Reviewed, UniProtKB/Swiss-Prot P45880 (VDAC2_HUMAN)

Names and origin

Protein names

Recommended name:
    Voltage-dependent anion-selective channel protein 2
      Short name=VDAC-2
      Short name=hVDAC2
Alternative name(s):
    Outer mitochondrial membrane protein porin 2

Gene names

Name:

VDAC2

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	294 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules. The channel adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective.
Subunit structure	Interacts with hexokinases By similarity.
Subcellular location	Mitochondrion outer membrane. Ref.9
Tissue specificity	Expressed in all tissues examined.
Domain	Consists mainly of a membrane-spanning beta-barrel formed by 19 beta-strands By similarity.
Sequence similarities	Belongs to the eukaryotic mitochondrial porin family.
Sequence caution	The sequence CAH73106.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAI40910.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Membrane Mitochondrion Mitochondrion outer membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Transmembrane
Ligand	NAD Nucleotide-binding
Molecular function	Porin
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	anion transport Ref.2 Traceable author statement. Source: ProtInc
Cellular component	mitochondrial nucleoid Inferred from direct assay. Source: UniProtKB mitochondrial outer membrane Ref.2 Traceable author statement. Source: ProtInc pore complex Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct voltage-gated anion channel activity Ref.2 Traceable author statement. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
BAK1	Q16611	1	EBI-354022,EBI-519866
SERINC3	Q13530	1	EBI-354022,EBI-1045571

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 3 (identifier: P45880-3)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 1 (identifier: P45880-1)

The sequence of this isoform differs from the canonical sequence as follows:
     1-11: MATHGQTCARP → MSWCNELRLPALKQHSIGRGLESHIT

Note: Ref.1 (AAA60144) sequence differs from that shown due to a frameshift in position 303.

Isoform 2 (identifier: P45880-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-11: Missing.

Note: Ref.1 (AAA60145) sequence differs from that shown due to a frameshift in position 277.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 294	294	Voltage-dependent anion-selective channel protein 2		PRO_0000050505
Regions
	Transmembrane	37 – 46	10	Transmembrane beta-strand By similarity
	Transmembrane	50 – 58	9	Transmembrane beta-strand By similarity
	Transmembrane	65 – 75	11	Transmembrane beta-strand By similarity
	Transmembrane	80 – 87	8	Transmembrane beta-strand By similarity
	Transmembrane	91 – 100	10	Transmembrane beta-strand By similarity
	Transmembrane	106 – 115	10	Transmembrane beta-strand By similarity
	Transmembrane	122 – 131	10	Transmembrane beta-strand By similarity
	Transmembrane	134 – 141	8	Transmembrane beta-strand By similarity
	Transmembrane	148 – 156	9	Transmembrane beta-strand By similarity
	Transmembrane	161 – 169	9	Transmembrane beta-strand By similarity
	Transmembrane	174 – 186	13	Transmembrane beta-strand By similarity
	Transmembrane	189 – 196	8	Transmembrane beta-strand By similarity
	Transmembrane	200 – 209	10	Transmembrane beta-strand By similarity
	Transmembrane	213 – 222	10	Transmembrane beta-strand By similarity
	Transmembrane	229 – 238	10	Transmembrane beta-strand By similarity
	Transmembrane	242 – 249	8	Transmembrane beta-strand By similarity
	Transmembrane	253 – 262	10	Transmembrane beta-strand By similarity
	Transmembrane	265 – 274	10	Transmembrane beta-strand By similarity
	Transmembrane	284 – 293	10	Transmembrane beta-strand By similarity
	Nucleotide binding	253 – 255	3	NAD By similarity
	Nucleotide binding	271 – 275	5	NAD By similarity
Sites
	Site	84	1	Involved in hexokinase binding By similarity
Amino acid modifications
	Modified residue	31	1	N6-acetyllysine Ref.14
	Modified residue	39	1	N6-acetyllysine Ref.14
	Modified residue	72	1	N6-acetyllysine Ref.14
	Modified residue	74	1	N6-acetyllysine Ref.14
	Modified residue	115	1	Phosphoserine Ref.10 Ref.11 Ref.12 Ref.13
	Modified residue	206	1	Phosphotyrosine By similarity
	Modified residue	236	1	Phosphotyrosine By similarity
Natural variations
	Alternative sequence	1 – 11	11	MATHGQTCARP → MSWCNELRLPALKQHSIGRG LESHIT in isoform 1.		VSP_005077
	Alternative sequence	1 – 11	11	Missing in isoform 2.		VSP_005076
	Natural variant	24	1	A → V		VAR_006380

