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Protein

1-phosphatidylinositol phosphodiesterase

Gene

plc

Organism
Staphylococcus aureus (strain Newman)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Cleaves glycosylphosphatidylinositol (GPI) and phosphatidylinositol (PI) anchors but not PI phosphates. Potential virulence factor.

Catalytic activityi

1-phosphatidyl-1D-myo-inositol = 1D-myo-inositol 1,2-cyclic phosphate + 1,2-diacyl-sn-glycerol.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei40Proton acceptorPROSITE-ProRule annotation1
Active sitei90Proton donorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Lipid degradation, Lipid metabolism, Virulence

Names & Taxonomyi

Protein namesi
Recommended name:
1-phosphatidylinositol phosphodiesterase (EC:4.6.1.13)
Alternative name(s):
Phosphatidylinositol diacylglycerol-lyase
Phosphatidylinositol-specific phospholipase C
Short name:
PI-PLC
Gene namesi
Name:plc
Ordered Locus Names:NWMN_0041
OrganismiStaphylococcus aureus (strain Newman)
Taxonomic identifieri426430 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000006386 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 26Sequence analysisAdd BLAST26
ChainiPRO_000002429727 – 3121-phosphatidylinositol phosphodiesteraseAdd BLAST286

Structurei

Secondary structure

1312
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi14 – 16Combined sources3
Helixi18 – 24Combined sources7
Helixi31 – 33Combined sources3
Beta strandi36 – 39Combined sources4
Helixi42 – 46Combined sources5
Helixi50 – 56Combined sources7
Helixi63 – 68Combined sources6
Beta strandi73 – 82Combined sources10
Beta strandi85 – 90Combined sources6
Beta strandi93 – 98Combined sources6
Helixi99 – 112Combined sources14
Beta strandi118 – 124Combined sources7
Helixi136 – 143Combined sources8
Turni144 – 146Combined sources3
Helixi148 – 150Combined sources3
Turni151 – 153Combined sources3
Helixi164 – 167Combined sources4
Beta strandi170 – 175Combined sources6
Beta strandi177 – 179Combined sources3
Beta strandi184 – 186Combined sources3
Beta strandi189 – 191Combined sources3
Beta strandi199 – 205Combined sources7
Turni206 – 209Combined sources4
Beta strandi210 – 216Combined sources7
Helixi222 – 237Combined sources16
Beta strandi244 – 249Combined sources6
Beta strandi256 – 258Combined sources3
Beta strandi259 – 261Combined sources3
Helixi262 – 279Combined sources18
Beta strandi287 – 292Combined sources6
Helixi303 – 309Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3V16X-ray2.05A11-312[»]
3V18X-ray2.34A12-312[»]
3V1HX-ray1.90A13-312[»]
4F2BX-ray2.16A/B11-312[»]
4F2TX-ray2.30A11-312[»]
4F2UX-ray2.19A11-312[»]
4I8YX-ray2.10A11-312[»]
4I90X-ray1.65A11-312[»]
4I9JX-ray1.85A11-312[»]
4I9MX-ray2.20A11-312[»]
4I9TX-ray2.00A11-312[»]
4RV3X-ray2.00A12-312[»]
4S3GX-ray2.50A11-312[»]
ProteinModelPortaliP45723.
SMRiP45723.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini27 – 176PI-PLC X-boxPROSITE-ProRule annotationAdd BLAST150

Sequence similaritiesi

Contains 1 PI-PLC X-box domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000095714.
KOiK01771.
OMAiLYHSAHA.

Family and domain databases

Gene3Di3.20.20.190. 1 hit.
InterProiIPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR000909. PLipase_C_PInositol-sp_X_dom.
[Graphical view]
PfamiPF00388. PI-PLC-X. 1 hit.
[Graphical view]
SMARTiSM00148. PLCXc. 1 hit.
[Graphical view]
SUPFAMiSSF51695. SSF51695. 1 hit.
PROSITEiPS50007. PIPLC_X_DOMAIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P45723-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGWYHSAHA SDSLSKSPEN WMSKLDDGKH LTEINIPGSH DSGSFTLKDP
60 70 80 90 100
VKSVWAKTQD KDYLTQMKSG VRFFDIRGRA SADNMISVHH GMVYLHHELG
110 120 130 140 150
KFLDDAKYYL SAYPNETIVM SMKKDYDSDS KVTKTFEEIF REYYYNNPQY
160 170 180 190 200
QNLFYTGSNA NPTLKETKGK IVLFNRMGGT YIKSGYGADT SGIQWADNAT
210 220 230 240 250
FETKINNGSL NLKVQDEYKD YYDKKVEAVK NLLAKAKTDS NKDNVYVNFL
260 270 280 290 300
SVASGGSAFN STYNYASHIN PEIAKTIKAN GKARTGWLIV DYAGYTWPGY
310
DDIVSEIIDS NK
Length:312
Mass (Da):35,255
Last modified:January 15, 2008 - v2
Checksum:i6E2F1ADB3EE5382D
GO

Sequence cautioni

The sequence BAF66313 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti234 – 236AKA → LKL in AAA16442 (PubMed:8225585).Curated3
Sequence conflicti258 – 260AFN → DL in AAA16442 (PubMed:8225585).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L19298 Unassigned DNA. Translation: AAA16442.1.
AP009351 Genomic DNA. Translation: BAF66313.1. Different initiation.

Genome annotation databases

EnsemblBacteriaiBAF66313; BAF66313; NWMN_0041.
KEGGisae:NWMN_0041.
PATRICi19583404. VBIStaAur133992_0045.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L19298 Unassigned DNA. Translation: AAA16442.1.
AP009351 Genomic DNA. Translation: BAF66313.1. Different initiation.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3V16X-ray2.05A11-312[»]
3V18X-ray2.34A12-312[»]
3V1HX-ray1.90A13-312[»]
4F2BX-ray2.16A/B11-312[»]
4F2TX-ray2.30A11-312[»]
4F2UX-ray2.19A11-312[»]
4I8YX-ray2.10A11-312[»]
4I90X-ray1.65A11-312[»]
4I9JX-ray1.85A11-312[»]
4I9MX-ray2.20A11-312[»]
4I9TX-ray2.00A11-312[»]
4RV3X-ray2.00A12-312[»]
4S3GX-ray2.50A11-312[»]
ProteinModelPortaliP45723.
SMRiP45723.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAF66313; BAF66313; NWMN_0041.
KEGGisae:NWMN_0041.
PATRICi19583404. VBIStaAur133992_0045.

Phylogenomic databases

HOGENOMiHOG000095714.
KOiK01771.
OMAiLYHSAHA.

Family and domain databases

Gene3Di3.20.20.190. 1 hit.
InterProiIPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR000909. PLipase_C_PInositol-sp_X_dom.
[Graphical view]
PfamiPF00388. PI-PLC-X. 1 hit.
[Graphical view]
SMARTiSM00148. PLCXc. 1 hit.
[Graphical view]
SUPFAMiSSF51695. SSF51695. 1 hit.
PROSITEiPS50007. PIPLC_X_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPLC_STAAE
AccessioniPrimary (citable) accession number: P45723
Secondary accession number(s): A6QD81
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 15, 2008
Last modified: November 2, 2016
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.