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P45951

- ARP_ARATH

UniProt

P45951 - ARP_ARATH

Protein

Apurinic endonuclease-redox protein

Gene

ARP

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 127 (01 Oct 2014)
      Sequence version 2 (30 May 2000)
      Previous versions | rss
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    Functioni

    Repairs oxidative DNA damages, seems also to act as a redox factor. Is multifunctional and may be involved both in DNA repair and in the regulation of transcription.

    Catalytic activityi

    The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.PROSITE-ProRule annotation

    Cofactori

    Magnesium. Can also utilize manganese. Probably binds two magnesium or manganese ions per subunit By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi313 – 3131Magnesium 1By similarity
    Active sitei389 – 3891By similarity
    Active sitei429 – 4291Proton donor/acceptorBy similarity
    Metal bindingi429 – 4291Magnesium 2By similarity
    Metal bindingi431 – 4311Magnesium 2By similarity
    Sitei431 – 4311Transition state stabilizerBy similarity
    Sitei501 – 5011Important for catalytic activityBy similarity
    Metal bindingi526 – 5261Magnesium 1By similarity
    Sitei527 – 5271Interaction with DNA substrateBy similarity

    GO - Molecular functioni

    1. DNA-(apurinic or apyrimidinic site) lyase activity Source: TAIR
    2. DNA binding Source: InterPro
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. DNA catabolic process, endonucleolytic Source: GOC
    2. DNA repair Source: UniProtKB-KW
    3. positive regulation of transcription, DNA-templated Source: TAIR

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    DNA damage, DNA repair

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciARA:AT2G41460-MONOMER.
    ARA:GQT-950-MONOMER.
    ReactomeiREACT_77052. Displacement of DNA glycosylase by APE1.
    REACT_90136. Base-free sugar-phosphate removal via the single-nucleotide replacement pathway.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Apurinic endonuclease-redox protein
    Alternative name(s):
    DNA-(apurinic or apyrimidinic site) lyase (EC:4.2.99.18)
    Gene namesi
    Name:ARP
    Synonyms:REF
    Ordered Locus Names:At2g41460
    ORF Names:T26J13.5
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G41460.

    Subcellular locationi

    GO - Cellular componenti

    1. chloroplast nucleoid Source: TAIR
    2. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 536536Apurinic endonuclease-redox proteinPRO_0000200018Add
    BLAST

    Proteomic databases

    PaxDbiP45951.
    PRIDEiP45951.

    Expressioni

    Tissue specificityi

    Expressed in the siliques, flowers, and stems. A high level expression is seen in the leaves.

    Gene expression databases

    ArrayExpressiP45951.
    GenevestigatoriP45951.

    Structurei

    3D structure databases

    ProteinModelPortaliP45951.
    SMRiP45951. Positions 241-536.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini97 – 13135SAPPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 278278Highly charged; increases the affinity of ARP for DNAAdd
    BLAST
    Regioni279 – 536258AP endonucleaseAdd
    BLAST

    Sequence similaritiesi

    Belongs to the DNA repair enzymes AP/ExoA family.Curated
    Contains 1 SAP domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0708.
    InParanoidiP45951.
    KOiK01142.
    OMAiQRENFDI.
    PhylomeDBiP45951.

    Family and domain databases

    Gene3Di1.10.720.30. 1 hit.
    3.60.10.10. 1 hit.
    InterProiIPR004808. AP_endonuc_1.
    IPR020847. AP_endonuclease_F1_BS.
    IPR020848. AP_endonuclease_F1_CS.
    IPR005135. Endo/exonuclease/phosphatase.
    IPR003034. SAP_dom.
    [Graphical view]
    PANTHERiPTHR22748. PTHR22748. 1 hit.
    PfamiPF03372. Exo_endo_phos. 1 hit.
    PF02037. SAP. 1 hit.
    [Graphical view]
    SMARTiSM00513. SAP. 1 hit.
    [Graphical view]
    SUPFAMiSSF56219. SSF56219. 1 hit.
    TIGRFAMsiTIGR00633. xth. 1 hit.
    PROSITEiPS00726. AP_NUCLEASE_F1_1. 1 hit.
    PS00727. AP_NUCLEASE_F1_2. 1 hit.
    PS00728. AP_NUCLEASE_F1_3. 1 hit.
    PS51435. AP_NUCLEASE_F1_4. 1 hit.
    PS50800. SAP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    This entry describes 1 isoform i produced by alternative splicing. Align

    Note: A number of isoforms are produced. According to EST sequences.

