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P45723

- PLC_STAAE

UniProt

P45723 - PLC_STAAE

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Protein
1-phosphatidylinositol phosphodiesterase
Gene
plc, NWMN_0041
Organism
Staphylococcus aureus (strain Newman)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Cleaves glycosylphosphatidylinositol (GPI) and phosphatidylinositol (PI) anchors but not PI phosphates. Potential virulence factor.

Catalytic activityi

1-phosphatidyl-1D-myo-inositol = 1D-myo-inositol 1,2-cyclic phosphate + 1,2-diacyl-sn-glycerol.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei40 – 401Proton acceptor By similarity
Active sitei90 – 901Proton donor By similarity

GO - Molecular functioni

  1. phosphatidylinositol diacylglycerol-lyase activity Source: UniProtKB-EC
  2. phosphoric diester hydrolase activity Source: InterPro

GO - Biological processi

  1. lipid catabolic process Source: UniProtKB-KW
  2. pathogenesis Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Lipid degradation, Lipid metabolism, Virulence

Enzyme and pathway databases

BioCyciSAUR426430:GIXC-43-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
1-phosphatidylinositol phosphodiesterase (EC:4.6.1.13)
Alternative name(s):
Phosphatidylinositol diacylglycerol-lyase
Phosphatidylinositol-specific phospholipase C
Short name:
PI-PLC
Gene namesi
Name:plc
Ordered Locus Names:NWMN_0041
OrganismiStaphylococcus aureus (strain Newman)
Taxonomic identifieri426430 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
ProteomesiUP000006386: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2626 Reviewed prediction
Add
BLAST
Chaini27 – 3122861-phosphatidylinositol phosphodiesterase
PRO_0000024297Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi426430.NWMN_0041.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi14 – 163
Helixi18 – 247
Helixi31 – 333
Beta strandi36 – 394
Helixi42 – 465
Helixi50 – 567
Helixi63 – 686
Beta strandi73 – 8210
Beta strandi85 – 906
Beta strandi93 – 986
Helixi99 – 11214
Beta strandi118 – 1247
Helixi136 – 1438
Turni144 – 1463
Helixi148 – 1503
Turni151 – 1533
Helixi164 – 1674
Beta strandi170 – 1756
Beta strandi177 – 1793
Beta strandi184 – 1863
Beta strandi189 – 1913
Beta strandi199 – 2057
Turni206 – 2094
Beta strandi210 – 2167
Helixi222 – 23716
Beta strandi244 – 2496
Beta strandi256 – 2583
Beta strandi259 – 2613
Helixi262 – 27918
Beta strandi287 – 2926
Helixi303 – 3097

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3V16X-ray2.05A11-312[»]
3V18X-ray2.34A12-312[»]
3V1HX-ray1.90A13-312[»]
4F2BX-ray2.16A/B11-312[»]
4F2TX-ray2.30A11-312[»]
4F2UX-ray2.19A11-312[»]
4I8YX-ray2.10A11-312[»]
4I90X-ray1.65A11-312[»]
4I9JX-ray1.85A11-312[»]
4I9MX-ray2.20A11-312[»]
4I9TX-ray2.00A11-312[»]
ProteinModelPortaliP45723.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini27 – 176150PI-PLC X-box
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG14688.
HOGENOMiHOG000095714.
KOiK01771.
OMAiNHLYINF.
OrthoDBiEOG6091B5.

Family and domain databases

Gene3Di3.20.20.190. 1 hit.
InterProiIPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR000909. PLipase_C_PInositol-sp_X_dom.
[Graphical view]
PfamiPF00388. PI-PLC-X. 1 hit.
[Graphical view]
SMARTiSM00148. PLCXc. 1 hit.
[Graphical view]
SUPFAMiSSF51695. SSF51695. 1 hit.
PROSITEiPS50007. PIPLC_X_DOMAIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P45723-1 [UniParc]FASTAAdd to Basket

