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Protein

Chondroitin sulfate synthase mig-22

Gene

mig-22

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Has both beta-1,3-glucuronic acid and beta-1,4-N-acetylgalactosamine transferase activity. Transfers glucuronic acid (GlcUA) from UDP-GlcUA and N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the non-reducing end of the elongating chondroitin polymer (By similarity). Required together with sqv-5 for the biosynthesis of chondroitin (PubMed:15485872). Chondroitin is involved in organogenesis of the vulva, maturation of the gonad, and neural development (PubMed:15485872, PubMed:16982046). May have a specific role in unc-6/netrin-mediated dorsal guidance of gonadal distal tip cells (PubMed:16982046). Glycosyltransferase activity is weak (PubMed:15485872).1 PublicationBy similarity2 Publications

Catalytic activityi

UDP-N-acetyl-alpha-D-galactosamine + [protein]-3-O-(beta-D-GlcA-(1->3)-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine = UDP + [protein]-3-O-(beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine.
UDP-N-acetyl-alpha-D-galactosamine + [protein]-3-O-(beta-D-GlcA-(1->3)-(beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3))(n)-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine = UDP + [protein]-3-O-((beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3))(n+1)-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine.
UDP-alpha-D-glucuronate + N-acetyl-beta-D-galactosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan = UDP + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan.

Cofactori

GO - Molecular functioni

Keywordsi

Molecular functionTransferase

Protein family/group databases

CAZyiGT31 Glycosyltransferase Family 31
GT7 Glycosyltransferase Family 7

Names & Taxonomyi

Protein namesi
Recommended name:
Chondroitin sulfate synthase mig-22 (EC:2.4.1.175)
Alternative name(s):
Abnormal cell migration
Chondroitin-polymerizing factor1 Publication
Short name:
ChPF1 Publication
N-acetylgalactosaminyl-proteoglycan 3-beta-glucuronosyltransferase 1 (EC:2.4.1.226)
Gene namesi
Name:mig-22
Synonyms:pfc-1
ORF Names:PAR2.4
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome III

Organism-specific databases

WormBaseiPAR2.4a ; CE00848 ; WBGene00003253 ; mig-22
PAR2.4b ; CE32699 ; WBGene00003253 ; mig-22

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 6CytoplasmicSequence analysis6
Transmembranei7 – 27Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini28 – 804LumenalSequence analysisAdd BLAST777

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Disruption phenotypei

Viable but sterile, with reversion of cytokinesis during early embryogenesis.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000654151 – 804Chondroitin sulfate synthase mig-22Add BLAST804

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi123N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi172N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi268N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

EPDiP45895
PaxDbiP45895
PeptideAtlasiP45895
PRIDEiP45895

Expressioni

Tissue specificityi

Expressed in seam cells, the vulval epithelium and in oocytes (at protein level).2 Publications

Developmental stagei

Expressed in distal tip cells of the growing gonad arms from L3 to the young adult stage (at protein level).1 Publication

Gene expression databases

BgeeiWBGene00003253

Interactioni

Subunit structurei

Interacts with sqv-5.1 Publication

GO - Molecular functioni

  • enzyme binding Source: WormBase

Protein-protein interaction databases

BioGridi41434, 2 interactors
IntActiP45895, 1 interactor
STRINGi6239.PAR2.4a.1

Structurei

3D structure databases

ProteinModelPortaliP45895
SMRiP45895
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3708 Eukaryota
ENOG410XWZJ LUCA
GeneTreeiENSGT00760000119143
HOGENOMiHOG000018140
InParanoidiP45895
KOiK00747
OMAiILPMPYV
OrthoDBiEOG091G04Y5
PhylomeDBiP45895

Family and domain databases

InterProiView protein in InterPro
IPR008428 Chond_GalNAc
PfamiView protein in Pfam
PF05679 CHGN, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform a (identifier: P45895-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVGGGRTGIH LLLGFLIGAA LALFFFSSTP SIDLTSSLAA FTSCQNQETE
60 70 80 90 100
TNVLEPSALE KGRVYKDLSE HWIVHQDDMP APPHNQDATP KVTRTRFAAT
110 120 130 140 150
ELGTRERVMA AVMAESALAL SINATLGRHV PRVHLFADSS RIDNDLAQLT
160 170 180 190 200
NLSPYKLNGQ KTHSMVLGLL FNMTVHNNYD WFLLAKDSTY INPFVLLRMI
210 220 230 240 250
DTMNWNEPVV MGEAAEDGSG RCRLDTGMLL SQPAMHALMN NRNACNNFAL
260 270 280 290 300
AADDDQLAFE KCIQIATNLT CKPLHQGVRY EVWRGAERAD SPAAHDSIED
310 320 330 340 350
WKHSPAFKRA LAVPRLLSDA DASALHDYFV RVEMQRADRE IIKMEAELSR
360 370 380 390 400
LAEQEARETG EAISWPPALP PYAKPPNRYQ VSTWEYFTMT ELFRSEPNQN
410 420 430 440 450
VRRLEGKDFD DVAEVVVAAR QQVESEEPEL EFVQLRNGYR VFDPRRGMDY
460 470 480 490 500
MVDLTYRKTV NEMPEVDNRF ESDNEAAHEE SLKEIVVERR VHVSRMIAST
510 520 530 540 550
QLMNQAPYVK EDTDVTVVIP VASEKDVLPA RKLLARQARL CLFPTEEARK
560 570 580 590 600
TRMVVAVFPL IESRSVTAIT NDMEELKRRC KRSLLETDVL PVHPAVSTEG
610 620 630 640 650
KGTAAAAALD DAVDRYGANT IYLLLSPHAD VQKEFFDRAR INTIKHYQVF
660 670 680 690 700
FPVPFVEYHP TISGMEMTEK EEKETPTEQA REAALSRLRD GVEPKRKRTL
710 720 730 740 750
IVQKEHGRFD SQDFSCFAVY GVDYVTARAK FGQNERRNDL ISAFLGQDSI
760 770 780 790 800
HVLRAVEPTL RIRYHKRSCD MESIDTEDIA RCLDSKKENV AAKDQLAKLL

FHEK
Length:804
Mass (Da):91,036
Last modified:November 1, 1995 - v1
Checksum:iC799999A4C4C6314
GO
Isoform b (identifier: P45895-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-198: Missing.

Show »
Length:606
Mass (Da):69,200
Checksum:iD93977F40E22A1CA
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0197781 – 198Missing in isoform b. 1 PublicationAdd BLAST198

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB110823 mRNA Translation: BAD80738.1
AB110824 mRNA Translation: BAD80739.1
FO081577 Genomic DNA Translation: CCD72535.1
FO081577 Genomic DNA Translation: CCD72536.1
PIRiS44860
RefSeqiNP_498934.1, NM_066533.4 [P45895-1]
NP_871671.1, NM_181942.3 [P45895-2]
UniGeneiCel.7531

Genome annotation databases

EnsemblMetazoaiPAR2.4a.1; PAR2.4a.1; WBGene00003253 [P45895-1]
PAR2.4a.2; PAR2.4a.2; WBGene00003253 [P45895-1]
PAR2.4a.3; PAR2.4a.3; WBGene00003253 [P45895-1]
GeneIDi176231
KEGGicel:CELE_PAR2.4

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiCHSSB_CAEEL
AccessioniPrimary (citable) accession number: P45895
Secondary accession number(s): Q5NUN9, Q8IG00
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: May 23, 2018
This is version 110 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

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