P45971 (OST48_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit
Short name=Oligosaccharyl transferase 48 kDa subunit
EC=2.4.99.18

Gene names

ORF Names:

T09A5.11

Organism

Caenorhabditis elegans [Reference proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	445 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains By similarity.
Catalytic activity	Dolichyl diphosphooligosaccharide + [protein]-L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine.
Pathway	Protein modification; protein glycosylation.
Subunit structure	Component of the oligosaccharyltransferase (OST) complex By similarity.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type I membrane protein By similarity.
Sequence similarities	Belongs to the DDOST 48 kDa subunit family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Signal Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein N-linked glycosylation via asparagine Inferred from electronic annotation. Source: InterPro
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	dolichyl-diphosphooligosaccharide-protein glycotransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 445	425	Probable dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit		PRO_0000021954
Regions
	Topological domain	21 – 405	385	Lumenal Potential
	Transmembrane	406 – 426	21	Helical; Potential
	Topological domain	427 – 445	19	Cytoplasmic Potential

Sequences

	Sequence		Length	Mass (Da)
	P45971 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 59A14E6ECF43F0F9	FASTA	445	48,760
	10 20 30 40 50 60 MRWLPGLLLI ASIGFHQSLA DRVLVLGETA AVKDTHSVFL NSVKERGHEL TVRAADDSQL 70 80 90 100 110 120 ALFKHGQLIF DHLFILAPGV QVFGGSLSPS EISKFVDAGG NVLVAAGSNI GDALREIAAE 130 140 150 160 170 180 HGFEFEEAGT SVIDHHNYDQ TLDSGDHTTL VVGKDQLISA ELIVGNSAKL HPVLFKGIGL 190 200 210 220 230 240 VAGKTNNLAL SIVRASGTAY SYDPKAVRAT NPSIAGSRTL LVGGLQSRNN ARIVFTGSSE 250 260 270 280 290 300 LFSNTFFSAK TNSVNPSVQG AQSGNADFAT AITRWVMKES GVLRVKTVNH HKKGETVPPV 310 320 330 340 350 360 EGYFITEDVV YTIEIEELKN GKWVPFQGKD VQLEFVRIDP FVRATLKNSN GRLSVAFKLP 370 380 390 400 410 420 DVLGVFKFLV DYRRVGYTHL YDVQQVSVRP LWHTQYERFI RSAYPYYASS FSMMAGLVLF 430 440 SIVYLYHKDT PVKGAKVLDS EKKKN

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z36753 Genomic DNA. Translation: CAA85337.1.
PIR	T24723.
RefSeq	NP_495655.1. NM_063254.5.
3D structure databases
ProteinModelPortal	P45971.
ModBase	Search...
Protein-protein interaction databases
STRING	6239.T09A5.11.1.
Proteomic databases
PaxDb	P45971.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	T09A5.11.1; T09A5.11.1; T09A5.11. T09A5.11.2; T09A5.11.2; T09A5.11. T09A5.11.3; T09A5.11.3; T09A5.11.
GeneID	174268.
KEGG	cel:CELE_T09A5.11.
UCSC	T09A5.11.2. c. elegans.
Organism-specific databases
CTD	174268.
WormBase	T09A5.11; CE01081; WBGene00011638; ostb-1.
Phylogenomic databases
eggNOG	NOG263094.
GeneTree	ENSGT00390000017294.
HOGENOM	HOG000173744.
InParanoid	P45971.
KO	K12670.
OMA	HHNYDVS.
Enzyme and pathway databases
UniPathway	UPA00378.
Family and domain databases
InterPro	IPR005013. WBP1. [Graphical view]
PANTHER	PTHR10830. PTHR10830. 1 hit.
Pfam	PF03345. DDOST_48kD. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	883303.

Entry information

Entry name

OST48_CAEEL

Accession

Primary (citable) accession number: P45971

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project