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P45700

- MA1A1_MOUSE

UniProt

P45700 - MA1A1_MOUSE

Protein

Mannosyl-oligosaccharide 1,2-alpha-mannosidase IA

Gene

Man1a1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Involved in the maturation of Asn-linked oligosaccharides. Progressively trim alpha-1,2-linked mannose residues from Man9GlcNAc2 to produce Man5GlcNAc2.

    Catalytic activityi

    Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man9(GlcNAc)2.

    Cofactori

    Calcium.

    Enzyme regulationi

    Inhibited by both 1-deoxymannojirimycin and kifunensine.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi635 – 6351Calcium

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro
    2. mannosyl-oligosaccharide 1,2-alpha-mannosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. protein glycosylation Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Ligandi

    Calcium, Metal-binding

    Enzyme and pathway databases

    BRENDAi3.2.1.113. 3474.
    ReactomeiREACT_198545. Progressive trimming of alpha-1,2-linked mannose residues from Man9/8/7GlcNAc2 to produce Man5GlcNAc2.
    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiGH47. Glycoside Hydrolase Family 47.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mannosyl-oligosaccharide 1,2-alpha-mannosidase IA (EC:3.2.1.113)
    Alternative name(s):
    Man(9)-alpha-mannosidase
    Short name:
    Man9-mannosidase
    Mannosidase alpha class 1A member 1
    Processing alpha-1,2-mannosidase IA
    Short name:
    Alpha-1,2-mannosidase IA
    Gene namesi
    Name:Man1a1
    Synonyms:Man1a
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 10

    Organism-specific databases

    MGIiMGI:104677. Man1a.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum-Golgi intermediate compartment Source: Ensembl
    2. Golgi membrane Source: MGI
    3. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 655655Mannosyl-oligosaccharide 1,2-alpha-mannosidase IAPRO_0000210309Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi478 ↔ 5101 Publication
    Glycosylationi515 – 5151N-linked (GlcNAc...)1 Publication

    Post-translational modificationi

    N-linked glycan at Asn-515 consists of Man(6)-GlcNAc2.1 Publication

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    MaxQBiP45700.
    PaxDbiP45700.
    PRIDEiP45700.

    PTM databases

    PhosphoSiteiP45700.

    Expressioni

    Gene expression databases

    ArrayExpressiP45700.
    BgeeiP45700.
    CleanExiMM_MAN1A.
    GenevestigatoriP45700.

    Interactioni

    Protein-protein interaction databases

    IntActiP45700. 1 interaction.
    MINTiMINT-1858603.

    Structurei

    Secondary structure

    1
    655
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi192 – 21625
    Beta strandi219 – 2235
    Turni224 – 2274
    Beta strandi228 – 2303
    Helixi233 – 2353
    Helixi241 – 25212
    Helixi256 – 26914
    Beta strandi275 – 2795
    Helixi280 – 29819
    Helixi301 – 31414
    Helixi315 – 3184
    Beta strandi320 – 3234
    Beta strandi327 – 3304
    Turni331 – 3333
    Helixi343 – 3453
    Helixi349 – 3524
    Helixi356 – 36611
    Helixi370 – 38415
    Helixi388 – 3903
    Beta strandi394 – 3963
    Turni398 – 4003
    Turni412 – 4143
    Helixi415 – 42713
    Turni428 – 4303
    Helixi433 – 44917
    Beta strandi451 – 4533
    Beta strandi459 – 4613
    Beta strandi463 – 4653
    Beta strandi468 – 4703
    Beta strandi472 – 4743
    Helixi476 – 4794
    Helixi480 – 4867
    Helixi496 – 51520
    Beta strandi517 – 5215
    Beta strandi524 – 5296
    Beta strandi533 – 5353
    Helixi539 – 5413
    Helixi549 – 56113
    Helixi564 – 58017
    Beta strandi595 – 5973
    Helixi606 – 6105
    Helixi612 – 6198
    Turni628 – 6303
    Beta strandi631 – 6333
    Beta strandi639 – 6413

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1NXCX-ray1.51A178-655[»]
    ProteinModelPortaliP45700.
    SMRiP45700. Positions 178-644.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP45700.

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 4343CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini65 – 655591LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei44 – 6421Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 47 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG300315.
    HOGENOMiHOG000181988.
    HOVERGENiHBG052389.
    InParanoidiP45700.
    KOiK01230.
    OMAiMAQHYLE.
    OrthoDBiEOG7X0VH1.
    PhylomeDBiP45700.
    TreeFamiTF313420.

