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Protein

Pre-mRNA polyadenylation factor FIP1

Gene

FIP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Polymerase-regulating component of the cleavage and polyadenylation factor (CPF) complex, which plays a key role in polyadenylation-dependent pre-mRNA 3'-end formation and cooperates with cleavage factors including the CFIA complex and NAB4/CFIB. Pre-mRNA polyadenylation factor that directly interacts with poly(A) polymerase PAP1. This inhibits the extension of an oligo(A) primer by limiting access of the RNA substrate to the C-terminal RNA binding domain of PAP1. Seems to tether PAP1 to the cleavage factor I.1 Publication

GO - Molecular functioni

  • protein binding, bridging Source: SGD

GO - Biological processi

  • mRNA polyadenylation Source: SGD
  • pre-mRNA cleavage required for polyadenylation Source: SGD
Complete GO annotation...

Keywords - Biological processi

mRNA processing

Enzyme and pathway databases

BioCyciYEAST:G3O-31720-MONOMER.
ReactomeiR-SCE-77595. Processing of Intronless Pre-mRNAs.

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA polyadenylation factor FIP1
Gene namesi
Name:FIP1
Ordered Locus Names:YJR093C
ORF Names:J1911
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJR093C.
SGDiS000003853. FIP1.

Subcellular locationi

GO - Cellular componenti

  • mRNA cleavage and polyadenylation specificity factor complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 327327Pre-mRNA polyadenylation factor FIP1PRO_0000215042Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei50 – 501PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP45976.

PTM databases

iPTMnetiP45976.

Interactioni

Subunit structurei

Component of the cleavage and polyadenylation factor (CPF) complex, which is composed of PTI1, SYC1, SSU72, GLC7, MPE1, REF2, PFS2, PTA1, YSH1/BRR5, SWD2, CFT2/YDH1, YTH1, CFT1/YHH1, FIP1 and PAP1. In the CPF complex probably interacts directly with PAP1 and YTH1. Interacts with RNA14.6 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PAP1P2946811EBI-6940,EBI-12917
YTH1Q061027EBI-6940,EBI-38049

GO - Molecular functioni

  • protein binding, bridging Source: SGD

Protein-protein interaction databases

BioGridi33847. 44 interactions.
DIPiDIP-2029N.
IntActiP45976. 37 interactions.
MINTiMINT-383835.

Structurei

Secondary structure

1
327
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi83 – 853
Beta strandi87 – 904
Beta strandi93 – 953
Helixi96 – 983
Helixi99 – 1024

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3C66X-ray2.60C/D80-105[»]
DisProtiDP00625.
ProteinModelPortaliP45976.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP45976.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi61 – 8020Asp-rich (acidic)Add
BLAST
Compositional biasi258 – 2669Poly-Asn
Compositional biasi284 – 31835Pro-richAdd
BLAST

Domaini

Circular dichroism measurements suggest that the protein is largely unstructured in the absence of interaction with PAP1.1 Publication

Sequence similaritiesi

Belongs to the FIP1 family.Curated

Phylogenomic databases

GeneTreeiENSGT00730000111028.
HOGENOMiHOG000000819.
InParanoidiP45976.
KOiK14405.
OMAiKEKPWRQ.
OrthoDBiEOG092C4SHL.

Family and domain databases

InterProiIPR007854. Fip1.
[Graphical view]
PfamiPF05182. Fip1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P45976-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSSEDEDDK FLYGSDSELA LPSSKRSRDD EADAGASSNP DIVKRQKFDS
60 70 80 90 100
PVEETPATAR DDRSDEDIYS DSSDDDSDSD LEVIISLGPD PTRLDAKLLD
110 120 130 140 150
SYSTAATSSS KDVISVATDV SNTITKTSDE RLITEGEANQ GVTATTVKAT
160 170 180 190 200
ESDGNVPKAM TGSIDLDKEG IFDSVGITTI DPEVLKEKPW RQPGANLSDY
210 220 230 240 250
FNYGFNEFTW MEYLHRQEKL QQDYNPRRIL MGLLSLQQQG KLNSANDTDS
260 270 280 290 300
NLGNIIDNNN NVNNANMSNL NSNMGNSMSG TPNPPAPPMH PSFPPLPMFG
310 320
SFPPFPMPGM MPPMNQQPNQ NQNQNSK
Length:327
Mass (Da):35,777
Last modified:November 1, 1995 - v1
Checksum:i471CA2B0CDF99D0A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X83796 Genomic DNA. Translation: CAA58727.1.
Z49593 Genomic DNA. Translation: CAA89621.1.
BK006943 Genomic DNA. Translation: DAA08877.1.
PIRiA56545.
RefSeqiNP_012626.1. NM_001181750.1.

Genome annotation databases

EnsemblFungiiYJR093C; YJR093C; YJR093C.
GeneIDi853555.
KEGGisce:YJR093C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X83796 Genomic DNA. Translation: CAA58727.1.
Z49593 Genomic DNA. Translation: CAA89621.1.
BK006943 Genomic DNA. Translation: DAA08877.1.
PIRiA56545.
RefSeqiNP_012626.1. NM_001181750.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3C66X-ray2.60C/D80-105[»]
DisProtiDP00625.
ProteinModelPortaliP45976.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33847. 44 interactions.
DIPiDIP-2029N.
IntActiP45976. 37 interactions.
MINTiMINT-383835.

PTM databases

iPTMnetiP45976.

Proteomic databases

MaxQBiP45976.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJR093C; YJR093C; YJR093C.
GeneIDi853555.
KEGGisce:YJR093C.

Organism-specific databases

EuPathDBiFungiDB:YJR093C.
SGDiS000003853. FIP1.

Phylogenomic databases

GeneTreeiENSGT00730000111028.
HOGENOMiHOG000000819.
InParanoidiP45976.
KOiK14405.
OMAiKEKPWRQ.
OrthoDBiEOG092C4SHL.

Enzyme and pathway databases

BioCyciYEAST:G3O-31720-MONOMER.
ReactomeiR-SCE-77595. Processing of Intronless Pre-mRNAs.

Miscellaneous databases

EvolutionaryTraceiP45976.
PROiP45976.

Family and domain databases

InterProiIPR007854. Fip1.
[Graphical view]
PfamiPF05182. Fip1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFIP1_YEAST
AccessioniPrimary (citable) accession number: P45976
Secondary accession number(s): D6VWR1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: September 7, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1310 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.