Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Chondroitin sulfate synthase mig-22

Gene

mig-22

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has both beta-1,3-glucuronic acid and beta-1,4-N-acetylgalactosamine transferase activity. Transfers glucuronic acid (GlcUA) from UDP-GlcUA and N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the non-reducing end of the elongating chondroitin polymer (By similarity). Required together with sqv-5 for the biosynthesis of chondroitin (PubMed:15485872). Chondroitin is involved in organogenesis of the vulva, maturation of the gonad, and neural development (PubMed:15485872, PubMed:16982046). May have a specific role in unc-6/netrin-mediated dorsal guidance of gonadal distal tip cells (PubMed:16982046). Glycosyltransferase activity is weak (PubMed:15485872).1 PublicationBy similarity2 Publications

Catalytic activityi

UDP-N-acetyl-alpha-D-galactosamine + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-proteoglycan = UDP + N-acetyl-beta-D-galactosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-proteoglycan.
UDP-alpha-D-glucuronate + N-acetyl-beta-D-galactosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan = UDP + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan.

Cofactori

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Enzyme and pathway databases

ReactomeiR-CEL-2022870. Chondroitin sulfate biosynthesis.

Protein family/group databases

CAZyiGT31. Glycosyltransferase Family 31.
GT7. Glycosyltransferase Family 7.

Names & Taxonomyi

Protein namesi
Recommended name:
Chondroitin sulfate synthase mig-22 (EC:2.4.1.175)
Alternative name(s):
Abnormal cell migration
Chondroitin-polymerizing factor1 Publication
Short name:
ChPF1 Publication
N-acetylgalactosaminyl-proteoglycan 3-beta-glucuronosyltransferase 1 (EC:2.4.1.226)
Gene namesi
Name:mig-22
Synonyms:pfc-1
ORF Names:PAR2.4
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome III

Organism-specific databases

WormBaseiPAR2.4a; CE00848; WBGene00003253; mig-22.
PAR2.4b; CE32699; WBGene00003253; mig-22.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 66CytoplasmicSequence analysis
Transmembranei7 – 2721Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini28 – 804777LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Disruption phenotypei

Viable but sterile, with reversion of cytokinesis during early embryogenesis.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 804804Chondroitin sulfate synthase mig-22PRO_0000065415Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi123 – 1231N-linked (GlcNAc...)Sequence analysis
Glycosylationi172 – 1721N-linked (GlcNAc...)Sequence analysis
Glycosylationi268 – 2681N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

EPDiP45895.
PaxDbiP45895.
PRIDEiP45895.

Expressioni

Tissue specificityi

Expressed in seam cells, the vulval epithelium and in oocytes (at protein level).2 Publications

Developmental stagei

Expressed in distal tip cells of the growing gonad arms from L3 to the young adult stage (at protein level).1 Publication

Gene expression databases

BgeeiWBGene00003253.

Interactioni

GO - Molecular functioni

  • enzyme binding Source: WormBase

Protein-protein interaction databases

BioGridi41434. 2 interactions.
IntActiP45895. 1 interaction.
STRINGi6239.PAR2.4a.1.

Structurei

3D structure databases

ProteinModelPortaliP45895.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3708. Eukaryota.
ENOG410XWZJ. LUCA.
GeneTreeiENSGT00760000119143.
HOGENOMiHOG000018140.
InParanoidiP45895.
KOiK00747.
OMAiDFSCFAV.
OrthoDBiEOG091G04Y5.
PhylomeDBiP45895.

