Reviewed, UniProtKB/Swiss-Prot P45797 (GUB_PAEPO)

Names and origin

Protein names

Recommended name:
    Beta-glucanase
    EC=3.2.1.73
Alternative name(s):
    Endo-beta-1,3-1,4 glucanase
    1,3-1,4-beta-D-glucan 4-glucanohydrolase
    Lichenase

Gene names

Name:

gluB

Organism

Paenibacillus polymyxa (Bacillus polymyxa)

Taxonomic identifier

1406 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Paenibacillaceae › Paenibacillus

Protein attributes

Sequence length	238 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Hydrolysis of (1->4)-beta-D-glucosidic linkages in beta-D-glucans containing (1->3)- and (1->4)-bonds.
Miscellaneous	Beta-glucanases of Bacillus have a substrate range similar to lichenase of germinating barley.
Sequence similarities	Belongs to the glycosyl hydrolase 16 family.

Ontologies

Keywords
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	licheninase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	Potential
	Chain	27 – 238	212	Beta-glucanase		PRO_0000011790
Sites
	Active site	129	1	Nucleophile By similarity
	Active site	133	1	Proton donor By similarity
Amino acid modifications
	Disulfide bond	56 ↔ 85		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P45797-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: C0CF7B4EA5D40E8C	FASTA	238	26,919
	10 20 30 40 50 60 MMKKKSWFTL MITGVISLFF SVSAFAGNVF WEPLSYFNSS TWQKADGYSN GQMFNCTWRA 70 80 90 100 110 120 NNVNFTNDGK LKLSLTSPAN NKFDCGEYRS TNNYGYGLYE VSMKPAKNTG IVSSFFTYTG 130 140 150 160 170 180 PSHGTQWDEI DIEFLGKDTT KVQFNYYTNG VGGHEKIINL GFDASTSFHT YAFDWQPGYI 190 200 210 220 230 KWYVDGVLKH TATTNIPSTP GKIMMNLWNG TGVDSWLGSY NGANPLYAEY DWVKYTSN

References

[1]

"Two beta-glycanase genes are clustered in Bacillus polymyxa: molecular cloning, expression, and sequence analysis of genes encoding a xylanase and an endo-beta-(1,3)-(1,4)-glucanase." Gosalbes M.J., Perez-Gonzalez J.A., Gonzalez R., Navarro A. J. Bacteriol. 173:7705-7710(1991) [PubMed: 1938968] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 842 / DSM 36 / IFO 15309 / NCIB 8158 / NCTC 10343.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X57094 Genomic DNA. Translation: CAA40379.1.
PIR	S19012.
3D structure databases
HSSP	HSSP built from PDB template 1AXK based on UniProtKB P23904.
SMR	P45797. Positions 27-238.
ModBase	Search...
Protein family/group databases
CAZy	GH16. Glycoside Hydrolase Family 16.
Enzyme and pathway databases
BRENDA	3.2.1.73. 276031.
Family and domain databases
InterPro	IPR008264. Beta_glucanase. IPR013320. ConA-like_subgrp. IPR000757. Glyco_hydro_16. IPR008263. Glycoside_hydrolase_16_AS. [Graphical view]
Gene3D	G3DSA:2.60.120.200. ConA_like_subgrp. 1 hit.
Pfam	PF00722. Glyco_hydro_16. 1 hit. [Graphical view]
PRINTS	PR00737. GLHYDRLASE16.
PROSITE	PS01034. GLYCOSYL_HYDROL_F16. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUB_PAEPO

Accession

Primary (citable) accession number: P45797

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	June 16, 2009
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)