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Protein

Chromobox protein homolog 5

Gene

CBX5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Host-virus interaction

Enzyme and pathway databases

BioCyciZFISH:ENSG00000094916-MONOMER.
ReactomeiR-HSA-983231. Factors involved in megakaryocyte development and platelet production.
SIGNORiP45973.

Names & Taxonomyi

Protein namesi
Recommended name:
Chromobox protein homolog 5
Alternative name(s):
Antigen p25
Heterochromatin protein 1 homolog alpha
Short name:
HP1 alpha
Gene namesi
Name:CBX5
Synonyms:HP1A
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

HGNCiHGNC:1555. CBX5.

Subcellular locationi

GO - Cellular componenti

  • chromocenter Source: Ensembl
  • histone deacetylase complex Source: BHF-UCL
  • histone methyltransferase complex Source: BHF-UCL
  • kinetochore Source: Ensembl
  • nuclear chromosome, telomeric region Source: BHF-UCL
  • nuclear envelope Source: ProtInc
  • nuclear heterochromatin Source: ProtInc
  • nuclear pericentric heterochromatin Source: BHF-UCL
  • nucleolus Source: BHF-UCL
  • nucleoplasm Source: Reactome
  • nucleus Source: UniProtKB
  • pericentric heterochromatin Source: BHF-UCL
  • transcriptional repressor complex Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi165I → E: No effect on interaction with POGZ. Abolishes interaction with TRIM28, CHAF1A and NIPBL. 1 Publication1
Mutagenesisi174W → A: Abolishes interaction with TRIM28, CHAF1A, NIPBL and HP1BP3, fails to localize to centromeres in mitosis. 3 Publications1

Organism-specific databases

DisGeNETi23468.
OpenTargetsiENSG00000094916.
PharmGKBiPA26130.

Polymorphism and mutation databases

BioMutaiCBX5.
DMDMi1170338.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000802081 – 191Chromobox protein homolog 5Add BLAST191

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei11PhosphoserineCombined sources1
Modified residuei12PhosphoserineCombined sources1
Modified residuei13PhosphoserineCombined sources1
Modified residuei14PhosphoserineCombined sources1
Cross-linki32Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei92PhosphoserineCombined sources1
Modified residuei95PhosphoserineCombined sources1
Modified residuei97PhosphoserineCombined sources1
Cross-linki102Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources

Post-translational modificationi

Phosphorylation of HP1 and LBR may be responsible for some of the alterations in chromatin organization and nuclear structure which occur at various times during the cell cycle (By similarity). Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis.By similarity1 Publication
Ubiquitinated.1 Publication

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP45973.
PaxDbiP45973.
PeptideAtlasiP45973.
PRIDEiP45973.
TopDownProteomicsiP45973.

PTM databases

iPTMnetiP45973.
PhosphoSitePlusiP45973.

Expressioni

Gene expression databases

BgeeiENSG00000094916.
CleanExiHS_CBX5.
ExpressionAtlasiP45973. baseline and differential.
GenevisibleiP45973. HS.

Organism-specific databases

HPAiCAB017548.
HPA016699.

Interactioni

Subunit structurei

Interacts with KMT5B and KMT5C (By similarity). Interacts with HP1BP3. Interacts directly with ATRX, CHAF1A, LBR, NIPBL, SP100, STAM2 and TRIM28 via the chromoshadow domain. Can interact directly with CBX3 via the chromoshadow domain. Interacts with histone H3 methylated at 'Lys-9'. Interacts with BAHD1, MIS12 and DSN1. Interacts with POGZ; POGZ and PXVXL motif-containing proteins such as INCENP and TRIM28 compete for interaction with CBX5. Interacts with INCENP and TRIM24. Interacts with JC virus agnoprotein; this interaction induces the dissociation of CBX5 from LBR, resulting in destabilization of the nuclear envelope. Interacts with CHAMP1. Interacts with ASXL1.By similarity14 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ADNPQ9H2P03EBI-78219,EBI-1764854
ASXL1Q8IXJ92EBI-78219,EBI-1646500
Asxl1P595985EBI-78219,EBI-5743705From a different organism.
ATRXP461002EBI-78219,EBI-396461
CHAF1AQ131118EBI-78219,EBI-1020839
H3F3BP842432EBI-78219,EBI-120658
HIST1H3DP684319EBI-78219,EBI-79722
INCENPQ9NQS76EBI-78219,EBI-307907
LBRQ147394EBI-78219,EBI-1055147
legAS4Q5ZUS46EBI-78219,EBI-8871796From a different organism.
LRIF1Q5T3J33EBI-78219,EBI-473196
MBD1Q9UIS96EBI-78219,EBI-867196
POGZQ7Z3K37EBI-78219,EBI-1389308
PRR14Q9BWN13EBI-78219,EBI-748167
SENP7Q9BQF64EBI-78219,EBI-766251
SP100P23497-26EBI-78219,EBI-6589365
SUV39H1O434636EBI-78219,EBI-349968
TRIM28Q1326310EBI-78219,EBI-78139

