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P45947

- ARSC_BACSU

UniProt

P45947 - ARSC_BACSU

Protein

Protein ArsC

Gene

arsC

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Reduces arsenate [As(V)] to arsenite [As(III)] and dephosphorylates tyrosine phosphorylated proteins, low-MW aryl phosphates and natural and synthetic acyl phosphates. Could switch between different functions in different circumstances.UniRule annotation

    Catalytic activityi

    Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.UniRule annotation
    Arsenate + thioredoxin = arsenite + thioredoxin disulfide + H2O.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei10 – 101Nucleophile; for reductase activity and phosphatase activityUniRule annotation
    Active sitei82 – 821Nucleophile; for reductase activityUniRule annotation
    Active sitei89 – 891Nucleophile; for reductase activityUniRule annotation

    GO - Molecular functioni

    1. arsenate reductase (thioredoxin) activity Source: UniProtKB-HAMAP
    2. protein tyrosine phosphatase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. response to arsenic-containing substance Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase, Oxidoreductase

    Keywords - Biological processi

    Arsenical resistance

    Enzyme and pathway databases

    BioCyciBSUB:BSU25780-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein ArsCUniRule annotation
    Alternative name(s):
    Arsenate reductaseUniRule annotation (EC:1.20.4.-UniRule annotation)
    Arsenical pump modifierUniRule annotation
    Low molecular weight protein-tyrosine-phosphataseUniRule annotation (EC:3.1.3.48UniRule annotation)
    Gene namesi
    Name:arsCUniRule annotation
    Synonyms:yqcM
    Ordered Locus Names:BSU25780
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000001570: Chromosome

    Organism-specific databases

    GenoListiBSU25780. [Micado]

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 139139Protein ArsCPRO_0000162520Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi10 ↔ 82Redox-active; alternateUniRule annotation
    Disulfide bondi82 ↔ 89Redox-active; alternateUniRule annotation

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    PaxDbiP45947.

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi224308.BSU25780.

    Structurei

    Secondary structure

    1
    139
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi4 – 1512
    Helixi16 – 2712
    Turni28 – 303
    Beta strandi32 – 409
    Helixi46 – 549
    Helixi59 – 613
    Helixi69 – 724
    Beta strandi76 – 805
    Helixi83 – 886
    Beta strandi96 – 1005
    Helixi106 – 1083
    Helixi113 – 13725

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1JL3X-ray1.60A/B/C/D1-139[»]
    1Z2DNMR-A1-139[»]
    1Z2ENMR-A1-139[»]
    2IPANMR-B1-139[»]
    ProteinModelPortaliP45947.
    SMRiP45947. Positions 3-139.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP45947.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the low molecular weight phosphotyrosine protein phosphatase superfamily. ArsC family.UniRule annotation

    Keywords - Domaini

    Redox-active center

    Phylogenomic databases

    eggNOGiCOG0394.
    HOGENOMiHOG000273093.
    KOiK03741.
    OMAiEMRIVIT.
    OrthoDBiEOG6JDWJC.
    PhylomeDBiP45947.

    Family and domain databases

    HAMAPiMF_01624. Arsenate_reduct.
    InterProiIPR014064. Arsenate_reductase_ArsC.
    IPR023485. Ptyr_pPase_SF.
    IPR017867. Tyr_phospatase_low_mol_wt.
    [Graphical view]
    PANTHERiPTHR11717. PTHR11717. 1 hit.
    PfamiPF01451. LMWPc. 1 hit.
    [Graphical view]
    SMARTiSM00226. LMWPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52788. SSF52788. 1 hit.
    TIGRFAMsiTIGR02691. arsC_pI258_fam. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P45947-1 [UniParc]FASTAAdd to Basket

