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Protein

Mannosyl-oligosaccharide 1,2-alpha-mannosidase IA

Gene

Man1a1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the maturation of Asn-linked oligosaccharides. Progressively trim alpha-1,2-linked mannose residues from Man9GlcNAc2 to produce Man5GlcNAc2.

Catalytic activityi

Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man9(GlcNAc)2.By similarity

Cofactori

Ca2+1 Publication

Enzyme regulationi

Inhibited by both 1-deoxymannojirimycin and kifunensine.By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.By similarity
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei524Proton donorBy similarity1
Metal bindingi635CalciumCombined sources1 Publication1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
LigandCalcium, Metal-binding

Enzyme and pathway databases

BRENDAi3.2.1.113 3474
ReactomeiR-MMU-6811438 Intra-Golgi traffic
R-MMU-964827 Progressive trimming of alpha-1,2-linked mannose residues from Man9/8/7GlcNAc2 to produce Man5GlcNAc2
UniPathwayiUPA00378

Protein family/group databases

CAZyiGH47 Glycoside Hydrolase Family 47

Names & Taxonomyi

Protein namesi
Recommended name:
Mannosyl-oligosaccharide 1,2-alpha-mannosidase IA (EC:3.2.1.113By similarity)
Alternative name(s):
Man(9)-alpha-mannosidase
Short name:
Man9-mannosidase
Mannosidase alpha class 1A member 1
Processing alpha-1,2-mannosidase IA
Short name:
Alpha-1,2-mannosidase IA
Gene namesi
Name:Man1a1
Synonyms:Man1a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:104677 Man1a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 43CytoplasmicSequence analysisAdd BLAST43
Transmembranei44 – 64Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini65 – 655LumenalSequence analysisAdd BLAST591

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002103091 – 655Mannosyl-oligosaccharide 1,2-alpha-mannosidase IAAdd BLAST655

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi478 ↔ 510Combined sources1 Publication
Glycosylationi515N-linked (GlcNAc...) asparagineCombined sources1 Publication1

Post-translational modificationi

N-linked glycan at Asn-515 consists of Man6-GlcNAc2.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiP45700
PaxDbiP45700
PeptideAtlasiP45700
PRIDEiP45700

PTM databases

iPTMnetiP45700
PhosphoSitePlusiP45700

Expressioni

Gene expression databases

BgeeiENSMUSG00000003746
CleanExiMM_MAN1A
ExpressionAtlasiP45700 baseline and differential
GenevisibleiP45700 MM

Interactioni

Protein-protein interaction databases

IntActiP45700, 1 interactor
MINTiP45700
STRINGi10090.ENSMUSP00000003843

Structurei

Secondary structure

1655
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi192 – 216Combined sources25
Beta strandi219 – 223Combined sources5
Turni224 – 227Combined sources4
Beta strandi228 – 230Combined sources3
Helixi233 – 235Combined sources3
Helixi241 – 252Combined sources12
Helixi256 – 269Combined sources14
Beta strandi275 – 279Combined sources5
Helixi280 – 298Combined sources19
Helixi301 – 314Combined sources14
Helixi315 – 318Combined sources4
Beta strandi320 – 323Combined sources4
Beta strandi327 – 330Combined sources4
Turni331 – 333Combined sources3
Beta strandi335 – 337Combined sources3
Helixi343 – 345Combined sources3
Helixi349 – 352Combined sources4
Helixi356 – 366Combined sources11
Helixi370 – 384Combined sources15
Helixi388 – 390Combined sources3
Beta strandi394 – 396Combined sources3
Turni398 – 400Combined sources3
Turni412 – 414Combined sources3
Helixi415 – 427Combined sources13
Turni428 – 430Combined sources3
Helixi433 – 449Combined sources17
Beta strandi451 – 453Combined sources3
Beta strandi459 – 461Combined sources3
Beta strandi463 – 465Combined sources3
Beta strandi468 – 470Combined sources3
Beta strandi472 – 474Combined sources3
Helixi476 – 479Combined sources4
Helixi480 – 486Combined sources7
Turni487 – 490Combined sources4
Helixi496 – 515Combined sources20
Beta strandi517 – 521Combined sources5
Beta strandi524 – 529Combined sources6
Beta strandi533 – 535Combined sources3
Helixi539 – 541Combined sources3
Helixi549 – 561Combined sources13
Helixi564 – 580Combined sources17
Beta strandi595 – 597Combined sources3
Helixi606 – 610Combined sources5
Helixi612 – 619Combined sources8
Turni628 – 630Combined sources3
Beta strandi631 – 633Combined sources3
Beta strandi639 – 641Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1NXCX-ray1.51A178-655[»]
5KKBX-ray1.77A/B176-644[»]
ProteinModelPortaliP45700
SMRiP45700
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP45700

