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Protein

Cell division coordinator CpoB

Gene

cpoB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mediates coordination of peptidoglycan synthesis and outer membrane constriction during cell division. Promotes physical and functional coordination of the PBP1B-LpoB and Tol machines, and regulates PBP1B activity in response to Tol energy state.1 Publication

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • cell division Source: EcoCyc
  • protein homooligomerization Source: EcoCyc
  • protein trimerization Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division

Enzyme and pathway databases

BioCyciEcoCyc:EG12854-MONOMER.
ECOL316407:JW0732-MONOMER.

Protein family/group databases

TCDBi2.C.1.2.1. the tonb-exbb-exbd/tola-tolq-tolr (tonb) family of auxiliary proteins for energization of outer membrane receptor (omr)-mediated active transport.

Names & Taxonomyi

Protein namesi
Recommended name:
Cell division coordinator CpoBUniRule annotationCurated
Gene namesi
Name:cpoB1 PublicationUniRule annotation
Synonyms:ybgF
Ordered Locus Names:b0742, JW0732
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12854. ybgF.

Subcellular locationi

GO - Cellular componenti

  • outer membrane-bounded periplasmic space Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

Pathology & Biotechi

Disruption phenotypei

Deletion mutant is sensitive to multiple beta-lactams, including cefsulodin. It leads to defects in outer membrane integrity, envelope defects and elevated cell lysis.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi173Y → D: Decreases self-association. 1 Publication1
Mutagenesisi211P → D: Does not affect oligomerization. 1 Publication1
Mutagenesisi247Y → D: Forms dimers instead of trimers. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 26Sequence analysisAdd BLAST26
ChainiPRO_000001380427 – 263Cell division coordinator CpoBAdd BLAST237

Proteomic databases

EPDiP45955.
PaxDbiP45955.
PRIDEiP45955.

Interactioni

Subunit structurei

Homotrimer (PubMed:20455268, PubMed:20816983). Interacts directly with the central domain of TolA and with PBP1B (PubMed:11994151, PubMed:20816983, PubMed:25951518). Binding to TolA disrupts the homotrimer to form a YbgF/TolA heterodimer with weak affinity (PubMed:20816983). Forms a quaternary complex with PBP1B-LpoB and TolA (PubMed:25951518).4 Publications

Protein-protein interaction databases

BioGridi4260725. 146 interactors.
DIPiDIP-11392N.
IntActiP45955. 3 interactors.
MINTiMINT-1224573.
STRINGi511145.b0742.

Structurei

Secondary structure

1263
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi38 – 99Combined sources62

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2WZ7X-ray2.48A/B/C/D/E/F35-109[»]
2XDJX-ray1.82A/B/C/D/E/F35-109[»]
ProteinModelPortaliP45955.
SMRiP45955.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP45955.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati143 – 176TPR 11 PublicationAdd BLAST34
Repeati180 – 213TPR 21 PublicationAdd BLAST34
Repeati217 – 250TPR 31 PublicationAdd BLAST34

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili44 – 88UniRule annotationAdd BLAST45

Domaini

Contains an N-terminal coiled-coil domain and a C-terminal TPR domain, separated by a flexible linker. The N-terminal domain forms a stable trimer and controls the oligomeric status. The C-terminal domain, which can also trimerize, is involved in interaction with TolA.2 Publications

Sequence similaritiesi

Belongs to the CpoB family.UniRule annotationCurated
Contains 3 TPR repeats.1 Publication

Keywords - Domaini

Coiled coil, Repeat, Signal, TPR repeat

Phylogenomic databases

eggNOGiENOG4105UFD. Bacteria.
COG1729. LUCA.
HOGENOMiHOG000283059.
InParanoidiP45955.
OMAiQYWLGEV.

Family and domain databases

Gene3Di1.25.40.10. 1 hit.
HAMAPiMF_02066. CpoB. 1 hit.
InterProiIPR014162. Tol-Pal_YbgF.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR019734. TPR_repeat.
IPR032519. YbgF_tri.
[Graphical view]
PfamiPF16331. TolA_bind_tri. 1 hit.
PF13174. TPR_6. 1 hit.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 1 hit.
TIGRFAMsiTIGR02795. tol_pal_ybgF. 1 hit.
PROSITEiPS50293. TPR_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P45955-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSNFRHQLL SLSLLVGIAA PWAAFAQAPI SSVGSGSVED RVTQLERISN
60 70 80 90 100
AHSQLLTQLQ QQLSDNQSDI DSLRGQIQEN QYQLNQVVER QKQILLQIDS
110 120 130 140 150
LSSGGAAAQS TSGDQSGAAA STTPTADAGT ANAGAPVKSG NANTDYNAAI
160 170 180 190 200
ALVQDKSRQD DAMVAFQNFI KNYPDSTYLP NANYWLGQLN YNKGKKDDAA
210 220 230 240 250
YYFASVVKNY PKSPKAADAM FKVGVIMQDK GDTAKAKAVY QQVISKYPGT
260
DGAKQAQKRL NAM
Length:263
Mass (Da):28,231
Last modified:November 1, 1997 - v2
Checksum:iEE9845C0A8E803A2
GO

Sequence cautioni

The sequence X65796 differs from that shown. Reason: Frameshift at positions 24 and 41.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti224G → A in X65796 (PubMed:1574003).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X65796 Genomic DNA. No translation available.
U00096 Genomic DNA. Translation: AAC73836.1.
AP009048 Genomic DNA. Translation: BAA35408.1.
PIRiE64810.
RefSeqiNP_415270.1. NC_000913.3.
WP_000097571.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73836; AAC73836; b0742.
BAA35408; BAA35408; BAA35408.
GeneIDi947227.
KEGGiecj:JW0732.
eco:b0742.
PATRICi32116683. VBIEscCol129921_0774.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X65796 Genomic DNA. No translation available.
U00096 Genomic DNA. Translation: AAC73836.1.
AP009048 Genomic DNA. Translation: BAA35408.1.
PIRiE64810.
RefSeqiNP_415270.1. NC_000913.3.
WP_000097571.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2WZ7X-ray2.48A/B/C/D/E/F35-109[»]
2XDJX-ray1.82A/B/C/D/E/F35-109[»]
ProteinModelPortaliP45955.
SMRiP45955.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260725. 146 interactors.
DIPiDIP-11392N.
IntActiP45955. 3 interactors.
MINTiMINT-1224573.
STRINGi511145.b0742.

Protein family/group databases

TCDBi2.C.1.2.1. the tonb-exbb-exbd/tola-tolq-tolr (tonb) family of auxiliary proteins for energization of outer membrane receptor (omr)-mediated active transport.

Proteomic databases

EPDiP45955.
PaxDbiP45955.
PRIDEiP45955.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73836; AAC73836; b0742.
BAA35408; BAA35408; BAA35408.
GeneIDi947227.
KEGGiecj:JW0732.
eco:b0742.
PATRICi32116683. VBIEscCol129921_0774.

Organism-specific databases

EchoBASEiEB2698.
EcoGeneiEG12854. ybgF.

Phylogenomic databases

eggNOGiENOG4105UFD. Bacteria.
COG1729. LUCA.
HOGENOMiHOG000283059.
InParanoidiP45955.
OMAiQYWLGEV.

Enzyme and pathway databases

BioCyciEcoCyc:EG12854-MONOMER.
ECOL316407:JW0732-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP45955.
PROiP45955.

Family and domain databases

Gene3Di1.25.40.10. 1 hit.
HAMAPiMF_02066. CpoB. 1 hit.
InterProiIPR014162. Tol-Pal_YbgF.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR019734. TPR_repeat.
IPR032519. YbgF_tri.
[Graphical view]
PfamiPF16331. TolA_bind_tri. 1 hit.
PF13174. TPR_6. 1 hit.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 1 hit.
TIGRFAMsiTIGR02795. tol_pal_ybgF. 1 hit.
PROSITEiPS50293. TPR_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCPOB_ECOLI
AccessioniPrimary (citable) accession number: P45955
Secondary accession number(s): P75756
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.