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Protein

Actin-related protein 2

Gene

Arp2

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Functions as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the pointed end of the daughter actin filament (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi165 – 1673ATPBy similarity
Nucleotide bindingi219 – 2235ATPBy similarity
Nucleotide bindingi310 – 3156ATPBy similarity

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • structural constituent of cytoskeleton Source: FlyBase

GO - Biological processi

  • actin filament organization Source: FlyBase
  • Arp2/3 complex-mediated actin nucleation Source: FlyBase
  • cell morphogenesis Source: FlyBase
  • cytoskeleton organization Source: FlyBase
  • positive regulation of synaptic growth at neuromuscular junction Source: FlyBase
  • regulation of actin polymerization or depolymerization Source: FlyBase
  • regulation of filopodium assembly Source: FlyBase
  • sensory perception of pain Source: FlyBase
  • terminal button organization Source: FlyBase
Complete GO annotation...

Keywords - Ligandi

Actin-binding, ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DME-2029482. Regulation of actin dynamics for phagocytic cup formation.
R-DME-3928662. EPHB-mediated forward signaling.
R-DME-5663213. RHO GTPases Activate WASPs and WAVEs.
SignaLinkiP45888.

Names & Taxonomyi

Protein namesi
Recommended name:
Actin-related protein 2
Alternative name(s):
Actin-like protein 14D
Actin-like protein 2
Gene namesi
Name:Arp2
Synonyms:Actr14D, Arp14D
ORF Names:CG9901
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0011742. Arp2.

Subcellular locationi

GO - Cellular componenti

  • actin cytoskeleton Source: FlyBase
  • actin filament Source: FlyBase
  • Arp2/3 protein complex Source: FlyBase
  • cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 399399Actin-related protein 2PRO_0000089075Add
BLAST

Proteomic databases

PaxDbiP45888.
PeptideAtlasiP45888.
PRIDEiP45888.

Expressioni

Gene expression databases

BgeeiP45888.
ExpressionAtlasiP45888. differential.
GenevisibleiP45888. DM.

Interactioni

Subunit structurei

Component of the Arp2/3 complex.By similarity

Protein-protein interaction databases

BioGridi58961. 11 interactions.
STRINGi7227.FBpp0302793.

Structurei

3D structure databases

ProteinModelPortaliP45888.
SMRiP45888. Positions 4-393.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the actin family. ARP2 subfamily.Curated

Phylogenomic databases

eggNOGiKOG0677. Eukaryota.
COG5277. LUCA.
GeneTreeiENSGT00840000129873.
InParanoidiP45888.
KOiK17260.
OMAiSTYIAIQ.
OrthoDBiEOG78D7K6.

Family and domain databases

InterProiIPR004000. Actin.
IPR020902. Actin/actin-like_CS.
IPR027306. Arp2.
[Graphical view]
PANTHERiPTHR11937. PTHR11937. 1 hit.
PTHR11937:SF149. PTHR11937:SF149. 1 hit.
PfamiPF00022. Actin. 1 hit.
[Graphical view]
PRINTSiPR00190. ACTIN.
SMARTiSM00268. ACTIN. 1 hit.
[Graphical view]
PROSITEiPS01132. ACTINS_ACT_LIKE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform C (identifier: P45888-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDSKGRNVIV CDNGTGFVKC GYAGSNFPTH IFPSMVGRPM IRAVNKIGDI
60 70 80 90 100
EVKDLHVDDL MVGDEASQLR SLLEVSYPME NGVVRNWDDM CHVWDYTFGP
110 120 130 140 150
KKMDIDPTNT KILLTEPPMN PTKNREKMIE VMFEKYGFDS AYIAIQAVLT
160 170 180 190 200
LYAQGLISGV VIDSGDGVTH ICPVYEEFAL PHLTRRLDIA GRDITRYLIK
210 220 230 240 250
LLLLRGYAFN HSADFETVRI MKEKLCYIGY DIEMEQRLAL ETTVLVESYT
260 270 280 290 300
LPDGRVIKVG GERFEAPEAL FQPHLINVEG PGIAELAFNT IQAADIDIRP
310 320 330 340 350
ELYKHIVLSG GSTMYPGLPS RLEREIKQLY LERVLKNDTE KLAKFKIRIE
360 370 380 390
DPPRRKDMVF IGGAVLAEVT KDRDGFWMSK QEYQEQGLKV LQKLQKISH
Note: No experimental confirmation available.
Length:399
Mass (Da):45,282
Last modified:January 22, 2014 - v3
Checksum:i1147D420E047B8D9
GO
Isoform A (identifier: P45888-2) [UniParc]FASTAAdd to basket

Also known as: B

The sequence of this isoform differs from the canonical sequence as follows:
     56-60: Missing.