« Hide

MSGWYHSAHA SDSLSKSPEN WMSKLDDGKH LTEINIPGSH DSGSFTLKDP    50
VKSVWAKTQD KDYLTQMKSG VRFFDIRGRA SADNMISVHH GMVYLHHELG 100
KFLDDAKYYL SAYPNETIVM SMKKDYDSDS KVTKTFEEIF REYYYNNPQY 150
QNLFYTGSNA NPTLKETKGK IVLFNRMGGT YIKSGYGADT SGIQWADNAT 200
FETKINNGSL NLKVQDEYKD YYDKKVEAVK NLLAKAKTDS NKDNVYVNFL 250
SVASGGSAFN STYNYASHIN PEIAKTIKAN GKARTGWLIV DYAGYTWPGY 300
DDIVSEIIDS NK 312
Length:312
Mass (Da):35,255
Last modified:January 15, 2008 - v2
Checksum:i6E2F1ADB3EE5382D
GO

Sequence cautioni

The sequence BAF66313.1 differs from that shown. Reason: Erroneous initiation.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti234 – 2363AKA → LKL in AAA16442. 1 Publication
Sequence conflicti258 – 2603AFN → DL in AAA16442. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L19298 Unassigned DNA. Translation: AAA16442.1.
AP009351 Genomic DNA. Translation: BAF66313.1. Different initiation.
RefSeqiYP_001331076.1. NC_009641.1.

Genome annotation databases

EnsemblBacteriaiBAF66313; BAF66313; NWMN_0041.
GeneIDi5329946.
KEGGisae:NWMN_0041.
PATRICi19583404. VBIStaAur133992_0045.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L19298 Unassigned DNA. Translation: AAA16442.1 .
AP009351 Genomic DNA. Translation: BAF66313.1 . Different initiation.
RefSeqi YP_001331076.1. NC_009641.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3V16 X-ray 2.05 A 11-312 [» ]
3V18 X-ray 2.34 A 12-312 [» ]
3V1H X-ray 1.90 A 13-312 [» ]
4F2B X-ray 2.16 A/B 11-312 [» ]
4F2T X-ray 2.30 A 11-312 [» ]
4F2U X-ray 2.19 A 11-312 [» ]
4I8Y X-ray 2.10 A 11-312 [» ]
4I90 X-ray 1.65 A 11-312 [» ]
4I9J X-ray 1.85 A 11-312 [» ]
4I9M X-ray 2.20 A 11-312 [» ]
4I9T X-ray 2.00 A 11-312 [» ]
ProteinModelPortali P45723.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 426430.NWMN_0041.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAF66313 ; BAF66313 ; NWMN_0041 .
GeneIDi 5329946.
KEGGi sae:NWMN_0041.
PATRICi 19583404. VBIStaAur133992_0045.

Phylogenomic databases

eggNOGi NOG14688.
HOGENOMi HOG000095714.
KOi K01771.
OMAi NHLYINF.
OrthoDBi EOG6091B5.

Enzyme and pathway databases

BioCyci SAUR426430:GIXC-43-MONOMER.

Family and domain databases

Gene3Di 3.20.20.190. 1 hit.
InterProi IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR000909. PLipase_C_PInositol-sp_X_dom.
[Graphical view ]
Pfami PF00388. PI-PLC-X. 1 hit.
[Graphical view ]
SMARTi SM00148. PLCXc. 1 hit.
[Graphical view ]
SUPFAMi SSF51695. SSF51695. 1 hit.
PROSITEi PS50007. PIPLC_X_DOMAIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, expression, and mutagenesis of phosphatidylinositol-specific phospholipase C from Staphylococcus aureus: a potential staphylococcal virulence factor."
    Daugherty S., Low M.G.
    Infect. Immun. 61:5078-5089(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Genome sequence of Staphylococcus aureus strain Newman and comparative analysis of staphylococcal genomes: polymorphism and evolution of two major pathogenicity islands."
    Baba T., Bae T., Schneewind O., Takeuchi F., Hiramatsu K.
    J. Bacteriol. 190:300-310(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Newman.

Entry informationi

Entry nameiPLC_STAAE
AccessioniPrimary (citable) accession number: P45723
Secondary accession number(s): A6QD81
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 15, 2008
Last modified: May 14, 2014
This is version 87 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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