Reviewed, UniProtKB/Swiss-Prot P45859 (PRPD_BACSU)

Names and origin

Protein names

Recommended name:
    2-methylcitrate dehydratase
    EC=4.2.1.79

Gene names

Name:	prpD
Synonyms:	mmgE, yqiP
Ordered Locus Names:	BSU24130

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	472 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the dehydration of 2-methylcitrate to 2-methyl-cis-aconitate By similarity.
Catalytic activity	(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate = (Z)-but-2-ene-1,2,3-tricarboxylate + H2O.
Cofactor	Binds 1 2Fe-2S cluster By similarity.
Pathway	Organic acid metabolism; propanoate degradation.
Developmental stage	Expressed in the mother cell at intermediate stages of sporulation under the control of the sigma-E factor. Ref.4
Induction	Subject to catabolite repression. Ref.4
Sequence similarities	Belongs to the prpD family.

Ontologies

Keywords
Biological process	Sporulation
Ligand	2Fe-2S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	propionate catabolic process, 2-methylcitrate cycle Inferred from electronic annotation. Source: InterPro sporulation resulting in formation of a cellular spore Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 2-methylcitrate dehydratase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 472	472	2-methylcitrate dehydratase		PRO_0000215020
Experimental info
	Sequence conflict	216	1	A → P in BAA12591. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P45859-1 [UniParc]. Last modified July 7, 2009. Version 3. Checksum: 5941EA6EC1DA2142	FASTA	472	52,878
	10 20 30 40 50 60 MPKTDRVIEE ITDYVLEKEI TSAEAYTTAG HVLLDTLGCG ILALRYPECT KLLGPIVPGT 70 80 90 100 110 120 TVPNGSKVPG TSYVLDPVRA AFNIGCMIRW LDYNDTWLAA EWGHPSDNLG GILAAADYVS 130 140 150 160 170 180 RVRLSEGKEP LTVRDVLEMM IKAHEIQGVL ALENSLNRVG LDHVLFVKVA TTAVAAKLLG 190 200 210 220 230 240 GGREEIKNAL SNAWIDNAAL RTYRHSPNTG SRKSWAAGDA TSRGVHLALM SLKGEMGYPT 250 260 270 280 290 300 ALSAPGWGFQ DVLFNKKEIK LARPLDAYVM ENVLFKVSYP AEFHAQTAAE SAVILHPQVK 310 320 330 340 350 360 NRIDEIDRVV IRTHESAIRI IDKKGPLHNP ADRDHCLQYI TAIGLLFGDI TAQHYEAETA 370 380 390 400 410 420 NDPRIDKLRD KMEVTENKTY TEDYLKPDKR SISNAVQVHF KDGTSTEMVE CEFPLGHRFR 430 440 450 460 470 REEAVPKLLE KFSDNLKTHF PDKQHKHIYE RCTSYETLQT MRVNEFVDMF CM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the Bacillus subtilis genome containing the skin element and many sporulation genes." Mizuno M., Masuda S., Takemaru K., Hosono S., Sato T., Takeuchi M., Kobayashi Y. Microbiology 142:3103-3111(1996) [PubMed: 8969508] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168 / JH642.
[2]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[3]	"From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later." Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A. Microbiology 155:1758-1775(2009) [PubMed: 19383706] [Abstract] Cited for: SEQUENCE REVISION TO 216.
[4]	"A sigma E dependent operon subject to catabolite repression during sporulation in Bacillus subtilis." Bryan E.M., Beall B.W., Moran C.P. Jr. J. Bacteriol. 178:4778-4786(1996) [PubMed: 8759838] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-178, DEVELOPMENTAL STAGE, INDUCTION. Strain: 168 / MB24.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D84432 Genomic DNA. Translation: BAA12591.1. AL009126 Genomic DNA. Translation: CAB14344.2. U29084 Genomic DNA. Translation: AAB09617.1.
PIR	F69658.
RefSeq	NP_390293.2.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	938663.
GenomeReviews	Gene locus BSU24130 in contig AL009126_GR.
KEGG	bsu:BSU24130.
NMPDR	fig|224308.1.peg.2417.
Organism-specific databases
SubtiList	BG11323. prpD. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	P45859.
OMA	ALRFPEC.
Enzyme and pathway databases
BioCyc	BSUB224308:BSU2411-MON.
Family and domain databases
InterPro	IPR012705. 2Me_IsoCit_deHydtase_PrpD. IPR005656. MmgE_PrpD. [Graphical view]
PANTHER	PTHR16943. MmgE_PrpD. 1 hit.
Pfam	PF03972. MmgE_PrpD. 1 hit. [Graphical view]
TIGRFAMs	TIGR02330. prpD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PRPD_BACSU

Accession

Primary (citable) accession number: P45859

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	July 7, 2009
Last modified:	November 3, 2009
This is version 64 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)