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Protein

Protein phosphatase 2C 70

Gene

KAPP

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Dephosphorylates the Ser/Thr receptor-like kinase RLK5. May function as a signaling component in a pathway involving RLK5 (PubMed:15592873). Binds and dephosphorylates CLAVATA1 (CLV1). Functions as a negative regulator of the CLV1 signaling in plant development (PubMed:9294234, PubMed:9701578). Dephosphorylates SERK1 receptor kinase on threonine residues in the A-loop. Dephosphorylation of SERK1 controls SERK1 internalization (PubMed:12101128). Component of a signaling pathway which mediates adaptation to NaCl stress. Is not a component of the SALT OVERLY SENSITIVE (SOS) pathway (PubMed:18162596).5 Publications

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi346Manganese 1By similarity1
Metal bindingi346Manganese 2By similarity1
Metal bindingi347Manganese 1; via carbonyl oxygenBy similarity1
Metal bindingi521Manganese 2By similarity1
Metal bindingi568Manganese 2By similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Magnesium, Manganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase 2C 70 (EC:3.1.3.16)
Short name:
AtPP2C70
Alternative name(s):
Kinase-associated protein phosphatase1 Publication
Protein ROOT ATTENUATED GROWTH 11 Publication
Gene namesi
Name:KAPP1 Publication
Synonyms:RAG11 Publication
Ordered Locus Names:At5g19280
ORF Names:F7K24.30, T24G5.3
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G19280.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 7ExtracellularSequence analysis7
Transmembranei8 – 28HelicalSequence analysisAdd BLAST21
Topological domaini29 – 581CytoplasmicSequence analysisAdd BLAST553

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

No visible phenotype under normal growth conditions, but mutant seedlings show increased sensitivity of roots to salt stress.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000577801 – 581Protein phosphatase 2C 70Add BLAST581

Proteomic databases

PaxDbiP46014.

PTM databases

iPTMnetiP46014.

Expressioni

Tissue specificityi

Expressed in all tissues examined.

Gene expression databases

ExpressionAtlasiP46014. baseline and differential.
GenevisibleiP46014. AT.

Interactioni

Subunit structurei

Association of RLK5 with kapp domain is dependent on phosphorylation of RLK5 and can be abolished by dephosphorylation. Interacts with SERK1 and CDC48A. Component of the SERK1 signaling complex, composed of KAPP, CDC48A, GRF6 or GRF7, SERK1, SERK2, SERK3/BAK1 and BRI1 (PubMed:15592873). Interacts with CLV1 (PubMed:9294234, PubMed:9701578).3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CLV1Q9SYQ86EBI-1646157,EBI-1646111
CRK6Q9C5S92EBI-1646157,EBI-2023993
KIK1O499743EBI-1646157,EBI-2015790From a different organism.

Protein-protein interaction databases

BioGridi17324. 12 interactors.
IntActiP46014. 13 interactors.
MINTiMINT-98609.
STRINGi3702.AT5G19280.2.

Structurei

Secondary structure

1581
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi180 – 186Combined sources7
Beta strandi195 – 198Combined sources4
Beta strandi207 – 215Combined sources9
Beta strandi217 – 219Combined sources3
Beta strandi225 – 235Combined sources11
Turni236 – 239Combined sources4
Beta strandi240 – 245Combined sources6
Beta strandi257 – 260Combined sources4
Turni264 – 266Combined sources3
Beta strandi279 – 282Combined sources4
Beta strandi284 – 286Combined sources3
Beta strandi288 – 295Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MZKNMR-A180-313[»]
ProteinModelPortaliP46014.
SMRiP46014.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP46014.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini208 – 259FHAPROSITE-ProRule annotationAdd BLAST52
Domaini304 – 577PPM-type phosphatasePROSITE-ProRule annotationAdd BLAST274

Sequence similaritiesi

Contains 1 FHA domain.PROSITE-ProRule annotation
Contains 1 PPM-type phosphatase domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0698. Eukaryota.
COG0631. LUCA.
HOGENOMiHOG000240507.
InParanoidiP46014.
KOiK01090.
PhylomeDBiP46014.

