Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P45818

- ROK1_YEAST

UniProt

P45818 - ROK1_YEAST

Protein

ATP-dependent RNA helicase ROK1

Gene

ROK1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 133 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    ATP-dependent RNA helicase involved in 40S ribosomal subunit biogenesis. Required for the processing and cleavage of 35S pre-rRNA at sites A0, A1, and A2, leading to mature 18S rRNA. May also have a gene-specific regulatory function since it affects nuclear fusion by regulating KAR4 expression and contributes with KEM1 to ISP-1 sensitivity.5 Publications

    Catalytic activityi

    ATP + H2O = ADP + phosphate.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi166 – 1738ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. ATP-dependent RNA helicase activity Source: SGD
    3. protein binding Source: IntAct
    4. RNA binding Source: UniProtKB-KW
    5. RNA-dependent ATPase activity Source: SGD

    GO - Biological processi

    1. ATP catabolic process Source: GOC
    2. endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
    3. endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
    4. endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
    5. snoRNA localization Source: SGD

    Keywords - Molecular functioni

    Helicase, Hydrolase

    Keywords - Biological processi

    Ribosome biogenesis, rRNA processing

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding, RNA-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30659-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-dependent RNA helicase ROK1 (EC:3.6.4.13)
    Alternative name(s):
    Rescuer of KEM1 protein 1
    Gene namesi
    Name:ROK1
    Ordered Locus Names:YGL171W
    ORF Names:G1651
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    CYGDiYGL171w.
    SGDiS000003139. ROK1.

    Subcellular locationi

    Nucleusnucleolus 1 Publication

    GO - Cellular componenti

    1. nucleolus Source: SGD

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi166 – 1661G → D: No cell growth and 2-fold decrease in ATPase activity in vitro. 1 Publication
    Mutagenesisi172 – 1721K → G, R, S or W: Slow cell growth. 1 Publication
    Mutagenesisi172 – 1721K → L: No cell growth and drastic decrease in ATPase activity in vitro. 1 Publication
    Mutagenesisi280 – 2801D → A or V: Slow cell growth. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 564564ATP-dependent RNA helicase ROK1PRO_0000055063Add
    BLAST

    Proteomic databases

    MaxQBiP45818.
    PaxDbiP45818.
    PeptideAtlasiP45818.

    Expressioni

    Gene expression databases

    GenevestigatoriP45818.

    Interactioni

    Subunit structurei

    Interacts with the U3 snoRNA and is associated with the 90S and 40S pre-ribosomes. This association requires the presence of RRP5. Interacts also with OSH3.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    OSH3P387133EBI-15686,EBI-12630

    Protein-protein interaction databases

    BioGridi33082. 33 interactions.
    DIPiDIP-5111N.
    IntActiP45818. 16 interactions.
    MINTiMINT-483312.
    STRINGi4932.YGL171W.

    Structurei

    3D structure databases

    ProteinModelPortaliP45818.
    SMRiP45818. Positions 90-511.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini153 – 333181Helicase ATP-bindingPROSITE-ProRule annotationAdd
    BLAST
    Domaini344 – 506163Helicase C-terminalPROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi122 – 15029Q motifAdd
    BLAST
    Motifi280 – 2834DEAD box

    Domaini

    The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.

    Sequence similaritiesi

    Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
    Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0513.
    GeneTreeiENSGT00550000074863.
    HOGENOMiHOG000242486.
    KOiK14779.
    OMAiNVDCLHA.
    OrthoDBiEOG74TX7R.

    Family and domain databases

    Gene3Di3.40.50.300. 2 hits.
    InterProiIPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR027417. P-loop_NTPase.
    IPR000629. RNA-helicase_DEAD-box_CS.
    IPR014014. RNA_helicase_DEAD_Q_motif.
    [Graphical view]
    PfamiPF00270. DEAD. 1 hit.
    PF00271. Helicase_C. 1 hit.
    [Graphical view]
    SMARTiSM00487. DEXDc. 1 hit.
    SM00490. HELICc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 2 hits.
    PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
    PS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    PS51195. Q_MOTIF. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P45818-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDIFRVLTRG ASVKKESGPK AKAADYSVIN GNDENHKEDN NESQIVKELD    50
    FFRNKRIISK VEDDREKTTE NDSPNKEEKS GNDDGLIKPV ITNTVEASAL 100
    RKSYKGNVSG IDIPLPIGSF EDLISRFSFD KRLLNNLIEN GFTEPTPIQC 150
    ECIPVALNNR DVLACGPTGS GKTLAFLIPL VQQIIDDKQT AGLKGLIISP 200
    TKELANQIFI ECFKLSYKIF LEKKRPLQVA LLSKSLGAKL KNKVVSDKKY 250
    DIIISTPLRL IDVVKNEALD LSKVKHLIFD EADKLFDKTF VEQSDDILSA 300
    CREPSLRKAM FSATIPSNVE EIAQSIMMDP VRVIIGHKEA ANTNIEQKLI 350
    FCGNEEGKLI AIRQLVQEGE FKPPIIIFLE SITRAKALYH ELMYDRINVD 400
    VIHAERTALQ RDRIIERFKT GELWCLICTD VLARGIDFKG VNLVINYDVP 450
    GSSQAYVHRI GRTGRGGRSG KAITFYTKQD SVAIKPIINV MKQSGCEVSE 500
    WMDKMAKMTR KEKESIKNGK AHKERKQITT VPKMDKAKRR RQQEMIAASK 550
    RRKNEELSKK HFSK 564
    Length:564
    Mass (Da):63,653
    Last modified:November 1, 1995 - v1
    Checksum:iCACB950B8A15D5A2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z34901 Genomic DNA. Translation: CAA84384.1.
    X85757 Genomic DNA. Translation: CAA59758.1.
    Z72693 Genomic DNA. Translation: CAA96883.1.
    BK006941 Genomic DNA. Translation: DAA07942.1.
    PIRiS59649.
    RefSeqiNP_011344.1. NM_001181036.1.

    Genome annotation databases

    EnsemblFungiiYGL171W; YGL171W; YGL171W.
    GeneIDi852704.
    KEGGisce:YGL171W.

    Cross-referencesi

    Web resourcesi

    RNA helicases from the baker's yeast Saccharomyces cerevisiae

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z34901 Genomic DNA. Translation: CAA84384.1 .
    X85757 Genomic DNA. Translation: CAA59758.1 .
    Z72693 Genomic DNA. Translation: CAA96883.1 .
    BK006941 Genomic DNA. Translation: DAA07942.1 .
    PIRi S59649.
    RefSeqi NP_011344.1. NM_001181036.1.

    3D structure databases

    ProteinModelPortali P45818.
    SMRi P45818. Positions 90-511.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33082. 33 interactions.
    DIPi DIP-5111N.
    IntActi P45818. 16 interactions.
    MINTi MINT-483312.
    STRINGi 4932.YGL171W.

    Proteomic databases

    MaxQBi P45818.
    PaxDbi P45818.
    PeptideAtlasi P45818.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGL171W ; YGL171W ; YGL171W .
    GeneIDi 852704.
    KEGGi sce:YGL171W.

    Organism-specific databases

    CYGDi YGL171w.
    SGDi S000003139. ROK1.

    Phylogenomic databases

    eggNOGi COG0513.
    GeneTreei ENSGT00550000074863.
    HOGENOMi HOG000242486.
    KOi K14779.
    OMAi NVDCLHA.
    OrthoDBi EOG74TX7R.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30659-MONOMER.

    Miscellaneous databases

    NextBioi 972056.
    PROi P45818.

    Gene expression databases

    Genevestigatori P45818.

    Family and domain databases

    Gene3Di 3.40.50.300. 2 hits.
    InterProi IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
    IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR027417. P-loop_NTPase.
    IPR000629. RNA-helicase_DEAD-box_CS.
    IPR014014. RNA_helicase_DEAD_Q_motif.
    [Graphical view ]
    Pfami PF00270. DEAD. 1 hit.
    PF00271. Helicase_C. 1 hit.
    [Graphical view ]
    SMARTi SM00487. DEXDc. 1 hit.
    SM00490. HELICc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 2 hits.
    PROSITEi PS00039. DEAD_ATP_HELICASE. 1 hit.
    PS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    PS51195. Q_MOTIF. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "ROK1, a high-copy-number plasmid suppressor of kem1, encodes a putative ATP-dependent RNA helicase in Saccharomyces cerevisiae."
      Song Y., Kim S., Kim J.
      Gene 166:151-154(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "A putative helicase, the SUA5, PMR1, tRNALys1 genes and four open reading frames have been detected in the DNA sequence of an 8.8 kb fragment of the left arm of chromosome VII of Saccharomyces cerevisiae."
      Klima R., Coglievina M., Zaccaria P., Bertani I., Bruschi C.V.
      Yeast 12:1033-1040(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "Rok1p is a putative RNA helicase required for rRNA processing."
      Venema J., Bousquet-Antonelli C., Gelugne J.-P., Caizergues-Ferrer M., Tollervey D.
      Mol. Cell. Biol. 17:3398-3407(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    6. "Two mutant forms of the S1/TPR-containing protein Rrp5p affect the 18S rRNA synthesis in Saccharomyces cerevisiae."
      Torchet C., Jacq C., Hermann-Le Denmat S.
      RNA 4:1636-1652(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "ATP hydrolysis activity of the DEAD box protein Rok1p is required for in vivo ROK1 function."
      Oh J.Y., Kim J.
      Nucleic Acids Res. 27:2753-2759(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF GLY-166; LYS-172 AND ASP-280.
    8. "Two-hybrid cloning and characterization of OSH3, a yeast oxysterol-binding protein homolog."
      Park Y.-U., Hwang O., Kim J.
      Biochem. Biophys. Res. Commun. 293:733-740(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH OSH3.
    9. "The path from nucleolar 90S to cytoplasmic 40S pre-ribosomes."
      Schaefer T., Strauss D., Petfalski E., Tollervey D., Hurt E.
      EMBO J. 22:1370-1380(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: ASSOCIATION WITH THE 90S AND 40S PRE-RIBOSOMES, IDENTIFICATION BY MASS SPECTROMETRY.
    10. "Deletion of OSH3 gene confers resistance against ISP-1 in Saccharomyces cerevisiae."
      Yano T., Inukai M., Isono F.
      Biochem. Biophys. Res. Commun. 315:228-234(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    11. "Posttranscriptional regulation of the karyogamy gene by Kem1p/Xrn1p exoribonuclease and Rok1p RNA helicase of Saccharomyces cerevisiae."
      Kim J., Jeon S., Yang Y.-S., Kim J.
      Biochem. Biophys. Res. Commun. 321:1032-1039(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    12. "U3 snoRNP and Rrp5p associate independently with Saccharomyces cerevisiae 35S pre-rRNA, but Rrp5p is essential for association of Rok1p."
      Vos H.R., Bax R., Faber A.W., Vos J.C., Raue H.A.
      Nucleic Acids Res. 32:5827-5833(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH THE U3 SNORNA, ASSOCIATION WITH THE PRE-RIBOSOME.

    Entry informationi

    Entry nameiROK1_YEAST
    AccessioniPrimary (citable) accession number: P45818
    Secondary accession number(s): D6VTY1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 133 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3