GO - Molecular functioni

Protein-protein interaction databases

BioGridi117030. 177 interactors.
DIPiDIP-5986N.
IntActiP45973. 150 interactors.
MINTiMINT-1178082.
STRINGi9606.ENSP00000209875.

Structurei

Secondary structure

1191
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi19 – 31Combined sources13
Beta strandi34 – 41Combined sources8
Helixi46 – 48Combined sources3
Beta strandi50 – 53Combined sources4
Helixi54 – 56Combined sources3
Helixi60 – 67Combined sources8
Helixi116 – 119Combined sources4
Beta strandi123 – 130Combined sources8
Beta strandi137 – 142Combined sources6
Beta strandi148 – 152Combined sources5
Helixi153 – 159Combined sources7
Helixi161 – 168Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3FDTX-ray2.00A18-75[»]
3I3CX-ray2.48A/B/C/D110-173[»]
ProteinModelPortaliP45973.
SMRiP45973.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP45973.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini20 – 78Chromo 1PROSITE-ProRule annotationAdd BLAST59
Domaini121 – 179Chromo 2; shadow subtypePROSITE-ProRule annotationAdd BLAST59

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni116 – 191Interaction with ASXL11 PublicationAdd BLAST76

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi11 – 14Poly-Ser4

Sequence similaritiesi

Contains 2 chromo domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IUAB. Eukaryota.
ENOG4111I3N. LUCA.
GeneTreeiENSGT00510000046310.
HOGENOMiHOG000220852.
HOVERGENiHBG000400.
InParanoidiP45973.
KOiK11587.
OMAiWHAYPEE.
OrthoDBiEOG091G0RBS.
PhylomeDBiP45973.
TreeFamiTF350503.

Family and domain databases

InterProiIPR000953. Chromo/shadow_dom.
IPR017984. Chromo_dom_subgr.
IPR023780. Chromo_domain.
IPR008251. Chromo_shadow_dom.
IPR016197. Chromodomain-like.
IPR023779. Chromodomain_CS.
[Graphical view]
PfamiPF00385. Chromo. 1 hit.
PF01393. Chromo_shadow. 1 hit.
[Graphical view]
PRINTSiPR00504. CHROMODOMAIN.
SMARTiSM00298. CHROMO. 2 hits.
SM00300. ChSh. 1 hit.
[Graphical view]
SUPFAMiSSF54160. SSF54160. 2 hits.
PROSITEiPS00598. CHROMO_1. 1 hit.
PS50013. CHROMO_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P45973-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKKTKRTAD SSSSEDEEEY VVEKVLDRRV VKGQVEYLLK WKGFSEEHNT
60 70 80 90 100
WEPEKNLDCP ELISEFMKKY KKMKEGENNK PREKSESNKR KSNFSNSADD
110 120 130 140 150
IKSKKKREQS NDIARGFERG LEPEKIIGAT DSCGDLMFLM KWKDTDEADL
160 170 180 190
VLAKEANVKC PQIVIAFYEE RLTWHAYPED AENKEKETAK S
Length:191
Mass (Da):22,225
Last modified:November 1, 1995 - v1
Checksum:i16FFC476367093B1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S62077 mRNA. Translation: AAB26994.1.
L07515 mRNA. Translation: AAA72327.1.
AK313506 mRNA. Translation: BAG36286.1.
CH471054 Genomic DNA. Translation: EAW96759.1.
BC006821 mRNA. Translation: AAH06821.1.
U26311 mRNA. Translation: AAC50553.1.
CCDSiCCDS8875.1.
PIRiG01808.
RefSeqiNP_001120793.1. NM_001127321.1.
NP_001120794.1. NM_001127322.1.
NP_036249.1. NM_012117.2.
UniGeneiHs.349283.

