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P45759

- GSPE_ECOLI

UniProt

P45759 - GSPE_ECOLI

Protein

Putative type II secretion system protein E

Gene

gspE

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 106 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Involved in a type II secretion system (T2SS, formerly general secretion pathway, GSP) for the export of proteins.Curated

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi249 – 2568ATPSequence Analysis

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. nucleoside-triphosphatase activity Source: InterPro
    3. protein binding Source: IntAct
    4. protein transporter activity Source: InterPro

    GO - Biological processi

    1. protein secretion by the type II secretion system Source: InterPro

    Keywords - Biological processi

    Protein transport, Transport

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:G7704-MONOMER.
    ECOL316407:JW3288-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Putative type II secretion system protein E
    Short name:
    T2SS protein E
    Alternative name(s):
    Putative general secretion pathway protein E
    Type II traffic warden ATPase
    Gene namesi
    Name:gspE
    Synonyms:yheG
    Ordered Locus Names:b3326, JW3288
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG12891. gspE.

    Subcellular locationi

    Cytoplasm Curated

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. type II protein secretion system complex Source: InterPro

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 493493Putative type II secretion system protein EPRO_0000207284Add
    BLAST

    Expressioni

    Inductioni

    Silenced by the DNA-binding protein H-NS under standard growth conditions.1 Publication

    Gene expression databases

    GenevestigatoriP45759.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    rplBP604222EBI-1132437,EBI-543515

    Protein-protein interaction databases

    IntActiP45759. 1 interaction.
    STRINGi511145.b3326.

    Structurei

    3D structure databases

    ProteinModelPortaliP45759.
    SMRiP45759. Positions 106-489.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the GSP E family.Curated

    Phylogenomic databases

    eggNOGiCOG2804.
    HOGENOMiHOG000008427.
    KOiK02454.
    OMAiCGQTGYQ.
    OrthoDBiEOG63Z76W.
    PhylomeDBiP45759.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR003593. AAA+_ATPase.
    IPR027417. P-loop_NTPase.
    IPR013369. T2SS_GspE.
    IPR001482. T2SS_protein-E.
    [Graphical view]
    PfamiPF00437. T2SE. 1 hit.
    [Graphical view]
    SMARTiSM00382. AAA. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR02533. type_II_gspE. 1 hit.
    PROSITEiPS00662. T2SP_E. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P45759-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRIHSPYPAS WALAQRIGYL YSEGEIIYLA DTPFERLLDI QRQVGQCQTM    50
    TSLSQADFEA RLEAVFHQNT GESQQIAQDI DQSVDLLSLS EEMPANEDLL 100
    NEDSAAPVIR LINAILSEAI KETASDIHIE TYEKTMSIRF RIDGVLRTIL 150
    QPNKKLAALL ISRIKVMARL DIAEKRIPQD GRISLRIGRR NIDVRVSTLP 200
    SIYGERAVLR LLDKNSLQLS LNNLGMTAAD KQDLENLIQL PHGIILVTGP 250
    TGSGKSTTLY AILSALNTPG RNILTVEDPV EYELEGIGQT QVNTRVDMSF 300
    ARGLRAILRQ DPDVVMVGEI RDTETAQIAV QASLTGHLVL STLHTNSASG 350
    AVTRLRDMGV ESFLLSSSLA GIIAQRLVRR LCPQCRQFTP VSPQQAQMFK 400
    YHQLAVTTIG TPVGCPHCHQ SGYQGRMAIH EMMVVTPELR AAIHENVDEQ 450
    ALERLVRQQH KALIKNGLQK VISGDTSWDE VMRVASATLE SEA 493
    Length:493
    Mass (Da):54,611
    Last modified:November 1, 1995 - v1
    Checksum:iA13C67C3D48596CA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U18997 Genomic DNA. Translation: AAA58123.1.
    U00096 Genomic DNA. Translation: AAC76351.1.
    AP009048 Genomic DNA. Translation: BAE77965.1.
    PIRiA65126.
    RefSeqiNP_417785.1. NC_000913.3.
    YP_492106.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC76351; AAC76351; b3326.
    BAE77965; BAE77965; BAE77965.
    GeneIDi12932304.
    947823.
    KEGGiecj:Y75_p3850.
    eco:b3326.
    PATRICi32122084. VBIEscCol129921_3419.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U18997 Genomic DNA. Translation: AAA58123.1 .
    U00096 Genomic DNA. Translation: AAC76351.1 .
    AP009048 Genomic DNA. Translation: BAE77965.1 .
    PIRi A65126.
    RefSeqi NP_417785.1. NC_000913.3.
    YP_492106.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P45759.
    SMRi P45759. Positions 106-489.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P45759. 1 interaction.
    STRINGi 511145.b3326.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC76351 ; AAC76351 ; b3326 .
    BAE77965 ; BAE77965 ; BAE77965 .
    GeneIDi 12932304.
    947823.
    KEGGi ecj:Y75_p3850.
    eco:b3326.
    PATRICi 32122084. VBIEscCol129921_3419.

    Organism-specific databases

    EchoBASEi EB2728.
    EcoGenei EG12891. gspE.

    Phylogenomic databases

    eggNOGi COG2804.
    HOGENOMi HOG000008427.
    KOi K02454.
    OMAi CGQTGYQ.
    OrthoDBi EOG63Z76W.
    PhylomeDBi P45759.

    Enzyme and pathway databases

    BioCyci EcoCyc:G7704-MONOMER.
    ECOL316407:JW3288-MONOMER.

    Miscellaneous databases

    PROi P45759.

    Gene expression databases

    Genevestigatori P45759.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR003593. AAA+_ATPase.
    IPR027417. P-loop_NTPase.
    IPR013369. T2SS_GspE.
    IPR001482. T2SS_protein-E.
    [Graphical view ]
    Pfami PF00437. T2SE. 1 hit.
    [Graphical view ]
    SMARTi SM00382. AAA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR02533. type_II_gspE. 1 hit.
    PROSITEi PS00662. T2SP_E. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    2. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    3. "The cryptic general secretory pathway (gsp) operon of Escherichia coli K-12 encodes functional proteins."
      Francetic O., Pugsley A.P.
      J. Bacteriol. 178:3544-3549(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: LACK OF EXPRESSION.
      Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
    4. "Expression of the endogenous type II secretion pathway in Escherichia coli leads to chitinase secretion."
      Francetic O., Belin D., Badaut C., Pugsley A.P.
      EMBO J. 19:6697-6703(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: LACK OF EXPRESSION, TRANSCRIPTIONAL REGULATION.
      Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.

    Entry informationi

    Entry nameiGSPE_ECOLI
    AccessioniPrimary (citable) accession number: P45759
    Secondary accession number(s): Q2M6Z1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 106 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Part of a cryptic operon that encodes proteins involved in type II secretion machinery in other organisms, but is not expressed in strain K12.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3