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Protein

Platelet-activating factor receptor

Gene

Ptafr

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Receptor for platelet activating factor, a chemotactic phospholipid mediator that possesses potent inflammatory, smooth-muscle contractile and hypotensive activity. Seems to mediate its action via a G protein that activates a phosphatidylinositol-calcium second messenger system.

GO - Molecular functioni

GO - Biological processi

  • cellular response to 2-O-acetyl-1-O-hexadecyl-sn-glycero-3-phosphocholine Source: RGD
  • cellular response to cAMP Source: RGD
  • cellular response to fatty acid Source: RGD
  • cellular response to gravity Source: RGD
  • chemotaxis Source: UniProtKB-KW
  • cytokine production Source: Ensembl
  • G-protein coupled receptor signaling pathway Source: RGD
  • inflammatory response Source: Ensembl
  • inositol trisphosphate biosynthetic process Source: Ensembl
  • negative regulation of blood pressure Source: RGD
  • parturition Source: RGD
  • phosphatidylinositol-mediated signaling Source: Ensembl
  • positive regulation of cellular extravasation Source: RGD
  • positive regulation of gastro-intestinal system smooth muscle contraction Source: RGD
  • positive regulation of inositol phosphate biosynthetic process Source: RGD
  • positive regulation of interleukin-6 biosynthetic process Source: RGD
  • positive regulation of leukocyte cell-cell adhesion Source: RGD
  • positive regulation of leukocyte tethering or rolling Source: RGD
  • positive regulation of maternal process involved in parturition Source: RGD
  • positive regulation of neutrophil degranulation Source: RGD
  • positive regulation of phospholipase C activity Source: RGD
  • positive regulation of sensory perception of pain Source: RGD
  • positive regulation of smooth muscle cell proliferation Source: RGD
  • positive regulation of smooth muscle contraction Source: RGD
  • positive regulation of transcytosis Source: RGD
  • positive regulation of translation Source: RGD
  • positive regulation of tumor necrosis factor production Source: RGD
  • positive regulation of vasoconstriction Source: RGD
  • positive regulation of vasodilation Source: RGD
  • positive regulation of voltage-gated chloride channel activity Source: RGD
  • regulation of transcription from RNA polymerase II promoter Source: RGD
  • response to 2-O-acetyl-1-O-hexadecyl-sn-glycero-3-phosphocholine Source: RGD
  • response to dexamethasone Source: RGD
  • response to lipopolysaccharide Source: RGD
  • response to organophosphorus Source: RGD
  • response to symbiotic bacterium Source: RGD
  • transcytosis Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Keywords - Biological processi

Chemotaxis

Enzyme and pathway databases

ReactomeiR-RNO-373076. Class A/1 (Rhodopsin-like receptors).
R-RNO-416476. G alpha (q) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Platelet-activating factor receptor
Short name:
PAF-R
Short name:
PAFr
Gene namesi
Name:Ptafr
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi61897. Ptafr.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1616ExtracellularSequence analysisAdd
BLAST
Transmembranei17 – 3822Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini39 – 5416CytoplasmicSequence analysisAdd
BLAST
Transmembranei55 – 7420Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini75 – 9117ExtracellularSequence analysisAdd
BLAST
Transmembranei92 – 11322Helical; Name=3Sequence analysisAdd
BLAST
Topological domaini114 – 13320CytoplasmicSequence analysisAdd
BLAST
Transmembranei134 – 15522Helical; Name=4Sequence analysisAdd
BLAST
Topological domaini156 – 18429ExtracellularSequence analysisAdd
BLAST
Transmembranei185 – 20521Helical; Name=5Sequence analysisAdd
BLAST
Topological domaini206 – 23328CytoplasmicSequence analysisAdd
BLAST
Transmembranei234 – 25421Helical; Name=6Sequence analysisAdd
BLAST
Topological domaini255 – 27521ExtracellularSequence analysisAdd
BLAST
Transmembranei276 – 29520Helical; Name=7Sequence analysisAdd
BLAST
Topological domaini296 – 34146CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of plasma membrane Source: GO_Central
  • membrane Source: RGD
  • nucleoplasm Source: Ensembl
  • plasma membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL4127.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 341341Platelet-activating factor receptorPRO_0000070096Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi4 – 41N-linked (GlcNAc...)Sequence analysis
Disulfide bondi90 ↔ 173PROSITE-ProRule annotation
Glycosylationi169 – 1691N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP46002.
PRIDEiP46002.

PTM databases

PhosphoSiteiP46002.

