P45859 (PRPD_BACSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-methylcitrate dehydratase
EC=4.2.1.79

Gene names

Name:	prpD
Synonyms:	mmgE, yqiP
Ordered Locus Names:	BSU24130

Organism

Bacillus subtilis (strain 168) [Reference proteome] [HAMAP]

Taxonomic identifier

224308 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus subtilis group › Bacillus subtilis › Bacillus subtilis subsp. subtilis

Protein attributes

Sequence length	472 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the dehydration of 2-methylcitrate to 2-methyl-cis-aconitate By similarity.
Catalytic activity	(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate = (Z)-but-2-ene-1,2,3-tricarboxylate + H2O.
Cofactor	Binds 1 2Fe-2S cluster By similarity.
Pathway	Organic acid metabolism; propanoate degradation.
Developmental stage	Expressed in the mother cell at intermediate stages of sporulation under the control of the sigma-E factor. Ref.4
Induction	Subject to catabolite repression. Ref.4
Sequence similarities	Belongs to the PrpD family.

Ontologies

Keywords
Biological process	Sporulation
Ligand	2Fe-2S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	propionate catabolic process, 2-methylcitrate cycle Inferred from electronic annotation. Source: InterPro sporulation resulting in formation of a cellular spore Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 2-methylcitrate dehydratase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 472	472	2-methylcitrate dehydratase		PRO_0000215020
Experimental info
	Sequence conflict	216	1	A → P in BAA12591. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P45859 [UniParc]. Last modified July 7, 2009. Version 3. Checksum: 5941EA6EC1DA2142	FASTA	472	52,878
	10 20 30 40 50 60 MPKTDRVIEE ITDYVLEKEI TSAEAYTTAG HVLLDTLGCG ILALRYPECT KLLGPIVPGT 70 80 90 100 110 120 TVPNGSKVPG TSYVLDPVRA AFNIGCMIRW LDYNDTWLAA EWGHPSDNLG GILAAADYVS 130 140 150 160 170 180 RVRLSEGKEP LTVRDVLEMM IKAHEIQGVL ALENSLNRVG LDHVLFVKVA TTAVAAKLLG 190 200 210 220 230 240 GGREEIKNAL SNAWIDNAAL RTYRHSPNTG SRKSWAAGDA TSRGVHLALM SLKGEMGYPT 250 260 270 280 290 300 ALSAPGWGFQ DVLFNKKEIK LARPLDAYVM ENVLFKVSYP AEFHAQTAAE SAVILHPQVK 310 320 330 340 350 360 NRIDEIDRVV IRTHESAIRI IDKKGPLHNP ADRDHCLQYI TAIGLLFGDI TAQHYEAETA 370 380 390 400 410 420 NDPRIDKLRD KMEVTENKTY TEDYLKPDKR SISNAVQVHF KDGTSTEMVE CEFPLGHRFR 430 440 450 460 470 REEAVPKLLE KFSDNLKTHF PDKQHKHIYE RCTSYETLQT MRVNEFVDMF CM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the Bacillus subtilis genome containing the skin element and many sporulation genes." Mizuno M., Masuda S., Takemaru K., Hosono S., Sato T., Takeuchi M., Kobayashi Y. Microbiology 142:3103-3111(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168 / JH642.
[2]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[3]	"From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later." Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A. Microbiology 155:1758-1775(2009) [PubMed] [Europe PMC] [Abstract] Cited for: SEQUENCE REVISION TO 216.
[4]	"A sigma E dependent operon subject to catabolite repression during sporulation in Bacillus subtilis." Bryan E.M., Beall B.W., Moran C.P. Jr. J. Bacteriol. 178:4778-4786(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-178, DEVELOPMENTAL STAGE, INDUCTION. Strain: 168 / MB24.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D84432 Genomic DNA. Translation: BAA12591.1. AL009126 Genomic DNA. Translation: CAB14344.2. U29084 Genomic DNA. Translation: AAB09617.1.
PIR	F69658.
RefSeq	NP_390293.2. NC_000964.3.
3D structure databases
ProteinModelPortal	P45859.
SMR	P45859. Positions 5-472.
ModBase	Search...
Protein-protein interaction databases
STRING	224308.BSU24130.
Proteomic databases
PaxDb	P45859.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAB14344; CAB14344; BSU24130.
GeneID	938663.
KEGG	bsu:BSU24130.
PATRIC	18976654. VBIBacSub10457_2517.
Organism-specific databases
GenoList	BSU24130. [Micado]
Phylogenomic databases
eggNOG	COG2079.
HOGENOM	HOG000159916.
KO	K01720.
ProtClustDB	PRK09425.
Enzyme and pathway databases
BioCyc	BSUB:BSU24130-MONOMER.
UniPathway	UPA00946.
Family and domain databases
InterPro	IPR012705. 2Me_IsoCit_deHydtase_PrpD. IPR005656. MmgE_PrpD. [Graphical view]
PANTHER	PTHR16943. PTHR16943. 1 hit.
Pfam	PF03972. MmgE_PrpD. 1 hit. [Graphical view]
SUPFAM	SSF103378. MmgE_PrpD. 1 hit.
TIGRFAMs	TIGR02330. prpD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PRPD_BACSU

Accession

Primary (citable) accession number: P45859

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	July 7, 2009
Last modified:	May 1, 2013
This is version 89 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program