Reviewed, UniProtKB/Swiss-Prot P45895 (CHSS2_CAEEL)

Names and origin

Protein names

Recommended name:
    Chondroitin sulfate synthase 2
    EC=2.4.1.175
Alternative name(s):
    Chondroitin-polymerizing factor S
    N-acetylgalactosaminyl-proteoglycan 3-beta-glucuronosyltransferase 1
    EC=2.4.1.226
    Abnormal cell migration

Gene names

Name:	mig-22
Synonyms:	pfc-1
ORF Names:	PAR2.4

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	804 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Has both beta-1,3-glucuronic acid and beta-1,4-N-acetylgalactosamine transferase activity. Transfers glucuronic acid (GlcUA) from UDP-GlcUA and N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the non-reducing end of the elongating chondroitin polymer By similarity. Sqv-5 and pfc-1 are indispensable for the biosynthesis of chondroitin. Chondroitin is involved in the organogenesis of the vulva and maturation of the gonad and may be involved in distal tip cell migration and neural development. Glycosyltransferase activity is weak. Ref.1
Catalytic activity	UDP-N-acetyl-D-galactosamine + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-proteoglycan = UDP + N-acetyl-beta-D-galactosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-proteoglycan. UDP-alpha-D-glucuronate + N-acetyl-beta-D-galactosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan = UDP + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan.
Cofactor	Divalent cations By similarity.
Subcellular location	Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein Probable.
Tissue specificity	Expressed in seam cells. Also in vulva and distal tip cells of the growing gonad arms from L3 through to the young adult stage. Ref.1
Disruption phenotype	Reversion of cytokinesis. Ref.1
Sequence similarities	Belongs to the chondroitin N-acetylgalactosaminyltransferase family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Coding sequence diversity	Alternative splicing
Domain	Signal-anchor Transmembrane
Molecular function	Transferase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cytokinesis Ref.1 Inferred from mutant phenotype. Source: WormBase embryonic development ending in birth or egg hatching Inferred from mutant phenotype. Source: WormBase hermaphrodite genitalia development Inferred from mutant phenotype. Source: WormBase receptor-mediated endocytosis Inferred from mutant phenotype. Source: WormBase
Cellular component	Golgi cisterna membrane Inferred from electronic annotation. Source: InterPro integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	N-acetylgalactosaminyl-proteoglycan 3-beta-glucuronosyltransferase activity Inferred from electronic annotation. Source: EC glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform a (identifier: P45895-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform b (identifier: P45895-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-198: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 804	804	Chondroitin sulfate synthase 2		PRO_0000065415
Regions
	Topological domain	1 – 6	6	Cytoplasmic Potential
	Transmembrane	7 – 27	21	Signal-anchor for type II membrane protein Potential
	Topological domain	28 – 804	777	Lumenal Potential
Amino acid modifications
	Glycosylation	123	1	N-linked (GlcNAc...) Potential
	Glycosylation	172	1	N-linked (GlcNAc...) Potential
	Glycosylation	268	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	1 – 198	198	Missing in isoform b.		VSP_019778

