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P45859

- PRPD_BACSU

UniProt

P45859 - PRPD_BACSU

Protein

2-methylcitrate dehydratase

Gene

prpD

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 3 (07 Jul 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the dehydration of 2-methylcitrate to 2-methyl-cis-aconitate.By similarity

    Catalytic activityi

    (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate = (Z)-but-2-ene-1,2,3-tricarboxylate + H2O.

    Cofactori

    Binds 1 2Fe-2S cluster.By similarity

    Pathwayi

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. 2-methylcitrate dehydratase activity Source: UniProtKB-EC
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. propionate catabolic process, 2-methylcitrate cycle Source: InterPro
    2. sporulation resulting in formation of a cellular spore Source: UniProtKB-KW

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Sporulation

    Keywords - Ligandi

    2Fe-2S, Iron, Iron-sulfur, Metal-binding

    Enzyme and pathway databases

    BioCyciBSUB:BSU24130-MONOMER.
    UniPathwayiUPA00946.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    2-methylcitrate dehydratase (EC:4.2.1.79)
    Gene namesi
    Name:prpD
    Synonyms:mmgE, yqiP
    Ordered Locus Names:BSU24130
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000001570: Chromosome

    Organism-specific databases

    GenoListiBSU24130. [Micado]

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 4724722-methylcitrate dehydratasePRO_0000215020Add
    BLAST

    Proteomic databases

    PaxDbiP45859.

    Expressioni

    Developmental stagei

    Expressed in the mother cell at intermediate stages of sporulation under the control of the sigma-E factor.1 Publication

    Inductioni

    Subject to catabolite repression.1 Publication

    Interactioni

    Protein-protein interaction databases

    STRINGi224308.BSU24130.

    Structurei

    3D structure databases

    ProteinModelPortaliP45859.
    SMRiP45859. Positions 5-472.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PrpD family.Curated

    Phylogenomic databases

    eggNOGiCOG2079.
    HOGENOMiHOG000159916.
    KOiK01720.
    OrthoDBiEOG6RC3K5.
    PhylomeDBiP45859.

    Family and domain databases

    InterProiIPR012705. 2Me_IsoCit_deHydtase_PrpD.
    IPR005656. MmgE_PrpD.
    [Graphical view]
    PANTHERiPTHR16943. PTHR16943. 1 hit.
    PfamiPF03972. MmgE_PrpD. 1 hit.
    [Graphical view]
    SUPFAMiSSF103378. SSF103378. 1 hit.
    TIGRFAMsiTIGR02330. prpD. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P45859-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPKTDRVIEE ITDYVLEKEI TSAEAYTTAG HVLLDTLGCG ILALRYPECT    50
    KLLGPIVPGT TVPNGSKVPG TSYVLDPVRA AFNIGCMIRW LDYNDTWLAA 100
    EWGHPSDNLG GILAAADYVS RVRLSEGKEP LTVRDVLEMM IKAHEIQGVL 150
    ALENSLNRVG LDHVLFVKVA TTAVAAKLLG GGREEIKNAL SNAWIDNAAL 200
    RTYRHSPNTG SRKSWAAGDA TSRGVHLALM SLKGEMGYPT ALSAPGWGFQ 250
    DVLFNKKEIK LARPLDAYVM ENVLFKVSYP AEFHAQTAAE SAVILHPQVK 300
    NRIDEIDRVV IRTHESAIRI IDKKGPLHNP ADRDHCLQYI TAIGLLFGDI 350
    TAQHYEAETA NDPRIDKLRD KMEVTENKTY TEDYLKPDKR SISNAVQVHF 400
    KDGTSTEMVE CEFPLGHRFR REEAVPKLLE KFSDNLKTHF PDKQHKHIYE 450
    RCTSYETLQT MRVNEFVDMF CM 472
    Length:472
    Mass (Da):52,878
    Last modified:July 7, 2009 - v3
    Checksum:i5941EA6EC1DA2142
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti216 – 2161A → P in BAA12591. (PubMed:8969508)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D84432 Genomic DNA. Translation: BAA12591.1.
    AL009126 Genomic DNA. Translation: CAB14344.2.
    U29084 Genomic DNA. Translation: AAB09617.1.
    PIRiF69658.
    RefSeqiNP_390293.2. NC_000964.3.

    Genome annotation databases

    EnsemblBacteriaiCAB14344; CAB14344; BSU24130.
    GeneIDi938663.
    KEGGibsu:BSU24130.
    PATRICi18976654. VBIBacSub10457_2517.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D84432 Genomic DNA. Translation: BAA12591.1 .
    AL009126 Genomic DNA. Translation: CAB14344.2 .
    U29084 Genomic DNA. Translation: AAB09617.1 .
    PIRi F69658.
    RefSeqi NP_390293.2. NC_000964.3.

    3D structure databases

    ProteinModelPortali P45859.
    SMRi P45859. Positions 5-472.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224308.BSU24130.

    Proteomic databases

    PaxDbi P45859.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAB14344 ; CAB14344 ; BSU24130 .
    GeneIDi 938663.
    KEGGi bsu:BSU24130.
    PATRICi 18976654. VBIBacSub10457_2517.

    Organism-specific databases

    GenoListi BSU24130. [Micado ]

    Phylogenomic databases

    eggNOGi COG2079.
    HOGENOMi HOG000159916.
    KOi K01720.
    OrthoDBi EOG6RC3K5.
    PhylomeDBi P45859.

    Enzyme and pathway databases

    UniPathwayi UPA00946 .
    BioCyci BSUB:BSU24130-MONOMER.

    Family and domain databases

    InterProi IPR012705. 2Me_IsoCit_deHydtase_PrpD.
    IPR005656. MmgE_PrpD.
    [Graphical view ]
    PANTHERi PTHR16943. PTHR16943. 1 hit.
    Pfami PF03972. MmgE_PrpD. 1 hit.
    [Graphical view ]
    SUPFAMi SSF103378. SSF103378. 1 hit.
    TIGRFAMsi TIGR02330. prpD. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the Bacillus subtilis genome containing the skin element and many sporulation genes."
      Mizuno M., Masuda S., Takemaru K., Hosono S., Sato T., Takeuchi M., Kobayashi Y.
      Microbiology 142:3103-3111(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168 / JH642.
    2. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
      Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
      , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
      Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 168.
    3. "From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later."
      Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.
      Microbiology 155:1758-1775(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: SEQUENCE REVISION TO 216.
    4. "A sigma E dependent operon subject to catabolite repression during sporulation in Bacillus subtilis."
      Bryan E.M., Beall B.W., Moran C.P. Jr.
      J. Bacteriol. 178:4778-4786(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-178, DEVELOPMENTAL STAGE, INDUCTION.
      Strain: 168 / MB24.

    Entry informationi

    Entry nameiPRPD_BACSU
    AccessioniPrimary (citable) accession number: P45859
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: July 7, 2009
    Last modified: October 1, 2014
    This is version 94 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3