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P45880 (VDAC2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 134. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Voltage-dependent anion-selective channel protein 2

Short name=VDAC-2
Short name=hVDAC2
Alternative name(s):
Outer mitochondrial membrane protein porin 2
Gene names
Name:VDAC2
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length294 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules. The channel adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective.

Subunit structure

Interacts with hexokinases By similarity.

Subcellular location

Mitochondrion outer membrane Ref.9.

Tissue specificity

Expressed in all tissues examined.

Domain

Consists mainly of a membrane-spanning beta-barrel formed by 19 beta-strands By similarity.

Sequence similarities

Belongs to the eukaryotic mitochondrial porin family.

Sequence caution

The sequence CAH73106.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence CAI40910.1 differs from that shown. Reason: Erroneous gene model prediction.

Isoform 1: The sequence AAA60144.1 differs from that shown. Reason: Frameshift at position 303.

Isoform 2: The sequence AAA60145.1 differs from that shown. Reason: Frameshift at position 277.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

FAM162AQ96A262EBI-354022,EBI-6123466

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 3 (identifier: P45880-3)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 1 (identifier: P45880-1)

The sequence of this isoform differs from the canonical sequence as follows:
     1-11: MATHGQTCARP → MSWCNELRLPALKQHSIGRGLESHIT
Isoform 2 (identifier: P45880-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-11: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.14
Chain2 – 294293Voltage-dependent anion-selective channel protein 2
PRO_0000050505

Regions

Transmembrane37 – 4610Beta stranded; By similarity
Transmembrane50 – 589Beta stranded; By similarity
Transmembrane65 – 7511Beta stranded; By similarity
Transmembrane80 – 878Beta stranded; By similarity
Transmembrane91 – 10010Beta stranded; By similarity
Transmembrane106 – 11510Beta stranded; By similarity
Transmembrane122 – 13110Beta stranded; By similarity
Transmembrane134 – 1418Beta stranded; By similarity
Transmembrane148 – 1569Beta stranded; By similarity
Transmembrane161 – 1699Beta stranded; By similarity
Transmembrane174 – 18613Beta stranded; By similarity
Transmembrane189 – 1968Beta stranded; By similarity
Transmembrane200 – 20910Beta stranded; By similarity
Transmembrane213 – 22210Beta stranded; By similarity
Transmembrane229 – 23810Beta stranded; By similarity
Transmembrane242 – 2498Beta stranded; By similarity
Transmembrane253 – 26210Beta stranded; By similarity
Transmembrane265 – 27410Beta stranded; By similarity
Transmembrane284 – 29310Beta stranded; By similarity
Nucleotide binding253 – 2553NAD By similarity
Nucleotide binding271 – 2755NAD By similarity

Sites

Site841Involved in hexokinase binding By similarity

Amino acid modifications

Modified residue21N-acetylalanine Ref.14
Modified residue311N6-acetyllysine; alternate Ref.15
Modified residue311N6-succinyllysine; alternate By similarity
Modified residue1201N6-acetyllysine By similarity

Natural variations

Alternative sequence1 – 1111MATHGQTCARP → MSWCNELRLPALKQHSIGRG LESHIT in isoform 1.
VSP_005077
Alternative sequence1 – 1111Missing in isoform 2.
VSP_005076
Natural variant241A → V. Ref.2
VAR_006380

Sequences

Sequence LengthMass (Da)Tools
Isoform 3 [UniParc].

Last modified September 11, 2007. Version 2.
Checksum: F4EAE732E653637E

FASTA29431,567
        10         20         30         40         50         60 
MATHGQTCAR PMCIPPSYAD LGKAARDIFN KGFGFGLVKL DVKTKSCSGV EFSTSGSSNT 

        70         80         90        100        110        120 
DTGKVTGTLE TKYKWCEYGL TFTEKWNTDN TLGTEIAIED QICQGLKLTF DTTFSPNTGK 

       130        140        150        160        170        180 
KSGKIKSSYK RECINLGCDV DFDFAGPAIH GSAVFGYEGW LAGYQMTFDS AKSKLTRNNF 

       190        200        210        220        230        240 
AVGYRTGDFQ LHTNVNDGTE FGGSIYQKVC EDLDTSVNLA WTSGTNCTRF GIAAKYQLDP 

       250        260        270        280        290 
TASISAKVNN SSLIGVGYTQ TLRPGVKLTL SALVDGKSIN AGGHKVGLAL ELEA 

« Hide

Isoform 1 [UniParc].

Checksum: 7A94C18F1C07DEBA
Show »

FASTA30933,372
Isoform 2 [UniParc].

Checksum: 9A66CDF9CF0542B9
Show »

