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P45798

- GUB_RHOMR

UniProt

P45798 - GUB_RHOMR

Protein

Beta-glucanase

Gene

bglA

Organism
Rhodothermus marinus (Rhodothermus obamensis)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 66 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    • Comment

    Functioni

    Shows activity on lichenan, beta-glucan and laminarin but not on CMC cellulose or xylan.

    Catalytic activityi

    Hydrolysis of (1->4)-beta-D-glucosidic linkages in beta-D-glucans containing (1->3)- and (1->4)-bonds.

    pH dependencei

    Optimum pH is 7.0.

    Temperature dependencei

    Optimum temperature is 85 degrees Celsius.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei158 – 1581NucleophilePROSITE-ProRule annotation
    Active sitei163 – 1631Proton donorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. licheninase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Protein family/group databases

    CAZyiGH16. Glycoside Hydrolase Family 16.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-glucanase (EC:3.2.1.73)
    Alternative name(s):
    1,3-1,4-beta-D-glucan 4-glucanohydrolase
    Endo-beta-1,3-1,4 glucanase
    Lichenase
    Gene namesi
    Name:bglA
    OrganismiRhodothermus marinus (Rhodothermus obamensis)
    Taxonomic identifieri29549 [NCBI]
    Taxonomic lineageiBacteriaBacteroidetesBacteroidetes Order II. Incertae sedisRhodothermaceaeRhodothermus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3030Sequence AnalysisAdd
    BLAST
    Chaini31 – 286256Beta-glucanasePRO_0000011794Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP45798.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 16 family.Curated

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di2.60.120.200. 1 hit.
    InterProiIPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR000757. Glyco_hydro_16.
    IPR008263. Glycoside_hydrolase_16_AS.
    [Graphical view]
    PfamiPF00722. Glyco_hydro_16. 1 hit.
    [Graphical view]
    SUPFAMiSSF49899. SSF49899. 1 hit.
    PROSITEiPS01034. GLYCOSYL_HYDROL_F16. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P45798-1 [UniParc]FASTAAdd to Basket

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    MCTMPLMKLK KMMRRTAFLL SVLIGCSMLG SDRSDKAPHW ELVWSDEFDY    50
    SGLPDPEKWD YDVGGHGWGN QELQYYTRAR IENARVGGGV LIIEARHEPY 100
    EGREYTSARL VTRGKASWTY GRFEIRARLP SGRGTWPAIW MLPDRQTYGS 150
    AYWPDNGEID IMEHVGFNPD VVHGTVHTKA YNHLLGTQRG GSIRVPTART 200
    DFHVYAIEWT PEEIRWFVDD SLYYRFPNER LTDPEADWRH WPFDQPFHLI 250
    MNIAVGGAWG GQQGVDPEAF PAQLVVDYVR VYRWVE 286
    Length:286
    Mass (Da):33,145
    Last modified:November 1, 1995 - v1
    Checksum:i7215C33624135191
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U04836 Genomic DNA. Translation: AAA60459.1.
    PIRiS48201.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U04836 Genomic DNA. Translation: AAA60459.1 .
    PIRi S48201.

    3D structure databases

    ProteinModelPortali P45798.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH16. Glycoside Hydrolase Family 16.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 2.60.120.200. 1 hit.
    InterProi IPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR000757. Glyco_hydro_16.
    IPR008263. Glycoside_hydrolase_16_AS.
    [Graphical view ]
    Pfami PF00722. Glyco_hydro_16. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49899. SSF49899. 1 hit.
    PROSITEi PS01034. GLYCOSYL_HYDROL_F16. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and sequencing of a Rhodothermus marinus gene, bglA, coding for a thermostable beta-glucanase and its expression in Escherichia coli."
      Spilliaert R., Hreggvidsson G.O., Kristjansson J.K., Eggertsson G., Palsdottir A.
      Eur. J. Biochem. 224:923-930(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 21 / ITI-378.

    Entry informationi

    Entry nameiGUB_RHOMR
    AccessioniPrimary (citable) accession number: P45798
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 66 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3