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P45976

- FIP1_YEAST

UniProt

P45976 - FIP1_YEAST

Protein

Pre-mRNA polyadenylation factor FIP1

Gene

FIP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 106 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Polymerase-regulating component of the cleavage and polyadenylation factor (CPF) complex, which plays a key role in polyadenylation-dependent pre-mRNA 3'-end formation and cooperates with cleavage factors including the CFIA complex and NAB4/CFIB. Pre-mRNA polyadenylation factor that directly interacts with poly(A) polymerase PAP1. This inhibits the extension of an oligo(A) primer by limiting access of the RNA substrate to the C-terminal RNA binding domain of PAP1. Seems to tether PAP1 to the cleavage factor I.1 Publication

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. protein binding, bridging Source: SGD

    GO - Biological processi

    1. mRNA cleavage Source: SGD
    2. mRNA polyadenylation Source: SGD

    Keywords - Biological processi

    mRNA processing

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31720-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pre-mRNA polyadenylation factor FIP1
    Gene namesi
    Name:FIP1
    Ordered Locus Names:YJR093C
    ORF Names:J1911
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome X

    Organism-specific databases

    CYGDiYJR093c.
    SGDiS000003853. FIP1.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. mRNA cleavage and polyadenylation specificity factor complex Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 327327Pre-mRNA polyadenylation factor FIP1PRO_0000215042Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei50 – 501Phosphoserine2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP45976.
    PaxDbiP45976.

    Expressioni

    Gene expression databases

    GenevestigatoriP45976.

    Interactioni

    Subunit structurei

    Component of the cleavage and polyadenylation factor (CPF) complex, which is composed of PTI1, SYC1, SSU72, GLC7, MPE1, REF2, PFS2, PTA1, YSH1/BRR5, SWD2, CFT2/YDH1, YTH1, CFT1/YHH1, FIP1 and PAP1. In the CPF complex probably interacts directly with PAP1 and YTH1. Interacts with RNA14.6 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    PAP1P2946811EBI-6940,EBI-12917
    YTH1Q061027EBI-6940,EBI-38049

    Protein-protein interaction databases

    BioGridi33847. 42 interactions.
    DIPiDIP-2029N.
    IntActiP45976. 36 interactions.
    MINTiMINT-383835.
    STRINGi4932.YJR093C.

    Structurei

    Secondary structure

    1
    327
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi83 – 853
    Beta strandi87 – 904
    Beta strandi93 – 953
    Helixi96 – 983
    Helixi99 – 1024

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3C66X-ray2.60C/D80-105[»]
    DisProtiDP00625.
    ProteinModelPortaliP45976.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP45976.

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi61 – 8020Asp-rich (acidic)Add
    BLAST
    Compositional biasi258 – 2669Poly-Asn
    Compositional biasi284 – 31835Pro-richAdd
    BLAST

    Domaini

    Circular dichroism measurements suggest that the protein is largely unstructured in the absence of interaction with PAP1.1 Publication

    Sequence similaritiesi

    Belongs to the FIP1 family.Curated

    Phylogenomic databases

    eggNOGiCOG5213.
    GeneTreeiENSGT00730000111028.
    HOGENOMiHOG000000819.
    KOiK14405.
    OMAiTWMEYLH.
    OrthoDBiEOG7SFJ6V.

    Family and domain databases

    InterProiIPR007854. Fip1.
    [Graphical view]
    PfamiPF05182. Fip1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P45976-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSSEDEDDK FLYGSDSELA LPSSKRSRDD EADAGASSNP DIVKRQKFDS    50
    PVEETPATAR DDRSDEDIYS DSSDDDSDSD LEVIISLGPD PTRLDAKLLD 100
    SYSTAATSSS KDVISVATDV SNTITKTSDE RLITEGEANQ GVTATTVKAT 150
    ESDGNVPKAM TGSIDLDKEG IFDSVGITTI DPEVLKEKPW RQPGANLSDY 200
    FNYGFNEFTW MEYLHRQEKL QQDYNPRRIL MGLLSLQQQG KLNSANDTDS 250
    NLGNIIDNNN NVNNANMSNL NSNMGNSMSG TPNPPAPPMH PSFPPLPMFG 300
    SFPPFPMPGM MPPMNQQPNQ NQNQNSK 327
    Length:327
    Mass (Da):35,777
    Last modified:November 1, 1995 - v1
    Checksum:i471CA2B0CDF99D0A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X83796 Genomic DNA. Translation: CAA58727.1.
    Z49593 Genomic DNA. Translation: CAA89621.1.
    BK006943 Genomic DNA. Translation: DAA08877.1.
    PIRiA56545.
    RefSeqiNP_012626.1. NM_001181750.1.

