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Protein

Oxalate oxidase 1

Gene
N/A
Organism
Hordeum vulgare (Barley)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Releases hydrogen peroxide in the apoplast which may be important for cross-linking reactions in the cell wall biochemistry. May play an important role in several aspects of plant growth and defense mechanisms.

Catalytic activityi

Oxalate + O2 + 2 H+ = 2 CO2 + H2O2.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi88 – 881Manganese
Metal bindingi90 – 901Manganese
Metal bindingi95 – 951Manganese
Metal bindingi137 – 1371Manganese

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Cell wall biogenesis/degradation, Stress response

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16186.
BRENDAi1.2.3.4. 2687.
SABIO-RKP45850.

Protein family/group databases

MoonProtiP45850.

Names & Taxonomyi

Protein namesi
Recommended name:
Oxalate oxidase 1 (EC:1.2.3.4)
Alternative name(s):
Germin
OrganismiHordeum vulgare (Barley)
Taxonomic identifieri4513 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladePooideaeTriticodaeTriticeaeHordeinaeHordeum

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Apoplast, Cell wall, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 201201Oxalate oxidase 1PRO_0000192013Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi10 ↔ 26
Glycosylationi47 – 471N-linked (GlcNAc...)
Glycosylationi52 – 521N-linked (GlcNAc...)Sequence analysis

Post-translational modificationi

Glycosylated. A form called G contains antennary GlcNAc residues, whereas a form called G' lacks antennary GlcNAc residues in its otherwise identical glycans.

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Inductioni

By salt stress.

Interactioni

Subunit structurei

Hexamer.

Protein-protein interaction databases

STRINGi4513.MLOC_75098.2.

Structurei

Secondary structure

1
201
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi6 – 83Combined sources
Helixi29 – 313Combined sources
Beta strandi53 – 608Combined sources
Turni61 – 633Combined sources
Helixi65 – 673Combined sources
Beta strandi73 – 797Combined sources
Beta strandi84 – 896Combined sources
Beta strandi95 – 10915Combined sources
Helixi112 – 1143Combined sources
Beta strandi118 – 1247Combined sources
Beta strandi128 – 1314Combined sources
Beta strandi137 – 1415Combined sources
Beta strandi143 – 1453Combined sources
Beta strandi147 – 15610Combined sources
Helixi163 – 1697Combined sources
Helixi176 – 1838Combined sources
Helixi187 – 19610Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1FI2X-ray1.60A1-201[»]
2ET1X-ray1.60A1-201[»]
2ET7X-ray1.70A1-201[»]
2ETEX-ray1.75A/B1-201[»]
ProteinModelPortaliP45850.
SMRiP45850. Positions 1-201.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP45850.

Family & Domainsi

Sequence similaritiesi

Belongs to the germin family.Curated

Phylogenomic databases

eggNOGiENOG410IW0A. Eukaryota.
ENOG410YKM8. LUCA.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
InterProiIPR006045. Cupin_1.
IPR001929. Germin.
IPR019780. Germin_Mn-BS.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PfamiPF00190. Cupin_1. 1 hit.
[Graphical view]
PRINTSiPR00325. GERMIN.
SMARTiSM00835. Cupin_1. 1 hit.
[Graphical view]
SUPFAMiSSF51182. SSF51182. 1 hit.
PROSITEiPS00725. GERMIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P45850-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
SDPDPLQDFC VADLDGKAVS VNGHTCKPMS EAGDDFLFSS KLTKAGNTST
60 70 80 90 100
PNGSAVTELD VAEWPGTNTL GVSMNRVDFA PGGTNPPHIH PRATEIGMVM
110 120 130 140 150
KGELLVGILG SLDSGNKLYS RVVRAGETFV IPRGLMHFQF NVGKTEAYMV
160 170 180 190 200
VSFNSQNPGI VFVPLTLFGS DPPIPTPVLT KALRVEAGVV ELLKSKFAGG

S
Length:201
Mass (Da):21,203
Last modified:November 1, 1995 - v1
Checksum:i76764D474CCB0F26
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L15737 mRNA. Translation: AAA32959.1.
PIRiA45980.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L15737 mRNA. Translation: AAA32959.1.
PIRiA45980.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1FI2X-ray1.60A1-201[»]
2ET1X-ray1.60A1-201[»]
2ET7X-ray1.70A1-201[»]
2ETEX-ray1.75A/B1-201[»]
ProteinModelPortaliP45850.
SMRiP45850. Positions 1-201.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi4513.MLOC_75098.2.

Protein family/group databases

MoonProtiP45850.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG410IW0A. Eukaryota.
ENOG410YKM8. LUCA.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16186.
BRENDAi1.2.3.4. 2687.
SABIO-RKP45850.

Miscellaneous databases

EvolutionaryTraceiP45850.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
InterProiIPR006045. Cupin_1.
IPR001929. Germin.
IPR019780. Germin_Mn-BS.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PfamiPF00190. Cupin_1. 1 hit.
[Graphical view]
PRINTSiPR00325. GERMIN.
SMARTiSM00835. Cupin_1. 1 hit.
[Graphical view]
SUPFAMiSSF51182. SSF51182. 1 hit.
PROSITEiPS00725. GERMIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Germin, a protein marker of early plant development, is an oxalate oxidase."
    Lane B.G., Dunwell J.M., Ray J.A., Schmitt M.R., Cuming A.C.
    J. Biol. Chem. 268:12239-12242(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 1-18.
    Tissue: Seedling root.
  2. "Germin is a manganese containing homohexamer with oxalate oxidase and superoxide dismutase activities."
    Woo E.-J., Dunwell J.M., Goodenough P.W., Marvier A.C., Pickersgill R.W.
    Nat. Struct. Biol. 7:1036-1040(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS).

Entry informationi

Entry nameiOXO1_HORVU
AccessioniPrimary (citable) accession number: P45850
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: February 17, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.