Reviewed, UniProtKB/Swiss-Prot P45759 (GSPE_ECOLI)

Names and origin

Protein names

Recommended name:
    Probable general secretion pathway protein E
Alternative name(s):
    Type II traffic warden ATPase

Gene names

Name:	gspE
Synonyms:	yheG
Ordered Locus Names:	b3326, JW3288

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	493 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in a general secretion pathway (GSP) for the export of proteins By similarity.
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the GSP E family.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein secretion by the type II secretion system Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell type II protein secretion system complex Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct protein transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
rplB	P60422	1	EBI-1132437,EBI-543515

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 493	493	Probable general secretion pathway protein E		PRO_0000207284
Regions
	Nucleotide binding	249 – 256	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	P45759-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: A13C67C3D48596CA	FASTA	493	54,611
	10 20 30 40 50 60 MRIHSPYPAS WALAQRIGYL YSEGEIIYLA DTPFERLLDI QRQVGQCQTM TSLSQADFEA 70 80 90 100 110 120 RLEAVFHQNT GESQQIAQDI DQSVDLLSLS EEMPANEDLL NEDSAAPVIR LINAILSEAI 130 140 150 160 170 180 KETASDIHIE TYEKTMSIRF RIDGVLRTIL QPNKKLAALL ISRIKVMARL DIAEKRIPQD 190 200 210 220 230 240 GRISLRIGRR NIDVRVSTLP SIYGERAVLR LLDKNSLQLS LNNLGMTAAD KQDLENLIQL 250 260 270 280 290 300 PHGIILVTGP TGSGKSTTLY AILSALNTPG RNILTVEDPV EYELEGIGQT QVNTRVDMSF 310 320 330 340 350 360 ARGLRAILRQ DPDVVMVGEI RDTETAQIAV QASLTGHLVL STLHTNSASG AVTRLRDMGV 370 380 390 400 410 420 ESFLLSSSLA GIIAQRLVRR LCPQCRQFTP VSPQQAQMFK YHQLAVTTIG TPVGCPHCHQ 430 440 450 460 470 480 SGYQGRMAIH EMMVVTPELR AAIHENVDEQ ALERLVRQQH KALIKNGLQK VISGDTSWDE 490 VMRVASATLE SEA

References

[1]

"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.

[2]

"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U18997 Genomic DNA. Translation: AAA58123.1. U00096 Genomic DNA. Translation: AAC76351.1. AP009048 Genomic DNA. Translation: BAE77965.1.
PIR	A65126.
RefSeq	AP_004464.1. NP_417785.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P45759. 1 interaction.
STRING	P45759.
Genome annotation databases
GeneID	947823.
GenomeReviews	Gene locus JW3288 in contig AP009048_GR. Gene locus b3326 in contig U00096_GR.
KEGG	ecj:JW3288. eco:b3326.
Organism-specific databases
EchoBASE	EB2728.
EcoGene	EG12891. gspE.
CMR	Search...
Phylogenomic databases
HOGENOM	P45759.
OMA	GASELAM
Enzyme and pathway databases
BioCyc	EcoCyc:G7704-MON. ECOL168927:B3326-MON.
Gene expression databases
Genevestigator	P45759.
Family and domain databases
InterPro	IPR003593. ATPase_AAA+_core. IPR013369. T2SS_gspE. IPR001482. T2SS_protein-E. [Graphical view]
Pfam	PF00437. GSPII_E. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. [Graphical view]
TIGRFAMs	TIGR02533. type_II_gspE. 1 hit.
PROSITE	PS00662. T2SP_E. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSPE_ECOLI

Accession

Primary (citable) accession number: P45759
Secondary accession number(s): Q2M6Z1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	November 24, 2009
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)