Reviewed, UniProtKB/Swiss-Prot P45953 (ACADV_RAT)

Names and origin

Protein names

Recommended name:
    Very long-chain specific acyl-CoA dehydrogenase, mitochondrial
      Short name=VLCAD
    EC=1.3.99.-

Gene names

Name:	Acadvl
Synonyms:	Vlcad

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	655 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Active toward esters of long-chain and very long chain fatty acids such as palmitoyl-CoA, mysritoyl-CoA and stearoyl-CoA. Can accomodate substrate acyl chain lengths as long as 24 carbons, but shows little activity for substrates of less than 12 carbons.
Catalytic activity	Acyl-CoA + ETF = 2,3-dehydroacyl-CoA + reduced ETF.
Cofactor	FAD.
Pathway	Lipid metabolism; mitochondrial fatty acid beta-oxidation.
Subunit structure	Homodimer.
Subcellular location	Mitochondrion inner membrane.
Miscellaneous	A number of straight-chain acyl-CoA dehydrogenases of different substrate specificities are present in mammalian tissues.
Sequence similarities	Belongs to the acyl-CoA dehydrogenase family.

Ontologies

Keywords
Biological process	Fatty acid metabolism Lipid metabolism
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
PTM	Acetylation Isopeptide bond Ubl conjugation
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	fatty acid beta-oxidation Ref.1 Inferred from direct assay. Source: RGD oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro long-chain-acyl-CoA dehydrogenase activity Ref.1 Inferred from direct assay. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 40	40	Mitochondrion
	Chain	41 – 655	615	Very long-chain specific acyl-CoA dehydrogenase, mitochondrial		PRO_0000000518
Regions
	Region	41 – 482	442	Catalytic
	Region	483 – 516	34	Membrane-anchoring Probable
Sites
	Active site	462	1	Proton acceptor By similarity
Amino acid modifications
	Modified residue	239	1	N6-acetyllysine By similarity
	Modified residue	276	1	N6-acetyllysine By similarity
	Cross-link	331		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P45953-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: E808EDEB0E4595D7	FASTA	655	70,749
	10 20 30 40 50 60 MQSARMTPSV GRQLLRLGAR SSRSAALQGQ PRPTSAQRLY ASEATQAVLE KPETLSSDAS 70 80 90 100 110 120 TREKPARAES KSFAVGMFKG QLTTDQVFPY PSVLNEGQTQ FLKELVGPVA RFFEEVNDPA 130 140 150 160 170 180 KNDSLEKVEE DTLQGLKELG AFGLQVPSEL GGLGLSNTQY ARLAEIVGMH DLGVSVTLGA 190 200 210 220 230 240 HQSIGFKGIL LYGTKAQKEK YLPRVASGQA LAAFCLTEPS SGSDVASIRS SAVPSPCGKY 250 260 270 280 290 300 YTLNGSKIWI SNGGLADIFT VFAKTPIKDA ATGAVKEKIT AFVVERSFGG VTHGLPEKKM 310 320 330 340 350 360 GIKASNTSEV YFDGVKVPAE NVLGEVGDGF KVAVNILNNG RFGMAATLAG TMKAIIAKAV 370 380 390 400 410 420 DHATNRTQFG DKIHNFGVIQ EKLARMAILQ YVTESMAYML SANMDQGFKD FQIEAAISKI 430 440 450 460 470 480 FGSEAAWKVT DECIQIMGGM GFMKEPGVER VLRDIRIFRI FEGTNDILRL FVALQGCMDK 490 500 510 520 530 540 GKELTGLGNA LKNPLGNVGL LIGEASKQLR RRTGIGSGLS LSGIVHPELS RSGELAVQAL 550 560 570 580 590 600 EQFATVVEAK LMKHKKGIVN EQFLLQRLAD GAIDLYAMVV VLSRASRSLS EGYPTAQHEK 610 620 630 640 650 MLCDSWCIEA ATRIRENMAS LQSNPQQQEL FRNFRSISKA MVENGGLVTS NPLRV

References

[1]

"Rat very-long-chain acyl-CoA dehydrogenase, a novel mitochondrial acyl-CoA dehydrogenase gene product, is a rate-limiting enzyme in long-chain fatty acid beta-oxidation system. cDNA and deduced amino acid sequence and distinct specificities of the cDNA-expressed protein." Aoyama T., Ueno I., Kamijo T., Hashimoto T. J. Biol. Chem. 269:19088-19094(1994) [PubMed: 8034667] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Strain: Wistar. Tissue: Liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D30647 mRNA. Translation: BAA06331.1.
IPI	IPI00213057.
PIR	A54872.
RefSeq	NP_037023.1.
UniGene	Rn.33319
3D structure databases
HSSP	HSSP built from PDB template 1BUC based on UniProtKB Q06319.
SMR	P45953. Positions 69-653.
ModBase	Search...
Proteomic databases
PRIDE	P45953.
Genome annotation databases
Ensembl	ENSRNOG00000018114. Rattus norvegicus. [Contig view]
GeneID	25363.
KEGG	rno:25363.
Organism-specific databases
RGD	2014. Acadvl.
Phylogenomic databases
HOVERGEN	P45953.
Gene expression databases
ArrayExpress	P45953.
GermOnline	ENSRNOG00000018114. Rattus norvegicus.
Family and domain databases
InterPro	IPR006089. Acyl-CoA_DH_CS. IPR006092. Acyl-CoA_DH_N. IPR006090. Acyl-CoA_Oxase/DH_1. IPR006091. Acyl-CoA_Oxase/DH_M. IPR013786. AcylCoA_DH/ox_N. IPR013764. AcylCoA_oxidase/DH_1/2_C. [Graphical view]
Gene3D	G3DSA:2.40.110.10. Acyl_CoA_DH/ox_M. 1 hit. G3DSA:1.10.540.10. AcylCoA_DH/ox_N. 1 hit. G3DSA:1.20.140.10. AcylCoA_DH_1/2_C. 1 hit.
Pfam	PF00441. Acyl-CoA_dh_1. 1 hit. PF02770. Acyl-CoA_dh_M. 1 hit. PF02771. Acyl-CoA_dh_N. 1 hit. [Graphical view]
PROSITE	PS00072. ACYL_COA_DH_1. 1 hit. PS00073. ACYL_COA_DH_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	606351.

Entry information

Entry name

ACADV_RAT

Accession

Primary (citable) accession number: P45953

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	June 16, 2009
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)