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Protein

Nitrilase 3

Gene

NIT3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Can convert indole-3-acetonitrile to the plant hormone indole-3-acetic acid.

Catalytic activityi

A nitrile + 2 H2O = a carboxylate + NH3.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei65Proton acceptor1
Active sitei152Proton donor1
Active sitei186Nucleophile1

GO - Molecular functioni

  • indole-3-acetonitrile nitrilase activity Source: TAIR
  • indole-3-acetonitrile nitrile hydratase activity Source: TAIR
  • nitrilase activity Source: TAIR

GO - Biological processi

  • cellular response to sulfate starvation Source: TAIR
  • glucosinolate catabolic process Source: TAIR
  • response to cadmium ion Source: TAIR

Keywordsi

Molecular functionHydrolase

Enzyme and pathway databases

BioCyciARA:AT3G44320-MONOMER.
MetaCyc:AT3G44320-MONOMER.
BRENDAi3.5.5.1. 399.

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrilase 3 (EC:3.5.5.1)
Gene namesi
Name:NIT3
Ordered Locus Names:At3g44320
ORF Names:T10D17_110
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G44320.
TAIRilocus:2095700. AT3G44320.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002040382 – 346Nitrilase 3Add BLAST345

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP46010.
PRIDEiP46010.

PTM databases

iPTMnetiP46010.

Expressioni

Gene expression databases

ExpressionAtlasiP46010. baseline and differential.
GenevisibleiP46010. AT.

Interactioni

Protein-protein interaction databases

BioGridi8877. 1 interactor.
MINTiMINT-8065341.
STRINGi3702.AT3G44320.1.

Structurei

3D structure databases

ProteinModelPortaliP46010.
SMRiP46010.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 297CN hydrolaseAdd BLAST273

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0805. Eukaryota.
COG0388. LUCA.
HOGENOMiHOG000256365.
InParanoidiP46010.
KOiK01501.
OMAiPNYESEG.
OrthoDBiEOG093613S7.
PhylomeDBiP46010.

Family and domain databases

Gene3Di3.60.110.10. 1 hit.
InterProiView protein in InterPro
IPR003010. C-N_Hydrolase.
IPR036526. C-N_Hydrolase_sf.
IPR000132. Nitrilase/CN_hydratase_CS.
PfamiView protein in Pfam
PF00795. CN_hydrolase. 1 hit.
SUPFAMiSSF56317. SSF56317. 1 hit.
PROSITEiView protein in PROSITE
PS50263. CN_HYDROLASE. 1 hit.
PS00920. NITRIL_CHT_1. 1 hit.
PS00921. NITRIL_CHT_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P46010-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSTEEMSSV KNTTQVIGVD PSSTVRVTIV QSSTVYNDTP ATLDKAEKFI
60 70 80 90 100
VEAASKGAKL VLFPEAFIGG YPRGFRFGLA VGVHNEEGRD EFRNYHASAI
110 120 130 140 150
KVPGPEVERL AELAGKNNVH LVMGAIEKDG YTLYCTALFF SPQGQFLGKH
160 170 180 190 200
RKVMPTSLER CIWGQGDGST IPVYDTPIGK IGAAICWENR MPLYRTALYA
210 220 230 240 250
KGIEIYCAPT ADYSLEWQAS MIHIAVEGGC FVLSAHQFCK RREFPEHPDY
260 270 280 290 300
LFNDIVDTKE HDPTVSGGGS VIISPLGKVL AGPNYESEGL VTADLDLGDI
310 320 330 340
ARAKLYFDVV GHYSKPDIFN LTVNEHPKKP VTFMTKVEKA EDESNK
Length:346
Mass (Da):38,022
Last modified:November 1, 1995 - v1
Checksum:i70CFF421547F2B5E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09959 mRNA. Translation: AAA19627.1.
Y07648 Genomic DNA. Translation: CAA68936.2.
AL353865 Genomic DNA. Translation: CAB89000.1.
CP002686 Genomic DNA. Translation: AEE77890.1.
BT002773 mRNA. Translation: AAO22601.1.
BT004345 mRNA. Translation: AAO42339.1.
PIRiT49148.
RefSeqiNP_190018.1. NM_114300.4.
UniGeneiAt.242.

Genome annotation databases

EnsemblPlantsiAT3G44320.1; AT3G44320.1; AT3G44320.
GeneIDi823557.
GrameneiAT3G44320.1; AT3G44320.1; AT3G44320.
KEGGiath:AT3G44320.

Similar proteinsi

Entry informationi

Entry nameiNRL3_ARATH
AccessioniPrimary (citable) accession number: P46010
Secondary accession number(s): O04909
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: October 25, 2017
This is version 136 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families