Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P45818 (ROK1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 130. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ATP-dependent RNA helicase ROK1

EC=3.6.4.13
Alternative name(s):
Rescuer of KEM1 protein 1
Gene names
Name:ROK1
Ordered Locus Names:YGL171W
ORF Names:G1651
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length564 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

ATP-dependent RNA helicase involved in 40S ribosomal subunit biogenesis. Required for the processing and cleavage of 35S pre-rRNA at sites A0, A1, and A2, leading to mature 18S rRNA. May also have a gene-specific regulatory function since it affects nuclear fusion by regulating KAR4 expression and contributes with KEM1 to ISP-1 sensitivity. Ref.5 Ref.6 Ref.7 Ref.10 Ref.11

Catalytic activity

ATP + H2O = ADP + phosphate.

Subunit structure

Interacts with the U3 snoRNA and is associated with the 90S and 40S pre-ribosomes. This association requires the presence of RRP5. Interacts also with OSH3. Ref.8 Ref.12

Subcellular location

Nucleusnucleolus Ref.5.

Domain

The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.

Sequence similarities

Belongs to the DEAD box helicase family. DDX52/ROK1 subfamily.

Contains 1 helicase ATP-binding domain.

Contains 1 helicase C-terminal domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

OSH3P387133EBI-15686,EBI-12630

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 564564ATP-dependent RNA helicase ROK1
PRO_0000055063

Regions

Domain153 – 333181Helicase ATP-binding
Domain344 – 506163Helicase C-terminal
Nucleotide binding166 – 1738ATP By similarity
Motif122 – 15029Q motif
Motif280 – 2834DEAD box

Experimental info

Mutagenesis1661G → D: No cell growth and 2-fold decrease in ATPase activity in vitro. Ref.7
Mutagenesis1721K → G, R, S or W: Slow cell growth. Ref.7
Mutagenesis1721K → L: No cell growth and drastic decrease in ATPase activity in vitro. Ref.7
Mutagenesis2801D → A or V: Slow cell growth. Ref.7

Sequences

Sequence LengthMass (Da)Tools
P45818 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: CACB950B8A15D5A2

FASTA56463,653
        10         20         30         40         50         60 
MDIFRVLTRG ASVKKESGPK AKAADYSVIN GNDENHKEDN NESQIVKELD FFRNKRIISK 

        70         80         90        100        110        120 
VEDDREKTTE NDSPNKEEKS GNDDGLIKPV ITNTVEASAL RKSYKGNVSG IDIPLPIGSF 

       130        140        150        160        170        180 
EDLISRFSFD KRLLNNLIEN GFTEPTPIQC ECIPVALNNR DVLACGPTGS GKTLAFLIPL 

       190        200        210        220        230        240 
VQQIIDDKQT AGLKGLIISP TKELANQIFI ECFKLSYKIF LEKKRPLQVA LLSKSLGAKL 

       250        260        270        280        290        300 
KNKVVSDKKY DIIISTPLRL IDVVKNEALD LSKVKHLIFD EADKLFDKTF VEQSDDILSA 

       310        320        330        340        350        360 
CREPSLRKAM FSATIPSNVE EIAQSIMMDP VRVIIGHKEA ANTNIEQKLI FCGNEEGKLI 

       370        380        390        400        410        420 
AIRQLVQEGE FKPPIIIFLE SITRAKALYH ELMYDRINVD VIHAERTALQ RDRIIERFKT 

       430        440        450        460        470        480 
GELWCLICTD VLARGIDFKG VNLVINYDVP GSSQAYVHRI GRTGRGGRSG KAITFYTKQD 

       490        500        510        520        530        540 
SVAIKPIINV MKQSGCEVSE WMDKMAKMTR KEKESIKNGK AHKERKQITT VPKMDKAKRR 

