P45723 (PLC_STAAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1-phosphatidylinositol phosphodiesterase
EC=4.6.1.13

Alternative name(s):
Phosphatidylinositol diacylglycerol-lyase
Phosphatidylinositol-specific phospholipase C
Short name=PI-PLC

Gene names

Name:	plc
Ordered Locus Names:	NWMN_0041

Organism

Staphylococcus aureus (strain Newman) [Complete proteome] [HAMAP]

Taxonomic identifier

426430 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Staphylococcaceae › Staphylococcus › Staphylococcus aureus › Staphylococcus aureus subsp. aureus

Protein attributes

Sequence length	312 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Cleaves glycosylphosphatidylinositol (GPI) and phosphatidylinositol (PI) anchors but not PI phosphates. Potential virulence factor.
Catalytic activity	1-phosphatidyl-1D-myo-inositol = 1D-myo-inositol 1,2-cyclic phosphate + 1,2-diacyl-sn-glycerol.
Subcellular location	Secreted.
Sequence similarities	Contains 1 PI-PLC X-box domain.
Sequence caution	The sequence BAF66313.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Lipid degradation Lipid metabolism Virulence
Cellular component	Secreted
Domain	Signal
Molecular function	Lyase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological_process	lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	phosphatidylinositol diacylglycerol-lyase activity Inferred from electronic annotation. Source: EC phosphoric diester hydrolase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 26

26

Potential

Chain

27 – 312

286

1-phosphatidylinositol phosphodiesterase

PRO_0000024297

Regions

Domain

27 – 176

150

PI-PLC X-box

Sites

Active site

40

1

Proton acceptor By similarity

Active site

90

1

Proton donor By similarity

Experimental info

Sequence conflict

234 – 236

3

AKA → LKL in AAA16442. Ref.1

Sequence conflict

258 – 260

3

AFN → DL in AAA16442. Ref.1

Secondary structure

1

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312

Helix Strand Turn

Details...

Helix

14 – 16

3

Helix

18 – 20

3

Helix

22 – 24

3

Helix

31 – 33

3

Beta strand

36 – 39

4

Helix

42 – 46

5

Helix

50 – 56

7

Helix

63 – 68

6

Beta strand

73 – 82

10

Beta strand

85 – 90

6

Beta strand

93 – 98

6

Helix

99 – 112

14

Beta strand

118 – 124

7

Helix

136 – 143

8

Turn

144 – 146

3

Helix

148 – 150

3

Turn

151 – 153

3

Helix

164 – 167

4

Beta strand

170 – 179

10

Beta strand

184 – 186

3

Beta strand

189 – 191

3

Beta strand

199 – 205

7

Turn

206 – 209

4

Beta strand

210 – 216

7

Helix

222 – 237

16

Beta strand

244 – 249

6

Beta strand

256 – 258

3

Beta strand

259 – 261

3

Helix

262 – 279

18

Beta strand

287 – 292

6

Helix

303 – 310

8

Sequences

	Sequence		Length	Mass (Da)
	P45723 [UniParc]. Last modified January 15, 2008. Version 2. Checksum: 6E2F1ADB3EE5382D	FASTA	312	35,255
	10 20 30 40 50 60 MSGWYHSAHA SDSLSKSPEN WMSKLDDGKH LTEINIPGSH DSGSFTLKDP VKSVWAKTQD 70 80 90 100 110 120 KDYLTQMKSG VRFFDIRGRA SADNMISVHH GMVYLHHELG KFLDDAKYYL SAYPNETIVM 130 140 150 160 170 180 SMKKDYDSDS KVTKTFEEIF REYYYNNPQY QNLFYTGSNA NPTLKETKGK IVLFNRMGGT 190 200 210 220 230 240 YIKSGYGADT SGIQWADNAT FETKINNGSL NLKVQDEYKD YYDKKVEAVK NLLAKAKTDS 250 260 270 280 290 300 NKDNVYVNFL SVASGGSAFN STYNYASHIN PEIAKTIKAN GKARTGWLIV DYAGYTWPGY 310 DDIVSEIIDS NK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, expression, and mutagenesis of phosphatidylinositol-specific phospholipase C from Staphylococcus aureus: a potential staphylococcal virulence factor." Daugherty S., Low M.G. Infect. Immun. 61:5078-5089(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Genome sequence of Staphylococcus aureus strain Newman and comparative analysis of staphylococcal genomes: polymorphism and evolution of two major pathogenicity islands." Baba T., Bae T., Schneewind O., Takeuchi F., Hiramatsu K. J. Bacteriol. 190:300-310(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Newman.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

L19298 Unassigned DNA. Translation: AAA16442.1.
AP009351 Genomic DNA. Translation: BAF66313.1. Different initiation.

RefSeq

YP_001331076.1. NC_009641.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3V16	X-ray	2.05	A	11-312	[»]
3V18	X-ray	2.34	A	12-312	[»]
3V1H	X-ray	1.90	A	13-312	[»]
4F2B	X-ray	2.16	A/B	11-312	[»]
4F2T	X-ray	2.30	A	11-312	[»]
4F2U	X-ray	2.19	A	11-312	[»]

ModBase

Search...

Protein-protein interaction databases

STRING

426430.NWMN_0041.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

BAF66313; BAF66313; NWMN_0041.

GeneID

5329946.

KEGG

sae:NWMN_0041.

PATRIC

19583404. VBIStaAur133992_0045.

Organism-specific databases

CMR

Search...

Phylogenomic databases

eggNOG

NOG14688.

HOGENOM

HOG000095714.

KO

K01771.

OMA

LENWSKW.

ProtClustDB

CLSK884390.

Enzyme and pathway databases

BioCyc

SAUR426430:GIXC-42-MONOMER.

Family and domain databases

Gene3D

3.20.20.190. 1 hit.

InterPro

IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR000909. PLipase_C_PInositol-sp_X_dom.
[Graphical view]

Pfam

PF00388. PI-PLC-X. 1 hit.
[Graphical view]

SMART

SM00148. PLCXc. 1 hit.
[Graphical view]

SUPFAM

SSF51695. PLC-like_Pdiesterase_TIM-brl. 1 hit.

PROSITE

PS50007. PIPLC_X_DOMAIN. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

PLC_STAAE

Accession

Primary (citable) accession number: P45723
Secondary accession number(s): A6QD81

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	January 15, 2008
Last modified:	May 1, 2013
This is version 82 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program