Sequences

	Sequence		Length	Mass (Da)
	Isoform a [UniParc]. Last modified November 1, 1995. Version 1. Checksum: C799999A4C4C6314	FASTA	804	91,036
	10 20 30 40 50 60 MVGGGRTGIH LLLGFLIGAA LALFFFSSTP SIDLTSSLAA FTSCQNQETE TNVLEPSALE 70 80 90 100 110 120 KGRVYKDLSE HWIVHQDDMP APPHNQDATP KVTRTRFAAT ELGTRERVMA AVMAESALAL 130 140 150 160 170 180 SINATLGRHV PRVHLFADSS RIDNDLAQLT NLSPYKLNGQ KTHSMVLGLL FNMTVHNNYD 190 200 210 220 230 240 WFLLAKDSTY INPFVLLRMI DTMNWNEPVV MGEAAEDGSG RCRLDTGMLL SQPAMHALMN 250 260 270 280 290 300 NRNACNNFAL AADDDQLAFE KCIQIATNLT CKPLHQGVRY EVWRGAERAD SPAAHDSIED 310 320 330 340 350 360 WKHSPAFKRA LAVPRLLSDA DASALHDYFV RVEMQRADRE IIKMEAELSR LAEQEARETG 370 380 390 400 410 420 EAISWPPALP PYAKPPNRYQ VSTWEYFTMT ELFRSEPNQN VRRLEGKDFD DVAEVVVAAR 430 440 450 460 470 480 QQVESEEPEL EFVQLRNGYR VFDPRRGMDY MVDLTYRKTV NEMPEVDNRF ESDNEAAHEE 490 500 510 520 530 540 SLKEIVVERR VHVSRMIAST QLMNQAPYVK EDTDVTVVIP VASEKDVLPA RKLLARQARL 550 560 570 580 590 600 CLFPTEEARK TRMVVAVFPL IESRSVTAIT NDMEELKRRC KRSLLETDVL PVHPAVSTEG 610 620 630 640 650 660 KGTAAAAALD DAVDRYGANT IYLLLSPHAD VQKEFFDRAR INTIKHYQVF FPVPFVEYHP 670 680 690 700 710 720 TISGMEMTEK EEKETPTEQA REAALSRLRD GVEPKRKRTL IVQKEHGRFD SQDFSCFAVY 730 740 750 760 770 780 GVDYVTARAK FGQNERRNDL ISAFLGQDSI HVLRAVEPTL RIRYHKRSCD MESIDTEDIA 790 800 RCLDSKKENV AAKDQLAKLL FHEK
	Isoform b. Checksum: D93977F40E22A1CA	FASTA	606	69,200
	10 20 30 40 50 60 MIDTMNWNEP VVMGEAAEDG SGRCRLDTGM LLSQPAMHAL MNNRNACNNF ALAADDDQLA 70 80 90 100 110 120 FEKCIQIATN LTCKPLHQGV RYEVWRGAER ADSPAAHDSI EDWKHSPAFK RALAVPRLLS 130 140 150 160 170 180 DADASALHDY FVRVEMQRAD REIIKMEAEL SRLAEQEARE TGEAISWPPA LPPYAKPPNR 190 200 210 220 230 240 YQVSTWEYFT MTELFRSEPN QNVRRLEGKD FDDVAEVVVA ARQQVESEEP ELEFVQLRNG 250 260 270 280 290 300 YRVFDPRRGM DYMVDLTYRK TVNEMPEVDN RFESDNEAAH EESLKEIVVE RRVHVSRMIA 310 320 330 340 350 360 STQLMNQAPY VKEDTDVTVV IPVASEKDVL PARKLLARQA RLCLFPTEEA RKTRMVVAVF 370 380 390 400 410 420 PLIESRSVTA ITNDMEELKR RCKRSLLETD VLPVHPAVST EGKGTAAAAA LDDAVDRYGA 430 440 450 460 470 480 NTIYLLLSPH ADVQKEFFDR ARINTIKHYQ VFFPVPFVEY HPTISGMEMT EKEEKETPTE 490 500 510 520 530 540 QAREAALSRL RDGVEPKRKR TLIVQKEHGR FDSQDFSCFA VYGVDYVTAR AKFGQNERRN 550 560 570 580 590 600 DLISAFLGQD SIHVLRAVEP TLRIRYHKRS CDMESIDTED IARCLDSKKE NVAAKDQLAK LLFHEK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nematode chondroitin polymerizing factor showing cell-/organ-specific expression is indispensable for chondroitin synthesis and embryonic cell division." Izumikawa T., Kitagawa H., Mizuguchi S., Nomura K.H., Nomura K., Tamura J., Gengyo-Ando K., Mitani S., Sugahara K. J. Biol. Chem. 279:53755-53761(2004) [PubMed: 15485872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A AND B), FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
[2]	"2.2 Mb of contiguous nucleotide sequence from chromosome III of C. elegans." Wilson R., Ainscough R., Anderson K., Baynes C., Berks M., Bonfield J., Burton J., Connell M., Copsey T., Cooper J., Coulson A., Craxton M., Dear S., Du Z., Durbin R., Favello A., Fraser A., Fulton L. , Gardner A., Green P., Hawkins T., Hillier L., Jier M., Johnston L., Jones M., Kershaw J., Kirsten J., Laisster N., Latreille P., Lightning J., Lloyd C., Mortimore B., O'Callaghan M., Parsons J., Percy C., Rifken L., Roopra A., Saunders D., Shownkeen R., Sims M., Smaldon N., Smith A., Smith M., Sonnhammer E., Staden R., Sulston J., Thierry-Mieg J., Thomas K., Vaudin M., Vaughan K., Waterston R., Watson A., Weinstock L., Wilkinson-Sproat J., Wohldman P. Nature 368:32-38(1994) [PubMed: 7906398] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[3]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING. Strain: Bristol N2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB110823 mRNA. Translation: BAD80738.1. AB110824 mRNA. Translation: BAD80739.1. U00025 Genomic DNA. Translation: AAA50619.1. U00025 Genomic DNA. Translation: AAN65312.1.
PIR	S44860.
RefSeq	NP_498934.1. NP_871671.1.
UniGene	Cel.7531
3D structure databases
SMR	P45895. Positions 119-251.
ModBase	Search...
Protein family/group databases
CAZy	GT31. Glycosyltransferase Family 31. GT7. Glycosyltransferase Family 7.
Proteomic databases
PRIDE	P45895.
Genome annotation databases
Ensembl	PAR2.4a.1; PAR2.4a.1; PAR2.4; Caenorhabditis elegans. [Genome view] PAR2.4a.2; PAR2.4a.2; PAR2.4; Caenorhabditis elegans. [Genome view] PAR2.4a.3; PAR2.4a.3; PAR2.4; Caenorhabditis elegans. [Genome view]
GeneID	176231.
KEGG	cel:PAR2.4.
Organism-specific databases
CTD	176231.
WormBase	WBGene00019802. mig-22.
WormPep	PAR2.4a. CE00848. [WorfDB] PAR2.4b. CE32699. [WorfDB]
Phylogenomic databases
eggNOG	meNOG10829.
HOGENOM	HBG382017.
InParanoid	P45895.
OMA	EYHPTIS.
PhylomeDB	P45895.
Enzyme and pathway databases
BRENDA	2.4.1.175. 672. 2.4.1.226. 672.
Gene expression databases
ArrayExpress	P45895.
Family and domain databases
InterPro	IPR008428. Chond_GalNAc. [Graphical view]
PANTHER	PTHR12369. CHGN. 1 hit.
Pfam	PF05679. CHGN. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	891678.

Entry information

Entry name

CHSS2_CAEEL

Accession

Primary (citable) accession number: P45895
Secondary accession number(s): Q5NUN9, Q8IG00

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	February 9, 2010
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project