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P45951

- ARP_ARATH

UniProt

P45951 - ARP_ARATH

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Protein

Apurinic endonuclease-redox protein

Gene

ARP

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Repairs oxidative DNA damages, seems also to act as a redox factor. Is multifunctional and may be involved both in DNA repair and in the regulation of transcription.

Catalytic activityi

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.PROSITE-ProRule annotation

Cofactori

Magnesium. Can also utilize manganese. Probably binds two magnesium or manganese ions per subunit By similarity.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi313 – 3131Magnesium 1By similarity
Active sitei389 – 3891By similarity
Active sitei429 – 4291Proton donor/acceptorBy similarity
Metal bindingi429 – 4291Magnesium 2By similarity
Metal bindingi431 – 4311Magnesium 2By similarity
Sitei431 – 4311Transition state stabilizerBy similarity
Sitei501 – 5011Important for catalytic activityBy similarity
Metal bindingi526 – 5261Magnesium 1By similarity
Sitei527 – 5271Interaction with DNA substrateBy similarity

GO - Molecular functioni

  1. DNA-(apurinic or apyrimidinic site) lyase activity Source: TAIR
  2. DNA binding Source: InterPro
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. DNA catabolic process, endonucleolytic Source: GOC
  2. DNA repair Source: UniProtKB-KW
  3. positive regulation of transcription, DNA-templated Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT2G41460-MONOMER.
ARA:GQT-950-MONOMER.
ReactomeiREACT_77052. Displacement of DNA glycosylase by APE1.
REACT_90136. Base-free sugar-phosphate removal via the single-nucleotide replacement pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Apurinic endonuclease-redox protein
Alternative name(s):
DNA-(apurinic or apyrimidinic site) lyase (EC:4.2.99.18)
Gene namesi
Name:ARP
Synonyms:REF
Ordered Locus Names:At2g41460
ORF Names:T26J13.5
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G41460.

Subcellular locationi

GO - Cellular componenti

  1. chloroplast nucleoid Source: TAIR
  2. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 536536Apurinic endonuclease-redox proteinPRO_0000200018Add
BLAST

Proteomic databases

PaxDbiP45951.
PRIDEiP45951.

Expressioni

Tissue specificityi

Expressed in the siliques, flowers, and stems. A high level expression is seen in the leaves.

Gene expression databases

ExpressionAtlasiP45951. baseline and differential.
GenevestigatoriP45951.

Structurei

3D structure databases

ProteinModelPortaliP45951.
SMRiP45951. Positions 241-536.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini97 – 13135SAPPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 278278Highly charged; increases the affinity of ARP for DNAAdd
BLAST
Regioni279 – 536258AP endonucleaseAdd
BLAST

Sequence similaritiesi

Belongs to the DNA repair enzymes AP/ExoA family.Curated
Contains 1 SAP domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0708.
InParanoidiP45951.
KOiK01142.
OMAiQRENFDI.
PhylomeDBiP45951.

Family and domain databases

Gene3Di1.10.720.30. 1 hit.
3.60.10.10. 1 hit.
InterProiIPR004808. AP_endonuc_1.
IPR020847. AP_endonuclease_F1_BS.
IPR020848. AP_endonuclease_F1_CS.
IPR005135. Endo/exonuclease/phosphatase.
IPR003034. SAP_dom.
[Graphical view]
PANTHERiPTHR22748. PTHR22748. 1 hit.
PfamiPF03372. Exo_endo_phos. 1 hit.
PF02037. SAP. 1 hit.
[Graphical view]
SMARTiSM00513. SAP. 1 hit.
[Graphical view]
SUPFAMiSSF56219. SSF56219. 1 hit.
TIGRFAMsiTIGR00633. xth. 1 hit.
PROSITEiPS00726. AP_NUCLEASE_F1_1. 1 hit.
PS00727. AP_NUCLEASE_F1_2. 1 hit.
PS00728. AP_NUCLEASE_F1_3. 1 hit.
PS51435. AP_NUCLEASE_F1_4. 1 hit.
PS50800. SAP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. Align

