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P45859 (PRPD_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
2-methylcitrate dehydratase

EC=4.2.1.79
Gene names
Name:prpD
Synonyms:mmgE, yqiP
Ordered Locus Names:BSU24130
OrganismBacillus subtilis (strain 168) [Reference proteome] [HAMAP]
Taxonomic identifier224308 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length472 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the dehydration of 2-methylcitrate to 2-methyl-cis-aconitate By similarity.

Catalytic activity

(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate = (Z)-but-2-ene-1,2,3-tricarboxylate + H2O.

Cofactor

Binds 1 2Fe-2S cluster By similarity.

Pathway

Organic acid metabolism; propanoate degradation.

Developmental stage

Expressed in the mother cell at intermediate stages of sporulation under the control of the sigma-E factor. Ref.4

Induction

Subject to catabolite repression. Ref.4

Sequence similarities

Belongs to the PrpD family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 4724722-methylcitrate dehydratase
PRO_0000215020

Experimental info

Sequence conflict2161A → P in BAA12591. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P45859 [UniParc].

Last modified July 7, 2009. Version 3.
Checksum: 5941EA6EC1DA2142

FASTA47252,878
        10         20         30         40         50         60 
MPKTDRVIEE ITDYVLEKEI TSAEAYTTAG HVLLDTLGCG ILALRYPECT KLLGPIVPGT 

        70         80         90        100        110        120 
TVPNGSKVPG TSYVLDPVRA AFNIGCMIRW LDYNDTWLAA EWGHPSDNLG GILAAADYVS 

       130        140        150        160        170        180 
RVRLSEGKEP LTVRDVLEMM IKAHEIQGVL ALENSLNRVG LDHVLFVKVA TTAVAAKLLG 

       190        200        210        220        230        240 
GGREEIKNAL SNAWIDNAAL RTYRHSPNTG SRKSWAAGDA TSRGVHLALM SLKGEMGYPT 

       250        260        270        280        290        300 
ALSAPGWGFQ DVLFNKKEIK LARPLDAYVM ENVLFKVSYP AEFHAQTAAE SAVILHPQVK 

       310        320        330        340        350        360 
NRIDEIDRVV IRTHESAIRI IDKKGPLHNP ADRDHCLQYI TAIGLLFGDI TAQHYEAETA 

       370        380        390        400        410        420 
NDPRIDKLRD KMEVTENKTY TEDYLKPDKR SISNAVQVHF KDGTSTEMVE CEFPLGHRFR 

       430        440        450        460        470 
REEAVPKLLE KFSDNLKTHF PDKQHKHIYE RCTSYETLQT MRVNEFVDMF CM 

« Hide

References

« Hide 'large scale' references
[1]"Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the Bacillus subtilis genome containing the skin element and many sporulation genes."
Mizuno M., Masuda S., Takemaru K., Hosono S., Sato T., Takeuchi M., Kobayashi Y.
Microbiology 142:3103-3111(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168 / JH642.
[2]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[3]"From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later."
Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.
Microbiology 155:1758-1775(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: SEQUENCE REVISION TO 216.
[4]"A sigma E dependent operon subject to catabolite repression during sporulation in Bacillus subtilis."
Bryan E.M., Beall B.W., Moran C.P. Jr.
J. Bacteriol. 178:4778-4786(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-178, DEVELOPMENTAL STAGE, INDUCTION.
Strain: 168 / MB24.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D84432 Genomic DNA. Translation: BAA12591.1.
AL009126 Genomic DNA. Translation: CAB14344.2.
U29084 Genomic DNA. Translation: AAB09617.1.
PIRF69658.
RefSeqNP_390293.2. NC_000964.3.

3D structure databases

ProteinModelPortalP45859.
SMRP45859. Positions 5-472.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224308.BSU24130.

Proteomic databases

PaxDbP45859.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB14344; CAB14344; BSU24130.
GeneID938663.
KEGGbsu:BSU24130.
PATRIC18976654. VBIBacSub10457_2517.

Organism-specific databases

GenoListBSU24130. [Micado]

Phylogenomic databases

eggNOGCOG2079.
HOGENOMHOG000159916.
KOK01720.
OrthoDBEOG6RC3K5.
ProtClustDBPRK09425.

Enzyme and pathway databases

BioCycBSUB:BSU24130-MONOMER.
UniPathwayUPA00946.

Family and domain databases

InterProIPR012705. 2Me_IsoCit_deHydtase_PrpD.
IPR005656. MmgE_PrpD.
[Graphical view]
PANTHERPTHR16943. PTHR16943. 1 hit.
PfamPF03972. MmgE_PrpD. 1 hit.
[Graphical view]
SUPFAMSSF103378. SSF103378. 1 hit.
TIGRFAMsTIGR02330. prpD. 1 hit.
ProtoNetSearch...

Entry information

Entry namePRPD_BACSU
AccessionPrimary (citable) accession number: P45859
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: July 7, 2009
Last modified: November 13, 2013
This is version 91 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList