P45982 (XYLB_BUTFI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Xylosidase/arabinosidase

Including the following 2 domains:

1. Beta-xylosidase
   EC=3.2.1.37
   Alternative name(s):
   1,4-beta-D-xylan xylohydrolase
   Xylan 1,4-beta-xylosidase
2. Alpha-N-arabinofuranosidase
   Short name=Arabinosidase
   EC=3.2.1.55

Gene names

Name:

xylB

Organism

Butyrivibrio fibrisolvens

Taxonomic identifier

831 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Clostridia › Clostridiales › Lachnospiraceae › Butyrivibrio

Protein attributes

Sequence length	517 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Has a 1.6-fold higher activity as an arabinosidase than as a beta-xylosidase when tested on the substrates nitrophenyl-beta-D-xylopyranoside and P-nitrophenyl-alpha-L-arabinofuranoside.
Catalytic activity	Hydrolysis of (1->4)-beta-D-xylans, to remove successive D-xylose residues from the non-reducing termini. Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides.
Sequence similarities	Belongs to the glycosyl hydrolase 43 family.

Ontologies

Keywords
Biological process	Xylan degradation
Molecular function	Glycosidase Hydrolase
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological_process	xylan catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	alpha-N-arabinofuranosidase activity Inferred from electronic annotation. Source: EC xylan 1,4-beta-xylosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 517	517	Xylosidase/arabinosidase		PRO_0000057694

Sequences

	Sequence		Length	Mass (Da)
	P45982 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: F1B645F76F6FE0B5	FASTA	517	58,222
	10 20 30 40 50 60 MVIANNPILK GFYPDPSICR KGDDFYLVCS SFVYAPGVPI FHTKDLAHFE QIGNILDRES 70 80 90 100 110 120 QLPLSGDISR GIFAPTIREH NGIFYMITTN VSSGGNFIVT AKDPAGPWSE PYYLGEDEAP 130 140 150 160 170 180 GIDPSLFFDD DGKCYYVGTR PNPDGVRYNG DWEIWVQELD LEQMKLVGPS MAIWKGALKD 190 200 210 220 230 240 VIWPEGPHLY KKDGYYYLLH AEAGTSFEHA ISVARSKELF KWFEGCPRNP IFTHRNLGKD 250 260 270 280 290 300 YPVCNVGHAD LVDDINGNWY MVMLASRPCK GKCSLGRETF LAKVIWEDGW PVVNPGVGRL 310 320 330 340 350 360 TDEVEMDLPE YRFSKEITTK DKMTFEETVL DDRFVGIERR SEDFYSLTDN PGFLRLKLRP 370 380 390 400 410 420 EAIENTGNPS YLGIRQKTHS FRASCGLKFT PAKDNECAGM VLFQNNENHL ELLVVKKKDK 430 440 450 460 470 480 LQFKVGPVIK GTKIRLATFD ISSGDLEIIL EAANQLANIY IKKNNEKILV AECIDLSPYT 490 500 510 TEESGGFVGC TIGLYASSNG KTSDNYCDYS YFTVEEV

References

[1]

"Sequencing and expression of the Butyrivibrio fibrisolvens xylB gene encoding a novel bifunctional protein with beta-D-xylosidase and alpha-L-arabinofuranosidase activities." Utt E.A., Eddy C.K., Keshav K.F., Ingram L.O. Appl. Environ. Microbiol. 57:1227-1234(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	M55537 Genomic DNA. Translation: AAA63610.1.
PIR	A49776.
3D structure databases
ProteinModelPortal	P45982.
ModBase	Search...
Protein family/group databases
CAZy	GH43. Glycoside Hydrolase Family 43.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	2.115.10.20. 1 hit. 2.60.120.200. 1 hit.
InterPro	IPR008985. ConA-like_lec_gl_sf. IPR013320. ConA-like_subgrp. IPR006710. Glyco_hydro_43. IPR023296. Glyco_hydro_beta-prop. [Graphical view]
PANTHER	PTHR22925. PTHR22925. 1 hit.
Pfam	PF04616. Glyco_hydro_43. 1 hit. [Graphical view]
SUPFAM	SSF49899. ConA_like_lec_gl. 1 hit. SSF75005. Glyco_hydro_43_beta-prop. 1 hit.
ProtoNet	Search...

Entry information

Entry name

XYLB_BUTFI

Accession

Primary (citable) accession number: P45982

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	April 3, 2013
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program