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P45759

- GSPE_ECOLI

UniProt

P45759 - GSPE_ECOLI

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Protein
Putative type II secretion system protein E
Gene
gspE, yheG, b3326, JW3288
Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in a type II secretion system (T2SS, formerly general secretion pathway, GSP) for the export of proteins Reviewed prediction.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi249 – 2568ATP Reviewed prediction

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. nucleoside-triphosphatase activity Source: InterPro
  3. protein binding Source: IntAct
  4. protein transporter activity Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. protein secretion by the type II secretion system Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:G7704-MONOMER.
ECOL316407:JW3288-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative type II secretion system protein E
Short name:
T2SS protein E
Alternative name(s):
Putative general secretion pathway protein E
Type II traffic warden ATPase
Gene namesi
Name:gspE
Synonyms:yheG
Ordered Locus Names:b3326, JW3288
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG12891. gspE.

Subcellular locationi

Cytoplasm Reviewed prediction

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. type II protein secretion system complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 493493Putative type II secretion system protein E
PRO_0000207284Add
BLAST

Expressioni

Inductioni

Silenced by the DNA-binding protein H-NS under standard growth conditions.1 Publication

Gene expression databases

GenevestigatoriP45759.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
rplBP604222EBI-1132437,EBI-543515

Protein-protein interaction databases

IntActiP45759. 1 interaction.
STRINGi511145.b3326.

Structurei

3D structure databases

ProteinModelPortaliP45759.
SMRiP45759. Positions 106-489.

Family & Domainsi

Sequence similaritiesi

Belongs to the GSP E family.

Phylogenomic databases

eggNOGiCOG2804.
HOGENOMiHOG000008427.
KOiK02454.
OMAiCGQTGYQ.
OrthoDBiEOG63Z76W.
PhylomeDBiP45759.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR027417. P-loop_NTPase.
IPR013369. T2SS_GspE.
IPR001482. T2SS_protein-E.
[Graphical view]
PfamiPF00437. T2SE. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR02533. type_II_gspE. 1 hit.
PROSITEiPS00662. T2SP_E. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P45759-1 [UniParc]FASTAAdd to Basket

« Hide

MRIHSPYPAS WALAQRIGYL YSEGEIIYLA DTPFERLLDI QRQVGQCQTM    50
TSLSQADFEA RLEAVFHQNT GESQQIAQDI DQSVDLLSLS EEMPANEDLL 100
NEDSAAPVIR LINAILSEAI KETASDIHIE TYEKTMSIRF RIDGVLRTIL 150
QPNKKLAALL ISRIKVMARL DIAEKRIPQD GRISLRIGRR NIDVRVSTLP 200
SIYGERAVLR LLDKNSLQLS LNNLGMTAAD KQDLENLIQL PHGIILVTGP 250
TGSGKSTTLY AILSALNTPG RNILTVEDPV EYELEGIGQT QVNTRVDMSF 300
ARGLRAILRQ DPDVVMVGEI RDTETAQIAV QASLTGHLVL STLHTNSASG 350
AVTRLRDMGV ESFLLSSSLA GIIAQRLVRR LCPQCRQFTP VSPQQAQMFK 400
YHQLAVTTIG TPVGCPHCHQ SGYQGRMAIH EMMVVTPELR AAIHENVDEQ 450
ALERLVRQQH KALIKNGLQK VISGDTSWDE VMRVASATLE SEA 493
Length:493
Mass (Da):54,611
Last modified:November 1, 1995 - v1
Checksum:iA13C67C3D48596CA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U18997 Genomic DNA. Translation: AAA58123.1.
U00096 Genomic DNA. Translation: AAC76351.1.
AP009048 Genomic DNA. Translation: BAE77965.1.
PIRiA65126.
RefSeqiNP_417785.1. NC_000913.3.
YP_492106.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC76351; AAC76351; b3326.
BAE77965; BAE77965; BAE77965.
GeneIDi12932304.
947823.
KEGGiecj:Y75_p3850.
eco:b3326.
PATRICi32122084. VBIEscCol129921_3419.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U18997 Genomic DNA. Translation: AAA58123.1 .
U00096 Genomic DNA. Translation: AAC76351.1 .
AP009048 Genomic DNA. Translation: BAE77965.1 .
PIRi A65126.
RefSeqi NP_417785.1. NC_000913.3.
YP_492106.1. NC_007779.1.

3D structure databases

ProteinModelPortali P45759.
SMRi P45759. Positions 106-489.
ModBasei Search...

Protein-protein interaction databases

IntActi P45759. 1 interaction.
STRINGi 511145.b3326.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC76351 ; AAC76351 ; b3326 .
BAE77965 ; BAE77965 ; BAE77965 .
GeneIDi 12932304.
947823.
KEGGi ecj:Y75_p3850.
eco:b3326.
PATRICi 32122084. VBIEscCol129921_3419.

Organism-specific databases

EchoBASEi EB2728.
EcoGenei EG12891. gspE.

Phylogenomic databases

eggNOGi COG2804.
HOGENOMi HOG000008427.
KOi K02454.
OMAi CGQTGYQ.
OrthoDBi EOG63Z76W.
PhylomeDBi P45759.

Enzyme and pathway databases

BioCyci EcoCyc:G7704-MONOMER.
ECOL316407:JW3288-MONOMER.

Miscellaneous databases

PROi P45759.

Gene expression databases

Genevestigatori P45759.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR003593. AAA+_ATPase.
IPR027417. P-loop_NTPase.
IPR013369. T2SS_GspE.
IPR001482. T2SS_protein-E.
[Graphical view ]
Pfami PF00437. T2SE. 1 hit.
[Graphical view ]
SMARTi SM00382. AAA. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
TIGRFAMsi TIGR02533. type_II_gspE. 1 hit.
PROSITEi PS00662. T2SP_E. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. "The cryptic general secretory pathway (gsp) operon of Escherichia coli K-12 encodes functional proteins."
    Francetic O., Pugsley A.P.
    J. Bacteriol. 178:3544-3549(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: LACK OF EXPRESSION.
    Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
  4. "Expression of the endogenous type II secretion pathway in Escherichia coli leads to chitinase secretion."
    Francetic O., Belin D., Badaut C., Pugsley A.P.
    EMBO J. 19:6697-6703(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: LACK OF EXPRESSION, TRANSCRIPTIONAL REGULATION.
    Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.

Entry informationi

Entry nameiGSPE_ECOLI
AccessioniPrimary (citable) accession number: P45759
Secondary accession number(s): Q2M6Z1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: June 11, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Part of a cryptic operon that encodes proteins involved in type II secretion machinery in other organisms, but is not expressed in strain K12.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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