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Protein

ATP-dependent RNA helicase ROK1

Gene

ROK1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

ATP-dependent RNA helicase involved in 40S ribosomal subunit biogenesis. Required for the processing and cleavage of 35S pre-rRNA at sites A0, A1, and A2, leading to mature 18S rRNA. May also have a gene-specific regulatory function since it affects nuclear fusion by regulating KAR4 expression and contributes with KEM1 to ISP-1 sensitivity.5 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi166 – 173ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent RNA helicase activity Source: SGD
  • RNA binding Source: UniProtKB-KW
  • RNA-dependent ATPase activity Source: SGD

GO - Biological processi

  • endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  • endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  • endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  • maturation of SSU-rRNA Source: SGD
  • RNA secondary structure unwinding Source: GO_Central
  • rRNA methylation Source: Reactome
  • snoRNA localization Source: SGD

Keywordsi

Molecular functionHelicase, Hydrolase, RNA-binding
Biological processRibosome biogenesis, rRNA processing
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30659-MONOMER
ReactomeiR-SCE-6791226 Major pathway of rRNA processing in the nucleolus and cytosol

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent RNA helicase ROK1 (EC:3.6.4.13)
Alternative name(s):
Rescuer of KEM1 protein 1
Gene namesi
Name:ROK1
Ordered Locus Names:YGL171W
ORF Names:G1651
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL171W
SGDiS000003139 ROK1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi166G → D: No cell growth and 2-fold decrease in ATPase activity in vitro. 1 Publication1
Mutagenesisi172K → G, R, S or W: Slow cell growth. 1 Publication1
Mutagenesisi172K → L: No cell growth and drastic decrease in ATPase activity in vitro. 1 Publication1
Mutagenesisi280D → A or V: Slow cell growth. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000550631 – 564ATP-dependent RNA helicase ROK1Add BLAST564

Proteomic databases

MaxQBiP45818
PaxDbiP45818
PRIDEiP45818
TopDownProteomicsiP45818

Interactioni

Subunit structurei

Interacts with the U3 snoRNA and is associated with the 90S and 40S pre-ribosomes. This association requires the presence of RRP5. Interacts also with OSH3.2 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi33082, 39 interactors
DIPiDIP-5111N
IntActiP45818, 21 interactors
MINTiP45818
STRINGi4932.YGL171W

Structurei

3D structure databases

ProteinModelPortaliP45818
SMRiP45818
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini153 – 333Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST181
Domaini344 – 506Helicase C-terminalPROSITE-ProRule annotationAdd BLAST163

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi122 – 150Q motifAdd BLAST29
Motifi280 – 283DEAD box4

Domaini

The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00550000074863
HOGENOMiHOG000242486
InParanoidiP45818
KOiK14779
OMAiQKAVFSA
OrthoDBiEOG092C1UEZ

Family and domain databases

CDDicd00079 HELICc, 1 hit
InterProiView protein in InterPro
IPR011545 DEAD/DEAH_box_helicase_dom
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
IPR000629 RNA-helicase_DEAD-box_CS
IPR014014 RNA_helicase_DEAD_Q_motif
PfamiView protein in Pfam
PF00270 DEAD, 1 hit
PF00271 Helicase_C, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF52540 SSF52540, 2 hits
PROSITEiView protein in PROSITE
PS00039 DEAD_ATP_HELICASE, 1 hit
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit
PS51195 Q_MOTIF, 1 hit

Sequencei

Sequence statusi: Complete.

P45818-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDIFRVLTRG ASVKKESGPK AKAADYSVIN GNDENHKEDN NESQIVKELD
60 70 80 90 100
FFRNKRIISK VEDDREKTTE NDSPNKEEKS GNDDGLIKPV ITNTVEASAL
110 120 130 140 150
RKSYKGNVSG IDIPLPIGSF EDLISRFSFD KRLLNNLIEN GFTEPTPIQC
160 170 180 190 200
ECIPVALNNR DVLACGPTGS GKTLAFLIPL VQQIIDDKQT AGLKGLIISP
210 220 230 240 250
TKELANQIFI ECFKLSYKIF LEKKRPLQVA LLSKSLGAKL KNKVVSDKKY
260 270 280 290 300
DIIISTPLRL IDVVKNEALD LSKVKHLIFD EADKLFDKTF VEQSDDILSA
310 320 330 340 350
CREPSLRKAM FSATIPSNVE EIAQSIMMDP VRVIIGHKEA ANTNIEQKLI
360 370 380 390 400
FCGNEEGKLI AIRQLVQEGE FKPPIIIFLE SITRAKALYH ELMYDRINVD
410 420 430 440 450
VIHAERTALQ RDRIIERFKT GELWCLICTD VLARGIDFKG VNLVINYDVP
460 470 480 490 500
GSSQAYVHRI GRTGRGGRSG KAITFYTKQD SVAIKPIINV MKQSGCEVSE
510 520 530 540 550
WMDKMAKMTR KEKESIKNGK AHKERKQITT VPKMDKAKRR RQQEMIAASK
560
RRKNEELSKK HFSK
Length:564
Mass (Da):63,653
Last modified:November 1, 1995 - v1
Checksum:iCACB950B8A15D5A2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z34901 Genomic DNA Translation: CAA84384.1
X85757 Genomic DNA Translation: CAA59758.1
Z72693 Genomic DNA Translation: CAA96883.1
BK006941 Genomic DNA Translation: DAA07942.1
PIRiS59649
RefSeqiNP_011344.1, NM_001181036.1

Genome annotation databases

EnsemblFungiiYGL171W; YGL171W; YGL171W
GeneIDi852704
KEGGisce:YGL171W

Similar proteinsi

Entry informationi

Entry nameiROK1_YEAST
AccessioniPrimary (citable) accession number: P45818
Secondary accession number(s): D6VTY1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: May 23, 2018
This is version 165 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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