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: P45951-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNNVLQFGLQ SSAIYVAKFL VVPLRSLRVG SSFVGVGVGT RSFNKRLMSN
60 70 80 90 100
ATAFSINNSK RKELKIPGAA IDQNCHQMGS DTDRDEMGTL QDDRKEIEAM
110 120 130 140 150
TVQELRSTLR KLGVPVKGRK QELISTLRLH MDSNLPDQKE TSSSTRSDSV
160 170 180 190 200
TIKRKISNRE EPTEDECTNS EAYDIEHGEK RVKQSTEKNL KAKVSAKAIA
210 220 230 240 250
KEQKSLMRTG KQQIQSKEET SSTISSELLK TEEIISSPSQ SEPWTVLAHK
260 270 280 290 300
KPQKDWKAYN PKTMRPPPLP EGTKCVKVMT WNVNGLRGLL KFESFSALQL
310 320 330 340 350
AQRENFDILC LQETKLQVKD VEEIKKTLID GYDHSFWSCS VSKLGYSGTA
360 370 380 390 400
IISRIKPLSV RYGTGLSGHD TEGRIVTAEF DSFYLINTYV PNSGDGLKRL
410 420 430 440 450
SYRIEEWDRT LSNHIKELEK SKPVVLTGDL NCAHEEIDIF NPAGNKRSAG
460 470 480 490 500
FTIEERQSFG ANLLDKGFVD TFRKQHPGVV GYTYWGYRHG GRKTNKGWRL
510 520 530
DYFLVSQSIA ANVHDSYILP DINGSDHCPI GLILKL
Length:536
Mass (Da):60,260
Last modified:May 30, 2000 - v2
Checksum:i5C1FC17EA991D27B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC004625 Genomic DNA. Translation: AAC23731.1.
CP002685 Genomic DNA. Translation: AEC09984.1.
X76912 mRNA. Translation: CAA54234.1.
PIRiT02441.
RefSeqiNP_181677.1. NM_129709.4. [P45951-1]
UniGeneiAt.25567.

Genome annotation databases

EnsemblPlantsiAT2G41460.1; AT2G41460.1; AT2G41460. [P45951-1]
GeneIDi818744.
KEGGiath:AT2G41460.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC004625 Genomic DNA. Translation: AAC23731.1 .
CP002685 Genomic DNA. Translation: AEC09984.1 .
X76912 mRNA. Translation: CAA54234.1 .
PIRi T02441.
RefSeqi NP_181677.1. NM_129709.4. [P45951-1 ]
UniGenei At.25567.

3D structure databases

ProteinModelPortali P45951.
SMRi P45951. Positions 241-536.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PaxDbi P45951.
PRIDEi P45951.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G41460.1 ; AT2G41460.1 ; AT2G41460 . [P45951-1 ]
GeneIDi 818744.
KEGGi ath:AT2G41460.

Organism-specific databases

TAIRi AT2G41460.

Phylogenomic databases

eggNOGi COG0708.
InParanoidi P45951.
KOi K01142.
OMAi QRENFDI.
PhylomeDBi P45951.

Enzyme and pathway databases

BioCyci ARA:AT2G41460-MONOMER.
ARA:GQT-950-MONOMER.
Reactomei REACT_77052. Displacement of DNA glycosylase by APE1.
REACT_90136. Base-free sugar-phosphate removal via the single-nucleotide replacement pathway.

Miscellaneous databases

PROi P45951.

Gene expression databases

ExpressionAtlasi P45951. baseline and differential.
Genevestigatori P45951.

Family and domain databases

Gene3Di 1.10.720.30. 1 hit.
3.60.10.10. 1 hit.
InterProi IPR004808. AP_endonuc_1.
IPR020847. AP_endonuclease_F1_BS.
IPR020848. AP_endonuclease_F1_CS.
IPR005135. Endo/exonuclease/phosphatase.
IPR003034. SAP_dom.
[Graphical view ]
PANTHERi PTHR22748. PTHR22748. 1 hit.
Pfami PF03372. Exo_endo_phos. 1 hit.
PF02037. SAP. 1 hit.
[Graphical view ]
SMARTi SM00513. SAP. 1 hit.
[Graphical view ]
SUPFAMi SSF56219. SSF56219. 1 hit.
TIGRFAMsi TIGR00633. xth. 1 hit.
PROSITEi PS00726. AP_NUCLEASE_F1_1. 1 hit.
PS00727. AP_NUCLEASE_F1_2. 1 hit.
PS00728. AP_NUCLEASE_F1_3. 1 hit.
PS51435. AP_NUCLEASE_F1_4. 1 hit.
PS50800. SAP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "The Arabidopsis thaliana apurinic endonuclease Arp reduces human transcription factors Fos and Jun."
    Babiychuk E., Kushnir S., van Montagu M., Inze D.
    Proc. Natl. Acad. Sci. U.S.A. 91:3299-3303(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 10-536.
    Strain: cv. Columbia.
    Tissue: Callus.

Entry informationi

Entry nameiARP_ARATH
AccessioniPrimary (citable) accession number: P45951
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: May 30, 2000
Last modified: October 29, 2014
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3