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Protein

Arsenate reductase

Gene

arsC

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reduction of arsenate [As(V)] to arsenite [As(III)] (PubMed:16797027). In vitro, can dephosphorylate para-nitrophenyl phosphate (pNPP) (PubMed:11698660).2 Publications

Catalytic activityi

Arsenate + thioredoxin = arsenite + thioredoxin disulfide + H2O.UniRule annotation1 Publication

Enzyme regulationi

Activity is potassium and sulfate-independent.1 Publication

Kineticsi

kcat is 96 min(-1) in the presence of KCl (PubMed:16797027). kcat is 80 min(-1) in the presence of NaCl (PubMed:16797027). kcat is 120 min(-1) in the presence of Na2SO4 (PubMed:16797027). kcat is 95 min(-1) in the presence of K2SO4 (PubMed:16797027).1 Publication
  1. KM=54 µM for arsenate (in the presence of KCl)1 Publication
  2. KM=58 µM for arsenate (in the presence of NaCl)1 Publication
  3. KM=64 µM for arsenate (in the presence of Na2SO4)1 Publication
  4. KM=47 µM for arsenate (in the presence of K2SO4)1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei10NucleophileUniRule annotation1 Publication1
    Active sitei82NucleophileUniRule annotation1 Publication1
    Active sitei89NucleophileUniRule annotation1 Publication1

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionOxidoreductase
    Biological processArsenical resistance

    Enzyme and pathway databases

    BioCyciBSUB:BSU25780-MONOMER.
    BRENDAi1.20.4.B2. 658.
    SABIO-RKiP45947.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Arsenate reductase1 PublicationUniRule annotation (EC:1.20.4.4UniRule annotation1 Publication)
    Alternative name(s):
    Arsenical pump modifier
    Protein ArsC
    Gene namesi
    Name:arsC1 PublicationUniRule annotation
    Synonyms:yqcM
    Ordered Locus Names:BSU25780
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    Proteomesi
    • UP000001570 Componenti: Chromosome

    Subcellular locationi

    • Cytoplasm UniRule annotation

    GO - Cellular componenti

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Disruption phenotypei

    Mutant is sensitive to arsenate but is still resistant to arsenite.1 Publication

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi33K → N: Binds potassium in the micromolar range. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001625201 – 139Arsenate reductaseAdd BLAST139

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Disulfide bondi10 ↔ 82Redox-active; alternateUniRule annotation1 Publication
    Disulfide bondi82 ↔ 89Redox-active; alternateUniRule annotationCombined sources1 Publication

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    PaxDbiP45947.

    Expressioni

    Inductioni

    Induced by arsenate, arsenite, and antimonite.1 Publication

    Interactioni

    Subunit structurei

    Monomer.1 Publication

    Protein-protein interaction databases

    STRINGi224308.Bsubs1_010100014101.

    Structurei

    Secondary structure

    1139
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi4 – 15Combined sources12
    Helixi16 – 27Combined sources12
    Turni28 – 30Combined sources3
    Beta strandi32 – 40Combined sources9
    Helixi46 – 54Combined sources9
    Helixi59 – 61Combined sources3
    Helixi69 – 72Combined sources4
    Beta strandi76 – 80Combined sources5
    Helixi83 – 88Combined sources6
    Beta strandi96 – 100Combined sources5
    Helixi106 – 108Combined sources3
    Helixi113 – 137Combined sources25

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1JL3X-ray1.60A/B/C/D1-139[»]
    1Z2DNMR-A1-139[»]
    1Z2ENMR-A1-139[»]
    2IPANMR-B1-139[»]
    ProteinModelPortaliP45947.
    SMRiP45947.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP45947.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the low molecular weight phosphotyrosine protein phosphatase family. Thioredoxin-coupled ArsC subfamily.UniRule annotationCurated

    Keywords - Domaini

    Redox-active center

    Phylogenomic databases

    eggNOGiENOG4108UXE. Bacteria.
    COG0394. LUCA.
    HOGENOMiHOG000273093.
    InParanoidiP45947.
    KOiK03741.
    OMAiCSHADSV.
    PhylomeDBiP45947.

    Family and domain databases

    HAMAPiMF_01624. Arsenate_reduct. 1 hit.
    InterProiView protein in InterPro
    IPR014064. Arsenate_reductase_ArsC.
    IPR023485. Ptyr_pPase_SF.
    PfamiView protein in Pfam
    PF01451. LMWPc. 1 hit.
    SMARTiView protein in SMART
    SM00226. LMWPc. 1 hit.
    SUPFAMiSSF52788. SSF52788. 1 hit.
    TIGRFAMsiTIGR02691. arsC_pI258_fam. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P45947-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MENKIIYFLC TGNSCRSQMA EGWAKQYLGD EWKVYSAGIE AHGLNPNAVK
    60 70 80 90 100
    AMKEVGIDIS NQTSDIIDSD ILNNADLVVT LCGDAADKCP MTPPHVKREH
    110 120 130
    WGFDDPARAQ GTEEEKWAFF QRVRDEIGNR LKEFAETGK
    Length:139
    Mass (Da):15,595
    Last modified:November 1, 1995 - v1
    Checksum:i880EE1C77D1FE130
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    D32216 Genomic DNA. Translation: BAA06970.1.
    D84432 Genomic DNA. Translation: BAA12434.1.
    AL009126 Genomic DNA. Translation: CAB14519.1.
    PIRiC69950.
    RefSeqiNP_390455.1. NC_000964.3.
    WP_004398596.1. NZ_JNCM01000036.1.

    Genome annotation databases

    EnsemblBacteriaiCAB14519; CAB14519; BSU25780.
    GeneIDi937801.
    KEGGibsu:BSU25780.
    PATRICifig|224308.179.peg.2802.

    Similar proteinsi

    Entry informationi

    Entry nameiARSC_BACSU
    AccessioniPrimary (citable) accession number: P45947
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: September 27, 2017
    This is version 131 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families