P45963 (ACR7_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acetylcholine receptor subunit alpha-type acr-7

Gene names

Name:	acr-7
ORF Names:	T09A5.3

Organism

Caenorhabditis elegans

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	538 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane By similarity.
Subunit structure	Forms a homo-oligomeric channel blocked by alpha-bungarotoxin. The structure is probably pentameric By similarity.
Subcellular location	Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein Probable. Cell membrane; Multi-pass membrane protein Probable.
Sequence similarities	Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. [View classification]

Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Cell junction Cell membrane Membrane Postsynaptic cell membrane Synapse
Domain	Signal Transmembrane Transmembrane helix
Molecular function	Ionic channel Ligand-gated ion channel Receptor
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell postsynaptic membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	extracellular ligand-gated ion channel activity Inferred from electronic annotation. Source: InterPro receptor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	Potential
	Chain	28 – 538	511	Acetylcholine receptor subunit alpha-type acr-7		PRO_0000000400
Regions
	Topological domain	28 – 250	223	Extracellular Potential
	Transmembrane	251 – 271	21	Helical; Potential
	Transmembrane	280 – 300	21	Helical; Potential
	Transmembrane	313 – 333	21	Helical; Potential
	Topological domain	334 – 513	180	Cytoplasmic Potential
	Transmembrane	514 – 534	21	Helical; Potential
Amino acid modifications
	Glycosylation	41	1	N-linked (GlcNAc...) Potential
	Glycosylation	101	1	N-linked (GlcNAc...) Ref.3
	Disulfide bond	160 ↔ 174		By similarity
	Disulfide bond	229 ↔ 230		Associated with receptor activation By similarity

Sequences

	Sequence		Length	Mass (Da)
	P45963 [UniParc]. Last modified May 27, 2002. Version 2. Checksum: A33C6514FF64F56C	FASTA	538	62,823
	10 20 30 40 50 60 MMVQSIQIVL PVALFFLIVF NGFTVEGSKK EAQLYRDLLT NYSYLVRPVR NPKKALTVTM 70 80 90 100 110 120 KVFIQQVLLV DAKHQMIEVN AWLKYIWTDF RLRWNPLDYE NITSVRFQGE DQIWQPDILL 130 140 150 160 170 180 YNRYIEDEQE SFDITYKTNA VAYSDGVINW IPPGIFKLSC KMDITLFPFD EQICFMKFGS 190 200 210 220 230 240 WTYHGFALDL RLDVVKGQEP SADLSTYITN GEWHLLSAPA RREEKFYKCC KEPYPTVKFY 250 260 270 280 290 300 LHLRRRTFYY VFNVVLPTLL VSFMSLLAFC LPATDLSEKI GLQTTILLSV CFFLTILSEM 310 320 330 340 350 360 TPTTSEAVPL LGVFFSALTF IVAMSTTFTI LVLNIRYRQI TNHYLSPMFR SIFLECLPWL 370 380 390 400 410 420 MMMKRPDHKF RRGSSYRDSS ADQCVQCAKN AELKSILRGT DNQQQIETNT FYPFPTETLS 430 440 450 460 470 480 LNRKVGDGLF IQRRCQIHEE ARSEKFTHGM LACEKSLREN GSELANILVT ILKMYEVMVS 490 500 510 520 530 QVERIRKRIA LKRKRKDIQD EWKFAAQAVD RFCLIIFTIV FIICCFIFVA IPPIKILD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"An extensive and diverse gene family of nicotinic acetylcholine receptor alpha subunits in Caenorhabditis elegans." Mongan N.P., Baylis H.A., Adcock C., Smith G.R., Sansom M.S.P., Sattelle D.B. Recept. Channels 6:213-228(1998) [PubMed: 10100329] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Bristol N2.
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[3]	"Proteomics reveals N-linked glycoprotein diversity in Caenorhabditis elegans and suggests an atypical translocation mechanism for integral membrane proteins." Kaji H., Kamiie J., Kawakami H., Kido K., Yamauchi Y., Shinkawa T., Taoka M., Takahashi N., Isobe T. Mol. Cell. Proteomics 6:2100-2109(2007) [PubMed: 17761667] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-101, MASS SPECTROMETRY. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF187012 mRNA. Translation: AAG35182.1. Z36753 Genomic DNA. Translation: CAA85338.2.
PIR	T24724.
RefSeq	NP_495647.1. NM_063246.3.
UniGene	Cel.19448.
3D structure databases
ProteinModelPortal	P45963.
SMR	P45963. Positions 28-536.
ModBase	Search...
Proteomic databases
PRIDE	P45963.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	T09A5.3; T09A5.3; T09A5.3.
GeneID	174262.
KEGG	cel:T09A5.3.
NMPDR	fig|6239.3.peg.6386.
UCSC	T09A5.3. c. elegans.
Organism-specific databases
CTD	174262.
WormBase	T09A5.3; CE28078; WBGene00000046; acr-7.
Phylogenomic databases
eggNOG	meNOG06474.
GeneTree	EMGT00050000005191.
HOGENOM	HBG387619.
InParanoid	P45963.
OMA	VINWIPP.
PhylomeDB	P45963.
Family and domain databases
InterPro	IPR006202. Neur_chan_lig-bd. IPR006201. Neur_channel. IPR006029. Neurotrans-gated_channel_TM. IPR018000. Neurotransmitter_ion_chnl_CS. [Graphical view]
Gene3D	G3DSA:2.70.170.10. Neur_chan_lig_bd. 1 hit.
KO	K05312.
PANTHER	PTHR18945. Neur_channel. 1 hit.
Pfam	PF02931. Neur_chan_LBD. 1 hit. PF02932. Neur_chan_memb. 1 hit. [Graphical view]
PRINTS	PR00252. NRIONCHANNEL.
SUPFAM	SSF90112. Neu_channel_TM. 1 hit. SSF63712. Neur_chan_LBD. 1 hit.
TIGRFAMs	TIGR00860. LIC. 1 hit.
PROSITE	PS00236. NEUROTR_ION_CHANNEL. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	883279.

Entry information

Entry name

ACR7_CAEEL

Accession

Primary (citable) accession number: P45963
Secondary accession number(s): Q9GQV0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	May 27, 2002
Last modified:	November 16, 2011
This is version 90 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project