    Isoform 1 (identifier: P45951-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MNNVLQFGLQ SSAIYVAKFL VVPLRSLRVG SSFVGVGVGT RSFNKRLMSN    50
    ATAFSINNSK RKELKIPGAA IDQNCHQMGS DTDRDEMGTL QDDRKEIEAM 100
    TVQELRSTLR KLGVPVKGRK QELISTLRLH MDSNLPDQKE TSSSTRSDSV 150
    TIKRKISNRE EPTEDECTNS EAYDIEHGEK RVKQSTEKNL KAKVSAKAIA 200
    KEQKSLMRTG KQQIQSKEET SSTISSELLK TEEIISSPSQ SEPWTVLAHK 250
    KPQKDWKAYN PKTMRPPPLP EGTKCVKVMT WNVNGLRGLL KFESFSALQL 300
    AQRENFDILC LQETKLQVKD VEEIKKTLID GYDHSFWSCS VSKLGYSGTA 350
    IISRIKPLSV RYGTGLSGHD TEGRIVTAEF DSFYLINTYV PNSGDGLKRL 400
    SYRIEEWDRT LSNHIKELEK SKPVVLTGDL NCAHEEIDIF NPAGNKRSAG 450
    FTIEERQSFG ANLLDKGFVD TFRKQHPGVV GYTYWGYRHG GRKTNKGWRL 500
    DYFLVSQSIA ANVHDSYILP DINGSDHCPI GLILKL 536
    Length:536
    Mass (Da):60,260
    Last modified:May 30, 2000 - v2
    Checksum:i5C1FC17EA991D27B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC004625 Genomic DNA. Translation: AAC23731.1.
    CP002685 Genomic DNA. Translation: AEC09984.1.
    X76912 mRNA. Translation: CAA54234.1.
    PIRiT02441.
    RefSeqiNP_181677.1. NM_129709.4. [P45951-1]
    UniGeneiAt.25567.

    Genome annotation databases

    EnsemblPlantsiAT2G41460.1; AT2G41460.1; AT2G41460. [P45951-1]
    GeneIDi818744.
    KEGGiath:AT2G41460.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC004625 Genomic DNA. Translation: AAC23731.1 .
    CP002685 Genomic DNA. Translation: AEC09984.1 .
    X76912 mRNA. Translation: CAA54234.1 .
    PIRi T02441.
    RefSeqi NP_181677.1. NM_129709.4. [P45951-1 ]
    UniGenei At.25567.

    3D structure databases

    ProteinModelPortali P45951.
    SMRi P45951. Positions 241-536.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi P45951.
    PRIDEi P45951.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G41460.1 ; AT2G41460.1 ; AT2G41460 . [P45951-1 ]
    GeneIDi 818744.
    KEGGi ath:AT2G41460.

    Organism-specific databases

    TAIRi AT2G41460.

    Phylogenomic databases

    eggNOGi COG0708.
    InParanoidi P45951.
    KOi K01142.
    OMAi QRENFDI.
    PhylomeDBi P45951.

    Enzyme and pathway databases

    BioCyci ARA:AT2G41460-MONOMER.
    ARA:GQT-950-MONOMER.
    Reactomei REACT_77052. Displacement of DNA glycosylase by APE1.
    REACT_90136. Base-free sugar-phosphate removal via the single-nucleotide replacement pathway.

    Miscellaneous databases

    PROi P45951.

    Gene expression databases

    ArrayExpressi P45951.
    Genevestigatori P45951.

    Family and domain databases

    Gene3Di 1.10.720.30. 1 hit.
    3.60.10.10. 1 hit.
    InterProi IPR004808. AP_endonuc_1.
    IPR020847. AP_endonuclease_F1_BS.
    IPR020848. AP_endonuclease_F1_CS.
    IPR005135. Endo/exonuclease/phosphatase.
    IPR003034. SAP_dom.
    [Graphical view ]
    PANTHERi PTHR22748. PTHR22748. 1 hit.
    Pfami PF03372. Exo_endo_phos. 1 hit.
    PF02037. SAP. 1 hit.
    [Graphical view ]
    SMARTi SM00513. SAP. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56219. SSF56219. 1 hit.
    TIGRFAMsi TIGR00633. xth. 1 hit.
    PROSITEi PS00726. AP_NUCLEASE_F1_1. 1 hit.
    PS00727. AP_NUCLEASE_F1_2. 1 hit.
    PS00728. AP_NUCLEASE_F1_3. 1 hit.
    PS51435. AP_NUCLEASE_F1_4. 1 hit.
    PS50800. SAP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "The Arabidopsis thaliana apurinic endonuclease Arp reduces human transcription factors Fos and Jun."
      Babiychuk E., Kushnir S., van Montagu M., Inze D.
      Proc. Natl. Acad. Sci. U.S.A. 91:3299-3303(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 10-536.
      Strain: cv. Columbia.
      Tissue: Callus.

    Entry informationi

    Entry nameiARP_ARATH
    AccessioniPrimary (citable) accession number: P45951
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: May 30, 2000
    Last modified: October 1, 2014
    This is version 127 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3