       550        560 
RQQEMIAASK RRKNEELSKK HFSK 

« Hide

References

« Hide 'large scale' references
[1]"ROK1, a high-copy-number plasmid suppressor of kem1, encodes a putative ATP-dependent RNA helicase in Saccharomyces cerevisiae."
Song Y., Kim S., Kim J.
Gene 166:151-154(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"A putative helicase, the SUA5, PMR1, tRNALys1 genes and four open reading frames have been detected in the DNA sequence of an 8.8 kb fragment of the left arm of chromosome VII of Saccharomyces cerevisiae."
Klima R., Coglievina M., Zaccaria P., Bertani I., Bruschi C.V.
Yeast 12:1033-1040(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Rok1p is a putative RNA helicase required for rRNA processing."
Venema J., Bousquet-Antonelli C., Gelugne J.-P., Caizergues-Ferrer M., Tollervey D.
Mol. Cell. Biol. 17:3398-3407(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[6]"Two mutant forms of the S1/TPR-containing protein Rrp5p affect the 18S rRNA synthesis in Saccharomyces cerevisiae."
Torchet C., Jacq C., Hermann-Le Denmat S.
RNA 4:1636-1652(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"ATP hydrolysis activity of the DEAD box protein Rok1p is required for in vivo ROK1 function."
Oh J.Y., Kim J.
Nucleic Acids Res. 27:2753-2759(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, MUTAGENESIS OF GLY-166; LYS-172 AND ASP-280.
[8]"Two-hybrid cloning and characterization of OSH3, a yeast oxysterol-binding protein homolog."
Park Y.-U., Hwang O., Kim J.
Biochem. Biophys. Res. Commun. 293:733-740(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH OSH3.
[9]"The path from nucleolar 90S to cytoplasmic 40S pre-ribosomes."
Schaefer T., Strauss D., Petfalski E., Tollervey D., Hurt E.
EMBO J. 22:1370-1380(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: ASSOCIATION WITH THE 90S AND 40S PRE-RIBOSOMES, IDENTIFICATION BY MASS SPECTROMETRY.
[10]"Deletion of OSH3 gene confers resistance against ISP-1 in Saccharomyces cerevisiae."
Yano T., Inukai M., Isono F.
Biochem. Biophys. Res. Commun. 315:228-234(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[11]"Posttranscriptional regulation of the karyogamy gene by Kem1p/Xrn1p exoribonuclease and Rok1p RNA helicase of Saccharomyces cerevisiae."
Kim J., Jeon S., Yang Y.-S., Kim J.
Biochem. Biophys. Res. Commun. 321:1032-1039(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[12]"U3 snoRNP and Rrp5p associate independently with Saccharomyces cerevisiae 35S pre-rRNA, but Rrp5p is essential for association of Rok1p."
Vos H.R., Bax R., Faber A.W., Vos J.C., Raue H.A.
Nucleic Acids Res. 32:5827-5833(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH THE U3 SNORNA, ASSOCIATION WITH THE PRE-RIBOSOME.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z34901 Genomic DNA. Translation: CAA84384.1.
X85757 Genomic DNA. Translation: CAA59758.1.
Z72693 Genomic DNA. Translation: CAA96883.1.
BK006941 Genomic DNA. Translation: DAA07942.1.
PIRS59649.
RefSeqNP_011344.1. NM_001181036.1.

3D structure databases

ProteinModelPortalP45818.
SMRP45818. Positions 90-511.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33082. 33 interactions.
DIPDIP-5111N.
IntActP45818. 16 interactions.
MINTMINT-483312.
STRING4932.YGL171W.

Proteomic databases

PaxDbP45818.
PeptideAtlasP45818.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYGL171W; YGL171W; YGL171W.
GeneID852704.
KEGGsce:YGL171W.

Organism-specific databases

CYGDYGL171w.
SGDS000003139. ROK1.

Phylogenomic databases

eggNOGCOG0513.
GeneTreeENSGT00550000074863.
HOGENOMHOG000242486.
KOK14779.
OMAVMKQSGC.
OrthoDBEOG74TX7R.

Enzyme and pathway databases

BioCycYEAST:G3O-30659-MONOMER.

Gene expression databases

GenevestigatorP45818.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
InterProIPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 2 hits.
PROSITEPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio972056.
PROP45818.

Entry information

Entry nameROK1_YEAST
AccessionPrimary (citable) accession number: P45818
Secondary accession number(s): D6VTY1
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: April 16, 2014
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families