P45762 (GSPK_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative type II secretion system protein K
Short name=T2SS protein K

Alternative name(s):
Putative general secretion pathway protein K

Gene names

Name:	gspK
Synonyms:	yheJ
Ordered Locus Names:	b3332, JW3294

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	327 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in a type II secretion system (T2SS, formerly general secretion pathway, GSP) for the export of proteins Potential.
Subcellular location	Cell inner membrane; Single-pass membrane protein Potential.
Induction	Silenced by the DNA-binding protein H-NS under standard growth conditions. Ref.4
Miscellaneous	Part of a cryptic operon that encodes proteins involved in type II secretion machinery in other organisms, but is not expressed in strain K12.
Sequence similarities	Belongs to the GSP K family.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein secretion Inferred from electronic annotation. Source: InterPro
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 327	327	Putative type II secretion system protein K		PRO_0000215007
Regions
	Topological domain	1 – 6	6	Cytoplasmic Potential
	Transmembrane	7 – 27	21	Helical; Potential
	Topological domain	28 – 327	300	Periplasmic Potential

Sequences

	Sequence		Length	Mass (Da)
	P45762 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 6AFD6A3D2DBF2123	FASTA	327	37,647
	10 20 30 40 50 60 MNNEQRGVAL LIVLMLLALM AALAADMTLS FHSQLQRTRQ VNHHLQRQYD IELAEKLALA 70 80 90 100 110 120 SLTQDVKDND RQTTLQQYWA QPQQLQLEDG NTVKWQLRDA QHCFNLNALA KISDDPLASP 130 140 150 160 170 180 DFPAQVFSAL LINAGIDRGN TDEIVQSIAD YIDVDDSPRF HGAEDSFYQS QTPPRHSANQ 190 200 210 220 230 240 MLFLTGELRQ IKGITENIYQ RLIPYVCVLP TTELSINLNM LTENDIPLFR ALFLNNITDA 250 260 270 280 290 300 DARVLLQKRP REGWLTTDAF LYWAQQDFSG VKPLVAQVKR HLFPYSRYFT LSTESISDEQ 310 320 SQGWQSHIFF NRKQQSAQIY RRTLQLY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The cryptic general secretory pathway (gsp) operon of Escherichia coli K-12 encodes functional proteins." Francetic O., Pugsley A.P. J. Bacteriol. 178:3544-3549(1996) [PubMed] [Europe PMC] [Abstract] Cited for: LACK OF EXPRESSION. Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
[4]	"Expression of the endogenous type II secretion pathway in Escherichia coli leads to chitinase secretion." Francetic O., Belin D., Badaut C., Pugsley A.P. EMBO J. 19:6697-6703(2000) [PubMed] [Europe PMC] [Abstract] Cited for: LACK OF EXPRESSION, TRANSCRIPTIONAL REGULATION. Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U18997 Genomic DNA. Translation: AAA58129.1. U00096 Genomic DNA. Translation: AAC76357.1. AP009048 Genomic DNA. Translation: BAE77959.1.
PIR	G65126.
RefSeq	NP_417791.1. NC_000913.2. YP_492100.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P45762.
SMR	P45762. Positions 69-299.
ModBase	Search...
Protein-protein interaction databases
STRING	511145.b3332.
Proteomic databases
PRIDE	P45762.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC76357; AAC76357; b3332. BAE77959; BAE77959; BAE77959.
GeneID	12934438. 947831.
KEGG	ecj:Y75_p3844. eco:b3332.
PATRIC	32122096. VBIEscCol129921_3425.
Organism-specific databases
EchoBASE	EB2731.
EcoGene	EG12894. gspK.
Phylogenomic databases
eggNOG	COG3156.
HOGENOM	HOG000009791.
KO	K02460.
OMA	WRLNANA.
ProtClustDB	CLSK891808.
Enzyme and pathway databases
BioCyc	EcoCyc:G7710-MONOMER. ECOL316407:JW3294-MONOMER.
Gene expression databases
Genevestigator	P45762.
Family and domain databases
InterPro	IPR005628. GspK. [Graphical view]
Pfam	PF03934. T2SK. 1 hit. [Graphical view]
PIRSF	PIRSF002786. XcpX. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GSPK_ECOLI

Accession

Primary (citable) accession number: P45762
Secondary accession number(s): Q2M6Z7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program