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Protein

Probable dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit

Gene

T09A5.11

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains.By similarity

Catalytic activityi

Dolichyl diphosphooligosaccharide + [protein]-L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine.

Pathwayi

GO - Molecular functioni

  1. dolichyl-diphosphooligosaccharide-protein glycotransferase activity Source: InterPro

GO - Biological processi

  1. protein N-linked glycosylation via asparagine Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

ReactomeiREACT_292983. SRP-dependent cotranslational protein targeting to membrane.
REACT_312724. Advanced glycosylation endproduct receptor signaling.
UniPathwayiUPA00378.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit (EC:2.4.99.18)
Short name:
Oligosaccharyl transferase 48 kDa subunit
Gene namesi
ORF Names:T09A5.11
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940 Componenti: Chromosome II

Organism-specific databases

WormBaseiT09A5.11; CE01081; WBGene00011638; ostb-1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini21 – 405385LumenalSequence AnalysisAdd
BLAST
Transmembranei406 – 42621HelicalSequence AnalysisAdd
BLAST
Topological domaini427 – 44519CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence AnalysisAdd
BLAST
Chaini21 – 445425Probable dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunitPRO_0000021954Add
BLAST

Proteomic databases

PaxDbiP45971.

Interactioni

Subunit structurei

Component of the oligosaccharyltransferase (OST) complex.By similarity

Protein-protein interaction databases

STRINGi6239.T09A5.11.1.

Structurei

3D structure databases

ProteinModelPortaliP45971.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DDOST 48 kDa subunit family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG263094.
GeneTreeiENSGT00390000017294.
HOGENOMiHOG000173744.
InParanoidiP45971.
KOiK12670.
OMAiDVSDLGQ.
PhylomeDBiP45971.

Family and domain databases

InterProiIPR005013. WBP1.
[Graphical view]
PANTHERiPTHR10830. PTHR10830. 1 hit.
PfamiPF03345. DDOST_48kD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P45971-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRWLPGLLLI ASIGFHQSLA DRVLVLGETA AVKDTHSVFL NSVKERGHEL
60 70 80 90 100
TVRAADDSQL ALFKHGQLIF DHLFILAPGV QVFGGSLSPS EISKFVDAGG
110 120 130 140 150
NVLVAAGSNI GDALREIAAE HGFEFEEAGT SVIDHHNYDQ TLDSGDHTTL
160 170 180 190 200
VVGKDQLISA ELIVGNSAKL HPVLFKGIGL VAGKTNNLAL SIVRASGTAY
210 220 230 240 250
SYDPKAVRAT NPSIAGSRTL LVGGLQSRNN ARIVFTGSSE LFSNTFFSAK
260 270 280 290 300
TNSVNPSVQG AQSGNADFAT AITRWVMKES GVLRVKTVNH HKKGETVPPV
310 320 330 340 350
EGYFITEDVV YTIEIEELKN GKWVPFQGKD VQLEFVRIDP FVRATLKNSN
360 370 380 390 400
GRLSVAFKLP DVLGVFKFLV DYRRVGYTHL YDVQQVSVRP LWHTQYERFI
410 420 430 440
RSAYPYYASS FSMMAGLVLF SIVYLYHKDT PVKGAKVLDS EKKKN
Length:445
Mass (Da):48,760
Last modified:October 31, 1995 - v1
Checksum:i59A14E6ECF43F0F9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z36753 Genomic DNA. Translation: CAA85337.1.
PIRiT24723.
RefSeqiNP_495655.1. NM_063254.5.
UniGeneiCel.17464.

Genome annotation databases

EnsemblMetazoaiT09A5.11.1; T09A5.11.1; WBGene00011638.
T09A5.11.2; T09A5.11.2; WBGene00011638.
GeneIDi174268.
KEGGicel:CELE_T09A5.11.
UCSCiT09A5.11.2. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z36753 Genomic DNA. Translation: CAA85337.1.
PIRiT24723.
RefSeqiNP_495655.1. NM_063254.5.
UniGeneiCel.17464.

3D structure databases

ProteinModelPortaliP45971.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6239.T09A5.11.1.

Proteomic databases

PaxDbiP45971.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiT09A5.11.1; T09A5.11.1; WBGene00011638.
T09A5.11.2; T09A5.11.2; WBGene00011638.
GeneIDi174268.
KEGGicel:CELE_T09A5.11.
UCSCiT09A5.11.2. c. elegans.

Organism-specific databases

CTDi174268.
WormBaseiT09A5.11; CE01081; WBGene00011638; ostb-1.

Phylogenomic databases

eggNOGiNOG263094.
GeneTreeiENSGT00390000017294.
HOGENOMiHOG000173744.
InParanoidiP45971.
KOiK12670.
OMAiDVSDLGQ.
PhylomeDBiP45971.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiREACT_292983. SRP-dependent cotranslational protein targeting to membrane.
REACT_312724. Advanced glycosylation endproduct receptor signaling.

Miscellaneous databases

NextBioi883303.
PROiP45971.

Family and domain databases

InterProiIPR005013. WBP1.
[Graphical view]
PANTHERiPTHR10830. PTHR10830. 1 hit.
PfamiPF03345. DDOST_48kD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.

Entry informationi

Entry nameiOST48_CAEEL
AccessioniPrimary (citable) accession number: P45971
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 1995
Last sequence update: October 31, 1995
Last modified: March 31, 2015
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.