    Genome annotation databases

    EnsemblFungiiYJR093C; YJR093C; YJR093C.
    GeneIDi853555.
    KEGGisce:YJR093C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X83796 Genomic DNA. Translation: CAA58727.1 .
    Z49593 Genomic DNA. Translation: CAA89621.1 .
    BK006943 Genomic DNA. Translation: DAA08877.1 .
    PIRi A56545.
    RefSeqi NP_012626.1. NM_001181750.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3C66 X-ray 2.60 C/D 80-105 [» ]
    DisProti DP00625.
    ProteinModelPortali P45976.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33847. 42 interactions.
    DIPi DIP-2029N.
    IntActi P45976. 36 interactions.
    MINTi MINT-383835.
    STRINGi 4932.YJR093C.

    Proteomic databases

    MaxQBi P45976.
    PaxDbi P45976.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YJR093C ; YJR093C ; YJR093C .
    GeneIDi 853555.
    KEGGi sce:YJR093C.

    Organism-specific databases

    CYGDi YJR093c.
    SGDi S000003853. FIP1.

    Phylogenomic databases

    eggNOGi COG5213.
    GeneTreei ENSGT00730000111028.
    HOGENOMi HOG000000819.
    KOi K14405.
    OMAi TWMEYLH.
    OrthoDBi EOG7SFJ6V.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31720-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P45976.
    NextBioi 974296.
    PROi P45976.

    Gene expression databases

    Genevestigatori P45976.

    Family and domain databases

    InterProi IPR007854. Fip1.
    [Graphical view ]
    Pfami PF05182. Fip1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The FIP1 gene encodes a component of a yeast pre-mRNA polyadenylation factor that directly interacts with poly(A) polymerase."
      Preker P.J., Lingner J., Minvielle-Sebastia L., Keller W.
      Cell 81:379-389(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INTERACTION WITH PAP1.
    2. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
      Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
      , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
      EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "The WD-repeat protein pfs2p bridges two essential factors within the yeast pre-mRNA 3'-end-processing complex."
      Ohnacker M., Barabino S.M.L., Preker P.J., Keller W.
      EMBO J. 19:37-47(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PFS2; RNA14 AND YSH1.
    5. "Fip1 regulates the activity of Poly(A) polymerase through multiple interactions."
      Helmling S., Zhelkovsky A., Moore C.L.
      Mol. Cell. Biol. 21:2026-2037(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH PAP1 AND YTH1.
    6. "Organization and function of APT, a subcomplex of the yeast cleavage and polyadenylation factor involved in the formation of mRNA and small nucleolar RNA 3'-ends."
      Nedea E., He X., Kim M., Pootoolal J., Zhong G., Canadien V., Hughes T., Buratowski S., Moore C.L., Greenblatt J.
      J. Biol. Chem. 278:33000-33010(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE CPF COMPLEX, SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY.
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. "Functional dissection of the zinc finger and flanking domains of the Yth1 cleavage/polyadenylation factor."
      Tacahashi Y., Helmling S., Moore C.L.
      Nucleic Acids Res. 31:1744-1752(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH YTH1.
    9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-50, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-50, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. "Structure of yeast poly(A) polymerase in complex with a peptide from Fip1, an intrinsically disordered protein."
      Meinke G., Ezeokonkwo C., Balbo P., Stafford W., Moore C., Bohm A.
      Biochemistry 47:6859-6869(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) OF 80-105 IN COMPLEX WITH PAP1, SUBUNIT, DOMAIN, CIRCULAR DICHROISM.

    Entry informationi

    Entry nameiFIP1_YEAST
    AccessioniPrimary (citable) accession number: P45976
    Secondary accession number(s): D6VWR1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 106 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1310 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome X
      Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

    External Data

    Dasty 3