Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P45723 (PLC_STAAE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1-phosphatidylinositol phosphodiesterase

EC=4.6.1.13
Alternative name(s):
Phosphatidylinositol diacylglycerol-lyase
Phosphatidylinositol-specific phospholipase C
Short name=PI-PLC
Gene names
Name:plc
Ordered Locus Names:NWMN_0041
OrganismStaphylococcus aureus (strain Newman) [Complete proteome] [HAMAP]
Taxonomic identifier426430 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesStaphylococcus

Protein attributes

Sequence length312 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Cleaves glycosylphosphatidylinositol (GPI) and phosphatidylinositol (PI) anchors but not PI phosphates. Potential virulence factor.

Catalytic activity

1-phosphatidyl-1D-myo-inositol = 1D-myo-inositol 1,2-cyclic phosphate + 1,2-diacyl-sn-glycerol.

Subcellular location

Secreted.

Sequence similarities

Contains 1 PI-PLC X-box domain.

Sequence caution

The sequence BAF66313.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processLipid degradation
Lipid metabolism
Virulence
   Cellular componentSecreted
   DomainSignal
   Molecular functionLyase
   Technical term3D-structure
Complete proteome
Gene Ontology (GO)
   Biological_processlipid catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

pathogenesis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionphosphatidylinositol diacylglycerol-lyase activity

Inferred from electronic annotation. Source: UniProtKB-EC

phosphoric diester hydrolase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2626 Potential
Chain27 – 3122861-phosphatidylinositol phosphodiesterase
PRO_0000024297

Regions

Domain27 – 176150PI-PLC X-box

Sites

Active site401Proton acceptor By similarity
Active site901Proton donor By similarity

Experimental info

Sequence conflict234 – 2363AKA → LKL in AAA16442. Ref.1
Sequence conflict258 – 2603AFN → DL in AAA16442. Ref.1

Secondary structure

........................................................ 312
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P45723 [UniParc].

Last modified January 15, 2008. Version 2.
Checksum: 6E2F1ADB3EE5382D

FASTA31235,255
        10         20         30         40         50         60 
MSGWYHSAHA SDSLSKSPEN WMSKLDDGKH LTEINIPGSH DSGSFTLKDP VKSVWAKTQD 

        70         80         90        100        110        120 
KDYLTQMKSG VRFFDIRGRA SADNMISVHH GMVYLHHELG KFLDDAKYYL SAYPNETIVM 

       130        140        150        160        170        180 
SMKKDYDSDS KVTKTFEEIF REYYYNNPQY QNLFYTGSNA NPTLKETKGK IVLFNRMGGT 

       190        200        210        220        230        240 
YIKSGYGADT SGIQWADNAT FETKINNGSL NLKVQDEYKD YYDKKVEAVK NLLAKAKTDS 

       250        260        270        280        290        300 
NKDNVYVNFL SVASGGSAFN STYNYASHIN PEIAKTIKAN GKARTGWLIV DYAGYTWPGY 

       310 
DDIVSEIIDS NK 

« Hide

References

« Hide 'large scale' references
[1]"Cloning, expression, and mutagenesis of phosphatidylinositol-specific phospholipase C from Staphylococcus aureus: a potential staphylococcal virulence factor."
Daugherty S., Low M.G.
Infect. Immun. 61:5078-5089(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Genome sequence of Staphylococcus aureus strain Newman and comparative analysis of staphylococcal genomes: polymorphism and evolution of two major pathogenicity islands."
Baba T., Bae T., Schneewind O., Takeuchi F., Hiramatsu K.
J. Bacteriol. 190:300-310(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Newman.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L19298 Unassigned DNA. Translation: AAA16442.1.
AP009351 Genomic DNA. Translation: BAF66313.1. Different initiation.
RefSeqYP_001331076.1. NC_009641.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3V16X-ray2.05A11-312[»]
3V18X-ray2.34A12-312[»]
3V1HX-ray1.90A13-312[»]
4F2BX-ray2.16A/B11-312[»]
4F2TX-ray2.30A11-312[»]
4F2UX-ray2.19A11-312[»]
4I8YX-ray2.10A11-312[»]
4I90X-ray1.65A11-312[»]
4I9JX-ray1.85A11-312[»]
4I9MX-ray2.20A11-312[»]
4I9TX-ray2.00A11-312[»]
ProteinModelPortalP45723.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING426430.NWMN_0041.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAF66313; BAF66313; NWMN_0041.
GeneID5329946.
KEGGsae:NWMN_0041.
PATRIC19583404. VBIStaAur133992_0045.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGNOG14688.
HOGENOMHOG000095714.
KOK01771.
OMANHLYINF.
OrthoDBEOG6091B5.

Enzyme and pathway databases

BioCycSAUR426430:GIXC-43-MONOMER.

Family and domain databases

Gene3D3.20.20.190. 1 hit.
InterProIPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR000909. PLipase_C_PInositol-sp_X_dom.
[Graphical view]
PfamPF00388. PI-PLC-X. 1 hit.
[Graphical view]
SMARTSM00148. PLCXc. 1 hit.
[Graphical view]
SUPFAMSSF51695. SSF51695. 1 hit.
PROSITEPS50007. PIPLC_X_DOMAIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePLC_STAAE
AccessionPrimary (citable) accession number: P45723
Secondary accession number(s): A6QD81
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 15, 2008
Last modified: May 14, 2014
This is version 87 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references