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P45723

- PLC_STAAE

UniProt

P45723 - PLC_STAAE

Protein

1-phosphatidylinositol phosphodiesterase

Gene

plc

Organism
Staphylococcus aureus (strain Newman)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 88 (01 Oct 2014)
      Sequence version 2 (15 Jan 2008)
      Previous versions | rss
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    Functioni

    Cleaves glycosylphosphatidylinositol (GPI) and phosphatidylinositol (PI) anchors but not PI phosphates. Potential virulence factor.

    Catalytic activityi

    1-phosphatidyl-1D-myo-inositol = 1D-myo-inositol 1,2-cyclic phosphate + 1,2-diacyl-sn-glycerol.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei40 – 401Proton acceptorPROSITE-ProRule annotation
    Active sitei90 – 901Proton donorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. phosphatidylinositol diacylglycerol-lyase activity Source: UniProtKB-EC
    2. phosphoric diester hydrolase activity Source: InterPro

    GO - Biological processi

    1. lipid catabolic process Source: UniProtKB-KW
    2. pathogenesis Source: UniProtKB-KW

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Lipid degradation, Lipid metabolism, Virulence

    Enzyme and pathway databases

    BioCyciSAUR426430:GIXC-43-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    1-phosphatidylinositol phosphodiesterase (EC:4.6.1.13)
    Alternative name(s):
    Phosphatidylinositol diacylglycerol-lyase
    Phosphatidylinositol-specific phospholipase C
    Short name:
    PI-PLC
    Gene namesi
    Name:plc
    Ordered Locus Names:NWMN_0041
    OrganismiStaphylococcus aureus (strain Newman)
    Taxonomic identifieri426430 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
    ProteomesiUP000006386: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2626Sequence AnalysisAdd
    BLAST
    Chaini27 – 3122861-phosphatidylinositol phosphodiesterasePRO_0000024297Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi426430.NWMN_0041.

    Structurei

    Secondary structure

    1
    312
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi14 – 163
    Helixi18 – 247
    Helixi31 – 333
    Beta strandi36 – 394
    Helixi42 – 465
    Helixi50 – 567
    Helixi63 – 686
    Beta strandi73 – 8210
    Beta strandi85 – 906
    Beta strandi93 – 986
    Helixi99 – 11214
    Beta strandi118 – 1247
    Helixi136 – 1438
    Turni144 – 1463
    Helixi148 – 1503
    Turni151 – 1533
    Helixi164 – 1674
    Beta strandi170 – 1756
    Beta strandi177 – 1793
    Beta strandi184 – 1863
    Beta strandi189 – 1913
    Beta strandi199 – 2057
    Turni206 – 2094
    Beta strandi210 – 2167
    Helixi222 – 23716
    Beta strandi244 – 2496
    Beta strandi256 – 2583
    Beta strandi259 – 2613
    Helixi262 – 27918
    Beta strandi287 – 2926
    Helixi303 – 3097

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3V16X-ray2.05A11-312[»]
    3V18X-ray2.34A12-312[»]
    3V1HX-ray1.90A13-312[»]
    4F2BX-ray2.16A/B11-312[»]
    4F2TX-ray2.30A11-312[»]
    4F2UX-ray2.19A11-312[»]
    4I8YX-ray2.10A11-312[»]
    4I90X-ray1.65A11-312[»]
    4I9JX-ray1.85A11-312[»]
    4I9MX-ray2.20A11-312[»]
    4I9TX-ray2.00A11-312[»]
    ProteinModelPortaliP45723.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini27 – 176150PI-PLC X-boxPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 PI-PLC X-box domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG14688.
    HOGENOMiHOG000095714.
    KOiK01771.
    OMAiNHLYINF.
    OrthoDBiEOG6091B5.