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 47 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2431 Eukaryota
ENOG410XP04 LUCA
GeneTreeiENSGT00390000016529
HOGENOMiHOG000181988
HOVERGENiHBG052389
InParanoidiP45700
KOiK01230
OMAiDHKPGPG
OrthoDBiEOG091G04C8
PhylomeDBiP45700
TreeFamiTF313420

Family and domain databases

Gene3Di1.50.10.10, 1 hit
InterProiView protein in InterPro
IPR012341 6hp_glycosidase-like_sf
IPR001382 Glyco_hydro_47
IPR036026 Seven-hairpin_glycosidases
PfamiView protein in Pfam
PF01532 Glyco_hydro_47, 1 hit
PRINTSiPR00747 GLYHDRLASE47
SUPFAMiSSF48225 SSF48225, 1 hit

Sequencei

Sequence statusi: Complete.

P45700-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPVGGLLPLF SSPGGGGLGS GLGGGLGGGR KGSGPAAFRL TEKFVLLLVF
60 70 80 90 100
SAFITLCFGA IFFLPDSSKL LSGVLFHSNP ALQPPAEHKP GLGARAEDAA
110 120 130 140 150
EGRVRHREEG APGDPGAGLE DNLARIRENH ERALREAKET LQKLPEEIQR
160 170 180 190 200
DILLEKEKVA QDQLRDKDLF RGLPKVDFLP PVGVENREPA DATIREKRAK
210 220 230 240 250
IKEMMTHAWN NYKRYAWGLN ELKPISKEGH SSSLFGNIKG ATIVDALDTL
260 270 280 290 300
FIMGMKTEFQ EAKSWIKKYL DFNVNAEVSV FEVNIRFVGG LLSAYYLSGE
310 320 330 340 350
EIFRKKAVEL GVKLLPAFHT PSGIPWALLN MKSGIGRNWP WASGGSSILA
360 370 380 390 400
EFGTLHLEFM HLSHLSGDPV FAEKVMKIRT VLNKLDKPEG LYPNYLNPSS
410 420 430 440 450
GQWGQHHVSV GGLGDSFYEY LLKAWLMSDK TDLEAKKMYF DAVQAIETHL
460 470 480 490 500
IRKSSGGLTY IAEWKGGLLE HKMGHLTCFA GGMFALGADG APEARAQHYL
510 520 530 540 550
ELGAEIARTC HESYNRTYVK LGPEAFRFDG GVEAIATRQN EKYYILRPEV
560 570 580 590 600
IETYMYMWRL THDPKYRTWA WEAVEALESH CRVNGGYSGL RDVYIARESY
610 620 630 640 650
DDVQQSFFLA ETLKYLYLIF SDDDLLPLEH WIFNTEAHPF PILREQKKEI

DGKEK
Length:655
Mass (Da):73,276
Last modified:November 1, 1995 - v1
Checksum:i15AF658364930BF4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U04299 mRNA Translation: AAA17747.1
BC015265 mRNA Translation: AAH15265.1
CCDSiCCDS23848.1
PIRiA54408
RefSeqiNP_032574.1, NM_008548.4
XP_006512632.1, XM_006512569.3
UniGeneiMm.117294

Genome annotation databases

EnsembliENSMUST00000003843; ENSMUSP00000003843; ENSMUSG00000003746
ENSMUST00000105470; ENSMUSP00000101110; ENSMUSG00000003746
GeneIDi17155
KEGGimmu:17155
UCSCiuc007fbw.1 mouse

Similar proteinsi

Entry informationi

Entry nameiMA1A1_MOUSE
AccessioniPrimary (citable) accession number: P45700
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: March 28, 2018
This is version 153 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

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