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Protein

Putative type II secretion system protein E

Gene

gspE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Involved in a type II secretion system (T2SS, formerly general secretion pathway, GSP) for the export of proteins.Curated

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi249 – 2568ATPSequence Analysis

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. protein transporter activity Source: InterPro

GO - Biological processi

  1. protein secretion by the type II secretion system Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:G7704-MONOMER.
ECOL316407:JW3288-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative type II secretion system protein E
Short name:
T2SS protein E
Alternative name(s):
Putative general secretion pathway protein E
Type II traffic warden ATPase
Gene namesi
Name:gspE
Synonyms:yheG
Ordered Locus Names:b3326, JW3288
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG12891. gspE.

Subcellular locationi

Cytoplasm Curated

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. type II protein secretion system complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 493493Putative type II secretion system protein EPRO_0000207284Add
BLAST

Expressioni

Inductioni

Silenced by the DNA-binding protein H-NS under standard growth conditions.1 Publication

Gene expression databases

GenevestigatoriP45759.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
rplBP604222EBI-1132437,EBI-543515

Protein-protein interaction databases

IntActiP45759. 1 interaction.
STRINGi511145.b3326.

Structurei

3D structure databases

ProteinModelPortaliP45759.
SMRiP45759. Positions 106-489.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GSP E family.Curated

Phylogenomic databases

eggNOGiCOG2804.
HOGENOMiHOG000008427.
InParanoidiP45759.
KOiK02454.
OMAiHDIARTE.
OrthoDBiEOG63Z76W.
PhylomeDBiP45759.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR027417. P-loop_NTPase.
IPR013369. T2SS_GspE.
IPR001482. T2SS_protein-E.
[Graphical view]
PfamiPF00437. T2SE. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR02533. type_II_gspE. 1 hit.
PROSITEiPS00662. T2SP_E. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P45759-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRIHSPYPAS WALAQRIGYL YSEGEIIYLA DTPFERLLDI QRQVGQCQTM
60 70 80 90 100
TSLSQADFEA RLEAVFHQNT GESQQIAQDI DQSVDLLSLS EEMPANEDLL
110 120 130 140 150
NEDSAAPVIR LINAILSEAI KETASDIHIE TYEKTMSIRF RIDGVLRTIL
160 170 180 190 200
QPNKKLAALL ISRIKVMARL DIAEKRIPQD GRISLRIGRR NIDVRVSTLP
210 220 230 240 250
SIYGERAVLR LLDKNSLQLS LNNLGMTAAD KQDLENLIQL PHGIILVTGP
260 270 280 290 300
TGSGKSTTLY AILSALNTPG RNILTVEDPV EYELEGIGQT QVNTRVDMSF
310 320 330 340 350
ARGLRAILRQ DPDVVMVGEI RDTETAQIAV QASLTGHLVL STLHTNSASG
360 370 380 390 400
AVTRLRDMGV ESFLLSSSLA GIIAQRLVRR LCPQCRQFTP VSPQQAQMFK
410 420 430 440 450
YHQLAVTTIG TPVGCPHCHQ SGYQGRMAIH EMMVVTPELR AAIHENVDEQ
460 470 480 490
ALERLVRQQH KALIKNGLQK VISGDTSWDE VMRVASATLE SEA
Length:493
Mass (Da):54,611
Last modified:November 1, 1995 - v1
Checksum:iA13C67C3D48596CA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA. Translation: AAA58123.1.
U00096 Genomic DNA. Translation: AAC76351.1.
AP009048 Genomic DNA. Translation: BAE77965.1.
PIRiA65126.
RefSeqiNP_417785.1. NC_000913.3.
YP_492106.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC76351; AAC76351; b3326.
BAE77965; BAE77965; BAE77965.
GeneIDi12932304.
947823.
KEGGiecj:Y75_p3850.
eco:b3326.
PATRICi32122084. VBIEscCol129921_3419.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA. Translation: AAA58123.1.
U00096 Genomic DNA. Translation: AAC76351.1.
AP009048 Genomic DNA. Translation: BAE77965.1.
PIRiA65126.
RefSeqiNP_417785.1. NC_000913.3.
YP_492106.1. NC_007779.1.

3D structure databases

ProteinModelPortaliP45759.
SMRiP45759. Positions 106-489.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP45759. 1 interaction.
STRINGi511145.b3326.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76351; AAC76351; b3326.
BAE77965; BAE77965; BAE77965.
GeneIDi12932304.
947823.
KEGGiecj:Y75_p3850.
eco:b3326.
PATRICi32122084. VBIEscCol129921_3419.

Organism-specific databases

EchoBASEiEB2728.
EcoGeneiEG12891. gspE.

Phylogenomic databases

eggNOGiCOG2804.
HOGENOMiHOG000008427.
InParanoidiP45759.
KOiK02454.
OMAiHDIARTE.
OrthoDBiEOG63Z76W.
PhylomeDBiP45759.

Enzyme and pathway databases

BioCyciEcoCyc:G7704-MONOMER.
ECOL316407:JW3288-MONOMER.

Miscellaneous databases

PROiP45759.

Gene expression databases

GenevestigatoriP45759.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR027417. P-loop_NTPase.
IPR013369. T2SS_GspE.
IPR001482. T2SS_protein-E.
[Graphical view]
PfamiPF00437. T2SE. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR02533. type_II_gspE. 1 hit.
PROSITEiPS00662. T2SP_E. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. "The cryptic general secretory pathway (gsp) operon of Escherichia coli K-12 encodes functional proteins."
    Francetic O., Pugsley A.P.
    J. Bacteriol. 178:3544-3549(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: LACK OF EXPRESSION.
    Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
  4. "Expression of the endogenous type II secretion pathway in Escherichia coli leads to chitinase secretion."
    Francetic O., Belin D., Badaut C., Pugsley A.P.
    EMBO J. 19:6697-6703(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: LACK OF EXPRESSION, TRANSCRIPTIONAL REGULATION.
    Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.

Entry informationi

Entry nameiGSPE_ECOLI
AccessioniPrimary (citable) accession number: P45759
Secondary accession number(s): Q2M6Z1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: January 7, 2015
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Part of a cryptic operon that encodes proteins involved in type II secretion machinery in other organisms, but is not expressed in strain K12.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.