Expressioni

Tissue specificityi

Present in almost all organs including spleen, small intestine, kidney, lung, liver and brain.

Gene expression databases

GenevisibleiP46002. RN.

Interactioni

Subunit structurei

Interacts with ARRB1.By similarity

GO - Molecular functioni

  • mitogen-activated protein kinase binding Source: RGD

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000017687.

Chemistry

BindingDBiP46002.

Structurei

3D structure databases

ProteinModelPortaliP46002.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IIY4. Eukaryota.
ENOG410YB0R. LUCA.
GeneTreeiENSGT00640000091213.
HOGENOMiHOG000013041.
HOVERGENiHBG101106.
InParanoidiP46002.
KOiK04279.
OMAiFLCNLAG.
OrthoDBiEOG7QVM3C.
PhylomeDBiP46002.
TreeFamiTF350009.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR002282. PAF_rcpt.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR01153. PAFRECEPTOR.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P46002-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEQNGSFRVD SEFRYTLFPI VYSVIFVLGV VANGYVLWVF ATLYPSKKLN
60 70 80 90 100
EIKIFMVNLT VADLLFLMTL PLWIVYYSNE GDWIVHKFLC NLAGCLFFIN
110 120 130 140 150
TYCSVAFLGV ITYNRYQAVA YPIKTAQATT RKRGITLSLV IWISIAATAS
160 170 180 190 200
YFLATDSTNV VPKKDGSGNI TRCFEHYEPY SVPILVVHIF ITSCFFLVFF
210 220 230 240 250
LIFYCNMVII HTLLTRPVRQ QRKPEVKRRA LWMVCTVLAV FVICFVPHHV
260 270 280 290 300
VQLPWTLAEL GYQTNFHQAI NDAHQITLCL LSTNCVLDPV IYCFLTKKFR
310 320 330 340
KHLSEKFYSM RSSRKCSRAT SDTCTEVMMP ANQTPVLPLK N
Length:341
Mass (Da):39,204
Last modified:November 1, 1995 - v1
Checksum:iD0AF7290C3D34A5B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U04740 mRNA. Translation: AAA18422.1.
BC072491 mRNA. Translation: AAH72491.1.
PIRiS43252.
RefSeqiNP_445773.1. NM_053321.2.
XP_006239125.1. XM_006239063.2.
XP_006239126.1. XM_006239064.2.
UniGeneiRn.10137.

Genome annotation databases

EnsembliENSRNOT00000017687; ENSRNOP00000017687; ENSRNOG00000013231.
GeneIDi58949.
KEGGirno:58949.
UCSCiRGD:61897. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U04740 mRNA. Translation: AAA18422.1.
BC072491 mRNA. Translation: AAH72491.1.
PIRiS43252.
RefSeqiNP_445773.1. NM_053321.2.
XP_006239125.1. XM_006239063.2.
XP_006239126.1. XM_006239064.2.
UniGeneiRn.10137.

3D structure databases

ProteinModelPortaliP46002.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000017687.

Chemistry

BindingDBiP46002.
ChEMBLiCHEMBL4127.

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSiteiP46002.

Proteomic databases

PaxDbiP46002.
PRIDEiP46002.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000017687; ENSRNOP00000017687; ENSRNOG00000013231.
GeneIDi58949.
KEGGirno:58949.
UCSCiRGD:61897. rat.

Organism-specific databases

CTDi5724.
RGDi61897. Ptafr.

Phylogenomic databases

eggNOGiENOG410IIY4. Eukaryota.
ENOG410YB0R. LUCA.
GeneTreeiENSGT00640000091213.
HOGENOMiHOG000013041.
HOVERGENiHBG101106.
InParanoidiP46002.
KOiK04279.
OMAiFLCNLAG.
OrthoDBiEOG7QVM3C.
PhylomeDBiP46002.
TreeFamiTF350009.

Enzyme and pathway databases

ReactomeiR-RNO-373076. Class A/1 (Rhodopsin-like receptors).
R-RNO-416476. G alpha (q) signalling events.

Miscellaneous databases

PROiP46002.

Gene expression databases

GenevisibleiP46002. RN.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR002282. PAF_rcpt.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR01153. PAFRECEPTOR.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, expression and tissue distribution of rat platelet-activating-factor-receptor cDNA."
    Bito H., Honda Z., Nakamura M., Shimizu T.
    Eur. J. Biochem. 221:211-218(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Spleen.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung.

Entry informationi

Entry nameiPTAFR_RAT
AccessioniPrimary (citable) accession number: P46002
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: July 6, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.