P45701 (MA1A1_RABIT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mannosyl-oligosaccharide 1,2-alpha-mannosidase IA
EC=3.2.1.113

Alternative name(s):
Man(9)-alpha-mannosidase
Mannosidase alpha class 1A member 1
Processing alpha-1,2-mannosidase IA
Short name=Alpha-1,2-mannosidase IA

Gene names

Name:

MAN1A1

Organism

Oryctolagus cuniculus (Rabbit) [Reference proteome]

Taxonomic identifier

9986 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus

Protein attributes

Sequence length	469 AA.
Sequence status	Fragment.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the maturation of Asn-linked oligosaccharides. Progressively trim alpha-1,2-linked mannose residues from Man9GlcNAc2 to produce Man5GlcNAc2.
Catalytic activity	Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man9(GlcNAc)2.
Cofactor	Calcium.
Enzyme regulation	Inhibited by both 1-deoxymannojirimycin and kifunensine By similarity.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein.
Sequence similarities	Belongs to the glycosyl hydrolase 47 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Ligand	Calcium Metal-binding
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	protein glycosylation Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell endoplasmic reticulum-Golgi intermediate compartment Inferred from electronic annotation. Source: Compara integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro mannosyl-oligosaccharide 1,2-alpha-mannosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 469	›469	Mannosyl-oligosaccharide 1,2-alpha-mannosidase IA		PRO_0000210311
Regions
	Topological domain	‹1 – 469	›469	Lumenal Potential
Sites
	Metal binding	449	1	Calcium By similarity
Amino acid modifications
	Glycosylation	329	1	N-linked (GlcNAc...) Potential
	Disulfide bond	292 ↔ 324		By similarity
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	P45701 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: A1B9128144FD0D39	FASTA	469	53,427
	10 20 30 40 50 60 REPADAAVRE KRAKIKEMME HAWNSYKRYA WGLNELKPIT KEGHSSSLFG TIKGATIVDA 70 80 90 100 110 120 LDTLFIMGME SEFQEAKSWI AENLDFNVNA EISVFEVNIR FVGGLLSAYY LSGEEIFRKK 130 140 150 160 170 180 AVELGIKLLP AFHTPSGIPW ALLNIKSGIG RNWPWASGGS SILAEFGTLH LEFMHLSHLS 190 200 210 220 230 240 GNPIFAEKVM NIRKVLNKLE KPEGLYPNYL NPSSGQWGQH HVSIGGLGDS FYEYLLKAWL 250 260 270 280 290 300 MSEKTDLEAK KMYFDAVQAI ETHLIRKSSG GLTYIAEWKG GLLEHKMGHL TCFAGGMFAL 310 320 330 340 350 360 GADGAPEGRA QHYLELGAEI ARTCHESYNR TFMKLGPEAF RFDGGVEAIA TRQNEKYYIL 370 380 390 400 410 420 RPEVVETYMY MWRLTHDPKY RKWAWEAVEA LESHCRVNGG YSGLRDVYFT HEKYDNVQQS 430 440 450 460 FFLAETLKYL YLIFSDDDLL PLEHWIFNTE AHLLPILPTD QKEVEVKVK

References

[1]

"Isolation and expression of murine and rabbit cDNAs encoding an alpha 1,2-mannosidase involved in the processing of asparagine-linked oligosaccharides." Lal A., Schutzbach J.S., Forsee W.T., Neame P.J., Moremen K.W. J. Biol. Chem. 269:9872-9881(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Tissue: Liver.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U04301 mRNA. Translation: AAA17748.1.
UniGene	Ocu.6237.
3D structure databases
ProteinModelPortal	P45701.
SMR	P45701. Positions 1-457.
ModBase	Search...
Protein-protein interaction databases
STRING	9986.ENSOCUP00000005793.
Protein family/group databases
CAZy	GH47. Glycoside Hydrolase Family 47.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
eggNOG	NOG300315.
HOGENOM	HOG000181988.
HOVERGEN	HBG052389.
OrthoDB	EOG4NVZJW.
Enzyme and pathway databases
UniPathway	UPA00378.
Family and domain databases
Gene3D	1.50.10.50. 1 hit.
InterPro	IPR001382. Glyco_hydro_47. [Graphical view]
PANTHER	PTHR11742. PTHR11742. 1 hit.
Pfam	PF01532. Glyco_hydro_47. 1 hit. [Graphical view]
PRINTS	PR00747. GLYHDRLASE47.
SUPFAM	SSF48225. Glyco_hydro_47. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MA1A1_RABIT

Accession

Primary (citable) accession number: P45701

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	April 3, 2013
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program