Genome annotation databases

EnsembliENST00000209875; ENSP00000209875; ENSG00000094916.
ENST00000439541; ENSP00000401009; ENSG00000094916.
ENST00000550411; ENSP00000449207; ENSG00000094916.
GeneIDi23468.
KEGGihsa:23468.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S62077 mRNA. Translation: AAB26994.1.
L07515 mRNA. Translation: AAA72327.1.
AK313506 mRNA. Translation: BAG36286.1.
CH471054 Genomic DNA. Translation: EAW96759.1.
BC006821 mRNA. Translation: AAH06821.1.
U26311 mRNA. Translation: AAC50553.1.
CCDSiCCDS8875.1.
PIRiG01808.
RefSeqiNP_001120793.1. NM_001127321.1.
NP_001120794.1. NM_001127322.1.
NP_036249.1. NM_012117.2.
UniGeneiHs.349283.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3FDTX-ray2.00A18-75[»]
3I3CX-ray2.48A/B/C/D110-173[»]
ProteinModelPortaliP45973.
SMRiP45973.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117030. 177 interactors.
DIPiDIP-5986N.
IntActiP45973. 150 interactors.
MINTiMINT-1178082.
STRINGi9606.ENSP00000209875.

PTM databases

iPTMnetiP45973.
PhosphoSitePlusiP45973.

Polymorphism and mutation databases

BioMutaiCBX5.
DMDMi1170338.

Proteomic databases

EPDiP45973.
PaxDbiP45973.
PeptideAtlasiP45973.
PRIDEiP45973.
TopDownProteomicsiP45973.

Protocols and materials databases

DNASUi23468.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000209875; ENSP00000209875; ENSG00000094916.
ENST00000439541; ENSP00000401009; ENSG00000094916.
ENST00000550411; ENSP00000449207; ENSG00000094916.
GeneIDi23468.
KEGGihsa:23468.

Organism-specific databases

CTDi23468.
DisGeNETi23468.
GeneCardsiCBX5.
HGNCiHGNC:1555. CBX5.
HPAiCAB017548.
HPA016699.
MIMi604478. gene.
neXtProtiNX_P45973.
OpenTargetsiENSG00000094916.
PharmGKBiPA26130.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IUAB. Eukaryota.
ENOG4111I3N. LUCA.
GeneTreeiENSGT00510000046310.
HOGENOMiHOG000220852.
HOVERGENiHBG000400.
InParanoidiP45973.
KOiK11587.
OMAiWHAYPEE.
OrthoDBiEOG091G0RBS.
PhylomeDBiP45973.
TreeFamiTF350503.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000094916-MONOMER.
ReactomeiR-HSA-983231. Factors involved in megakaryocyte development and platelet production.
SIGNORiP45973.

Miscellaneous databases

ChiTaRSiCBX5. human.
EvolutionaryTraceiP45973.
GeneWikiiCBX5_(gene).
GenomeRNAii23468.
PROiP45973.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000094916.
CleanExiHS_CBX5.
ExpressionAtlasiP45973. baseline and differential.
GenevisibleiP45973. HS.

Family and domain databases

InterProiIPR000953. Chromo/shadow_dom.
IPR017984. Chromo_dom_subgr.
IPR023780. Chromo_domain.
IPR008251. Chromo_shadow_dom.
IPR016197. Chromodomain-like.
IPR023779. Chromodomain_CS.
[Graphical view]
PfamiPF00385. Chromo. 1 hit.
PF01393. Chromo_shadow. 1 hit.
[Graphical view]
PRINTSiPR00504. CHROMODOMAIN.
SMARTiSM00298. CHROMO. 2 hits.
SM00300. ChSh. 1 hit.
[Graphical view]
SUPFAMiSSF54160. SSF54160. 2 hits.
PROSITEiPS00598. CHROMO_1. 1 hit.
PS50013. CHROMO_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCBX5_HUMAN
AccessioniPrimary (citable) accession number: P45973
Secondary accession number(s): B2R8T9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 30, 2016
This is version 171 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.