    Family and domain databases

    Gene3Di3.20.20.190. 1 hit.
    InterProiIPR017946. PLC-like_Pdiesterase_TIM-brl.
    IPR000909. PLipase_C_PInositol-sp_X_dom.
    [Graphical view]
    PfamiPF00388. PI-PLC-X. 1 hit.
    [Graphical view]
    SMARTiSM00148. PLCXc. 1 hit.
    [Graphical view]
    SUPFAMiSSF51695. SSF51695. 1 hit.
    PROSITEiPS50007. PIPLC_X_DOMAIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P45723-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSGWYHSAHA SDSLSKSPEN WMSKLDDGKH LTEINIPGSH DSGSFTLKDP    50
    VKSVWAKTQD KDYLTQMKSG VRFFDIRGRA SADNMISVHH GMVYLHHELG 100
    KFLDDAKYYL SAYPNETIVM SMKKDYDSDS KVTKTFEEIF REYYYNNPQY 150
    QNLFYTGSNA NPTLKETKGK IVLFNRMGGT YIKSGYGADT SGIQWADNAT 200
    FETKINNGSL NLKVQDEYKD YYDKKVEAVK NLLAKAKTDS NKDNVYVNFL 250
    SVASGGSAFN STYNYASHIN PEIAKTIKAN GKARTGWLIV DYAGYTWPGY 300
    DDIVSEIIDS NK 312
    Length:312
    Mass (Da):35,255
    Last modified:January 15, 2008 - v2
    Checksum:i6E2F1ADB3EE5382D
    GO

    Sequence cautioni

    The sequence BAF66313.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti234 – 2363AKA → LKL in AAA16442. (PubMed:8225585)Curated
    Sequence conflicti258 – 2603AFN → DL in AAA16442. (PubMed:8225585)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L19298 Unassigned DNA. Translation: AAA16442.1.
    AP009351 Genomic DNA. Translation: BAF66313.1. Different initiation.
    RefSeqiYP_001331076.1. NC_009641.1.

    Genome annotation databases

    EnsemblBacteriaiBAF66313; BAF66313; NWMN_0041.
    GeneIDi5329946.
    KEGGisae:NWMN_0041.
    PATRICi19583404. VBIStaAur133992_0045.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L19298 Unassigned DNA. Translation: AAA16442.1 .
    AP009351 Genomic DNA. Translation: BAF66313.1 . Different initiation.
    RefSeqi YP_001331076.1. NC_009641.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3V16 X-ray 2.05 A 11-312 [» ]
    3V18 X-ray 2.34 A 12-312 [» ]
    3V1H X-ray 1.90 A 13-312 [» ]
    4F2B X-ray 2.16 A/B 11-312 [» ]
    4F2T X-ray 2.30 A 11-312 [» ]
    4F2U X-ray 2.19 A 11-312 [» ]
    4I8Y X-ray 2.10 A 11-312 [» ]
    4I90 X-ray 1.65 A 11-312 [» ]
    4I9J X-ray 1.85 A 11-312 [» ]
    4I9M X-ray 2.20 A 11-312 [» ]
    4I9T X-ray 2.00 A 11-312 [» ]
    ProteinModelPortali P45723.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 426430.NWMN_0041.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAF66313 ; BAF66313 ; NWMN_0041 .
    GeneIDi 5329946.
    KEGGi sae:NWMN_0041.
    PATRICi 19583404. VBIStaAur133992_0045.

    Phylogenomic databases

    eggNOGi NOG14688.
    HOGENOMi HOG000095714.
    KOi K01771.
    OMAi NHLYINF.
    OrthoDBi EOG6091B5.

    Enzyme and pathway databases

    BioCyci SAUR426430:GIXC-43-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.190. 1 hit.
    InterProi IPR017946. PLC-like_Pdiesterase_TIM-brl.
    IPR000909. PLipase_C_PInositol-sp_X_dom.
    [Graphical view ]
    Pfami PF00388. PI-PLC-X. 1 hit.
    [Graphical view ]
    SMARTi SM00148. PLCXc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51695. SSF51695. 1 hit.
    PROSITEi PS50007. PIPLC_X_DOMAIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning, expression, and mutagenesis of phosphatidylinositol-specific phospholipase C from Staphylococcus aureus: a potential staphylococcal virulence factor."
      Daugherty S., Low M.G.
      Infect. Immun. 61:5078-5089(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Genome sequence of Staphylococcus aureus strain Newman and comparative analysis of staphylococcal genomes: polymorphism and evolution of two major pathogenicity islands."
      Baba T., Bae T., Schneewind O., Takeuchi F., Hiramatsu K.
      J. Bacteriol. 190:300-310(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Newman.

    Entry informationi

    Entry nameiPLC_STAAE
    AccessioniPrimary (citable) accession number: P45723
    Secondary accession number(s): A6QD81
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: January 15, 2008
    Last modified: October 1, 2014
    This is version 88 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3