Show »
Length:394
Mass (Da):44,703
Checksum:i46BE0026B169B9A1
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti2 – 21D → DR in CAA55238 (PubMed:8064864).Curated
Sequence conflicti95 – 951D → E in CAA55238 (PubMed:8064864).Curated
Sequence conflicti148 – 1492VL → AW in CAA55238 (PubMed:8064864).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei56 – 605Missing in isoform A. 2 PublicationsVSP_053563

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X78486 mRNA. Translation: CAA55238.1.
AE014298 Genomic DNA. Translation: AAF48621.1.
AE014298 Genomic DNA. Translation: ABW09432.1.
AE014298 Genomic DNA. Translation: AGB95463.1.
AY061256 mRNA. Translation: AAL28804.1.
BT126051 mRNA. Translation: ADY17750.1.
PIRiS47987.
RefSeqiNP_001097001.1. NM_001103531.2. [P45888-2]
NP_001259621.1. NM_001272692.1. [P45888-1]
NP_523372.2. NM_078648.4. [P45888-2]
UniGeneiDm.183.

Genome annotation databases

EnsemblMetazoaiFBtr0310673; FBpp0302793; FBgn0011742. [P45888-1]
GeneIDi32623.
KEGGidme:Dmel_CG9901.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X78486 mRNA. Translation: CAA55238.1.
AE014298 Genomic DNA. Translation: AAF48621.1.
AE014298 Genomic DNA. Translation: ABW09432.1.
AE014298 Genomic DNA. Translation: AGB95463.1.
AY061256 mRNA. Translation: AAL28804.1.
BT126051 mRNA. Translation: ADY17750.1.
PIRiS47987.
RefSeqiNP_001097001.1. NM_001103531.2. [P45888-2]
NP_001259621.1. NM_001272692.1. [P45888-1]
NP_523372.2. NM_078648.4. [P45888-2]
UniGeneiDm.183.

3D structure databases

ProteinModelPortaliP45888.
SMRiP45888. Positions 4-393.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi58961. 11 interactions.
STRINGi7227.FBpp0302793.

Proteomic databases

PaxDbiP45888.
PeptideAtlasiP45888.
PRIDEiP45888.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0310673; FBpp0302793; FBgn0011742. [P45888-1]
GeneIDi32623.
KEGGidme:Dmel_CG9901.

Organism-specific databases

CTDi32623.
FlyBaseiFBgn0011742. Arp2.

Phylogenomic databases

eggNOGiKOG0677. Eukaryota.
COG5277. LUCA.
GeneTreeiENSGT00840000129873.
InParanoidiP45888.
KOiK17260.
OMAiSTYIAIQ.
OrthoDBiEOG78D7K6.

Enzyme and pathway databases

ReactomeiR-DME-2029482. Regulation of actin dynamics for phagocytic cup formation.
R-DME-3928662. EPHB-mediated forward signaling.
R-DME-5663213. RHO GTPases Activate WASPs and WAVEs.
SignaLinkiP45888.

Miscellaneous databases

GenomeRNAii32623.
PROiP45888.

Gene expression databases

BgeeiP45888.
ExpressionAtlasiP45888. differential.
GenevisibleiP45888. DM.

Family and domain databases

InterProiIPR004000. Actin.
IPR020902. Actin/actin-like_CS.
IPR027306. Arp2.
[Graphical view]
PANTHERiPTHR11937. PTHR11937. 1 hit.
PTHR11937:SF149. PTHR11937:SF149. 1 hit.
PfamiPF00022. Actin. 1 hit.
[Graphical view]
PRINTSiPR00190. ACTIN.
SMARTiSM00268. ACTIN. 1 hit.
[Graphical view]
PROSITEiPS01132. ACTINS_ACT_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genes encoding actin-related proteins of Drosophila melanogaster."
    Fyrberg C., Ryan L., Kenton M., Fyrberg E.A.
    J. Mol. Biol. 241:498-503(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A).
    Strain: Oregon-R.
  2. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  3. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
    Strain: Berkeley.
    Tissue: Embryo.
  5. Carlson J., Booth B., Frise E., Park S., Wan K., Yu C., Celniker S.
    Submitted (FEB-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM C).
    Strain: Berkeley.
    Tissue: Embryo.

Entry informationi

Entry nameiARP2_DROME
AccessioniPrimary (citable) accession number: P45888
Secondary accession number(s): A8JV17, F0JAK9, Q9VXF3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 22, 2014
Last modified: July 6, 2016
This is version 126 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.