P45758 (GSPD_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative type II secretion system protein D
Short name=T2SS protein D

Alternative name(s):
Putative general secretion pathway protein D

Gene names

Name:	gspD
Synonyms:	yheF
Ordered Locus Names:	b3325, JW5707

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	650 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in a type II secretion system (T2SS, formerly general secretion pathway, GSP) for the export of proteins Potential.
Subcellular location	Cell outer membrane Potential.
Induction	Silenced by the DNA-binding protein H-NS under standard growth conditions. Ref.4
Miscellaneous	Part of a cryptic operon that encodes proteins involved in type II secretion machinery in other organisms, but is not expressed in strain K12.
Sequence similarities	Belongs to the GSP D family.
Sequence caution	The sequence AAA58122.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cell membrane Cell outer membrane Membrane
Domain	Signal
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein secretion by the type II secretion system Inferred from electronic annotation. Source: InterPro
Cellular_component	cell outer membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW type II protein secretion system complex Inferred from electronic annotation. Source: InterPro
Molecular_function	protein transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 650	627	Putative type II secretion system protein D		PRO_0000013099

Sequences

	Sequence		Length	Mass (Da)
	P45758 [UniParc]. Last modified July 11, 2001. Version 2. Checksum: 973259A12A7237B2	FASTA	650	70,698
	10 20 30 40 50 60 MKGLNKITCC LLAALLMPCA GHAENEQYGA NFNNADIRQF VEIVGQHLGK TILIDPSVQG 70 80 90 100 110 120 TISVRSNDTF SQQEYYQFFL SILDLYGYSV ITLDNGFLKV VRSANVKTSP GMIADSSRPG 130 140 150 160 170 180 VGDELVTRIV PLENVPARDL APLLRQMMDA GSVGNVVHYE PSNVLILTGR ASTINKLIEV 190 200 210 220 230 240 IKRVDVIGTE KQQIIHLEYA SAEDLAEILN QLISESHGKS QMPALLSAKI VADKRTNSLI 250 260 270 280 290 300 ISGPEKARQR ITSLLKSLDV EESEEGNTRV YYLKYAKATN LVEVLTGVSE KLKDEKGNAR 310 320 330 340 350 360 KPSSSGAMDN VAITADEQTN SLVITADQSV QEKLATVIAR LDIRRAQVLV EAIIVEVQDG 370 380 390 400 410 420 NGLNLGVQWA NKNVGAQQFT NTGLPIFNAA QGVADYKKNG GITSANPAWD MFSAYNGMAA 430 440 450 460 470 480 GFFNGDWGVL LTALASNNKN DILATPSIVT LDNKLASFNV GQDVPVLSGS QTTSGDNVFN 490 500 510 520 530 540 TVERKTVGTK LKVTPQVNEG DAVLLEIEQE VSSVDSSSNS TLGPTFNTRT IQNAVLVKTG 550 560 570 580 590 600 ETVVLGGLLD DFSKEQVSKV PLLGDIPLVG QLFRYTSTER AKRNLMVFIR PTIIRDDDVY 610 620 630 640 650 RSLSKEKYTR YRQEQQQRID GKSKALVGSE DLPVLDENTF NSHAPAPSSR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The cryptic general secretory pathway (gsp) operon of Escherichia coli K-12 encodes functional proteins." Francetic O., Pugsley A.P. J. Bacteriol. 178:3544-3549(1996) [PubMed] [Europe PMC] [Abstract] Cited for: LACK OF EXPRESSION. Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
[4]	"Expression of the endogenous type II secretion pathway in Escherichia coli leads to chitinase secretion." Francetic O., Belin D., Badaut C., Pugsley A.P. EMBO J. 19:6697-6703(2000) [PubMed] [Europe PMC] [Abstract] Cited for: LACK OF EXPRESSION, TRANSCRIPTIONAL REGULATION. Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U18997 Genomic DNA. Translation: AAA58122.1. Different initiation. U00096 Genomic DNA. Translation: AAC76350.2. AP009048 Genomic DNA. Translation: BAE77966.1.
PIR	H65125.
RefSeq	NP_417784.4. NC_000913.2. YP_492107.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P45758.
SMR	P45758. Positions 28-259.
ModBase	Search...
Protein-protein interaction databases
STRING	511145.b3325.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC76350; AAC76350; b3325. BAE77966; BAE77966; BAE77966.
GeneID	12932303. 947822.
KEGG	ecj:Y75_p3851. eco:b3325.
PATRIC	32122082. VBIEscCol129921_3418.
Organism-specific databases
EchoBASE	EB2727.
EcoGene	EG12890. gspD.
Phylogenomic databases
eggNOG	COG1450.
HOGENOM	HOG000253751.
KO	K02453.
OMA	IAAGFYQ.
ProtClustDB	CLSK908277.
Enzyme and pathway databases
BioCyc	EcoCyc:G7703-MONOMER. ECOL316407:JW5707-MONOMER. MetaCyc:G7703-MONOMER.
Gene expression databases
Genevestigator	P45758.
Family and domain databases
InterPro	IPR001775. GspD/PilQ. IPR005644. NolW-like. IPR004846. T2SS/T3SS. IPR013356. T2SS_GspD. IPR004845. T2SS_GspD_CS. [Graphical view]
Pfam	PF00263. Secretin. 1 hit. PF03958. Secretin_N. 3 hits. [Graphical view]
PRINTS	PR00811. BCTERIALGSPD.
TIGRFAMs	TIGR02517. type_II_gspD. 1 hit.
PROSITE	PS00875. T2SP_D. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSPD_ECOLI

Accession

Primary (citable) accession number: P45758
Secondary accession number(s): Q2M6Z0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	July 11, 2001
Last modified:	May 1, 2013
This is version 101 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program