Family and domain databases

Gene3Di2.60.200.20. 1 hit.
3.60.40.10. 1 hit.
InterProiIPR000253. FHA_dom.
IPR016660. Kinase_assoc_Pase.
IPR015655. PP2C.
IPR000222. PP2C_BS.
IPR001932. PPM-type_phosphatase_dom.
IPR008984. SMAD_FHA_domain.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 1 hit.
PTHR13832:SF224. PTHR13832:SF224. 1 hit.
PfamiPF00498. FHA. 1 hit.
PF00481. PP2C. 1 hit.
[Graphical view]
PIRSFiPIRSF016465. Kap_phosphatase. 1 hit.
SMARTiSM00240. FHA. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF49879. SSF49879. 1 hit.
SSF81606. SSF81606. 1 hit.
PROSITEiPS50006. FHA_DOMAIN. 1 hit.
PS01032. PPM_1. 1 hit.
PS51746. PPM_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: P46014-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAMIGMNIIG LFMVLMLLLI SLIILFACKP WRYFSRFRSS SRFSSTFKVG
60 70 80 90 100
DLQRPLISDD GNLIQGQTSE VTREYDLEGA CYQNDGLLHS SLTEGRFYKQ
110 120 130 140 150
RLPSSSPHFS QGESFVLEVI SEPSDNALVG QTLKLPAEKG SLAEVQTYDW
160 170 180 190 200
QNNRNENLQY NLEKDRLINL SPRLVEDQRS WLFLEVIAGP AIGLQHAVNS
210 220 230 240 250
TSSSKLPVKL GRVSPSDLAL KDSEVSGKHA QITWNSTKFK WELVDMGSLN
260 270 280 290 300
GTLVNSHSIS HPDLGSRKWG NPVELASDDI ITLGTTTKVY VRISSQNEFQ
310 320 330 340 350
IPFKIGVASD PMAMRRGGRK LPMEDVCHYK WPLPGANKFG LFCVCDGHGG
360 370 380 390 400
SGAAQSAIKI IPEVLANILS DSLRKEKVLS KRDASDVLRD MFAKTEARLE
410 420 430 440 450
EHQYEGCTAT VLLVWKDNEE NFFAQCANLG DSACVIHLGG RYIQMTEDHR
460 470 480 490 500
VVSLSERKRF QEAGLALRDG ETRLFGINLA RMLGDKFPKQ QDSRFSAEPY
510 520 530 540 550
ISEPLRIDQS SKDVFAVLAS DGLWDVVSPK KAVQLVLQMR DKERGRESSA
560 570 580
EKIANGLLNE ARAMRTKDNT SIIYLDFDTS L
Length:581
Mass (Da):64,911
Last modified:November 1, 1997 - v2
Checksum:iEE0C2A41E55E100D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09505 mRNA. Translation: AAB38148.1.
AC069326 Genomic DNA. No translation available.
AF296837 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED92679.1.
AY045853 mRNA. Translation: AAK76527.1.
AY117152 mRNA. Translation: AAM51227.1.
PIRiA55174.
RefSeqiNP_197429.1. NM_121933.4. [P46014-1]
UniGeneiAt.10199.

Genome annotation databases

EnsemblPlantsiAT5G19280.1; AT5G19280.1; AT5G19280. [P46014-1]
GeneIDi832048.
GrameneiAT5G19280.1; AT5G19280.1; AT5G19280.
KEGGiath:AT5G19280.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09505 mRNA. Translation: AAB38148.1.
AC069326 Genomic DNA. No translation available.
AF296837 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED92679.1.
AY045853 mRNA. Translation: AAK76527.1.
AY117152 mRNA. Translation: AAM51227.1.
PIRiA55174.
RefSeqiNP_197429.1. NM_121933.4. [P46014-1]
UniGeneiAt.10199.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MZKNMR-A180-313[»]
ProteinModelPortaliP46014.
SMRiP46014.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi17324. 12 interactors.
IntActiP46014. 13 interactors.
MINTiMINT-98609.
STRINGi3702.AT5G19280.2.

PTM databases

iPTMnetiP46014.

Proteomic databases

PaxDbiP46014.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G19280.1; AT5G19280.1; AT5G19280. [P46014-1]
GeneIDi832048.
GrameneiAT5G19280.1; AT5G19280.1; AT5G19280.
KEGGiath:AT5G19280.

Organism-specific databases

TAIRiAT5G19280.

Phylogenomic databases

eggNOGiKOG0698. Eukaryota.
COG0631. LUCA.
HOGENOMiHOG000240507.
InParanoidiP46014.
KOiK01090.
PhylomeDBiP46014.

Miscellaneous databases

EvolutionaryTraceiP46014.
PROiP46014.

Gene expression databases

ExpressionAtlasiP46014. baseline and differential.
GenevisibleiP46014. AT.

Family and domain databases

Gene3Di2.60.200.20. 1 hit.
3.60.40.10. 1 hit.
InterProiIPR000253. FHA_dom.
IPR016660. Kinase_assoc_Pase.
IPR015655. PP2C.
IPR000222. PP2C_BS.
IPR001932. PPM-type_phosphatase_dom.
IPR008984. SMAD_FHA_domain.
[Graphical view]
PANTHERiPTHR13832. PTHR13832. 1 hit.
PTHR13832:SF224. PTHR13832:SF224. 1 hit.
PfamiPF00498. FHA. 1 hit.
PF00481. PP2C. 1 hit.
[Graphical view]
PIRSFiPIRSF016465. Kap_phosphatase. 1 hit.
SMARTiSM00240. FHA. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMiSSF49879. SSF49879. 1 hit.
SSF81606. SSF81606. 1 hit.
PROSITEiPS50006. FHA_DOMAIN. 1 hit.
PS01032. PPM_1. 1 hit.
PS51746. PPM_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiP2C70_ARATH
AccessioniPrimary (citable) accession number: P46014
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1997
Last modified: November 30, 2016
This is version 157 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.