    « Hide

    MENKIIYFLC TGNSCRSQMA EGWAKQYLGD EWKVYSAGIE AHGLNPNAVK    50
    AMKEVGIDIS NQTSDIIDSD ILNNADLVVT LCGDAADKCP MTPPHVKREH 100
    WGFDDPARAQ GTEEEKWAFF QRVRDEIGNR LKEFAETGK 139
    Length:139
    Mass (Da):15,595
    Last modified:November 1, 1995 - v1
    Checksum:i880EE1C77D1FE130
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D32216 Genomic DNA. Translation: BAA06970.1.
    D84432 Genomic DNA. Translation: BAA12434.1.
    AL009126 Genomic DNA. Translation: CAB14519.1.
    PIRiC69950.
    RefSeqiNP_390455.1. NC_000964.3.
    WP_004398596.1. NZ_CM000487.1.

    Genome annotation databases

    EnsemblBacteriaiCAB14519; CAB14519; BSU25780.
    GeneIDi937801.
    KEGGibsu:BSU25780.
    PATRICi18977000. VBIBacSub10457_2689.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D32216 Genomic DNA. Translation: BAA06970.1 .
    D84432 Genomic DNA. Translation: BAA12434.1 .
    AL009126 Genomic DNA. Translation: CAB14519.1 .
    PIRi C69950.
    RefSeqi NP_390455.1. NC_000964.3.
    WP_004398596.1. NZ_CM000487.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1JL3 X-ray 1.60 A/B/C/D 1-139 [» ]
    1Z2D NMR - A 1-139 [» ]
    1Z2E NMR - A 1-139 [» ]
    2IPA NMR - B 1-139 [» ]
    ProteinModelPortali P45947.
    SMRi P45947. Positions 3-139.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224308.BSU25780.

    Proteomic databases

    PaxDbi P45947.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAB14519 ; CAB14519 ; BSU25780 .
    GeneIDi 937801.
    KEGGi bsu:BSU25780.
    PATRICi 18977000. VBIBacSub10457_2689.

    Organism-specific databases

    GenoListi BSU25780. [Micado ]

    Phylogenomic databases

    eggNOGi COG0394.
    HOGENOMi HOG000273093.
    KOi K03741.
    OMAi EMRIVIT.
    OrthoDBi EOG6JDWJC.
    PhylomeDBi P45947.

    Enzyme and pathway databases

    BioCyci BSUB:BSU25780-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P45947.

    Family and domain databases

    HAMAPi MF_01624. Arsenate_reduct.
    InterProi IPR014064. Arsenate_reductase_ArsC.
    IPR023485. Ptyr_pPase_SF.
    IPR017867. Tyr_phospatase_low_mol_wt.
    [Graphical view ]
    PANTHERi PTHR11717. PTHR11717. 1 hit.
    Pfami PF01451. LMWPc. 1 hit.
    [Graphical view ]
    SMARTi SM00226. LMWPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52788. SSF52788. 1 hit.
    TIGRFAMsi TIGR02691. arsC_pI258_fam. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Complete nucleotide sequence of a skin element excised by DNA rearrangement during sporulation in Bacillus subtilis."
      Takemaru K., Mizuno M., Sato T., Takeuchi M., Kobayashi Y.
      Microbiology 141:323-327(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168 / JH642.
    2. "Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the Bacillus subtilis genome containing the skin element and many sporulation genes."
      Mizuno M., Masuda S., Takemaru K., Hosono S., Sato T., Takeuchi M., Kobayashi Y.
      Microbiology 142:3103-3111(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168 / JH642.
    3. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
      Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
      , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
      Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 168.
    4. "Analysis of a Bacillus subtilis genome fragment using a co-operative computer system prototype."
      Medigue C., Moszer I., Viari A., Danchin A.
      Gene 165:GC37-GC51(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION.
    5. "The ars operon in the skin element of Bacillus subtilis confers resistance to arsenate and arsenite."
      Sato T., Kobayashi Y.
      J. Bacteriol. 180:1655-1661(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.

    Entry informationi

    Entry nameiARSC_BACSU
    AccessioniPrimary (citable) accession number: P45947
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 112 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3