Sequences

	Sequence		Length	Mass (Da)
	Isoform 3 [UniParc]. Last modified September 11, 2007. Version 2. Checksum: F4EAE732E653637E	FASTA	294	31,567
	10 20 30 40 50 60 MATHGQTCAR PMCIPPSYAD LGKAARDIFN KGFGFGLVKL DVKTKSCSGV EFSTSGSSNT 70 80 90 100 110 120 DTGKVTGTLE TKYKWCEYGL TFTEKWNTDN TLGTEIAIED QICQGLKLTF DTTFSPNTGK 130 140 150 160 170 180 KSGKIKSSYK RECINLGCDV DFDFAGPAIH GSAVFGYEGW LAGYQMTFDS AKSKLTRNNF 190 200 210 220 230 240 AVGYRTGDFQ LHTNVNDGTE FGGSIYQKVC EDLDTSVNLA WTSGTNCTRF GIAAKYQLDP 250 260 270 280 290 TASISAKVNN SSLIGVGYTQ TLRPGVKLTL SALVDGKSIN AGGHKVGLAL ELEA
	Isoform 1. Checksum: 7A94C18F1C07DEBA	FASTA	309	33,372
	10 20 30 40 50 60 MSWCNELRLP ALKQHSIGRG LESHITMCIP PSYADLGKAA RDIFNKGFGF GLVKLDVKTK 70 80 90 100 110 120 SCSGVEFSTS GSSNTDTGKV TGTLETKYKW CEYGLTFTEK WNTDNTLGTE IAIEDQICQG 130 140 150 160 170 180 LKLTFDTTFS PNTGKKSGKI KSSYKRECIN LGCDVDFDFA GPAIHGSAVF GYEGWLAGYQ 190 200 210 220 230 240 MTFDSAKSKL TRNNFAVGYR TGDFQLHTNV NDGTEFGGSI YQKVCEDLDT SVNLAWTSGT 250 260 270 280 290 300 NCTRFGIAAK YQLDPTASIS AKVNNSSLIG VGYTQTLRPG VKLTLSALVD GKSINAGGHK VGLALELEA
	Isoform 2. Checksum: 9A66CDF9CF0542B9	FASTA	283	30,412
	10 20 30 40 50 60 MCIPPSYADL GKAARDIFNK GFGFGLVKLD VKTKSCSGVE FSTSGSSNTD TGKVTGTLET 70 80 90 100 110 120 KYKWCEYGLT FTEKWNTDNT LGTEIAIEDQ ICQGLKLTFD TTFSPNTGKK SGKIKSSYKR 130 140 150 160 170 180 ECINLGCDVD FDFAGPAIHG SAVFGYEGWL AGYQMTFDSA KSKLTRNNFA VGYRTGDFQL 190 200 210 220 230 240 HTNVNDGTEF GGSIYQKVCE DLDTSVNLAW TSGTNCTRFG IAAKYQLDPT ASISAKVNNS 250 260 270 280 SLIGVGYTQT LRPGVKLTLS ALVDGKSINA GGHKVGLALE LEA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A mitochondrial porin cDNA predicts the existence of multiple human porins." Ha H., Hajek P., Bedwell D.M., Burrows P.D. J. Biol. Chem. 268:12143-12149(1993) [PubMed: 7685033] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2). Tissue: B-cell.
[2]	"Cloning and functional expression in yeast of two human isoforms of the outer mitochondrial membrane channel, the voltage-dependent anion channel." Blachly-Dyson E., Zambronicz E.B., Yu W.H., Adams V., McCabe E.R., Adelman J.P., Colombini M., Forte M.A. J. Biol. Chem. 268:1835-1841(1993) [PubMed: 8420959] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), VARIANT VAL-24. Tissue: Liver.
[3]	"Revised fine mapping of the human voltage-dependent anion channel loci by radiation hybrid analysis." Decker W.K., Bowles K.R., Schatte E.C., Towbin J.A., Craigen W.J. Mamm. Genome 10:1041-1042(1999) [PubMed: 10501981] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 3).
[4]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[5]	"The DNA sequence and comparative analysis of human chromosome 10." Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J. , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J. Nature 429:375-381(2004) [PubMed: 15164054] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Cervix and Eye.
[7]	Lubec G., Vishwanath V., Chen W.-Q., Sun Y. Submitted (DEC-2008) to UniProtKB Cited for: PROTEIN SEQUENCE OF 86-120; 178-229 AND 248-267, MASS SPECTROMETRY. Tissue: Brain, Cajal-Retzius cell and Fetal brain cortex.
[8]	Bienvenut W.V. Submitted (JUN-2005) to UniProtKB Cited for: PROTEIN SEQUENCE OF 11-23 (ISOFORM 3), PROTEIN SEQUENCE OF 46-64; 75-85; 108-120; 178-185; 236-263 AND 268-277 (ISOFORMS 1/2/3), MASS SPECTROMETRY. Tissue: B-cell lymphoma.
[9]	"Subcellular localization of human voltage-dependent anion channel isoforms." Yu W.H., Wolfgang W., Forte M.A. J. Biol. Chem. 270:13998-14006(1995) [PubMed: 7539795] [Abstract] Cited for: SUBCELLULAR LOCATION.
[10]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-115, MASS SPECTROMETRY. Tissue: Epithelium.
[11]	"Phosphoproteome of resting human platelets." Zahedi R.P., Lewandrowski U., Wiesner J., Wortelkamp S., Moebius J., Schuetz C., Walter U., Gambaryan S., Sickmann A. J. Proteome Res. 7:526-534(2008) [PubMed: 18088087] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-115, MASS SPECTROMETRY. Tissue: Platelet.
[12]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-115, MASS SPECTROMETRY.
[13]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-115, MASS SPECTROMETRY.
[14]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed: 19608861] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-31; LYS-39; LYS-72 AND LYS-74, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L08666 mRNA. Translation: AAA60144.1. Frameshift. L08666 mRNA. Translation: AAA60145.1. Frameshift. L06328 mRNA. Translation: AAB59457.1. AF152227 , AF152220, AF152221, AF152222, AF152223, AF152224, AF152225, AF152226 Genomic DNA. Translation: AAD40241.1. CR456964 mRNA. Translation: CAG33245.1. AL390034, AL392111 Genomic DNA. Translation: CAH73108.1. AL392111, AL390034 Genomic DNA. Translation: CAI40914.1. AL390034, AL392111 Genomic DNA. Translation: CAH73107.1. AL392111, AL390034 Genomic DNA. Translation: CAI40911.1. AL390034, AL392111 Genomic DNA. Translation: CAH73106.1. Sequence problems. AL392111, AL390034 Genomic DNA. Translation: CAI40910.1. Sequence problems. BC000165 mRNA. Translation: AAH00165.2. BC012883 mRNA. Translation: AAH12883.2. BC072407 mRNA. Translation: AAH72407.1.
IPI	IPI00024145. IPI00216026. IPI00902560.
PIR	A45972. B44422.
RefSeq	NP_003366.2.
UniGene	Hs.355927
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P45880. 6 interactions.
STRING	P45880.
PTM databases
PhosphoSite	P45880.
2-D gel databases
SWISS-2DPAGE	P45880.
DOSAC-COBS-2DPAGE	P45880.
OGP	P45880.
REPRODUCTION-2DPAGE	P45880.
Proteomic databases
PRIDE	P45880.
Genome annotation databases
Ensembl	ENST00000298468; ENSP00000298468; ENSG00000165637; Homo sapiens. [Genome view] ENST00000313132; ENSP00000361635; ENSG00000165637; Homo sapiens. [Genome view] ENST00000332211; ENSP00000361686; ENSG00000165637; Homo sapiens. [Genome view] ENST00000344036; ENSP00000344876; ENSG00000165637; Homo sapiens. [Genome view] ENST00000413289; ENSP00000389551; ENSG00000165637; Homo sapiens. [Genome view] ENST00000447677; ENSP00000401492; ENSG00000165637; Homo sapiens. [Genome view]
GeneID	7417.
KEGG	hsa:7417.
UCSC	uc001jwz.1. human. uc001jxa.1. human.
Organism-specific databases
CTD	7417.
GeneCards	GC10P076640.
H-InvDB	HIX0008945. HIX0029994.
HGNC	HGNC:12672. VDAC2.
MIM	193245. gene.
PharmGKB	PA37295.
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	P45880.
Gene expression databases
ArrayExpress	P45880.
Bgee	P45880.
CleanEx	HS_VDAC2.
Genevestigator	P45880.
GermOnline	ENSG00000165637. Homo sapiens.
Family and domain databases
InterPro	IPR001925. Porin_Euk. [Graphical view]
Pfam	PF01459. Porin_3. 1 hit. [Graphical view]
PRINTS	PR00185. EUKARYTPORIN.
PROSITE	PS00558. EUKARYOTIC_PORIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
DrugBank	DB01375. Dihydroxyaluminium.
NextBio	29042.
SOURCE	Search...

Entry information

Entry name

VDAC2_HUMAN

Accession

Primary (citable) accession number: P45880
Secondary accession number(s): Q5VWK1 , Q5VWK3, Q6IB40, Q7L3J5, Q9BWK8, Q9Y5I6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	September 11, 2007
Last modified:	October 13, 2009
This is version 94 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.