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P45868 (MAO2_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable NAD-dependent malic enzyme 2
Short name=NAD-ME 2
EC=1.1.1.38
Gene names
Name:maeA
Ordered Locus Names:BSU37050
OrganismBacillus subtilis
Taxonomic identifier1423 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length582 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

(S)-malate + NAD+ = pyruvate + CO2 + NADH.

Cofactor

Divalent metal cations. Prefers magnesium or manganese By similarity.

Enzyme regulation

Activated by the two-component system malK/malR in response to malate. The regulator malR binds to the promoter region of maeA. Is not subject to carbon catabolite repression.

Sequence similarities

Belongs to the malic enzymes family.

Biophysicochemical properties

Kinetic parameters:

KM=6.6 mM for malate

KM=1.1 mM for NAD

KM=9.0 mM for NADP

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 582582Probable NAD-dependent malic enzyme 2
PRO_0000160209

Sites

Active site1221Proton donor By similarity
Active site1951Proton acceptor By similarity
Metal binding2661Divalent metal cation By similarity
Metal binding2671Divalent metal cation By similarity
Metal binding2901Divalent metal cation By similarity
Binding site2901NAD By similarity
Binding site4391NAD By similarity
Site2901Important for activity By similarity

Sequences

Sequence LengthMass (Da)Tools
P45868 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: E02821E341C20302

FASTA58264,103
        10         20         30         40         50         60 
MGYYLTWLTI SRKKEICLNN IKKTKEGHLE TTLRGKEVLS IPTLNKGVAF SLEERQELGL 

        70         80         90        100        110        120 
EGLLPPTVLS LDQQAQRAYE QFQAQPDRLR QNVYLSDLAN RNEVLFYKLL KNHLREMLPV 

       130        140        150        160        170        180 
VYTPTVGEAI QEYSHEYRRP QGIYLSIDNI DGIEKAFENL HATAGDIDLI VATDSESILG 

       190        200        210        220        230        240 
IGDWGVGGIN IAIGKLAVYT AAAGIDPSRV IPVVLDVGTN NEKLLNDPLY IGNKHERVQG 

       250        260        270        280        290        300 
ERYEAFIDAY VKAALKFFPK ALLHWEDLGN KNARNIMKKY NHEILTFNDD IQGTGAITLA 

       310        320        330        340        350        360 
GVLAAMKKTG ASIKDQRVVI FGAGSAGIGI ADQIRDTMVL AGLSEEEANK RFYTLDYRGL 

       370        380        390        400        410        420 
LTEDIEGILD FQKPYLRNAD EVKDWKRDEK GQIPFDEVVR QAKPTILIGT SGVSGAFTEE 

       430        440        450        460        470        480 
IVKEMASHVD RPVIMPMSNP THLAEAVPED LFKWTDGKVL IATGSPFDNV EYNGVSYEIG 

       490        500        510        520        530        540 
QSNNAFAFPG LGLGSIVAEA RIITPAMFAA TADAIAEMVD LETPGAGLLP SIDKLQEVSI 

       550        560        570        580 
QVAIAVAEAA IKDGVANRQP EDVKQAVLDA MWTPEYKKVI AK

« Hide

References

« Hide 'large scale' references
[1]"The Bacillus subtilis genome from gerBC (311 degrees) to licR (334 degrees)."
Presecan E., Moszer I., Boursier L., Cruz Ramos H., De La Fuente V., Hullo M.-F., Lelong C., Schleich S., Sekowska A., Song B.H., Villani G., Kunst F., Danchin A., Glaser P.
Microbiology 143:3313-3328(1997) [PubMed: 9353933] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[2]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed: 9384377] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[3]"The Bacillus subtilis ywkA gene encodes a malic enzyme and its transcription is activated by the YufL/YufM two-component system in response to malate."
Doan T., Servant P., Tojo S., Yamaguchi H., Lerondel G., Yoshida K., Fujita Y., Aymerich S.
Microbiology 149:2331-2343(2003) [PubMed: 12949160] [Abstract]
Cited for: FUNCTION, CHARACTERIZATION, REGULATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		Z49782 Genomic DNA. Translation: CAA89880.1.
AL009126 Genomic DNA. Translation: CAB15722.1.
PIRS55433.
RefSeqNP_391586.1. NC_000964.3.

3D structure databases

ProteinModelPortalP45868.
SMRP45868. Positions 15-581.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBBACT00000003481; EBBACP00000003481; EBBACG00000003474.
GeneID937045.
GenomeReviewsGene locus BSU37050 in contig AL009126_GR.
KEGGbsu:BSU37050.
NMPDRfig|224308.1.peg.3712.
PATRIC18979444. VBIBacSub10457_3884.

Organism-specific databases

GenoListBSU37050. [Micado]

Phylogenomic databases

GeneTreeEBGT00050000001535.
HOGENOMHBG289821.
OMANARNIMK.
PhylomeDBP45868.
ProtClustDBPRK13529.

Enzyme and pathway databases

BioCycBSUB:BSU37050-MONOMER.

Family and domain databases

InterProIPR015884. Malic_enzyme_CS.
IPR012301. Malic_N.
IPR012302. Malic_NAD-bd.
IPR001891. Malic_OxRdtase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
Gene3DG3DSA:3.40.50.10380. G3DSA:3.40.50.10380. 1 hit.
G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KOK00027.
PfamPF00390. malic. 1 hit.
PF03949. Malic_M. 1 hit.
[Graphical view]
PIRSFPIRSF000106. ME. 1 hit.
PRINTSPR00072. MALOXRDTASE.
SMARTSM00919. Malic_M. 1 hit.
[Graphical view]
PROSITEPS00331. MALIC_ENZYMES. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMAO2_BACSU
AccessionPrimary (citable) accession number: P45868
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: January 25, 2012
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents