Reviewed, UniProtKB/Swiss-Prot P45947 (ARSC_BACSU)

Names and origin

Protein names

Recommended name:
    Protein arsC
Alternative name(s):
    Arsenate reductase
    EC=1.20.4.-
    Arsenical pump modifier
    Low molecular weight protein-tyrosine-phosphatase
    EC=3.1.3.48

Gene names

Name:	arsC
Synonyms:	yqcM
Ordered Locus Names:	BSU25780

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	139 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Reduces arsenate [As(V)] to arsenite [As(III)] and dephosphorylates tyrosine phosphorylated proteins, low-MW aryl phosphates and natural and synthetic acyl phosphates. Could switch between different functions in different circumstances By similarity.
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate. HAMAP MF_01624 Arsenate + thioredoxin = arsenite + thioredoxin disulfide + H2O. HAMAP MF_01624
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the low molecular weight phosphotyrosine protein phosphatase superfamily. ArsC family.

Ontologies

Keywords
Biological process	Arsenical resistance
Domain	Redox-active center
Molecular function	Hydrolase Oxidoreductase
PTM	Disulfide bond
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW protein amino acid dephosphorylation Inferred from electronic annotation. Source: InterPro response to arsenic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	arsenate reductase (thioredoxin) activity Inferred from electronic annotation. Source: HAMAP protein tyrosine phosphatase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 139

139

Protein arsC HAMAP MF_01624

PRO_0000162520

Sites

Active site

10

1

Nucleophile; for reductase activity and phosphatase activity By similarity

Active site

82

1

Nucleophile; for reductase activity By similarity

Active site

89

1

Nucleophile; for reductase activity By similarity

Amino acid modifications

Disulfide bond

10 ↔ 82

Redox-active; alternate By similarity

Disulfide bond

82 ↔ 89

Redox-active; alternate By similarity

Secondary structure

1

.

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139

Helix Strand Turn

Details...

Beta strand

4 – 15

12

Helix

16 – 27

12

Beta strand

32 – 40

9

Helix

46 – 54

9

Helix

69 – 72

4

Beta strand

76 – 80

5

Helix

83 – 88

6

Beta strand

96 – 100

5

Helix

106 – 108

3

Helix

113 – 137

25

Sequences

	Sequence		Length	Mass (Da)
	P45947-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 880EE1C77D1FE130	FASTA	139	15,595
	10 20 30 40 50 60 MENKIIYFLC TGNSCRSQMA EGWAKQYLGD EWKVYSAGIE AHGLNPNAVK AMKEVGIDIS 70 80 90 100 110 120 NQTSDIIDSD ILNNADLVVT LCGDAADKCP MTPPHVKREH WGFDDPARAQ GTEEEKWAFF 130 QRVRDEIGNR LKEFAETGK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of a skin element excised by DNA rearrangement during sporulation in Bacillus subtilis." Takemaru K., Mizuno M., Sato T., Takeuchi M., Kobayashi Y. Microbiology 141:323-327(1995) [PubMed: 7704261] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168 / JH642.
[2]	"Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the Bacillus subtilis genome containing the skin element and many sporulation genes." Mizuno M., Masuda S., Takemaru K., Hosono S., Sato T., Takeuchi M., Kobayashi Y. Microbiology 142:3103-3111(1996) [PubMed: 8969508] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168 / JH642.
[3]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[4]	"Analysis of a Bacillus subtilis genome fragment using a co-operative computer system prototype." Medigue C., Moszer I., Viari A., Danchin A. Gene 165:GC37-GC51(1995) [PubMed: 7489895] [Abstract] Cited for: IDENTIFICATION.
[5]	"The ars operon in the skin element of Bacillus subtilis confers resistance to arsenate and arsenite." Sato T., Kobayashi Y. J. Bacteriol. 180:1655-1661(1998) [PubMed: 9537360] [Abstract] Cited for: CHARACTERIZATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

D32216 Genomic DNA. Translation: BAA06970.1.
D84432 Genomic DNA. Translation: BAA12434.1.
AL009126 Genomic DNA. Translation: CAB14519.1.

PIR

C69950.

RefSeq

NP_390455.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1JL3	X-ray	1.60	A/B/C/D	1-139	[»]
1Z2D	NMR	-	A	1-139	[»]
1Z2E	NMR	-	A	1-139	[»]
2IPA	NMR	-	B	1-139	[»]

ModBase

Search...

Genome annotation databases

GeneID

937801.

GenomeReviews

Gene locus BSU25780 in contig AL009126_GR.

KEGG

bsu:BSU25780.

NMPDR

fig|224308.1.peg.2580.

Organism-specific databases

SubtiList

BG11304. arsC. [Micado]

CMR

Search...

Phylogenomic databases

HOGENOM

P45947.

OMA

P45947. EMRIVIT.

Enzyme and pathway databases

BioCyc

BSUB224308:BSU2573-MON.

BRENDA

3.1.3.48. 150.

Family and domain databases

HAMAP

MF_01624.
[Tree]

InterPro

IPR014064. Arsenate_reductase_StaphA.
IPR017867. Tyr_phospatase_low_mol_wt.
[Graphical view]

PANTHER

PTHR11717. Low_mwt_PTPase. 1 hit.

Pfam

PF01451. LMWPc. 1 hit.
[Graphical view]

SMART

SM00226. LMWPc. 1 hit.
[Graphical view]

TIGRFAMs

TIGR02691. arsC_pI258_fam. 1 hit.

ProtoNet

Search...

Entry information

Entry name

ARSC_BACSU

Accession

Primary (citable) accession number: P45947

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	June 16, 2009
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)