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P45859

- PRPD_BACSU

UniProt

P45859 - PRPD_BACSU

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Protein

2-methylcitrate dehydratase

Gene

prpD

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Catalyzes the dehydration of 2-methylcitrate to 2-methyl-cis-aconitate.By similarity

Catalytic activityi

(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate = (Z)-but-2-ene-1,2,3-tricarboxylate + H2O.

Cofactori

Binds 1 2Fe-2S cluster.By similarity

Pathwayi

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 2-methylcitrate dehydratase activity Source: UniProtKB-EC
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. propionate catabolic process, 2-methylcitrate cycle Source: InterPro
  2. sporulation resulting in formation of a cellular spore Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Sporulation

Keywords - Ligandi

2Fe-2S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciBSUB:BSU24130-MONOMER.
UniPathwayiUPA00946.

Names & Taxonomyi

Protein namesi
Recommended name:
2-methylcitrate dehydratase (EC:4.2.1.79)
Gene namesi
Name:prpD
Synonyms:mmgE, yqiP
Ordered Locus Names:BSU24130
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001570: Chromosome

Organism-specific databases

GenoListiBSU24130. [Micado]

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4724722-methylcitrate dehydratasePRO_0000215020Add
BLAST

Proteomic databases

PaxDbiP45859.

Expressioni

Developmental stagei

Expressed in the mother cell at intermediate stages of sporulation under the control of the sigma-E factor.1 Publication

Inductioni

Subject to catabolite repression.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi224308.BSU24130.

Structurei

3D structure databases

ProteinModelPortaliP45859.
SMRiP45859. Positions 5-472.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PrpD family.Curated

Phylogenomic databases

eggNOGiCOG2079.
HOGENOMiHOG000159916.
InParanoidiP45859.
KOiK01720.
OrthoDBiEOG6RC3K5.
PhylomeDBiP45859.

Family and domain databases

InterProiIPR012705. 2Me_IsoCit_deHydtase_PrpD.
IPR005656. MmgE_PrpD.
[Graphical view]
PANTHERiPTHR16943. PTHR16943. 1 hit.
PfamiPF03972. MmgE_PrpD. 1 hit.
[Graphical view]
SUPFAMiSSF103378. SSF103378. 1 hit.
TIGRFAMsiTIGR02330. prpD. 1 hit.

Sequencei

Sequence statusi: Complete.

P45859-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPKTDRVIEE ITDYVLEKEI TSAEAYTTAG HVLLDTLGCG ILALRYPECT
60 70 80 90 100
KLLGPIVPGT TVPNGSKVPG TSYVLDPVRA AFNIGCMIRW LDYNDTWLAA
110 120 130 140 150
EWGHPSDNLG GILAAADYVS RVRLSEGKEP LTVRDVLEMM IKAHEIQGVL
160 170 180 190 200
ALENSLNRVG LDHVLFVKVA TTAVAAKLLG GGREEIKNAL SNAWIDNAAL
210 220 230 240 250
RTYRHSPNTG SRKSWAAGDA TSRGVHLALM SLKGEMGYPT ALSAPGWGFQ
260 270 280 290 300
DVLFNKKEIK LARPLDAYVM ENVLFKVSYP AEFHAQTAAE SAVILHPQVK
310 320 330 340 350
NRIDEIDRVV IRTHESAIRI IDKKGPLHNP ADRDHCLQYI TAIGLLFGDI
360 370 380 390 400
TAQHYEAETA NDPRIDKLRD KMEVTENKTY TEDYLKPDKR SISNAVQVHF
410 420 430 440 450
KDGTSTEMVE CEFPLGHRFR REEAVPKLLE KFSDNLKTHF PDKQHKHIYE
460 470
RCTSYETLQT MRVNEFVDMF CM
Length:472
Mass (Da):52,878
Last modified:July 7, 2009 - v3
Checksum:i5941EA6EC1DA2142
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti216 – 2161A → P in BAA12591. (PubMed:8969508)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D84432 Genomic DNA. Translation: BAA12591.1.
AL009126 Genomic DNA. Translation: CAB14344.2.
U29084 Genomic DNA. Translation: AAB09617.1.
PIRiF69658.
RefSeqiNP_390293.2. NC_000964.3.

Genome annotation databases

EnsemblBacteriaiCAB14344; CAB14344; BSU24130.
GeneIDi938663.
KEGGibsu:BSU24130.
PATRICi18976654. VBIBacSub10457_2517.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D84432 Genomic DNA. Translation: BAA12591.1 .
AL009126 Genomic DNA. Translation: CAB14344.2 .
U29084 Genomic DNA. Translation: AAB09617.1 .
PIRi F69658.
RefSeqi NP_390293.2. NC_000964.3.

3D structure databases

ProteinModelPortali P45859.
SMRi P45859. Positions 5-472.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224308.BSU24130.

Proteomic databases

PaxDbi P45859.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAB14344 ; CAB14344 ; BSU24130 .
GeneIDi 938663.
KEGGi bsu:BSU24130.
PATRICi 18976654. VBIBacSub10457_2517.

Organism-specific databases

GenoListi BSU24130. [Micado ]

Phylogenomic databases

eggNOGi COG2079.
HOGENOMi HOG000159916.
InParanoidi P45859.
KOi K01720.
OrthoDBi EOG6RC3K5.
PhylomeDBi P45859.

Enzyme and pathway databases

UniPathwayi UPA00946 .
BioCyci BSUB:BSU24130-MONOMER.

Family and domain databases

InterProi IPR012705. 2Me_IsoCit_deHydtase_PrpD.
IPR005656. MmgE_PrpD.
[Graphical view ]
PANTHERi PTHR16943. PTHR16943. 1 hit.
Pfami PF03972. MmgE_PrpD. 1 hit.
[Graphical view ]
SUPFAMi SSF103378. SSF103378. 1 hit.
TIGRFAMsi TIGR02330. prpD. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the Bacillus subtilis genome containing the skin element and many sporulation genes."
    Mizuno M., Masuda S., Takemaru K., Hosono S., Sato T., Takeuchi M., Kobayashi Y.
    Microbiology 142:3103-3111(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168 / JH642.
  2. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  3. "From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later."
    Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.
    Microbiology 155:1758-1775(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: SEQUENCE REVISION TO 216.
  4. "A sigma E dependent operon subject to catabolite repression during sporulation in Bacillus subtilis."
    Bryan E.M., Beall B.W., Moran C.P. Jr.
    J. Bacteriol. 178:4778-4786(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-178, DEVELOPMENTAL STAGE, INDUCTION.
    Strain: 168 / MB24.

Entry informationi

Entry nameiPRPD_BACSU
AccessioniPrimary (citable) accession number: P45859
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: July 7, 2009
Last modified: October 29, 2014
This is version 95 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3