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Protein

Beta-glucanase

Gene

bglA

Organism
Rhodothermus marinus (Rhodothermus obamensis)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Shows activity on lichenan, beta-glucan and laminarin but not on CMC cellulose or xylan.

Catalytic activityi

Hydrolysis of (1->4)-beta-D-glucosidic linkages in beta-D-glucans containing (1->3)- and (1->4)-bonds.

pH dependencei

Optimum pH is 7.0.

Temperature dependencei

Optimum temperature is 85 degrees Celsius.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei158 – 1581NucleophilePROSITE-ProRule annotation
Active sitei163 – 1631Proton donorPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH16. Glycoside Hydrolase Family 16.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-glucanase (EC:3.2.1.73)
Alternative name(s):
1,3-1,4-beta-D-glucan 4-glucanohydrolase
Endo-beta-1,3-1,4 glucanase
Lichenase
Gene namesi
Name:bglA
OrganismiRhodothermus marinus (Rhodothermus obamensis)
Taxonomic identifieri29549 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesBacteroidetes Order II. Incertae sedisRhodothermaceaeRhodothermus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3030Sequence AnalysisAdd
BLAST
Chaini31 – 286256Beta-glucanasePRO_0000011794Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi518766.Rmar_0109.

Structurei

3D structure databases

ProteinModelPortaliP45798.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 16 family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR000757. Glyco_hydro_16.
IPR008263. Glycoside_hydrolase_16_AS.
[Graphical view]
PfamiPF00722. Glyco_hydro_16. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS01034. GLYCOSYL_HYDROL_F16. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P45798-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCTMPLMKLK KMMRRTAFLL SVLIGCSMLG SDRSDKAPHW ELVWSDEFDY
60 70 80 90 100
SGLPDPEKWD YDVGGHGWGN QELQYYTRAR IENARVGGGV LIIEARHEPY
110 120 130 140 150
EGREYTSARL VTRGKASWTY GRFEIRARLP SGRGTWPAIW MLPDRQTYGS
160 170 180 190 200
AYWPDNGEID IMEHVGFNPD VVHGTVHTKA YNHLLGTQRG GSIRVPTART
210 220 230 240 250
DFHVYAIEWT PEEIRWFVDD SLYYRFPNER LTDPEADWRH WPFDQPFHLI
260 270 280
MNIAVGGAWG GQQGVDPEAF PAQLVVDYVR VYRWVE
Length:286
Mass (Da):33,145
Last modified:November 1, 1995 - v1
Checksum:i7215C33624135191
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U04836 Genomic DNA. Translation: AAA60459.1.
PIRiS48201.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U04836 Genomic DNA. Translation: AAA60459.1.
PIRiS48201.

3D structure databases

ProteinModelPortaliP45798.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi518766.Rmar_0109.

Protein family/group databases

CAZyiGH16. Glycoside Hydrolase Family 16.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR000757. Glyco_hydro_16.
IPR008263. Glycoside_hydrolase_16_AS.
[Graphical view]
PfamiPF00722. Glyco_hydro_16. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS01034. GLYCOSYL_HYDROL_F16. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and sequencing of a Rhodothermus marinus gene, bglA, coding for a thermostable beta-glucanase and its expression in Escherichia coli."
    Spilliaert R., Hreggvidsson G.O., Kristjansson J.K., Eggertsson G., Palsdottir A.
    Eur. J. Biochem. 224:923-930(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 21 / ITI-378.

Entry informationi

Entry nameiGUB_RHOMR
AccessioniPrimary (citable) accession number: P45798
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: June 24, 2015
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.