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P45759 (GSPE_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Putative type II secretion system protein E

Short name=T2SS protein E
Alternative name(s):
Putative general secretion pathway protein E
Type II traffic warden ATPase
Gene names
Name:gspE
Synonyms:yheG
Ordered Locus Names:b3326, JW3288
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length493 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in a type II secretion system (T2SS, formerly general secretion pathway, GSP) for the export of proteins Potential.

Subcellular location

Cytoplasm Potential.

Induction

Silenced by the DNA-binding protein H-NS under standard growth conditions. Ref.4

Miscellaneous

Part of a cryptic operon that encodes proteins involved in type II secretion machinery in other organisms, but is not expressed in strain K12.

Sequence similarities

Belongs to the GSP E family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

rplBP604222EBI-1132437,EBI-543515

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 493493Putative type II secretion system protein E
PRO_0000207284

Regions

Nucleotide binding249 – 2568ATP Potential

Sequences

Sequence LengthMass (Da)Tools
P45759 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: A13C67C3D48596CA

FASTA49354,611
        10         20         30         40         50         60 
MRIHSPYPAS WALAQRIGYL YSEGEIIYLA DTPFERLLDI QRQVGQCQTM TSLSQADFEA 

        70         80         90        100        110        120 
RLEAVFHQNT GESQQIAQDI DQSVDLLSLS EEMPANEDLL NEDSAAPVIR LINAILSEAI 

       130        140        150        160        170        180 
KETASDIHIE TYEKTMSIRF RIDGVLRTIL QPNKKLAALL ISRIKVMARL DIAEKRIPQD 

       190        200        210        220        230        240 
GRISLRIGRR NIDVRVSTLP SIYGERAVLR LLDKNSLQLS LNNLGMTAAD KQDLENLIQL 

       250        260        270        280        290        300 
PHGIILVTGP TGSGKSTTLY AILSALNTPG RNILTVEDPV EYELEGIGQT QVNTRVDMSF 

       310        320        330        340        350        360 
ARGLRAILRQ DPDVVMVGEI RDTETAQIAV QASLTGHLVL STLHTNSASG AVTRLRDMGV 

       370        380        390        400        410        420 
ESFLLSSSLA GIIAQRLVRR LCPQCRQFTP VSPQQAQMFK YHQLAVTTIG TPVGCPHCHQ 

       430        440        450        460        470        480 
SGYQGRMAIH EMMVVTPELR AAIHENVDEQ ALERLVRQQH KALIKNGLQK VISGDTSWDE 

       490 
VMRVASATLE SEA 

« Hide

References

« Hide 'large scale' references
[1]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[2]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]"The cryptic general secretory pathway (gsp) operon of Escherichia coli K-12 encodes functional proteins."
Francetic O., Pugsley A.P.
J. Bacteriol. 178:3544-3549(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: LACK OF EXPRESSION.
Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
[4]"Expression of the endogenous type II secretion pathway in Escherichia coli leads to chitinase secretion."
Francetic O., Belin D., Badaut C., Pugsley A.P.
EMBO J. 19:6697-6703(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: LACK OF EXPRESSION, TRANSCRIPTIONAL REGULATION.
Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U18997 Genomic DNA. Translation: AAA58123.1.
U00096 Genomic DNA. Translation: AAC76351.1.
AP009048 Genomic DNA. Translation: BAE77965.1.
PIRA65126.
RefSeqNP_417785.1. NC_000913.3.
YP_492106.1. NC_007779.1.

3D structure databases

ProteinModelPortalP45759.
SMRP45759. Positions 106-489.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP45759. 1 interaction.
STRING511145.b3326.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC76351; AAC76351; b3326.
BAE77965; BAE77965; BAE77965.
GeneID12932304.
947823.
KEGGecj:Y75_p3850.
eco:b3326.
PATRIC32122084. VBIEscCol129921_3419.

Organism-specific databases

EchoBASEEB2728.
EcoGeneEG12891. gspE.

Phylogenomic databases

eggNOGCOG2804.
HOGENOMHOG000008427.
KOK02454.
OMACGQTGYQ.
OrthoDBEOG63Z76W.
PhylomeDBP45759.

Enzyme and pathway databases

BioCycEcoCyc:G7704-MONOMER.
ECOL316407:JW3288-MONOMER.

Gene expression databases

GenevestigatorP45759.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR003593. AAA+_ATPase.
IPR027417. P-loop_NTPase.
IPR013369. T2SS_GspE.
IPR001482. T2SS_protein-E.
[Graphical view]
PfamPF00437. T2SE. 1 hit.
[Graphical view]
SMARTSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR02533. type_II_gspE. 1 hit.
PROSITEPS00662. T2SP_E. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP45759.

Entry information

Entry nameGSPE_ECOLI
AccessionPrimary (citable) accession number: P45759
Secondary accession number(s): Q2M6Z1
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: June 11, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene