Reviewed, UniProtKB/Swiss-Prot P45699 (GUNK_FUSOX)

Names and origin

Protein names	Recommended name:     Putative endoglucanase type K     EC=3.2.1.4 Alternative name(s):     Endo-1,4-beta-glucanase     Cellulase
Organism	Fusarium oxysporum
Taxonomic identifier	5507 [NCBI]
Taxonomic lineage	Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Hypocreomycetidae › Hypocreales › mitosporic Hypocreales › Fusarium › Fusarium oxysporum species complex

Protein attributes

Sequence length	376 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Sequence similarities	Belongs to the glycosyl hydrolase 45 (cellulase K) family. Contains 1 CBM1 (fungal-type carbohydrate-binding) domain.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Domain	Signal
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: InterPro
Molecular function	cellulase activity Inferred from electronic annotation. Source: EC cellulose binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Potential
	Chain	19 – 376	358	Putative endoglucanase type K		PRO_0000008024
Regions
	Domain	335 – 374	40	CBM1
	Region	19 – 308	290	Catalytic
	Region	309 – 338	30	Linker
Sites
	Active site	29	1	Nucleophile By similarity
	Active site	140	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	P45699-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: B430A5F962B9F882	FASTA	376	39,236
	10 20 30 40 50 60 MRSYTLLALA GPLAVSAASG SGHSTRYWDC CKPSCSWSGK AAVNAPALTC DKNDNPISNT 70 80 90 100 110 120 NAVNGCEGGG SAYACTNYSP WAVNDELAYG FAATKISGGS EASWCCACYA LTFTTGPVKG 130 140 150 160 170 180 KKMIVQSTNT GGDLGDNHFD LMMPGGGVGI FDGCTSEFGK ALGGAQYGGI SSRSECDSYP 190 200 210 220 230 240 ELLKDGCHWR FDWFENADNP DFTFEQVQCP KALLDISGCK RDDDSSFPAF KGDTSASKPQ 250 260 270 280 290 300 PSSSAKKTTS AAAAAQPQKT KDSAPVVQKS STKPAAQPEP TKPADKPQTD KPVATKPAAT 310 320 330 340 350 360 KPAQPVNKPK TTQKVRGTKT RGSCPAKTDA TAKASVVPAY YQCGGSKSAY PNGNLACATG 370 SKCVKQNEYY SQCVPN

References

[1]

"The use of conserved cellulase family-specific sequences to clone cellulase homologue cDNAs from Fusarium oxysporum." Sheppard P.O., Grant F.J., Oort P.J., Sprecher C.A., Foster D.C., Hagen F.S., Upshall A., McKnight G.L., O'Hara P.J. Gene 150:163-167(1994) [PubMed: 7959045] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	L29381 mRNA. Translation: AAA65589.1.
3D structure databases
HSSP	HSSP built from PDB template 2ENG based on UniProtKB P43316.
ModBase	Search...
Protein family/group databases
CAZy	CBM1. Carbohydrate-Binding Module Family 1. GH45. Glycoside Hydrolase Family 45.
Enzyme and pathway databases
BRENDA	3.2.1.4. 15244.
Family and domain databases
InterPro	IPR014733. Barwin-like_endoglucanase. IPR000254. CBD_fun. IPR000334. Glyco_hydro_45. [Graphical view]
Gene3D	G3DSA:2.40.40.10. Barwin. 1 hit.
Pfam	PF00734. CBM_1. 1 hit. PF02015. Glyco_hydro_45. 1 hit. [Graphical view]
SMART	SM00236. fCBD. 1 hit. [Graphical view]
PROSITE	PS00562. CBM1_1. 1 hit. PS51164. CBM1_2. 1 hit. PS01140. GLYCOSYL_HYDROL_F45. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUNK_FUSOX

Accession

Primary (citable) accession number: P45699

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	June 16, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)