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Protein

Putative type II secretion system protein D

Gene

gspD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in a type II secretion system (T2SS, formerly general secretion pathway, GSP) for the export of proteins.Curated

Miscellaneous

Part of a cryptic operon that encodes proteins involved in type II secretion machinery in other organisms, but is not expressed in strain K12.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processProtein transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:G7703-MONOMER
MetaCyc:G7703-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Putative type II secretion system protein D
Short name:
T2SS protein D
Alternative name(s):
Putative general secretion pathway protein D
Gene namesi
Name:gspD
Synonyms:yheF
Ordered Locus Names:b3325, JW5707
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12890 gspD

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cell outer membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_000001309924 – 650Putative type II secretion system protein DAdd BLAST627

Proteomic databases

PaxDbiP45758
PRIDEiP45758

Expressioni

Inductioni

Silenced by the DNA-binding protein H-NS under standard growth conditions.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4261297, 269 interactors
STRINGi316385.ECDH10B_3500

Structurei

Secondary structure

1650
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi126 – 131Combined sources6
Beta strandi133 – 135Combined sources3
Helixi137 – 140Combined sources4
Helixi141 – 148Combined sources8
Beta strandi151 – 153Combined sources3
Beta strandi156 – 159Combined sources4
Beta strandi164 – 169Combined sources6
Helixi171 – 186Combined sources16
Beta strandi193 – 196Combined sources4
Helixi202 – 210Combined sources9
Beta strandi230 – 232Combined sources3
Beta strandi234 – 236Combined sources3
Beta strandi238 – 241Combined sources4
Helixi245 – 257Combined sources13
Beta strandi267 – 272Combined sources6
Beta strandi274 – 276Combined sources3
Helixi278 – 289Combined sources12
Beta strandi312 – 316Combined sources5
Turni317 – 320Combined sources4
Beta strandi321 – 325Combined sources5
Helixi328 – 341Combined sources14
Beta strandi347 – 371Combined sources25
Turni372 – 374Combined sources3
Beta strandi375 – 378Combined sources4
Beta strandi382 – 384Combined sources3
Helixi386 – 399Combined sources14
Helixi409 – 413Combined sources5
Beta strandi417 – 424Combined sources8
Beta strandi427 – 451Combined sources25
Beta strandi456 – 467Combined sources12
Beta strandi482 – 496Combined sources15
Beta strandi499 – 501Combined sources3
Beta strandi504 – 514Combined sources11
Beta strandi516 – 518Combined sources3
Beta strandi526 – 536Combined sources11
Beta strandi543 – 559Combined sources17
Helixi563 – 565Combined sources3
Helixi569 – 572Combined sources4
Beta strandi575 – 594Combined sources20
Helixi597 – 621Combined sources25

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5WQ7electron microscopy3.04A/B/C/D/E/F/G/H/I/J/K/L/M/N/O24-650[»]
ProteinModelPortaliP45758
SMRiP45758
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GSP D family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105GVR Bacteria
COG1450 LUCA
HOGENOMiHOG000253751
InParanoidiP45758
KOiK02453
OMAiKVIYLKY
PhylomeDBiP45758

Family and domain databases

Gene3Di3.30.1370.120, 3 hits
InterProiView protein in InterPro
IPR001775 GspD/PilQ
IPR005644 NolW-like
IPR038591 NolW-like_sf
IPR004846 T2SS/T3SS
IPR013356 T2SS_GspD
IPR004845 T2SS_GspD_CS
PfamiView protein in Pfam
PF00263 Secretin, 1 hit
PF03958 Secretin_N, 3 hits
PRINTSiPR00811 BCTERIALGSPD
TIGRFAMsiTIGR02517 type_II_gspD, 1 hit
PROSITEiView protein in PROSITE
PS00875 T2SP_D, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P45758-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKGLNKITCC LLAALLMPCA GHAENEQYGA NFNNADIRQF VEIVGQHLGK
60 70 80 90 100
TILIDPSVQG TISVRSNDTF SQQEYYQFFL SILDLYGYSV ITLDNGFLKV
110 120 130 140 150
VRSANVKTSP GMIADSSRPG VGDELVTRIV PLENVPARDL APLLRQMMDA
160 170 180 190 200
GSVGNVVHYE PSNVLILTGR ASTINKLIEV IKRVDVIGTE KQQIIHLEYA
210 220 230 240 250
SAEDLAEILN QLISESHGKS QMPALLSAKI VADKRTNSLI ISGPEKARQR
260 270 280 290 300
ITSLLKSLDV EESEEGNTRV YYLKYAKATN LVEVLTGVSE KLKDEKGNAR
310 320 330 340 350
KPSSSGAMDN VAITADEQTN SLVITADQSV QEKLATVIAR LDIRRAQVLV
360 370 380 390 400
EAIIVEVQDG NGLNLGVQWA NKNVGAQQFT NTGLPIFNAA QGVADYKKNG
410 420 430 440 450
GITSANPAWD MFSAYNGMAA GFFNGDWGVL LTALASNNKN DILATPSIVT
460 470 480 490 500
LDNKLASFNV GQDVPVLSGS QTTSGDNVFN TVERKTVGTK LKVTPQVNEG
510 520 530 540 550
DAVLLEIEQE VSSVDSSSNS TLGPTFNTRT IQNAVLVKTG ETVVLGGLLD
560 570 580 590 600
DFSKEQVSKV PLLGDIPLVG QLFRYTSTER AKRNLMVFIR PTIIRDDDVY
610 620 630 640 650
RSLSKEKYTR YRQEQQQRID GKSKALVGSE DLPVLDENTF NSHAPAPSSR
Length:650
Mass (Da):70,698
Last modified:July 11, 2001 - v2
Checksum:i973259A12A7237B2
GO

Sequence cautioni

The sequence AAA58122 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA Translation: AAA58122.1 Different initiation.
U00096 Genomic DNA Translation: AAC76350.2
AP009048 Genomic DNA Translation: BAE77966.1
PIRiH65125
RefSeqiNP_417784.4, NC_000913.3
WP_001326512.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76350; AAC76350; b3325
BAE77966; BAE77966; BAE77966
GeneIDi947822
KEGGiecj:JW5707
eco:b3325
PATRICifig|1411691.4.peg.3406

Similar proteinsi

Entry informationi

Entry nameiGSPD_ECOLI
AccessioniPrimary (citable) accession number: P45758
Secondary accession number(s): Q2M6Z0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: July 11, 2001
Last modified: March 28, 2018
This is version 127 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health