAIKI IPEVLANILS DSLRKEKVLS KRDASDVLRD MFAKTEARLE
410 420 430 440 450
EHQYEGCTAT VLLVWKDNEE NFFAQCANLG DSACVIHLGG RYIQMTEDHR
460 470 480 490 500
VVSLSERKRF QEAGLALRDG ETRLFGINLA RMLGDKFPKQ QDSRFSAEPY
510 520 530 540 550
ISEPLRIDQS SKDVFAVLAS DGLWDVVSPK KAVQLVLQMR DKERGRESSA
560 570 580
EKIANGLLNE ARAMRTKDNT SIIYLDFDTS L
Length:581
Mass (Da):64,911
Last modified:November 1, 1997 - v2
Checksum:iEE0C2A41E55E100D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09505 mRNA. Translation: AAB38148.1.
AC069326 Genomic DNA. No translation available.
AF296837 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED92679.1.
AY045853 mRNA. Translation: AAK76527.1.
AY117152 mRNA. Translation: AAM51227.1.
PIRiA55174.
RefSeqiNP_197429.1. NM_121933.3. [P46014-1]
UniGeneiAt.10199.

Genome annotation databases

EnsemblPlantsiAT5G19280.1; AT5G19280.1; AT5G19280. [P46014-1]
GeneIDi832048.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09505 mRNA. Translation: AAB38148.1.
AC069326 Genomic DNA. No translation available.
AF296837 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED92679.1.
AY045853 mRNA. Translation: AAK76527.1.
AY117152 mRNA. Translation: AAM51227.1.
PIRiA55174.
RefSeqiNP_197429.1. NM_121933.3. [P46014-1]
UniGeneiAt.10199.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1MZKNMR-A180-313[»]
ProteinModelPortaliP46014.
SMRiP46014. Positions 180-298, 335-577.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi17324. 12 interactions.
IntActiP46014. 12 interactions.
MINTiMINT-98609.
STRINGi3702.AT5G19280.2.

Proteomic databases

PaxDbiP46014.
PRIDEiP46014.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G19280.1; AT5G19280.1; AT5G19280. [P46014-1]
GeneIDi832048.

Organism-specific databases

TAIRiAT5G19280.

Phylogenomic databases

eggNOGiCOG0631.
HOGENOMiHOG000240507.
InParanoidiP46014.
PhylomeDBiP46014.

Enzyme and pathway databases

BioCyciARA:AT5G19280-MONOMER.
ARA:GQT-2080-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP46014.
PROiP46014.

Gene expression databases

ExpressionAtlasiP46014. baseline and differential.

Family and domain databases

Gene3Di2.60.200.20. 1 hit.
3.60.40.10. 1 hit.
InterProiIPR000253. FHA_dom.
IPR016660. Kinase_assoc_Pase.
IPR015655. PP2C.
IPR000222. PP2C_BS.
IPR001932. PPM-type_phosphatase_dom.
IPR008984. SMAD_FHA_domain.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 1 hit.
PTHR13832:SF224. PTHR13832:SF224. 1 hit.
PfamiPF00498. FHA. 1 hit.
PF00481. PP2C. 1 hit.
[Graphical view]
PIRSFiPIRSF016465. Kap_phosphatase. 1 hit.
SMARTiSM00240. FHA. 1 hit.
SM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF49879. SSF49879. 1 hit.
SSF81606. SSF81606. 1 hit.
PROSITEiPS50006. FHA_DOMAIN. 1 hit.
PS01032. PPM_1. 1 hit.
PS51746. PPM_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Interaction of a protein phosphatase with an Arabidopsis serine-threonine receptor kinase."
    Stone J.M., Collinge M.A., Smith R.D., Horn M.A., Walker J.C.
    Science 266:793-795(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Stone J.M.
    Submitted (DEC-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  3. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
    Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
    , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
    Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "A possible role for kinase-associated protein phosphatase in the Arabidopsis CLAVATA1 signaling pathway."
    Williams R.W., Wilson J.M., Meyerowitz E.M.
    Proc. Natl. Acad. Sci. U.S.A. 94:10467-10472(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH CVL1.
  7. "Control of meristem development by CLAVATA1 receptor kinase and kinase-associated protein phosphatase interactions."
    Stone J.M., Trotochaud A.E., Walker J.C., Clark S.E.
    Plant Physiol. 117:1217-1225(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH CVL1.
  8. "The Arabidopsis kinase-associated protein phosphatase controls internalization of the somatic embryogenesis receptor kinase 1."
    Shah K., Russinova E., Gadella T.W. Jr., Willemse J., de Vries S.C.
    Genes Dev. 16:1707-1720(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  9. "The Arabidopsis SERK1 protein interacts with the AAA-ATPase AtCDC48, the 14-3-3 protein GF14lambda and the PP2C phosphatase KAPP."
    Rienties I.M., Vink J., Borst J.W., Russinova E., de Vries S.C.
    Planta 221:394-405(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH SERK1 AND CDC48A, IDENTIFICATION IN THE SERK1 COMPLEX.
  10. "Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis."
    Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y.
    BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  11. "The Arabidopsis kinase-associated protein phosphatase regulates adaptation to Na+ stress."
    Manabe Y., Bressan R.A., Wang T., Li F., Koiwa H., Sokolchik I., Li X., Maggio A.
    Plant Physiol. 146:612-622(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  12. "NMR structure of the forkhead-associated domain from the Arabidopsis receptor kinase-associated protein phosphatase."
    Lee G.I., Ding Z., Walker J.C., Van Doren S.R.
    Proc. Natl. Acad. Sci. U.S.A. 100:11261-11266(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 175-313.

Entry informationi

Entry nameiP2C70_ARATH
AccessioniPrimary (citable) accession number: P46014
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1997
Last modified: July 22, 2015
This is version 148 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.