Reviewed, UniProtKB/Swiss-Prot P45851 (OXO2_HORVU)

Names and origin

Protein names	Recommended name:     Oxalate oxidase 2     EC=1.2.3.4 Alternative name(s):     Germin
Organism	Hordeum vulgare (Barley)
Taxonomic identifier	4513 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Pooideae › Triticeae › Hordeum

Protein attributes

Sequence length	224 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Releases hydrogen peroxide in the apoplast. May play an important role in several aspects of plant growth and defense mechanisms.
Catalytic activity	Oxalate + O2 + 2 H+ = 2 CO2 + H2O2.
Subunit structure	Oligomer (believed to be a pentamer but probably hexamer) By similarity.
Subcellular location	Secreted › extracellular space › apoplast By similarity. Secreted › cell wall By similarity.
Tissue specificity	Root.
Induction	By salt stress.
Post-translational modification	Glycosylated. A form called G contains antennary GlcNAc residues, whereas a form called G' lacks antennary GlcNAc residues in its otherwise identical glycans.
Sequence similarities	Belongs to the germin family.

Ontologies

Keywords
Biological process	Stress response
Cellular component	Apoplast Cell wall Secreted
Domain	Signal
Ligand	Manganese Metal-binding
Molecular function	Oxidoreductase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW response to stress Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	apoplast Inferred from electronic annotation. Source: UniProtKB-SubCell cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW nutrient reservoir activity Inferred from electronic annotation. Source: InterPro oxalate oxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	By similarity
	Chain	24 – 224	201	Oxalate oxidase 2		PRO_0000010833
Sites
	Metal binding	111	1	Manganese By similarity
	Metal binding	113	1	Manganese By similarity
	Metal binding	118	1	Manganese By similarity
	Metal binding	160	1	Manganese By similarity
Amino acid modifications
	Glycosylation	70	1	N-linked (GlcNAc...) Potential
	Glycosylation	75	1	N-linked (GlcNAc...) Potential
	Disulfide bond	33 ↔ 49		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P45851-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 425B69B31C1CAECF	FASTA	224	23,479
	10 20 30 40 50 60 MGYSKTLAVS LFAVLLLAPA VLASDPDPLQ DFCVADLDGK AVSVNGHPCK PMSEAGDDFL 70 80 90 100 110 120 FSSKLAKAGN TSTPNGSAVT ELDVAEWPGT NTLGVSMNRV DFAPGGTNPP HVHPRATEIG 130 140 150 160 170 180 IVMKGELLVG ILGSLDSGNK LYSRVVRAGE TFLIPRGLMH FQFNVGKTEA SMVVSFNSQN 190 200 210 220 PGIVFVPLTL FGSNPPIPTP VLTKALRVEA GVVELLKSKF AAGF

References

[1]

"Nucleotide sequence of a transcript encoding a germin-like protein that is present in salt-stressed barley (Hordeum vulgare L.) roots." Hurkman W.J., Lane B.G., Tanaka C.K. Plant Physiol. 104:803-804(1994) [PubMed: 8159797] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. CM 72. Tissue: Root.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U01963 mRNA. Translation: AAA20245.1.
3D structure databases
HSSP	HSSP built from PDB template 1FI2 based on UniProtKB P45850.
SMR	P45851. Positions 24-223.
ModBase	Search...
Organism-specific databases
Gramene	P45851.
Enzyme and pathway databases
BRENDA	1.2.3.4. 283.
Gene expression databases
Genevestigator	P45851.
Family and domain databases
InterPro	IPR006045. Cupin_1. IPR001929. Germin. IPR019780. Germin_Mn-BS. IPR014710. RmlC-like_jellyroll. [Graphical view]
Gene3D	G3DSA:2.60.120.10. RmlC-like_jellyroll. 1 hit.
Pfam	PF00190. Cupin_1. 1 hit. [Graphical view]
PRINTS	PR00325. GERMIN.
PROSITE	PS00725. GERMIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

OXO2_HORVU

Accession

Primary (citable) accession number: P45851

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	October 13, 2009
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)