Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P46014

- P2C70_ARATH

UniProt

P46014 - P2C70_ARATH

Protein

Protein phosphatase 2C 70

Gene

KAPP

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 140 (01 Oct 2014)
      Sequence version 2 (01 Nov 1997)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Dephosphorylates the Ser/Thr receptor-like kinase RLK5. May function as a signaling component in a pathway involving RLK5.

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Cofactori

    Binds 2 magnesium or manganese ions per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi346 – 3461Manganese 1By similarity
    Metal bindingi346 – 3461Manganese 2By similarity
    Metal bindingi347 – 3471Manganese 1; via carbonyl oxygenBy similarity
    Metal bindingi521 – 5211Manganese 2By similarity
    Metal bindingi568 – 5681Manganese 2By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. protein binding Source: IntAct
    3. protein serine/threonine phosphatase activity Source: InterPro

    GO - Biological processi

    1. protein dephosphorylation Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Ligandi

    Magnesium, Manganese, Metal-binding

    Enzyme and pathway databases

    BioCyciARA:AT5G19280-MONOMER.
    ARA:GQT-2080-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein phosphatase 2C 70 (EC:3.1.3.16)
    Short name:
    AtPP2C70
    Alternative name(s):
    Kinase-associated protein phosphatase
    Gene namesi
    Name:KAPP
    Ordered Locus Names:At5g19280
    ORF Names:F7K24.30, T24G5.3
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G19280.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 581581Protein phosphatase 2C 70PRO_0000057780Add
    BLAST

    Proteomic databases

    PaxDbiP46014.
    PRIDEiP46014.

    Expressioni

    Tissue specificityi

    Expressed in all tissues examined.

    Gene expression databases

    ArrayExpressiP46014.
    GenevestigatoriP46014.

    Interactioni

    Subunit structurei

    Association of RLK5 with kapp domain is dependent on phosphorylation of RLK5 and can be abolished by dephosphorylation. Interacts with SERK1 and CDC48A. Component of the SERK1 signaling complex, composed of KAPP, CDC48A, GRF6 or GRF7, SERK1, SERK2, SERK3/BAK1 and BRI1.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CLV1Q9SYQ86EBI-1646157,EBI-1646111
    CRK6Q9C5S94EBI-1646157,EBI-2023993
    KIK1O499743EBI-1646157,EBI-2015790From a different organism.

    Protein-protein interaction databases

    BioGridi17324. 11 interactions.
    IntActiP46014. 12 interactions.
    MINTiMINT-98609.
    STRINGi3702.AT5G19280.2-P.

    Structurei

    Secondary structure

    1
    581
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi180 – 1867
    Beta strandi195 – 1984
    Beta strandi207 – 2159
    Beta strandi217 – 2193
    Beta strandi225 – 23511
    Turni236 – 2394
    Beta strandi240 – 2456
    Beta strandi257 – 2604
    Turni264 – 2663
    Beta strandi279 – 2824
    Beta strandi284 – 2863
    Beta strandi288 – 2958

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1MZKNMR-A180-313[»]
    ProteinModelPortaliP46014.
    SMRiP46014. Positions 180-298, 335-577.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP46014.

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 77ExtracellularSequence Analysis
    Topological domaini29 – 581553CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei8 – 2821HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini208 – 25952FHAPROSITE-ProRule annotationAdd
    BLAST
    Domaini289 – 581293PP2C-likeAdd
    BLAST

    Sequence similaritiesi

    Contains 1 FHA domain.PROSITE-ProRule annotation
    Contains 1 PP2C-like domain.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0631.
    HOGENOMiHOG000240507.
    InParanoidiP46014.
    KOiK01090.
    PhylomeDBiP46014.

    Family and domain databases

    Gene3Di2.60.200.20. 1 hit.
    3.60.40.10. 1 hit.
    InterProiIPR000253. FHA_dom.
    IPR016660. Kinase_assoc_Pase.
    IPR001932. PP2C-like_dom.
    IPR000222. PP2C_Mn2_Asp60_BS.
    IPR015655. Protein_Pase_2C.
    IPR008984. SMAD_FHA_domain.
    [Graphical view]
    PANTHERiPTHR13832. PTHR13832. 1 hit.
    PfamiPF00498. FHA. 1 hit.
    PF00481. PP2C. 1 hit.
    [Graphical view]
    PIRSFiPIRSF016465. Kap_phosphatase. 1 hit.
    SMARTiSM00240. FHA. 1 hit.
    SM00331. PP2C_SIG. 1 hit.
    SM00332. PP2Cc. 1 hit.
    [Graphical view]
    SUPFAMiSSF49879. SSF49879. 1 hit.
    SSF81606. SSF81606. 1 hit.
    PROSITEiPS50006. FHA_DOMAIN. 1 hit.
    PS01032. PP2C. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    This entry describes 1 isoform i produced by alternative splicing. Align

    Note: A number of isoforms are produced. According to EST sequences.

