P45759 (GSPE_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative type II secretion system protein E
Short name=T2SS protein E

Alternative name(s):
Putative general secretion pathway protein E
Type II traffic warden ATPase

Gene names

Name:	gspE
Synonyms:	yheG
Ordered Locus Names:	b3326, JW3288

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	493 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in a type II secretion system (T2SS, formerly general secretion pathway, GSP) for the export of proteins Potential.
Subcellular location	Cytoplasm Potential.
Induction	Silenced by the DNA-binding protein H-NS under standard growth conditions. Ref.4
Miscellaneous	Part of a cryptic operon that encodes proteins involved in type II secretion machinery in other organisms, but is not expressed in strain K12.
Sequence similarities	Belongs to the GSP E family.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein secretion by the type II secretion system Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell type II protein secretion system complex Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro protein transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 493	493	Putative type II secretion system protein E		PRO_0000207284
Regions
	Nucleotide binding	249 – 256	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	P45759 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: A13C67C3D48596CA	FASTA	493	54,611
	10 20 30 40 50 60 MRIHSPYPAS WALAQRIGYL YSEGEIIYLA DTPFERLLDI QRQVGQCQTM TSLSQADFEA 70 80 90 100 110 120 RLEAVFHQNT GESQQIAQDI DQSVDLLSLS EEMPANEDLL NEDSAAPVIR LINAILSEAI 130 140 150 160 170 180 KETASDIHIE TYEKTMSIRF RIDGVLRTIL QPNKKLAALL ISRIKVMARL DIAEKRIPQD 190 200 210 220 230 240 GRISLRIGRR NIDVRVSTLP SIYGERAVLR LLDKNSLQLS LNNLGMTAAD KQDLENLIQL 250 260 270 280 290 300 PHGIILVTGP TGSGKSTTLY AILSALNTPG RNILTVEDPV EYELEGIGQT QVNTRVDMSF 310 320 330 340 350 360 ARGLRAILRQ DPDVVMVGEI RDTETAQIAV QASLTGHLVL STLHTNSASG AVTRLRDMGV 370 380 390 400 410 420 ESFLLSSSLA GIIAQRLVRR LCPQCRQFTP VSPQQAQMFK YHQLAVTTIG TPVGCPHCHQ 430 440 450 460 470 480 SGYQGRMAIH EMMVVTPELR AAIHENVDEQ ALERLVRQQH KALIKNGLQK VISGDTSWDE 490 VMRVASATLE SEA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The cryptic general secretory pathway (gsp) operon of Escherichia coli K-12 encodes functional proteins." Francetic O., Pugsley A.P. J. Bacteriol. 178:3544-3549(1996) [PubMed] [Europe PMC] [Abstract] Cited for: LACK OF EXPRESSION. Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
[4]	"Expression of the endogenous type II secretion pathway in Escherichia coli leads to chitinase secretion." Francetic O., Belin D., Badaut C., Pugsley A.P. EMBO J. 19:6697-6703(2000) [PubMed] [Europe PMC] [Abstract] Cited for: LACK OF EXPRESSION, TRANSCRIPTIONAL REGULATION. Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U18997 Genomic DNA. Translation: AAA58123.1. U00096 Genomic DNA. Translation: AAC76351.1. AP009048 Genomic DNA. Translation: BAE77965.1.
PIR	A65126.
RefSeq	NP_417785.1. NC_000913.2. YP_492106.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P45759.
SMR	P45759. Positions 83-483.
ModBase	Search...
Protein-protein interaction databases
IntAct	P45759. 1 interaction.
STRING	511145.b3326.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC76351; AAC76351; b3326. BAE77965; BAE77965; BAE77965.
GeneID	12932304. 947823.
KEGG	ecj:Y75_p3850. eco:b3326.
PATRIC	32122084. VBIEscCol129921_3419.
Organism-specific databases
EchoBASE	EB2728.
EcoGene	EG12891. gspE.
Phylogenomic databases
eggNOG	COG2804.
HOGENOM	HOG000008427.
KO	K02454.
OMA	ENYLRPT.
ProtClustDB	CLSK891803.
Enzyme and pathway databases
BioCyc	EcoCyc:G7704-MONOMER. ECOL316407:JW3288-MONOMER.
Gene expression databases
Genevestigator	P45759.
Family and domain databases
InterPro	IPR003593. AAA+_ATPase. IPR013369. T2SS_GspE. IPR001482. T2SS_protein-E. [Graphical view]
Pfam	PF00437. T2SE. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. [Graphical view]
TIGRFAMs	TIGR02533. type_II_gspE. 1 hit.
PROSITE	PS00662. T2SP_E. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSPE_ECOLI

Accession

Primary (citable) accession number: P45759
Secondary accession number(s): Q2M6Z1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program