    Family and domain databases

    Gene3Di1.50.10.50. 1 hit.
    InterProiIPR001382. Glyco_hydro_47.
    [Graphical view]
    PANTHERiPTHR11742. PTHR11742. 1 hit.
    PfamiPF01532. Glyco_hydro_47. 1 hit.
    [Graphical view]
    PRINTSiPR00747. GLYHDRLASE47.
    SUPFAMiSSF48225. SSF48225. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P45700-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPVGGLLPLF SSPGGGGLGS GLGGGLGGGR KGSGPAAFRL TEKFVLLLVF    50
    SAFITLCFGA IFFLPDSSKL LSGVLFHSNP ALQPPAEHKP GLGARAEDAA 100
    EGRVRHREEG APGDPGAGLE DNLARIRENH ERALREAKET LQKLPEEIQR 150
    DILLEKEKVA QDQLRDKDLF RGLPKVDFLP PVGVENREPA DATIREKRAK 200
    IKEMMTHAWN NYKRYAWGLN ELKPISKEGH SSSLFGNIKG ATIVDALDTL 250
    FIMGMKTEFQ EAKSWIKKYL DFNVNAEVSV FEVNIRFVGG LLSAYYLSGE 300
    EIFRKKAVEL GVKLLPAFHT PSGIPWALLN MKSGIGRNWP WASGGSSILA 350
    EFGTLHLEFM HLSHLSGDPV FAEKVMKIRT VLNKLDKPEG LYPNYLNPSS 400
    GQWGQHHVSV GGLGDSFYEY LLKAWLMSDK TDLEAKKMYF DAVQAIETHL 450
    IRKSSGGLTY IAEWKGGLLE HKMGHLTCFA GGMFALGADG APEARAQHYL 500
    ELGAEIARTC HESYNRTYVK LGPEAFRFDG GVEAIATRQN EKYYILRPEV 550
    IETYMYMWRL THDPKYRTWA WEAVEALESH CRVNGGYSGL RDVYIARESY 600
    DDVQQSFFLA ETLKYLYLIF SDDDLLPLEH WIFNTEAHPF PILREQKKEI 650
    DGKEK 655
    Length:655
    Mass (Da):73,276
    Last modified:November 1, 1995 - v1
    Checksum:i15AF658364930BF4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U04299 mRNA. Translation: AAA17747.1.
    BC015265 mRNA. Translation: AAH15265.1.
    CCDSiCCDS23848.1.
    PIRiA54408.
    RefSeqiNP_032574.1. NM_008548.4.
    XP_006512632.1. XM_006512569.1.
    UniGeneiMm.117294.

    Genome annotation databases

    EnsembliENSMUST00000003843; ENSMUSP00000003843; ENSMUSG00000003746.
    ENSMUST00000105470; ENSMUSP00000101110; ENSMUSG00000003746.
    GeneIDi17155.
    KEGGimmu:17155.
    UCSCiuc007fbw.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U04299 mRNA. Translation: AAA17747.1 .
    BC015265 mRNA. Translation: AAH15265.1 .
    CCDSi CCDS23848.1.
    PIRi A54408.
    RefSeqi NP_032574.1. NM_008548.4.
    XP_006512632.1. XM_006512569.1.
    UniGenei Mm.117294.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1NXC X-ray 1.51 A 178-655 [» ]
    ProteinModelPortali P45700.
    SMRi P45700. Positions 178-644.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P45700. 1 interaction.
    MINTi MINT-1858603.

    Protein family/group databases

    CAZyi GH47. Glycoside Hydrolase Family 47.

    PTM databases

    PhosphoSitei P45700.

    Proteomic databases

    MaxQBi P45700.
    PaxDbi P45700.
    PRIDEi P45700.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000003843 ; ENSMUSP00000003843 ; ENSMUSG00000003746 .
    ENSMUST00000105470 ; ENSMUSP00000101110 ; ENSMUSG00000003746 .
    GeneIDi 17155.
    KEGGi mmu:17155.
    UCSCi uc007fbw.1. mouse.

    Organism-specific databases

    CTDi 17155.
    MGIi MGI:104677. Man1a.

    Phylogenomic databases

    eggNOGi NOG300315.
    HOGENOMi HOG000181988.
    HOVERGENi HBG052389.
    InParanoidi P45700.
    KOi K01230.
    OMAi MAQHYLE.
    OrthoDBi EOG7X0VH1.
    PhylomeDBi P45700.
    TreeFami TF313420.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .
    BRENDAi 3.2.1.113. 3474.
    Reactomei REACT_198545. Progressive trimming of alpha-1,2-linked mannose residues from Man9/8/7GlcNAc2 to produce Man5GlcNAc2.

    Miscellaneous databases

    EvolutionaryTracei P45700.
    NextBioi 291418.
    PROi P45700.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P45700.
    Bgeei P45700.
    CleanExi MM_MAN1A.
    Genevestigatori P45700.

    Family and domain databases

    Gene3Di 1.50.10.50. 1 hit.
    InterProi IPR001382. Glyco_hydro_47.
    [Graphical view ]
    PANTHERi PTHR11742. PTHR11742. 1 hit.
    Pfami PF01532. Glyco_hydro_47. 1 hit.
    [Graphical view ]
    PRINTSi PR00747. GLYHDRLASE47.
    SUPFAMi SSF48225. SSF48225. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and expression of murine and rabbit cDNAs encoding an alpha 1,2-mannosidase involved in the processing of asparagine-linked oligosaccharides."
      Lal A., Schutzbach J.S., Forsee W.T., Neame P.J., Moremen K.W.
      J. Biol. Chem. 269:9872-9881(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BALB/c.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Kidney.
    3. Lubec G., Sunyer B., Chen W.-Q.
      Submitted (JAN-2009) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 424-437, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: OF1.
      Tissue: Hippocampus.
    4. "Structure of mouse Golgi alpha-mannosidase IA reveals the molecular basis for substrate specificity among class 1 (family 47 glycosylhydrolase) alpha1,2-mannosidases."
      Tempel W., Karaveg K., Liu Z.-J., Rose J., Wang B.C., Moremen K.W.
      J. Biol. Chem. 279:29774-29786(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.51 ANGSTROMS) OF 178-655, DISULFIDE BOND, CALCIUM-BINDING SITE, GLYCOSYLATION AT ASN-515.

    Entry informationi

    Entry nameiMA1A1_MOUSE
    AccessioniPrimary (citable) accession number: P45700
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 123 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    3. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    4. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3