P45798 (GUB_RHOMR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-glucanase
EC=3.2.1.73

Alternative name(s):
1,3-1,4-beta-D-glucan 4-glucanohydrolase
Endo-beta-1,3-1,4 glucanase
Lichenase

Gene names

Name:

bglA

Organism

Rhodothermus marinus (Rhodothermus obamensis)

Taxonomic identifier

29549 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Bacteroidetes/Chlorobi group › Bacteroidetes › Bacteroidetes Order II. Incertae sedis › Rhodothermaceae › Rhodothermus

Protein attributes

Sequence length	286 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Shows activity on lichenan, beta-glucan and laminarin but not on CMC cellulose or xylan.
Catalytic activity	Hydrolysis of (1->4)-beta-D-glucosidic linkages in beta-D-glucans containing (1->3)- and (1->4)-bonds.
Sequence similarities	Belongs to the glycosyl hydrolase 16 family.
Biophysicochemical properties	pH dependence: Optimum pH is 7.0. Temperature dependence: Optimum temperature is 85 degrees Celsius.

Ontologies

Keywords
Domain	Signal
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	licheninase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 30	30	Potential
	Chain	31 – 286	256	Beta-glucanase		PRO_0000011794
Sites
	Active site	158	1	Nucleophile By similarity
	Active site	163	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	P45798 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 7215C33624135191	FASTA	286	33,145
	10 20 30 40 50 60 MCTMPLMKLK KMMRRTAFLL SVLIGCSMLG SDRSDKAPHW ELVWSDEFDY SGLPDPEKWD 70 80 90 100 110 120 YDVGGHGWGN QELQYYTRAR IENARVGGGV LIIEARHEPY EGREYTSARL VTRGKASWTY 130 140 150 160 170 180 GRFEIRARLP SGRGTWPAIW MLPDRQTYGS AYWPDNGEID IMEHVGFNPD VVHGTVHTKA 190 200 210 220 230 240 YNHLLGTQRG GSIRVPTART DFHVYAIEWT PEEIRWFVDD SLYYRFPNER LTDPEADWRH 250 260 270 280 WPFDQPFHLI MNIAVGGAWG GQQGVDPEAF PAQLVVDYVR VYRWVE

References

[1]

"Cloning and sequencing of a Rhodothermus marinus gene, bglA, coding for a thermostable beta-glucanase and its expression in Escherichia coli." Spilliaert R., Hreggvidsson G.O., Kristjansson J.K., Eggertsson G., Palsdottir A. Eur. J. Biochem. 224:923-930(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 21 / ITI-378.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U04836 Genomic DNA. Translation: AAA60459.1.
PIR	S48201.
3D structure databases
ProteinModelPortal	P45798.
ModBase	Search...
Protein family/group databases
CAZy	GH16. Glycoside Hydrolase Family 16.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	2.60.120.200. 1 hit.
InterPro	IPR008985. ConA-like_lec_gl_sf. IPR013320. ConA-like_subgrp. IPR000757. Glyco_hydro_16. IPR008263. Glycoside_hydrolase_16_AS. [Graphical view]
Pfam	PF00722. Glyco_hydro_16. 1 hit. [Graphical view]
SUPFAM	SSF49899. ConA_like_lec_gl. 1 hit.
PROSITE	PS01034. GLYCOSYL_HYDROL_F16. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUB_RHOMR

Accession

Primary (citable) accession number: P45798

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	April 3, 2013
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program