FASTA28330,412

References

« Hide 'large scale' references
[1]"A mitochondrial porin cDNA predicts the existence of multiple human porins."
Ha H., Hajek P., Bedwell D.M., Burrows P.D.
J. Biol. Chem. 268:12143-12149(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
Tissue: B-cell.
[2]"Cloning and functional expression in yeast of two human isoforms of the outer mitochondrial membrane channel, the voltage-dependent anion channel."
Blachly-Dyson E., Zambronicz E.B., Yu W.H., Adams V., McCabe E.R., Adelman J.P., Colombini M., Forte M.A.
J. Biol. Chem. 268:1835-1841(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), VARIANT VAL-24.
Tissue: Liver.
[3]"Revised fine mapping of the human voltage-dependent anion channel loci by radiation hybrid analysis."
Decker W.K., Bowles K.R., Schatte E.C., Towbin J.A., Craigen W.J.
Mamm. Genome 10:1041-1042(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 3).
[4]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[5]"The DNA sequence and comparative analysis of human chromosome 10."
Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J. expand/collapse author list , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J.
Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
Tissue: Cervix and Eye.
[7]Lubec G., Vishwanath V., Chen W.-Q., Sun Y.
Submitted (DEC-2008) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 86-120; 178-229 AND 248-267, IDENTIFICATION BY IDENTIFICATION BY MASS SPECTROMETRY.
Tissue: Brain, Cajal-Retzius cell and Fetal brain cortex.
[8]Bienvenut W.V.
Submitted (JUN-2005) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 11-23 (ISOFORM 3), PROTEIN SEQUENCE OF 46-64; 75-85; 108-120; 178-185; 236-263 AND 268-277 (ISOFORMS 1/2/3), IDENTIFICATION BY MASS SPECTROMETRY.
Tissue: B-cell lymphoma.
[9]"Subcellular localization of human voltage-dependent anion channel isoforms."
Yu W.H., Wolfgang W., Forte M.A.
J. Biol. Chem. 270:13998-14006(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[10]"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[11]"Phosphoproteome of resting human platelets."
Zahedi R.P., Lewandrowski U., Wiesner J., Wortelkamp S., Moebius J., Schuetz C., Walter U., Gambaryan S., Sickmann A.
J. Proteome Res. 7:526-534(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Platelet.
[12]"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[13]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[14]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
[15]"Lysine acetylation targets protein complexes and co-regulates major cellular functions."
Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.
Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-31, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[16]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[17]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[18]"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L08666 mRNA. Translation: AAA60144.1. Frameshift.
L08666 mRNA. Translation: AAA60145.1. Frameshift.
L06328 mRNA. Translation: AAB59457.1.
AF152227 expand/collapse EMBL AC list , AF152220, AF152221, AF152222, AF152223, AF152224, AF152225, AF152226 Genomic DNA. Translation: AAD40241.1.
CR456964 mRNA. Translation: CAG33245.1.
AL390034, AL392111 Genomic DNA. Translation: CAH73108.1.
AL392111, AL390034 Genomic DNA. Translation: CAI40914.1.
AL390034, AL392111 Genomic DNA. Translation: CAH73107.1.
AL392111, AL390034 Genomic DNA. Translation: CAI40911.1.
AL390034, AL392111 Genomic DNA. Translation: CAH73106.1. Sequence problems.
AL392111, AL390034 Genomic DNA. Translation: CAI40910.1. Sequence problems.
BC000165 mRNA. Translation: AAH00165.2.
BC012883 mRNA. Translation: AAH12883.2.
BC072407 mRNA. Translation: AAH72407.1.
PIRA45972.
B44422.
RefSeqNP_001171712.1. NM_001184783.1.
NP_001171752.1. NM_001184823.1.
NP_003366.2. NM_003375.3.
UniGeneHs.355927.

3D structure databases

ProteinModelPortalP45880.
SMRP45880. Positions 12-294.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid113260. 52 interactions.
IntActP45880. 17 interactions.
MINTMINT-3016132.
STRING9606.ENSP00000361686.

Chemistry

ChEMBLCHEMBL6190.
DrugBankDB01375. Dihydroxyaluminium.

PTM databases

PhosphoSiteP45880.

Polymorphism databases

DMDM158518391.

2D gel databases

DOSAC-COBS-2DPAGEP45880.
OGPP45880.
REPRODUCTION-2DPAGEP45880.
SWISS-2DPAGEP45880.
UCD-2DPAGEP45880.

Proteomic databases

PaxDbP45880.
PRIDEP45880.

Protocols and materials databases

DNASU7417.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000313132; ENSP00000361635; ENSG00000165637. [P45880-1]
ENST00000332211; ENSP00000361686; ENSG00000165637. [P45880-3]
ENST00000543351; ENSP00000443092; ENSG00000165637. [P45880-3]
GeneID7417.
KEGGhsa:7417.
UCSCuc001jwz.3. human. [P45880-3]
uc001jxa.3. human. [P45880-1]

Organism-specific databases

CTD7417.
GeneCardsGC10P076969.
H-InvDBHIX0029994.
HGNCHGNC:12672. VDAC2.
HPAHPA043475.
MIM193245. gene.
neXtProtNX_P45880.
PharmGKBPA37295.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG243169.
HOVERGENHBG054036.
KOK15040.
OMAMCVPPSY.
OrthoDBEOG7P8P8G.
PhylomeDBP45880.
TreeFamTF315091.

Gene expression databases

BgeeP45880.
CleanExHS_VDAC2.
GenevestigatorP45880.

Family and domain databases

Gene3D2.40.160.10. 1 hit.
InterProIPR023614. Porin_dom.
IPR001925. Porin_Euk.
IPR027246. Porin_Euk/Tom40.
[Graphical view]
PfamPF01459. Porin_3. 1 hit.
[Graphical view]
PRINTSPR00185. EUKARYTPORIN.
PROSITEPS00558. EUKARYOTIC_PORIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiVDAC2.
GenomeRNAi7417.
NextBio29042.
PROP45880.
SOURCESearch...

Entry information

Entry nameVDAC2_HUMAN
AccessionPrimary (citable) accession number: P45880
Secondary accession number(s): Q5VWK1 expand/collapse secondary AC list , Q5VWK3, Q6IB40, Q7L3J5, Q9BWK8, Q9Y5I6
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: September 11, 2007
Last modified: April 16, 2014
This is version 134 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 10

Human chromosome 10: entries, gene names and cross-references to MIM