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P45723

- PLC_STAAE

UniProt

P45723 - PLC_STAAE

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Protein

1-phosphatidylinositol phosphodiesterase

Gene

plc

Organism
Staphylococcus aureus (strain Newman)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Cleaves glycosylphosphatidylinositol (GPI) and phosphatidylinositol (PI) anchors but not PI phosphates. Potential virulence factor.

Catalytic activityi

1-phosphatidyl-1D-myo-inositol = 1D-myo-inositol 1,2-cyclic phosphate + 1,2-diacyl-sn-glycerol.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei40 – 401Proton acceptorPROSITE-ProRule annotation
Active sitei90 – 901Proton donorPROSITE-ProRule annotation

GO - Molecular functioni

  1. phosphatidylinositol diacylglycerol-lyase activity Source: UniProtKB-EC
  2. phosphoric diester hydrolase activity Source: InterPro

GO - Biological processi

  1. lipid catabolic process Source: UniProtKB-KW
  2. pathogenesis Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Lipid degradation, Lipid metabolism, Virulence

Enzyme and pathway databases

BioCyciSAUR426430:GIXC-43-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
1-phosphatidylinositol phosphodiesterase (EC:4.6.1.13)
Alternative name(s):
Phosphatidylinositol diacylglycerol-lyase
Phosphatidylinositol-specific phospholipase C
Short name:
PI-PLC
Gene namesi
Name:plc
Ordered Locus Names:NWMN_0041
OrganismiStaphylococcus aureus (strain Newman)
Taxonomic identifieri426430 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
ProteomesiUP000006386: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2626Sequence AnalysisAdd
BLAST
Chaini27 – 3122861-phosphatidylinositol phosphodiesterasePRO_0000024297Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi426430.NWMN_0041.

Structurei

Secondary structure

1
312
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi14 – 163Combined sources
Helixi18 – 247Combined sources
Helixi31 – 333Combined sources
Beta strandi36 – 394Combined sources
Helixi42 – 465Combined sources
Helixi50 – 567Combined sources
Helixi63 – 686Combined sources
Beta strandi73 – 8210Combined sources
Beta strandi85 – 906Combined sources
Beta strandi93 – 986Combined sources
Helixi99 – 11214Combined sources
Beta strandi118 – 1247Combined sources
Helixi136 – 1438Combined sources
Turni144 – 1463Combined sources
Helixi148 – 1503Combined sources
Turni151 – 1533Combined sources
Helixi164 – 1674Combined sources
Beta strandi170 – 1756Combined sources
Beta strandi177 – 1793Combined sources
Beta strandi184 – 1863Combined sources
Beta strandi189 – 1913Combined sources
Beta strandi199 – 2057Combined sources
Turni206 – 2094Combined sources
Beta strandi210 – 2167Combined sources
Helixi222 – 23716Combined sources
Beta strandi244 – 2496Combined sources
Beta strandi256 – 2583Combined sources
Beta strandi259 – 2613Combined sources
Helixi262 – 27918Combined sources
Beta strandi287 – 2926Combined sources
Helixi303 – 3097Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3V16X-ray2.05A11-312[»]
3V18X-ray2.34A12-312[»]
3V1HX-ray1.90A13-312[»]
4F2BX-ray2.16A/B11-312[»]
4F2TX-ray2.30A11-312[»]
4F2UX-ray2.19A11-312[»]
4I8YX-ray2.10A11-312[»]
4I90X-ray1.65A11-312[»]
4I9JX-ray1.85A11-312[»]
4I9MX-ray2.20A11-312[»]
4I9TX-ray2.00A11-312[»]
ProteinModelPortaliP45723.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini27 – 176150PI-PLC X-boxPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 PI-PLC X-box domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG14688.
HOGENOMiHOG000095714.
KOiK01771.
OMAiNHLYINF.
OrthoDBiEOG6091B5.

