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Protein

Putative general secretion pathway protein A

Gene

gspA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

May play a regulatory role under conditions of derepressed gsp gene expression.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi26 – 338ATPSequence analysis

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:G7701-MONOMER.
ECOL316407:JW3285-MONOMER.

Protein family/group databases

TCDBi9.B.42.1.3. the exeab (exeab) secretin assembly/export complex.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative general secretion pathway protein A
Gene namesi
Name:gspA
Synonyms:yheD
Ordered Locus Names:b3323, JW3285
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12888. gspA.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei237 – 25721HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • cytosol Source: EcoCyc
  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 489489Putative general secretion pathway protein APRO_0000214994Add
BLAST

Proteomic databases

EPDiP45756.
PaxDbiP45756.
PRIDEiP45756.

Expressioni

Inductioni

Silenced by the DNA-binding protein H-NS under standard growth conditions.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4261295. 258 interactions.
IntActiP45756. 1 interaction.
STRINGi511145.b3323.

Structurei

3D structure databases

ProteinModelPortaliP45756.
SMRiP45756. Positions 17-46, 293-463.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ExeA family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410899B. Bacteria.
ENOG41120C0. LUCA.
HOGENOMiHOG000009790.
InParanoidiP45756.
KOiK02450.
OMAiWFTRHFS.
OrthoDBiEOG6S52GQ.

Family and domain databases

Gene3Di1.10.101.10. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR027417. P-loop_NTPase.
IPR002477. Peptidoglycan-bd-like.
[Graphical view]
PfamiPF13401. AAA_22. 1 hit.
PF01471. PG_binding_1. 1 hit.
[Graphical view]
SUPFAMiSSF47090. SSF47090. 1 hit.
SSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

P45756-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTRREVILS WLCEKRQTWR LCYLLGEAGS GKTWLAQQLQ KDKHRRVITL
60 70 80 90 100
SLVVSWQGKA AWIVTDDNAA EQGCRDSAWT RDEMAGQLLH ALHRTDSRCP
110 120 130 140 150
LIIIENAHLN HRRILDDLQR AISLIPDGQF LLIGRPDRKV ERDFKKQGIE
160 170 180 190 200
LVSIGRLTEH ELKASILEGQ NIDQPDLLLT ARVLKRIALL CRGDRRKLAL
210 220 230 240 250
AGETIRLLQQ AEQTSVFTAK QWRMIYRILG DNRPRKMQLA VVMSGTIIAL
260 270 280 290 300
TCGWLLLSSF TATLPVPAWL IPVTPVVKQD MTKDIAHVVM RDSEALSVLY
310 320 330 340 350
GVWGYEVPAD SAWCDQAVRA GLACKSGNAS LQTLVDQNLP WIASLKVGDK
360 370 380 390 400
KLPVVVVRVG EASVDVLVGQ QTWTLTHKWF ESVWTGDYLL LWKMSPEGES
410 420 430 440 450
TITRDSSEEE ILWLETMLNR ALHISTEPSA EWRPLLVEKI KQFQKSHHLK
460 470 480
TDGVVGFSTL VHLWQVAGES AYLYRDEANI SPETTVKGK
Length:489
Mass (Da):55,298
Last modified:November 1, 1995 - v1
Checksum:i7C7DDB238274E15C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA. Translation: AAA58120.1.
U00096 Genomic DNA. Translation: AAC76348.1.
AP009048 Genomic DNA. Translation: BAE77968.1.
PIRiF65125.
RefSeqiNP_417782.1. NC_000913.3.
WP_000107576.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76348; AAC76348; b3323.
BAE77968; BAE77968; BAE77968.
GeneIDi947825.
KEGGiecj:JW3285.
eco:b3323.
PATRICi32122078. VBIEscCol129921_3416.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA. Translation: AAA58120.1.
U00096 Genomic DNA. Translation: AAC76348.1.
AP009048 Genomic DNA. Translation: BAE77968.1.
PIRiF65125.
RefSeqiNP_417782.1. NC_000913.3.
WP_000107576.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP45756.
SMRiP45756. Positions 17-46, 293-463.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261295. 258 interactions.
IntActiP45756. 1 interaction.
STRINGi511145.b3323.

Protein family/group databases

TCDBi9.B.42.1.3. the exeab (exeab) secretin assembly/export complex.

Proteomic databases

EPDiP45756.
PaxDbiP45756.
PRIDEiP45756.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76348; AAC76348; b3323.
BAE77968; BAE77968; BAE77968.
GeneIDi947825.
KEGGiecj:JW3285.
eco:b3323.
PATRICi32122078. VBIEscCol129921_3416.

Organism-specific databases

EchoBASEiEB2725.
EcoGeneiEG12888. gspA.

Phylogenomic databases

eggNOGiENOG410899B. Bacteria.
ENOG41120C0. LUCA.
HOGENOMiHOG000009790.
InParanoidiP45756.
KOiK02450.
OMAiWFTRHFS.
OrthoDBiEOG6S52GQ.

Enzyme and pathway databases

BioCyciEcoCyc:G7701-MONOMER.
ECOL316407:JW3285-MONOMER.

Miscellaneous databases

PROiP45756.

Family and domain databases

Gene3Di1.10.101.10. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR027417. P-loop_NTPase.
IPR002477. Peptidoglycan-bd-like.
[Graphical view]
PfamiPF13401. AAA_22. 1 hit.
PF01471. PG_binding_1. 1 hit.
[Graphical view]
SUPFAMiSSF47090. SSF47090. 1 hit.
SSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. "The cryptic general secretory pathway (gsp) operon of Escherichia coli K-12 encodes functional proteins."
    Francetic O., Pugsley A.P.
    J. Bacteriol. 178:3544-3549(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: LACK OF EXPRESSION.
    Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
  4. "Expression of the endogenous type II secretion pathway in Escherichia coli leads to chitinase secretion."
    Francetic O., Belin D., Badaut C., Pugsley A.P.
    EMBO J. 19:6697-6703(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: LACK OF EXPRESSION, FUNCTION, TRANSCRIPTIONAL REGULATION.
    Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.

Entry informationi

Entry nameiGSPA_ECOLI
AccessioniPrimary (citable) accession number: P45756
Secondary accession number(s): Q2M6Y8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: July 6, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Part of a cryptic operon that encodes proteins involved in type II secretion machinery in other organisms, but is not expressed in strain K12.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.