Reviewed, UniProtKB/Swiss-Prot P45850 (OXO1_HORVU)

Names and origin

Protein names	Recommended name:     Oxalate oxidase 1     EC=1.2.3.4 Alternative name(s):     Germin
Organism	Hordeum vulgare (Barley)
Taxonomic identifier	4513 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Pooideae › Triticeae › Hordeum

Protein attributes

Sequence length	201 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Releases hydrogen peroxide in the apoplast which may be important for cross-linking reactions in the cell wall biochemistry. May play an important role in several aspects of plant growth and defense mechanisms.
Catalytic activity	Oxalate + O2 + 2 H+ = 2 CO2 + H2O2.
Subunit structure	Hexamer.
Subcellular location	Secreted › extracellular space › apoplast By similarity. Secreted › cell wall By similarity.
Induction	By salt stress.
Post-translational modification	Glycosylated. A form called G contains antennary GlcNAc residues, whereas a form called G' lacks antennary GlcNAc residues in its otherwise identical glycans.
Sequence similarities	Belongs to the germin family.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation Stress response
Cellular component	Apoplast Cell wall Secreted
Ligand	Manganese Metal-binding
Molecular function	Oxidoreductase
PTM	Disulfide bond Glycoprotein
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW response to stress Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	apoplast Inferred from electronic annotation. Source: UniProtKB-SubCell cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW nutrient reservoir activity Inferred from electronic annotation. Source: InterPro oxalate oxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 201

201

Oxalate oxidase 1

PRO_0000192013

Sites

Metal binding

88

1

Manganese

Metal binding

90

1

Manganese

Metal binding

95

1

Manganese

Metal binding

137

1

Manganese

Amino acid modifications

Glycosylation

47

1

N-linked (GlcNAc...)

Glycosylation

52

1

N-linked (GlcNAc...) Potential

Disulfide bond

10 ↔ 26

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

201

Helix Strand Turn

Details...

Beta strand

6 – 8

3

Helix

29 – 31

3

Beta strand

53 – 60

8

Turn

61 – 63

3

Helix

65 – 67

3

Beta strand

73 – 79

7

Beta strand

84 – 89

6

Beta strand

95 – 109

15

Helix

112 – 114

3

Beta strand

117 – 124

8

Beta strand

128 – 131

4

Beta strand

137 – 141

5

Beta strand

143 – 145

3

Beta strand

147 – 156

10

Helix

163 – 169

7

Helix

176 – 183

8

Helix

187 – 196

10

Sequences

	Sequence		Length	Mass (Da)
	P45850-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 76764D474CCB0F26	FASTA	201	21,203
	10 20 30 40 50 60 SDPDPLQDFC VADLDGKAVS VNGHTCKPMS EAGDDFLFSS KLTKAGNTST PNGSAVTELD 70 80 90 100 110 120 VAEWPGTNTL GVSMNRVDFA PGGTNPPHIH PRATEIGMVM KGELLVGILG SLDSGNKLYS 130 140 150 160 170 180 RVVRAGETFV IPRGLMHFQF NVGKTEAYMV VSFNSQNPGI VFVPLTLFGS DPPIPTPVLT 190 200 KALRVEAGVV ELLKSKFAGG S

References

[1]	"Germin, a protein marker of early plant development, is an oxalate oxidase." Lane B.G., Dunwell J.M., Ray J.A., Schmitt M.R., Cuming A.C. J. Biol. Chem. 268:12239-12242(1993) [PubMed: 8509360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 1-18. Tissue: Seedling root.
[2]	"Germin is a manganese containing homohexamer with oxalate oxidase and superoxide dismutase activities." Woo E.-J., Dunwell J.M., Goodenough P.W., Marvier A.C., Pickersgill R.W. Nat. Struct. Biol. 7:1036-1040(2000) [PubMed: 11062559] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

L15737 mRNA. Translation: AAA32959.1.

PIR

A45980.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1FI2	X-ray	1.60	A	2-201	[»]
2ET1	X-ray	1.60	A	2-201	[»]
2ET7	X-ray	1.70	A	1-201	[»]
2ETE	X-ray	1.75	A/B	1-201	[»]

ModBase

Search...

Organism-specific databases

Gramene

P45850.

Enzyme and pathway databases

BRENDA

1.2.3.4. 283.

Gene expression databases

Genevestigator

P45850.

Family and domain databases

InterPro

IPR006045. Cupin_1.
IPR011051. Cupin_RmlC_type.
IPR001929. Germin.
IPR019780. Germin_Mn-BS.
IPR014710. RmlC-like_jellyroll.
[Graphical view]

Gene3D

G3DSA:2.60.120.10. RmlC-like_jellyroll. 1 hit.

Pfam

PF00190. Cupin_1. 1 hit.
[Graphical view]

PRINTS

PR00325. GERMIN.

SMART

SM00835. Cupin_1. 1 hit.
[Graphical view]

PROSITE

PS00725. GERMIN. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

OXO1_HORVU

Accession

Primary (citable) accession number: P45850

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	November 24, 2009
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)