Family and domain databases

Gene3Di3.20.20.190. 1 hit.
InterProiIPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR000909. PLipase_C_PInositol-sp_X_dom.
[Graphical view]
PfamiPF00388. PI-PLC-X. 1 hit.
[Graphical view]
SMARTiSM00148. PLCXc. 1 hit.
[Graphical view]
SUPFAMiSSF51695. SSF51695. 1 hit.
PROSITEiPS50007. PIPLC_X_DOMAIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P45723-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSGWYHSAHA SDSLSKSPEN WMSKLDDGKH LTEINIPGSH DSGSFTLKDP
60 70 80 90 100
VKSVWAKTQD KDYLTQMKSG VRFFDIRGRA SADNMISVHH GMVYLHHELG
110 120 130 140 150
KFLDDAKYYL SAYPNETIVM SMKKDYDSDS KVTKTFEEIF REYYYNNPQY
160 170 180 190 200
QNLFYTGSNA NPTLKETKGK IVLFNRMGGT YIKSGYGADT SGIQWADNAT
210 220 230 240 250
FETKINNGSL NLKVQDEYKD YYDKKVEAVK NLLAKAKTDS NKDNVYVNFL
260 270 280 290 300
SVASGGSAFN STYNYASHIN PEIAKTIKAN GKARTGWLIV DYAGYTWPGY
310
DDIVSEIIDS NK
Length:312
Mass (Da):35,255
Last modified:January 15, 2008 - v2
Checksum:i6E2F1ADB3EE5382D
GO

Sequence cautioni

The sequence BAF66313.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti234 – 2363AKA → LKL in AAA16442. (PubMed:8225585)Curated
Sequence conflicti258 – 2603AFN → DL in AAA16442. (PubMed:8225585)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L19298 Unassigned DNA. Translation: AAA16442.1.
AP009351 Genomic DNA. Translation: BAF66313.1. Different initiation.
RefSeqiYP_001331076.1. NC_009641.1.

Genome annotation databases

EnsemblBacteriaiBAF66313; BAF66313; NWMN_0041.
GeneIDi5329946.
KEGGisae:NWMN_0041.
PATRICi19583404. VBIStaAur133992_0045.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L19298 Unassigned DNA. Translation: AAA16442.1 .
AP009351 Genomic DNA. Translation: BAF66313.1 . Different initiation.
RefSeqi YP_001331076.1. NC_009641.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3V16 X-ray 2.05 A 11-312 [» ]
3V18 X-ray 2.34 A 12-312 [» ]
3V1H X-ray 1.90 A 13-312 [» ]
4F2B X-ray 2.16 A/B 11-312 [» ]
4F2T X-ray 2.30 A 11-312 [» ]
4F2U X-ray 2.19 A 11-312 [» ]
4I8Y X-ray 2.10 A 11-312 [» ]
4I90 X-ray 1.65 A 11-312 [» ]
4I9J X-ray 1.85 A 11-312 [» ]
4I9M X-ray 2.20 A 11-312 [» ]
4I9T X-ray 2.00 A 11-312 [» ]
ProteinModelPortali P45723.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 426430.NWMN_0041.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAF66313 ; BAF66313 ; NWMN_0041 .
GeneIDi 5329946.
KEGGi sae:NWMN_0041.
PATRICi 19583404. VBIStaAur133992_0045.

Phylogenomic databases

eggNOGi NOG14688.
HOGENOMi HOG000095714.
KOi K01771.
OMAi NHLYINF.
OrthoDBi EOG6091B5.

Enzyme and pathway databases

BioCyci SAUR426430:GIXC-43-MONOMER.

Family and domain databases

Gene3Di 3.20.20.190. 1 hit.
InterProi IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR000909. PLipase_C_PInositol-sp_X_dom.
[Graphical view ]
Pfami PF00388. PI-PLC-X. 1 hit.
[Graphical view ]
SMARTi SM00148. PLCXc. 1 hit.
[Graphical view ]
SUPFAMi SSF51695. SSF51695. 1 hit.
PROSITEi PS50007. PIPLC_X_DOMAIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, expression, and mutagenesis of phosphatidylinositol-specific phospholipase C from Staphylococcus aureus: a potential staphylococcal virulence factor."
    Daugherty S., Low M.G.
    Infect. Immun. 61:5078-5089(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Genome sequence of Staphylococcus aureus strain Newman and comparative analysis of staphylococcal genomes: polymorphism and evolution of two major pathogenicity islands."
    Baba T., Bae T., Schneewind O., Takeuchi F., Hiramatsu K.
    J. Bacteriol. 190:300-310(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Newman.

Entry informationi

Entry nameiPLC_STAAE
AccessioniPrimary (citable) accession number: P45723
Secondary accession number(s): A6QD81
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 15, 2008
Last modified: October 29, 2014
This is version 89 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3