Reviewed, UniProtKB/Swiss-Prot P45894 (AAPK1_CAEEL)

Names and origin

Protein names

Recommended name:
    5'-AMP-activated protein kinase catalytic subunit alpha-1
      Short name=AMP-activated kinase 1
    EC=2.7.11.1

Gene names

Name:	aak-1
ORF Names:	PAR2.3

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	589 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Probably does not act as a sensor that couples lifespan to information about energy levels and insulin-like signals.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Sequence similarities	Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform a (identifier: P45894-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform b (identifier: P45894-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-171: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 589	589	5'-AMP-activated protein kinase catalytic subunit alpha-1		PRO_0000085598
Regions
	Domain	24 – 276	253	Protein kinase
	Nucleotide binding	30 – 38	9	ATP By similarity
Sites
	Active site	147	1	Proton acceptor By similarity
	Binding site	53	1	ATP By similarity
Natural variations
	Alternative sequence	1 – 171	171	Missing in isoform b.		VSP_017190
Experimental info
	Sequence conflict	205	1	L → P in AAR06927. Ref.1
	Sequence conflict	277	1	R → H in AAR06927. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform a [UniParc]. Last modified February 7, 2006. Version 2. Checksum: AED4E43EFBFDD863	FASTA	589	65,620
	10 20 30 40 50 60 MPPSGRFDRT IALAGTGHLK IGNFVIKETI GKGAFGAVKR GTHIQTGYDV AIKILNRGRM 70 80 90 100 110 120 KGLGTVNKTR NEIDNLQKLT HPHITRLFRV ISTPSDIFLV MELVSGGELF SYITRKGALP 130 140 150 160 170 180 IRESRRYFQQ IISGVSYCHN HMIVHRDLKP ENLLLDANKN IKIADFGLSN YMTDGDLLST 190 200 210 220 230 240 ACGSPNYAAP ELISNKLYVG PEVDLWSCGV ILYAMLCGTL PFDDQNVPTL FAKIKSGRYT 250 260 270 280 290 300 VPYSMEKQAA DLISTMLQVD PVKRADVKRI VNHSWFRIDL PYYLFPECEN ESSIVDIDVV 310 320 330 340 350 360 QSVAEKFDVK EEDVTGALLA EDHHHFLCIA YRLEVNHKRN ADESSQKAME DFWEIGKTMK 370 380 390 400 410 420 MGSTSLPVGA TTKTNVGRKI LEGLKKEQKK LTWNLGIRAC LDPVETMKHV FLSLKSVDME 430 440 450 460 470 480 WKVLSMYHII VRSKPTPINP DPVKVSLQLF ALDKKENNKG YLLDFKGLTE DEEAVPPSRC 490 500 510 520 530 540 RSRAASVSVT LAKSKSDLNG NSSKVPMSPL SPMSPISPSV NIPKVRVDDA DASLKSSLNS 550 560 570 580 SIYMADIENS MESLDEVSTQ SSEPEAPIRS QTMEFFATCH IIMQALLAE
	Isoform b. Checksum: 1DD62D32B61C2CC7	FASTA	418	46,479
	10 20 30 40 50 60 MTDGDLLSTA CGSPNYAAPE LISNKLYVGP EVDLWSCGVI LYAMLCGTLP FDDQNVPTLF 70 80 90 100 110 120 AKIKSGRYTV PYSMEKQAAD LISTMLQVDP VKRADVKRIV NHSWFRIDLP YYLFPECENE 130 140 150 160 170 180 SSIVDIDVVQ SVAEKFDVKE EDVTGALLAE DHHHFLCIAY RLEVNHKRNA DESSQKAMED 190 200 210 220 230 240 FWEIGKTMKM GSTSLPVGAT TKTNVGRKIL EGLKKEQKKL TWNLGIRACL DPVETMKHVF 250 260 270 280 290 300 LSLKSVDMEW KVLSMYHIIV RSKPTPINPD PVKVSLQLFA LDKKENNKGY LLDFKGLTED 310 320 330 340 350 360 EEAVPPSRCR SRAASVSVTL AKSKSDLNGN SSKVPMSPLS PMSPISPSVN IPKVRVDDAD 370 380 390 400 410 ASLKSSLNSS IYMADIENSM ESLDEVSTQS SEPEAPIRSQ TMEFFATCHI IMQALLAE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The AMP-activated protein kinase AAK-2 links energy levels and insulin-like signals to lifespan in C. elegans." Apfeld J., O'Connor G., McDonagh T., DiStefano P.S., Curtis R. Genes Dev. 18:3004-3009(2004) [PubMed: 15574588] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A).
[2]	Erratum Apfeld J., O'Connor G., McDonagh T., DiStefano P.S., Curtis R. Genes Dev. 19:188-188(2005)
[3]	Erratum Apfeld J., O'Connor G., McDonagh T., DiStefano P.S., Curtis R. Genes Dev. 19:411-411(2005)
[4]	"2.2 Mb of contiguous nucleotide sequence from chromosome III of C. elegans." Wilson R., Ainscough R., Anderson K., Baynes C., Berks M., Bonfield J., Burton J., Connell M., Copsey T., Cooper J., Coulson A., Craxton M., Dear S., Du Z., Durbin R., Favello A., Fraser A., Fulton L. , Gardner A., Green P., Hawkins T., Hillier L., Jier M., Johnston L., Jones M., Kershaw J., Kirsten J., Laisster N., Latreille P., Lightning J., Lloyd C., Mortimore B., O'Callaghan M., Parsons J., Percy C., Rifken L., Roopra A., Saunders D., Shownkeen R., Sims M., Smaldon N., Smith A., Smith M., Sonnhammer E., Staden R., Sulston J., Thierry-Mieg J., Thomas K., Vaudin M., Vaughan K., Waterston R., Watson A., Weinstock L., Wilkinson-Sproat J., Wohldman P. Nature 368:32-38(1994) [PubMed: 7906398] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[5]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY347272 mRNA. Translation: AAR06927.1. U00025 Genomic DNA. Translation: ABA54413.1. U00025 Genomic DNA. Translation: ABA54414.1.
PIR	S44859.
RefSeq	NP_741254.3. NP_741255.3.
UniGene	Cel.10244
3D structure databases
HSSP	HSSP built from PDB template 1A06 based on UniProtKB Q63450.
ModBase	Search...
Proteomic databases
PRIDE	P45894.
Genome annotation databases
Ensembl	PAR2.3a; PAR2.3a; PAR2.3; Caenorhabditis elegans. [Genome view] PAR2.3b.1; PAR2.3b.1; PAR2.3; Caenorhabditis elegans. [Genome view] PAR2.3b.2; PAR2.3b.2; PAR2.3; Caenorhabditis elegans. [Genome view]
GeneID	176230.
KEGG	cel:PAR2.3.
UCSC	PAR2.3b.1. c. elegans.
Organism-specific databases
CTD	176230.
WormBase	WBGene00019801. aak-1.
WormPep	PAR2.3a. CE39167. [WorfDB] PAR2.3b. CE39168. [WorfDB]
Phylogenomic databases
OMA	CENESSI.
Enzyme and pathway databases
BRENDA	2.7.11.1. 672.
Gene expression databases
ArrayExpress	P45894.
Family and domain databases
InterPro	IPR015741. AMPK. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
PANTHER	PTHR22982:SF61. AMPK. 1 hit.
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	891672.

Entry information

Entry name

AAPK1_CAEEL

Accession

Primary (citable) accession number: P45894
Secondary accession number(s): Q3HKC8, Q3HKC9, Q5VKL2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	February 7, 2006
Last modified:	November 3, 2009
This is version 64 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project