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P45951 (ARP_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 125. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Apurinic endonuclease-redox protein
Alternative name(s):
DNA-(apurinic or apyrimidinic site) lyase
EC=4.2.99.18
Gene names
Name:ARP
Synonyms:REF
Ordered Locus Names:At2g41460
ORF Names:T26J13.5
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length536 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Repairs oxidative DNA damages, seems also to act as a redox factor. Is multifunctional and may be involved both in DNA repair and in the regulation of transcription.

Catalytic activity

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.

Cofactor

Magnesium. Can also utilize manganese. Probably binds two magnesium or manganese ions per subunit By similarity.

Subcellular location

Nucleus.

Tissue specificity

Expressed in the siliques, flowers, and stems. A high level expression is seen in the leaves.

Sequence similarities

Belongs to the DNA repair enzymes AP/ExoA family.

Contains 1 SAP domain.

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: P45951-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 536536Apurinic endonuclease-redox protein
PRO_0000200018

Regions

Domain97 – 13135SAP
Region1 – 278278Highly charged; increases the affinity of ARP for DNA
Region279 – 536258AP endonuclease

Sites

Active site3891 By similarity
Active site4291Proton donor/acceptor By similarity
Metal binding3131Magnesium 1 By similarity
Metal binding4291Magnesium 2 By similarity
Metal binding4311Magnesium 2 By similarity
Metal binding5261Magnesium 1 By similarity
Site4311Transition state stabilizer By similarity
Site5011Important for catalytic activity By similarity
Site5271Interaction with DNA substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 30, 2000. Version 2.
Checksum: 5C1FC17EA991D27B

FASTA53660,260
        10         20         30         40         50         60 
MNNVLQFGLQ SSAIYVAKFL VVPLRSLRVG SSFVGVGVGT RSFNKRLMSN ATAFSINNSK 

        70         80         90        100        110        120 
RKELKIPGAA IDQNCHQMGS DTDRDEMGTL QDDRKEIEAM TVQELRSTLR KLGVPVKGRK 

       130        140        150        160        170        180 
QELISTLRLH MDSNLPDQKE TSSSTRSDSV TIKRKISNRE EPTEDECTNS EAYDIEHGEK 

       190        200        210        220        230        240 
RVKQSTEKNL KAKVSAKAIA KEQKSLMRTG KQQIQSKEET SSTISSELLK TEEIISSPSQ 

       250        260        270        280        290        300 
SEPWTVLAHK KPQKDWKAYN PKTMRPPPLP EGTKCVKVMT WNVNGLRGLL KFESFSALQL 

       310        320        330        340        350        360 
AQRENFDILC LQETKLQVKD VEEIKKTLID GYDHSFWSCS VSKLGYSGTA IISRIKPLSV 

       370        380        390        400        410        420 
RYGTGLSGHD TEGRIVTAEF DSFYLINTYV PNSGDGLKRL SYRIEEWDRT LSNHIKELEK 

       430        440        450        460        470        480 
SKPVVLTGDL NCAHEEIDIF NPAGNKRSAG FTIEERQSFG ANLLDKGFVD TFRKQHPGVV 

       490        500        510        520        530 
GYTYWGYRHG GRKTNKGWRL DYFLVSQSIA ANVHDSYILP DINGSDHCPI GLILKL 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"The Arabidopsis thaliana apurinic endonuclease Arp reduces human transcription factors Fos and Jun."
Babiychuk E., Kushnir S., van Montagu M., Inze D.
Proc. Natl. Acad. Sci. U.S.A. 91:3299-3303(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 10-536.
Strain: cv. Columbia.
Tissue: Callus.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC004625 Genomic DNA. Translation: AAC23731.1.
CP002685 Genomic DNA. Translation: AEC09984.1.
X76912 mRNA. Translation: CAA54234.1.
PIRT02441.
RefSeqNP_181677.1. NM_129709.4. [P45951-1]
UniGeneAt.25567.

3D structure databases

ProteinModelPortalP45951.
SMRP45951. Positions 241-536.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PaxDbP45951.
PRIDEP45951.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G41460.1; AT2G41460.1; AT2G41460. [P45951-1]
GeneID818744.
KEGGath:AT2G41460.

Organism-specific databases

TAIRAT2G41460.

Phylogenomic databases

eggNOGCOG0708.
InParanoidP45951.
KOK01142.
OMAQRENFDI.
PhylomeDBP45951.

Enzyme and pathway databases

BioCycARA:AT2G41460-MONOMER.
ARA:GQT-950-MONOMER.

Gene expression databases

ArrayExpressP45951.
GenevestigatorP45951.

Family and domain databases

Gene3D1.10.720.30. 1 hit.
3.60.10.10. 1 hit.
InterProIPR004808. AP_endonuc_1.
IPR020847. AP_endonuclease_F1_BS.
IPR020848. AP_endonuclease_F1_CS.
IPR005135. Endo/exonuclease/phosphatase.
IPR003034. SAP_dom.
[Graphical view]
PANTHERPTHR22748. PTHR22748. 1 hit.
PfamPF03372. Exo_endo_phos. 1 hit.
PF02037. SAP. 1 hit.
[Graphical view]
SMARTSM00513. SAP. 1 hit.
[Graphical view]
SUPFAMSSF56219. SSF56219. 1 hit.
TIGRFAMsTIGR00633. xth. 1 hit.
PROSITEPS00726. AP_NUCLEASE_F1_1. 1 hit.
PS00727. AP_NUCLEASE_F1_2. 1 hit.
PS00728. AP_NUCLEASE_F1_3. 1 hit.
PS51435. AP_NUCLEASE_F1_4. 1 hit.
PS50800. SAP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP45951.

Entry information

Entry nameARP_ARATH
AccessionPrimary (citable) accession number: P45951
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: May 30, 2000
Last modified: June 11, 2014
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names