    Isoform 1 (identifier: P46014-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAMIGMNIIG LFMVLMLLLI SLIILFACKP WRYFSRFRSS SRFSSTFKVG    50
    DLQRPLISDD GNLIQGQTSE VTREYDLEGA CYQNDGLLHS SLTEGRFYKQ 100
    RLPSSSPHFS QGESFVLEVI SEPSDNALVG QTLKLPAEKG SLAEVQTYDW 150
    QNNRNENLQY NLEKDRLINL SPRLVEDQRS WLFLEVIAGP AIGLQHAVNS 200
    TSSSKLPVKL GRVSPSDLAL KDSEVSGKHA QITWNSTKFK WELVDMGSLN 250
    GTLVNSHSIS HPDLGSRKWG NPVELASDDI ITLGTTTKVY VRISSQNEFQ 300
    IPFKIGVASD PMAMRRGGRK LPMEDVCHYK WPLPGANKFG LFCVCDGHGG 350
    SGAAQSAIKI IPEVLANILS DSLRKEKVLS KRDASDVLRD MFAKTEARLE 400
    EHQYEGCTAT VLLVWKDNEE NFFAQCANLG DSACVIHLGG RYIQMTEDHR 450
    VVSLSERKRF QEAGLALRDG ETRLFGINLA RMLGDKFPKQ QDSRFSAEPY 500
    ISEPLRIDQS SKDVFAVLAS DGLWDVVSPK KAVQLVLQMR DKERGRESSA 550
    EKIANGLLNE ARAMRTKDNT SIIYLDFDTS L 581
    Length:581
    Mass (Da):64,911
    Last modified:November 1, 1997 - v2
    Checksum:iEE0C2A41E55E100D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U09505 mRNA. Translation: AAB38148.1.
    AC069326 Genomic DNA. No translation available.
    AF296837 Genomic DNA. No translation available.
    CP002688 Genomic DNA. Translation: AED92679.1.
    AY045853 mRNA. Translation: AAK76527.1.
    AY117152 mRNA. Translation: AAM51227.1.
    PIRiA55174.
    RefSeqiNP_197429.1. NM_121933.3. [P46014-1]
    UniGeneiAt.10199.

    Genome annotation databases

    EnsemblPlantsiAT5G19280.1; AT5G19280.1; AT5G19280. [P46014-1]
    GeneIDi832048.
    KEGGiath:AT5G19280.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U09505 mRNA. Translation: AAB38148.1 .
    AC069326 Genomic DNA. No translation available.
    AF296837 Genomic DNA. No translation available.
    CP002688 Genomic DNA. Translation: AED92679.1 .
    AY045853 mRNA. Translation: AAK76527.1 .
    AY117152 mRNA. Translation: AAM51227.1 .
    PIRi A55174.
    RefSeqi NP_197429.1. NM_121933.3. [P46014-1 ]
    UniGenei At.10199.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1MZK NMR - A 180-313 [» ]
    ProteinModelPortali P46014.
    SMRi P46014. Positions 180-298, 335-577.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 17324. 11 interactions.
    IntActi P46014. 12 interactions.
    MINTi MINT-98609.
    STRINGi 3702.AT5G19280.2-P.

    Proteomic databases

    PaxDbi P46014.
    PRIDEi P46014.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G19280.1 ; AT5G19280.1 ; AT5G19280 . [P46014-1 ]
    GeneIDi 832048.
    KEGGi ath:AT5G19280.

    Organism-specific databases

    TAIRi AT5G19280.

    Phylogenomic databases

    eggNOGi COG0631.
    HOGENOMi HOG000240507.
    InParanoidi P46014.
    KOi K01090.
    PhylomeDBi P46014.

    Enzyme and pathway databases

    BioCyci ARA:AT5G19280-MONOMER.
    ARA:GQT-2080-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P46014.

    Gene expression databases

    ArrayExpressi P46014.
    Genevestigatori P46014.

    Family and domain databases

    Gene3Di 2.60.200.20. 1 hit.
    3.60.40.10. 1 hit.
    InterProi IPR000253. FHA_dom.
    IPR016660. Kinase_assoc_Pase.
    IPR001932. PP2C-like_dom.
    IPR000222. PP2C_Mn2_Asp60_BS.
    IPR015655. Protein_Pase_2C.
    IPR008984. SMAD_FHA_domain.
    [Graphical view ]
    PANTHERi PTHR13832. PTHR13832. 1 hit.
    Pfami PF00498. FHA. 1 hit.
    PF00481. PP2C. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF016465. Kap_phosphatase. 1 hit.
    SMARTi SM00240. FHA. 1 hit.
    SM00331. PP2C_SIG. 1 hit.
    SM00332. PP2Cc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49879. SSF49879. 1 hit.
    SSF81606. SSF81606. 1 hit.
    PROSITEi PS50006. FHA_DOMAIN. 1 hit.
    PS01032. PP2C. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Interaction of a protein phosphatase with an Arabidopsis serine-threonine receptor kinase."
      Stone J.M., Collinge M.A., Smith R.D., Horn M.A., Walker J.C.
      Science 266:793-795(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. Stone J.M.
      Submitted (DEC-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION.
    3. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
      Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
      , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
      Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    4. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    6. "The Arabidopsis SERK1 protein interacts with the AAA-ATPase AtCDC48, the 14-3-3 protein GF14lambda and the PP2C phosphatase KAPP."
      Rienties I.M., Vink J., Borst J.W., Russinova E., de Vries S.C.
      Planta 221:394-405(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SERK1 AND CDC48A, IDENTIFICATION IN THE SERK1 COMPLEX.
    7. "Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis."
      Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y.
      BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.
    8. "NMR structure of the forkhead-associated domain from the Arabidopsis receptor kinase-associated protein phosphatase."
      Lee G.I., Ding Z., Walker J.C., Van Doren S.R.
      Proc. Natl. Acad. Sci. U.S.A. 100:11261-11266(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 175-313.

    Entry informationi

    Entry nameiP2C70_ARATH
    AccessioniPrimary (citable) accession number: P46014
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 140 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3