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P45699 (GUNK_FUSOX) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Putative endoglucanase type K

EC=3.2.1.4
Alternative name(s):
Cellulase
Endo-1,4-beta-glucanase
OrganismFusarium oxysporum (Fusarium vascular wilt)
Taxonomic identifier5507 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesHypocreomycetidaeHypocrealesNectriaceaemitosporic NectriaceaeFusariumFusarium oxysporum species complex

Protein attributes

Sequence length376 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Sequence similarities

Belongs to the glycosyl hydrolase 45 (cellulase K) family.

Contains 1 CBM1 (fungal-type carbohydrate-binding) domain.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
Cellulose degradation
Polysaccharide degradation
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
Gene Ontology (GO)
   Biological_processcellulose catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: InterPro

   Molecular_functioncellulase activity

Inferred from electronic annotation. Source: UniProtKB-EC

cellulose binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1818 Potential
Chain19 – 376358Putative endoglucanase type K
PRO_0000008024

Regions

Domain335 – 37440CBM1
Region19 – 308290Catalytic
Region309 – 33830Linker

Sites

Active site291Nucleophile By similarity
Active site1401Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
P45699 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: B430A5F962B9F882

FASTA37639,236
        10         20         30         40         50         60 
MRSYTLLALA GPLAVSAASG SGHSTRYWDC CKPSCSWSGK AAVNAPALTC DKNDNPISNT 

        70         80         90        100        110        120 
NAVNGCEGGG SAYACTNYSP WAVNDELAYG FAATKISGGS EASWCCACYA LTFTTGPVKG 

       130        140        150        160        170        180 
KKMIVQSTNT GGDLGDNHFD LMMPGGGVGI FDGCTSEFGK ALGGAQYGGI SSRSECDSYP 

       190        200        210        220        230        240 
ELLKDGCHWR FDWFENADNP DFTFEQVQCP KALLDISGCK RDDDSSFPAF KGDTSASKPQ 

       250        260        270        280        290        300 
PSSSAKKTTS AAAAAQPQKT KDSAPVVQKS STKPAAQPEP TKPADKPQTD KPVATKPAAT 

       310        320        330        340        350        360 
KPAQPVNKPK TTQKVRGTKT RGSCPAKTDA TAKASVVPAY YQCGGSKSAY PNGNLACATG 

       370 
SKCVKQNEYY SQCVPN 

« Hide

References

[1]"The use of conserved cellulase family-specific sequences to clone cellulase homologue cDNAs from Fusarium oxysporum."
Sheppard P.O., Grant F.J., Oort P.J., Sprecher C.A., Foster D.C., Hagen F.S., Upshall A., McKnight G.L., O'Hara P.J.
Gene 150:163-167(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L29381 mRNA. Translation: AAA65589.1.

3D structure databases

ProteinModelPortalP45699.
SMRP45699. Positions 20-227.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyCBM1. Carbohydrate-Binding Module Family 1.
GH45. Glycoside Hydrolase Family 45.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D2.40.40.10. 1 hit.
InterProIPR014733. Barwin-like_endoglucanase.
IPR000254. Cellulose-bd_dom_fun.
IPR000334. Glyco_hydro_45.
IPR009009. RlpA-like_DPBB.
[Graphical view]
PfamPF00734. CBM_1. 1 hit.
PF02015. Glyco_hydro_45. 1 hit.
[Graphical view]
SMARTSM00236. fCBD. 1 hit.
[Graphical view]
SUPFAMSSF50685. SSF50685. 1 hit.
SSF57180. SSF57180. 1 hit.
PROSITEPS00562. CBM1_1. 1 hit.
PS51164. CBM1_2. 1 hit.
PS01140. GLYCOSYL_HYDROL_F45. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGUNK_FUSOX
AccessionPrimary (citable) accession number: P45699
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: October 16, 2013
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries