Reviewed,
UniProtKB/Swiss-Prot P45952 (ACADM_MOUSE)
Last modified
November 25, 2008.
Version 82.
History...
Clusters with 100%,
90%,
50% identity |
Documents (4) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Medium-chain specific acyl-CoA dehydrogenase, mitochondrial Short name=MCAD EC=1.3.99.3 | ||
| Gene names |
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| Organism | Mus musculus (Mouse) | ||
| Taxonomic identifier | 10090 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus |
Protein attributes
| Sequence length | 421 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | This enzyme is specific for acyl chain lengths of 4 to 16. |
| Catalytic activity | Acyl-CoA + acceptor = 2,3-dehydroacyl-CoA + reduced acceptor. |
| Cofactor | FAD By similarity. |
| Pathway | |
| Subunit structure | Homotetramer By similarity. |
| Subcellular location | Mitochondrion matrixBy similarity. |
| Miscellaneous | A number of straight-chain acyl-CoA dehydrogenases of different substrate specificities are present in mammalian tissues. |
| Sequence similarities | Belongs to the acyl-CoA dehydrogenase family. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Transit peptide | 1 – 25 | 25 | Mitochondrion By similarity | ||||||
| Chain | 26 – 421 | 396 | Medium-chain specific acyl-CoA dehydrogenase, mitochondrial | PRO_0000000504 | |||||
Regions | |||||||||
| Nucleotide binding | 158 – 167 | 10 | FAD By similarity | ||||||
| Nucleotide binding | 191 – 193 | 3 | FAD By similarity | ||||||
| Nucleotide binding | 306 – 308 | 3 | FAD By similarity | ||||||
| Nucleotide binding | 316 – 317 | 2 | FAD By similarity | ||||||
| Nucleotide binding | 374 – 378 | 5 | FAD By similarity | ||||||
| Nucleotide binding | 403 – 405 | 3 | FAD By similarity | ||||||
| Region | 278 – 281 | 4 | Substrate binding By similarity | ||||||
Sites | |||||||||
| Active site | 401 | 1 | Proton acceptor By similarity | ||||||
| Binding site | 167 | 1 | Substrate; via carbonyl oxygen By similarity | ||||||
| Binding site | 402 | 1 | Substrate; via amide nitrogen By similarity | ||||||
Experimental info | |||||||||
| Sequence conflict | 135 | 1 | L → I Ref.2 | ||||||
| Sequence conflict | 184 | 1 | V → A Ref.2 | ||||||
Sequences
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References
| [1] | "Molecular cloning and characterization of the mouse medium-chain acyl-CoA dehydrogenase cDNA." Tolwani R.J., Farmer S.C., Wood P.A. Genomics 23:247-249(1994) [PubMed: 7829081] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c. Tissue: Liver. |
| [2] | "Molecular studies of mouse medium and long-chain acyl-CoA dehydrogenase genes for site-directed mutagenesis of embryonic stem cells." Wood P.A., Farmer S.C., Tolwani R.J., Warren J.R., Steinkampf M.P., Johnson L.W., Mountz J.D., Kelly D.P. Prog. Clin. Biol. Res. 375:151-160(1992) [PubMed: 1438358] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 134-193. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| U07159 mRNA. Translation: AAA76733.1. S48761 Genomic DNA. No translation available. S48759 Genomic DNA. No translation available. | |
| PIR | A55724. |
| RefSeq | NP_031408.1. |
| UniGene | Mm.10530 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1EGD based on UniProtKB P11310. |
| SMR | P45952. Positions 35-421. |
| ModBase | Search... |
PTM databases | |
| PhosphoSite | P45952. |
2-D gel databases | |
| SWISS-2DPAGE | P45952. |
Genome annotation databases | |
| Ensembl | ENSMUSG00000062908. Mus musculus. [Contig view] |
| GeneID | 11364. |
| KEGG | mmu:11364. |
| NMPDR | fig|10090.3.peg.9178. |
Organism-specific databases | |
| MGI | MGI:87867. Acadm. |
Phylogenomic databases | |
| HOGENOM | P45952. |
| HOVERGEN | P45952. |
Gene expression databases | |
| ArrayExpress | P45952. |
| CleanEx | MM_ACADM. |
| GermOnline | ENSMUSG00000062908. Mus musculus. |
Family and domain databases | |
| InterPro | IPR006091. Acyl-CoA_DHase/Oxase_M. IPR006089. Acyl-CoA_DHase_CS. IPR006092. Acyl-CoA_DHase_N. IPR006090. Acyl-CoA_Oxase/DHase_1. IPR013786. AcylCoA_DH/ox_N. IPR013764. AcylCoA_oxidase/DH_1/2_C. [Graphical view] |
| Gene3D | G3DSA:2.40.110.10. Acyl_CoA_DH/ox_M. 1 hit. G3DSA:1.10.540.10. AcylCoA_DH/ox_N. 1 hit. G3DSA:1.20.140.10. AcylCoA_DH_1/2_C. 1 hit. |
| Pfam | PF00441. Acyl-CoA_dh_1. 1 hit. PF02770. Acyl-CoA_dh_M. 1 hit. PF02771. Acyl-CoA_dh_N. 1 hit. [Graphical view] |
| PROSITE | PS00072. ACYL_COA_DH_1. 1 hit. PS00073. ACYL_COA_DH_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 278640. |
| SOURCE | Search... |
Entry information
| Entry name | ACADM_MOUSE | ||||||||
| Accession | Primary (citable) accession number: P45952 Secondary accession number(s): Q64235 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| UniProtKB secondary accession numbers Index of UniProtKB secondary accession numbers |
| SIMILARITY comments Index of protein domains and families |

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