P45821 (MURA_MYCLE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-N-acetylglucosamine 1-carboxyvinyltransferase
EC=2.5.1.7

Alternative name(s):
Enoylpyruvate transferase
UDP-N-acetylglucosamine enolpyruvyl transferase
Short name=EPT

Gene names

Name:	murA
Synonyms:	murZ
Ordered Locus Names:	ML1150

Organism

Mycobacterium leprae (strain TN) [Complete proteome] [HAMAP]

Taxonomic identifier

272631 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium leprae

Protein attributes

Sequence length	418 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation. Adds enolpyruvyl to UDP-N-acetylglucosamine By similarity. HAMAP-Rule MF_00111
Catalytic activity	Phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine. HAMAP-Rule MF_00111
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00111
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the EPSP synthase family. MurA subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	UDP-N-acetylgalactosamine biosynthetic process Inferred from electronic annotation. Source: InterPro cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	UDP-N-acetylglucosamine 1-carboxyvinyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 418	418	UDP-N-acetylglucosamine 1-carboxyvinyltransferase HAMAP-Rule MF_00111		PRO_0000178889
Sites
	Active site	117	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	P45821 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 488A273A12DC74F5	FASTA	418	44,227
	10 20 30 40 50 60 MAERFVVTGG NRLSGEVTVG GAKNSVLKLM AATLLAEGTS TITNCPDILD VPLMAEVLRG 70 80 90 100 110 120 LGATVELYGD VARITSPDEP KYDADFAAVR QFRASVCVLG PLVGRCKQAR VALPGGDAIG 130 140 150 160 170 180 SRPLDMHQAG LRQLGARCNI EHGCVVASAE TLRGAEIQLE FPSVGATENI LMAAVVAEGV 190 200 210 220 230 240 TTIHNAAREP DVVDLCTMLN QMGAQVEGVG SPTMTITGVP RLYPTEHRVI GDRIVAATWG 250 260 270 280 290 300 IAAAMTRGDI AVTGVDPAHL QLVLHKLHDA GATVTQTDDS FRVAQYERPK AVNVATLPFP 310 320 330 340 350 360 GFPTDLQPMA IALTSIADGT SMITENVFEA RFRFVEEMIR LGADARTDGH HAVVRGLPQL 370 380 390 400 410 SSAPVWCSDI RAGAGLVLAG LVADGDTEVY DVFHIDRGYP LFVENLANLG AEIERVCL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Smith D.R., Robison K. Submitted (SEP-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Massive gene decay in the leprosy bacillus." Cole S.T., Eiglmeier K., Parkhill J., James K.D., Thomson N.R., Wheeler P.R., Honore N., Garnier T., Churcher C.M., Harris D.E., Mungall K.L., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K., Duthoy S. , Feltwell T., Fraser A., Hamlin N., Holroyd S., Hornsby T., Jagels K., Lacroix C., Maclean J., Moule S., Murphy L.D., Oliver K., Quail M.A., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Simon S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Taylor K., Whitehead S., Woodward J.R., Barrell B.G. Nature 409:1007-1011(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TN.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U15186 Genomic DNA. Translation: AAA63098.1. AL583920 Genomic DNA. Translation: CAC31531.1.
PIR	T09969.
RefSeq	NP_301844.1. NC_002677.1.
3D structure databases
ProteinModelPortal	P45821.
ModBase	Search...
Protein-protein interaction databases
STRING	272631.ML1150.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAC31531; CAC31531; CAC31531.
GeneID	910244.
KEGG	mle:ML1150.
PATRIC	18054257. VBIMycLep78757_2073.
Organism-specific databases
Leproma	ML1150.
CMR	Search...
Phylogenomic databases
eggNOG	COG0766.
HOGENOM	HOG000075602.
KO	K00790.
OMA	SIWALAP.
ProtClustDB	PRK09369.
Enzyme and pathway databases
UniPathway	UPA00219.
Family and domain databases
Gene3D	3.65.10.10. 2 hits.
HAMAP	MF_00111. MurA.
InterPro	IPR001986. Enolpyruvate_Tfrase_dom. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. IPR005750. UDP_GlcNAc_COvinyl_MurA. [Graphical view]
PANTHER	PTHR21090:SF4. PTHR21090:SF4. 1 hit.
Pfam	PF00275. EPSP_synthase. 1 hit. [Graphical view]
SUPFAM	SSF55205. RNA3'_cycl/enolpyr_transf_A/B. 1 hit.
TIGRFAMs	TIGR01072. murA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURA_MYCLE

Accession

Primary (citable) accession number: P45821

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program