P45840 (SYR_MYCLE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Arginine--tRNA ligase
EC=6.1.1.19

Alternative name(s):
Arginyl-tRNA synthetase
Short name=ArgRS

Gene names

Name:	argS
Ordered Locus Names:	ML1127

Organism

Mycobacterium leprae [Complete proteome] [HAMAP]

Taxonomic identifier

1769 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	550 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg). HAMAP MF_00123
Subunit structure	Monomer By similarity. HAMAP MF_00123
Subcellular location	Cytoplasm HAMAP MF_00123.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.
Sequence caution	The sequence AAA63099.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW arginine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 550	550	Arginine--tRNA ligase HAMAP MF_00123		PRO_0000151577
Regions
	Motif	130 – 140	11	"HIGH" region HAMAP MF_00123
Experimental info
	Sequence conflict	174	1	I → V in AAA63099. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P45840 [UniParc]. Last modified April 27, 2001. Version 3. Checksum: DBD8A95A9B9831BB	FASTA	550	59,603
	10 20 30 40 50 60 MTPADLAELL KTTAIVVLAE RGLDAAALPQ TVTVERPRNP EHGDYSSNLA MQLGKKVGAN 70 80 90 100 110 120 PLELAGWLAE VLAQAGGIAD VEVAGPGFIN MRLDASAQAM IVNTVINADK NFGHSDDLAG 130 140 150 160 170 180 YQINLEFVSA NPTGPIHIGG TRWAAVGDAL GRLLSTQGAA VVREYYFNDH GAQIDRFTNS 190 200 210 220 230 240 LIAAAKGELT PADGYAGTYV TDIAAQVMQQ APYALSLPES EMHETVREIG VDLMFTHIKK 250 260 270 280 290 300 SLHEFGTDFD VYTHEDSMHA SGRVDEAIAR LRDTGNVYEK DGALWLRTSA FGDDKDRVVI 310 320 330 340 350 360 KSDGKPAYIA GDLAYYLDKR QRGFDLCIYM LGADHHGYIA RLKAAAAAFG DDPAIVEVLI 370 380 390 400 410 420 GQMVNLVCDG QLVRMSKRSG NVITLDDLVE AIGVDAARYS LIRSSVDTPI DIDLALWSSS 430 440 450 460 470 480 SNENPVYYVQ YAHARLSALA RNAAEFGLIP DTGHLELLSH DKEGALLRTV GEFPQVLKTA 490 500 510 520 530 540 AALREPHRVC RYLEDLAGDY HRFYDSCRVL PQGDEKPTDL HTARLALCQA NRQVIANGLA 550 ILGVSAPERM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Smith D.R., Robison K. Submitted (SEP-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Massive gene decay in the leprosy bacillus." Cole S.T., Eiglmeier K., Parkhill J., James K.D., Thomson N.R., Wheeler P.R., Honore N., Garnier T., Churcher C.M., Harris D.E., Mungall K.L., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K., Duthoy S. , Feltwell T., Fraser A., Hamlin N., Holroyd S., Hornsby T., Jagels K., Lacroix C., Maclean J., Moule S., Murphy L.D., Oliver K., Quail M.A., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Simon S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Taylor K., Whitehead S., Woodward J.R., Barrell B.G. Nature 409:1007-1011(2001) [PubMed: 11234002] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TN.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U15186 Genomic DNA. Translation: AAA63099.1. Different initiation. AL583920 Genomic DNA. Translation: CAC31508.1.
PIR	A87050.
RefSeq	NP_301821.1. NC_002677.1.
3D structure databases
ProteinModelPortal	P45840.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000029117; EBMYCP00000028723; EBMYCG00000029112.
GeneID	910221.
GenomeReviews	Gene locus ML1127 in contig AL450380_GR.
KEGG	mle:ML1127.
NMPDR	fig|272631.1.peg.693.
PATRIC	18054209. VBIMycLep78757_2049.
Organism-specific databases
Leproma	ML1127.
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000016532.
HOGENOM	HBG695395.
OMA	FDVYFHE.
ProtClustDB	PRK01611.
Enzyme and pathway databases
BioCyc	MLEP272631:ML1127-MONOMER.
Family and domain databases
HAMAP	MF_00123. Arg_tRNA_synth. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR001278. Arg-tRNA-synth_Ia. IPR015945. Arg-tRNA-synth_Ia_core. IPR005148. Arg-tRNA-synth_N. IPR008909. DALR_anticod-bd. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.30.1360.70. Arg-tRNA-synth_Ic_N. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01887.
PANTHER	PTHR11956. Arg_tRNA-synt_1c. 1 hit.
Pfam	PF03485. Arg_tRNA_synt_N. 1 hit. PF05746. DALR_1. 1 hit. PF00750. tRNA-synt_1d. 1 hit. [Graphical view]
PRINTS	PR01038. TRNASYNTHARG.
SMART	SM01016. Arg_tRNA_synt_N. 1 hit. SM00836. DALR_1. 1 hit. [Graphical view]
SUPFAM	SSF55190. Arg-tRNA-synth_Ic_N. 1 hit. SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00456. ArgS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYR_MYCLE

Accession

Primary (citable) accession number: P45840

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	April 27, 2001
Last modified:	January 25, 2012
This is version 86 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program