P45773 (GSPG_VIBCH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Type II secretion system protein G
Short name=T2SS protein G

Alternative name(s):
Cholera toxin secretion protein EpsG
General secretion pathway protein G

Gene names

Name:	epsG
Ordered Locus Names:	VC_2730

Organism

Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) [Reference proteome] [HAMAP]

Taxonomic identifier

243277 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio › Vibrio cholerae › Vibrio cholerae O1 › Vibrio cholerae O1 biovar El Tor

Protein attributes

Sequence length	146 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in a type II secretion system (T2SS, formerly general secretion pathway, GSP) for the export of proteins. Required for secretion of cholera toxin through the outer membrane.
Sequence similarities	Belongs to the GSP G family.

Ontologies

Keywords
Biological process	Protein transport Transport
PTM	Methylation
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein secretion Inferred from sequence or structural similarity Ref.2. Source: TIGR protein secretion by the type II secretion system Inferred from electronic annotation. Source: InterPro
Cellular_component	type II protein secretion system complex Inferred from electronic annotation. Source: InterPro
Molecular_function	protein transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Propeptide

1 – 9

9

By similarity

PRO_0000024210

Chain

10 – 146

137

Type II secretion system protein G

PRO_0000024211

Amino acid modifications

Modified residue

10

1

N-methylphenylalanine By similarity

Secondary structure

1

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146

Helix Strand Turn

Details...

Helix

36 – 63

28

Turn

69 – 71

3

Helix

73 – 76

4

Beta strand

93 – 96

4

Beta strand

107 – 111

5

Beta strand

113 – 121

9

Turn

123 – 126

4

Beta strand

127 – 129

3

Helix

132 – 134

3

Helix

139 – 145

7

Sequences

	Sequence		Length	Mass (Da)
	P45773 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 2B211AA994761E2A	FASTA	146	16,080
	10 20 30 40 50 60 MKKMRKQTGF TLLEVMVVVV ILGILASFVV PNLLGNKEKA DQQKAVTDIV ALENALDMYK 70 80 90 100 110 120 LDNSVYPTTD QGLEALVTKP TNPEPRNYRE GGYIKRLPKD PWGNDYQYLS PGDKGTIDVF 130 140 TLGADGQEGG EGTGADIGNW NIQDFQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Organization of the general secretion pathway genes in Vibrio cholerae." Overbye L.J. Thesis (1994), Michigan State University, United States Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: El Tor TRH7000.
[2]	"DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae." Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R., Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D., Vamathevan J.J., Bass S. , Qin H., Dragoi I., Sellers P., McDonald L.A., Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L., Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M. Nature 406:477-483(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 39315 / El Tor Inaba N16961.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

L33796 Genomic DNA. Translation: AAA58788.1.
AE003852 Genomic DNA. Translation: AAF95870.1.

PIR

D82041.

RefSeq

NP_232357.1. NC_002505.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3FU1	X-ray	1.90	A/B	35-146	[»]

ProteinModelPortal

P45773.

SMR

P45773. Positions 35-142.

ModBase

Search...

Protein-protein interaction databases

STRING

243277.VC2730.

Protocols and materials databases

DNASU

2614893.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAF95870; AAF95870; VC_2730.

GeneID

2614893.

KEGG

vch:VC2730.

PATRIC

20084478. VBIVibCho83274_2605.

Phylogenomic databases

eggNOG

COG2165.

KO

K02456.

OMA

PIDPWGN.

ProtClustDB

CLSK874901.

Family and domain databases

InterPro

IPR000983. Bac_GSPG.
IPR012902. N_methyl_site.
IPR013545. T2SS_protein-G.
IPR010054. Type2_sec_GspG.
[Graphical view]

Pfam

PF13544. N_methyl_2. 1 hit.
PF08334. T2SG. 1 hit.
[Graphical view]

PRINTS

PR00813. BCTERIALGSPG.

TIGRFAMs

TIGR02532. IV_pilin_GFxxxE. 1 hit.
TIGR01710. typeII_sec_gspG. 1 hit.

PROSITE

PS00409. PROKAR_NTER_METHYL. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P45773.

Entry information

Entry name

GSPG_VIBCH

Accession

Primary (citable) accession number: P45773
Secondary accession number(s): Q9JPZ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program