Family and domain databases

InterProiIPR008428. Chond_GalNAc.
[Graphical view]
PfamiPF05679. CHGN. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform a (identifier: P45895-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVGGGRTGIH LLLGFLIGAA LALFFFSSTP SIDLTSSLAA FTSCQNQETE
60 70 80 90 100
TNVLEPSALE KGRVYKDLSE HWIVHQDDMP APPHNQDATP KVTRTRFAAT
110 120 130 140 150
ELGTRERVMA AVMAESALAL SINATLGRHV PRVHLFADSS RIDNDLAQLT
160 170 180 190 200
NLSPYKLNGQ KTHSMVLGLL FNMTVHNNYD WFLLAKDSTY INPFVLLRMI
210 220 230 240 250
DTMNWNEPVV MGEAAEDGSG RCRLDTGMLL SQPAMHALMN NRNACNNFAL
260 270 280 290 300
AADDDQLAFE KCIQIATNLT CKPLHQGVRY EVWRGAERAD SPAAHDSIED
310 320 330 340 350
WKHSPAFKRA LAVPRLLSDA DASALHDYFV RVEMQRADRE IIKMEAELSR
360 370 380 390 400
LAEQEARETG EAISWPPALP PYAKPPNRYQ VSTWEYFTMT ELFRSEPNQN
410 420 430 440 450
VRRLEGKDFD DVAEVVVAAR QQVESEEPEL EFVQLRNGYR VFDPRRGMDY
460 470 480 490 500
MVDLTYRKTV NEMPEVDNRF ESDNEAAHEE SLKEIVVERR VHVSRMIAST
510 520 530 540 550
QLMNQAPYVK EDTDVTVVIP VASEKDVLPA RKLLARQARL CLFPTEEARK
560 570 580 590 600
TRMVVAVFPL IESRSVTAIT NDMEELKRRC KRSLLETDVL PVHPAVSTEG
610 620 630 640 650
KGTAAAAALD DAVDRYGANT IYLLLSPHAD VQKEFFDRAR INTIKHYQVF
660 670 680 690 700
FPVPFVEYHP TISGMEMTEK EEKETPTEQA REAALSRLRD GVEPKRKRTL
710 720 730 740 750
IVQKEHGRFD SQDFSCFAVY GVDYVTARAK FGQNERRNDL ISAFLGQDSI
760 770 780 790 800
HVLRAVEPTL RIRYHKRSCD MESIDTEDIA RCLDSKKENV AAKDQLAKLL

FHEK
Length:804
Mass (Da):91,036
Last modified:November 1, 1995 - v1
Checksum:iC799999A4C4C6314
GO
Isoform b (identifier: P45895-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-198: Missing.

Show »
Length:606
Mass (Da):69,200
Checksum:iD93977F40E22A1CA
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 198198Missing in isoform b. 1 PublicationVSP_019778Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB110823 mRNA. Translation: BAD80738.1.
AB110824 mRNA. Translation: BAD80739.1.
FO081577 Genomic DNA. Translation: CCD72535.1.
FO081577 Genomic DNA. Translation: CCD72536.1.
PIRiS44860.
RefSeqiNP_498934.1. NM_066533.4. [P45895-1]
NP_871671.1. NM_181942.3. [P45895-2]
UniGeneiCel.7531.

Genome annotation databases

EnsemblMetazoaiPAR2.4a.1; PAR2.4a.1; WBGene00003253. [P45895-1]
PAR2.4a.2; PAR2.4a.2; WBGene00003253. [P45895-1]
PAR2.4a.3; PAR2.4a.3; WBGene00003253. [P45895-1]
GeneIDi176231.
KEGGicel:CELE_PAR2.4.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB110823 mRNA. Translation: BAD80738.1.
AB110824 mRNA. Translation: BAD80739.1.
FO081577 Genomic DNA. Translation: CCD72535.1.
FO081577 Genomic DNA. Translation: CCD72536.1.
PIRiS44860.
RefSeqiNP_498934.1. NM_066533.4. [P45895-1]
NP_871671.1. NM_181942.3. [P45895-2]
UniGeneiCel.7531.

3D structure databases

ProteinModelPortaliP45895.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi41434. 2 interactions.
IntActiP45895. 1 interaction.
STRINGi6239.PAR2.4a.1.

Protein family/group databases

CAZyiGT31. Glycosyltransferase Family 31.
GT7. Glycosyltransferase Family 7.

Proteomic databases

EPDiP45895.
PaxDbiP45895.
PRIDEiP45895.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiPAR2.4a.1; PAR2.4a.1; WBGene00003253. [P45895-1]
PAR2.4a.2; PAR2.4a.2; WBGene00003253. [P45895-1]
PAR2.4a.3; PAR2.4a.3; WBGene00003253. [P45895-1]
GeneIDi176231.
KEGGicel:CELE_PAR2.4.

Organism-specific databases

CTDi176231.
WormBaseiPAR2.4a; CE00848; WBGene00003253; mig-22.
PAR2.4b; CE32699; WBGene00003253; mig-22.

Phylogenomic databases

eggNOGiKOG3708. Eukaryota.
ENOG410XWZJ. LUCA.
GeneTreeiENSGT00760000119143.
HOGENOMiHOG000018140.
InParanoidiP45895.
KOiK00747.
OMAiDFSCFAV.
OrthoDBiEOG091G04Y5.
PhylomeDBiP45895.

Enzyme and pathway databases

ReactomeiR-CEL-2022870. Chondroitin sulfate biosynthesis.

Miscellaneous databases

PROiP45895.

Gene expression databases

BgeeiWBGene00003253.

Family and domain databases

InterProiIPR008428. Chond_GalNAc.
[Graphical view]
PfamiPF05679. CHGN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCHSSB_CAEEL
AccessioniPrimary (citable) accession number: P45895
Secondary accession number(s): Q5NUN9, Q8IG00
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: September 7, 2016
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.