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P45798 (GUB_RHOMR) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-glucanase

EC=3.2.1.73
Alternative name(s):
1,3-1,4-beta-D-glucan 4-glucanohydrolase
Endo-beta-1,3-1,4 glucanase
Lichenase
Gene names
Name:bglA
OrganismRhodothermus marinus (Rhodothermus obamensis)
Taxonomic identifier29549 [NCBI]
Taxonomic lineageBacteriaBacteroidetesBacteroidetes Order II. Incertae sedisRhodothermaceaeRhodothermus

Protein attributes

Sequence length286 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Shows activity on lichenan, beta-glucan and laminarin but not on CMC cellulose or xylan.

Catalytic activity

Hydrolysis of (1->4)-beta-D-glucosidic linkages in beta-D-glucans containing (1->3)- and (1->4)-bonds.

Sequence similarities

Belongs to the glycosyl hydrolase 16 family.

Biophysicochemical properties

pH dependence:

Optimum pH is 7.0.

Temperature dependence:

Optimum temperature is 85 degrees Celsius.

Ontologies

Keywords
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular_functionlicheninase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3030 Potential
Chain31 – 286256Beta-glucanase
PRO_0000011794

Sites

Active site1581Nucleophile By similarity
Active site1631Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
P45798 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 7215C33624135191

FASTA28633,145
        10         20         30         40         50         60 
MCTMPLMKLK KMMRRTAFLL SVLIGCSMLG SDRSDKAPHW ELVWSDEFDY SGLPDPEKWD 

        70         80         90        100        110        120 
YDVGGHGWGN QELQYYTRAR IENARVGGGV LIIEARHEPY EGREYTSARL VTRGKASWTY 

       130        140        150        160        170        180 
GRFEIRARLP SGRGTWPAIW MLPDRQTYGS AYWPDNGEID IMEHVGFNPD VVHGTVHTKA 

       190        200        210        220        230        240 
YNHLLGTQRG GSIRVPTART DFHVYAIEWT PEEIRWFVDD SLYYRFPNER LTDPEADWRH 

       250        260        270        280 
WPFDQPFHLI MNIAVGGAWG GQQGVDPEAF PAQLVVDYVR VYRWVE 

« Hide

References

[1]"Cloning and sequencing of a Rhodothermus marinus gene, bglA, coding for a thermostable beta-glucanase and its expression in Escherichia coli."
Spilliaert R., Hreggvidsson G.O., Kristjansson J.K., Eggertsson G., Palsdottir A.
Eur. J. Biochem. 224:923-930(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 21 / ITI-378.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U04836 Genomic DNA. Translation: AAA60459.1.
PIRS48201.

3D structure databases

ProteinModelPortalP45798.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH16. Glycoside Hydrolase Family 16.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D2.60.120.200. 1 hit.
InterProIPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR000757. Glyco_hydro_16.
IPR008263. Glycoside_hydrolase_16_AS.
[Graphical view]
PfamPF00722. Glyco_hydro_16. 1 hit.
[Graphical view]
SUPFAMSSF49899. SSF49899. 1 hit.
PROSITEPS01034. GLYCOSYL_HYDROL_F16. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGUB_RHOMR
AccessionPrimary (citable) accession number: P45798
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: October 16, 2013
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries