Reviewed, UniProtKB/Swiss-Prot P45951 (ARP_ARATH)

Names and origin

Protein names

Recommended name:
    Apurinic endonuclease-redox protein
Alternative name(s):
    DNA-(apurinic or apyrimidinic site) lyase
    EC=4.2.99.18

Gene names

Name:	ARP
Synonyms:	REF
Ordered Locus Names:	At2g41460
ORF Names:	T26J13.5

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	536 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Repairs oxidative DNA damages, seems also to act as a redox factor. Is multifunctional and may be involved both in DNA repair and in the regulation of transcription.
Catalytic activity	The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.
Subcellular location	Nucleus.
Tissue specificity	Expressed in the siliques, flowers, and stems. A high level expression is seen in the leaves.
Sequence similarities	Belongs to the DNA repair enzymes AP/exoA family. Contains 1 SAP domain.

Ontologies

Keywords
Biological process	DNA damage DNA repair
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Ligand	Magnesium Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of transcription Ref.2 Traceable author statement. Source: TAIR
Cellular component	chloroplast nucleoid Inferred from direct assay. Source: TAIR nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-(apurinic or apyrimidinic site) lyase activity Ref.2 Inferred from direct assay. Source: TAIR endonuclease activity Inferred from electronic annotation. Source: InterPro magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: P45951-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 536	536	Apurinic endonuclease-redox protein		PRO_0000200018
Regions
	Domain	97 – 131	35	SAP
	Region	1 – 278	278	Highly charged; increases the affinity of ARP for DNA
	Region	279 – 536	258	AP endonuclease
Sites
	Active site	527	1	Proton acceptor By similarity
	Metal binding	313	1	Magnesium or manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 30, 2000. Version 2. Checksum: 5C1FC17EA991D27B	FASTA	536	60,260
	10 20 30 40 50 60 MNNVLQFGLQ SSAIYVAKFL VVPLRSLRVG SSFVGVGVGT RSFNKRLMSN ATAFSINNSK 70 80 90 100 110 120 RKELKIPGAA IDQNCHQMGS DTDRDEMGTL QDDRKEIEAM TVQELRSTLR KLGVPVKGRK 130 140 150 160 170 180 QELISTLRLH MDSNLPDQKE TSSSTRSDSV TIKRKISNRE EPTEDECTNS EAYDIEHGEK 190 200 210 220 230 240 RVKQSTEKNL KAKVSAKAIA KEQKSLMRTG KQQIQSKEET SSTISSELLK TEEIISSPSQ 250 260 270 280 290 300 SEPWTVLAHK KPQKDWKAYN PKTMRPPPLP EGTKCVKVMT WNVNGLRGLL KFESFSALQL 310 320 330 340 350 360 AQRENFDILC LQETKLQVKD VEEIKKTLID GYDHSFWSCS VSKLGYSGTA IISRIKPLSV 370 380 390 400 410 420 RYGTGLSGHD TEGRIVTAEF DSFYLINTYV PNSGDGLKRL SYRIEEWDRT LSNHIKELEK 430 440 450 460 470 480 SKPVVLTGDL NCAHEEIDIF NPAGNKRSAG FTIEERQSFG ANLLDKGFVD TFRKQHPGVV 490 500 510 520 530 GYTYWGYRHG GRKTNKGWRL DYFLVSQSIA ANVHDSYILP DINGSDHCPI GLILKL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"The Arabidopsis thaliana apurinic endonuclease Arp reduces human transcription factors Fos and Jun." Babiychuk E., Kushnir S., van Montagu M., Inze D. Proc. Natl. Acad. Sci. U.S.A. 91:3299-3303(1994) [PubMed: 7512729] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 10-536. Strain: cv. Columbia. Tissue: Callus.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC004625 Genomic DNA. Translation: AAC23731.1. X76912 mRNA. Translation: CAA54234.1.
IPI	IPI00529731.
PIR	T02441.
RefSeq	NP_181677.1.
UniGene	At.25567
3D structure databases
SMR	P45951. Positions 240-536.
ModBase	Search...
Protein-protein interaction databases
STRING	P45951.
Proteomic databases
PRIDE	P45951.
Genome annotation databases
GeneID	818744.
NMPDR	fig|3702.1.peg.11284.
Organism-specific databases
TAIR	At2g41460.
Phylogenomic databases
InParanoid	P45951.
OMA	IFNPAGN.
PhylomeDB	P45951.
Enzyme and pathway databases
BRENDA	4.2.99.18. 302.
Gene expression databases
ArrayExpress	P45951.
Genevestigator	P45951.
GermOnline	AT2G41460. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR000097. AP_endonuclease_F1. IPR020847. AP_endonuclease_F1_BS. IPR020848. AP_endonuclease_F1_CS. IPR005135. Endo/exonuclease/phosphatase. IPR004808. exoDNase_III. IPR003034. SAP_DNA_bd. [Graphical view]
PANTHER	PTHR22748. ExoIII_xth. 1 hit.
Pfam	PF03372. Exo_endo_phos. 1 hit. PF02037. SAP. 1 hit. [Graphical view]
SMART	SM00513. SAP. 1 hit. [Graphical view]
TIGRFAMs	TIGR00195. exoDNase_III. 1 hit. TIGR00633. xth. 1 hit.
PROSITE	PS00726. AP_NUCLEASE_F1_1. 1 hit. PS00727. AP_NUCLEASE_F1_2. 1 hit. PS00728. AP_NUCLEASE_F1_3. 1 hit. PS51435. AP_NUCLEASE_F1_4. 1 hit. PS50800. SAP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ARP_ARATH

Accession

Primary (citable) accession number: P45951

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	May 30, 2000
Last modified:	February 9, 2010
This is version 86 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)