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Protein

Alpha-like toxin BmK-M1

Gene
N/A
Organism
Mesobuthus martensii (Manchurian scorpion) (Buthus martensii)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This alpha-like toxin binds voltage-dependently sodium channels and inhibits the inactivation of the activated channels, thereby blocking neuronal transmission. This toxin is active against mammals and insects. Is active on Nav1.4/SCN4A and Nav1.5/SCN5A. Acts as a cardiotoxin. Is 6-fold more toxic than BmK-M2.2 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ion channel impairing toxin, Neurotoxin, Toxin, Voltage-gated sodium channel impairing toxin

Protein family/group databases

TCDBi8.B.1.1.1. the long (4c-c) scorpion toxin (l-st) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-like toxin BmK-M1
Alternative name(s):
BmK I
BmK-I
Short name:
BmKI
BmK1
Bmk M1
Short name:
BmKM1
OrganismiMesobuthus martensii (Manchurian scorpion) (Buthus martensii)
Taxonomic identifieri34649 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaScorpionesButhidaButhoideaButhidaeMesobuthus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Toxic dosei

LD50 is 0.75 mg/kg by intravenous injection into mice.1 Publication
LD50 is 0.53 mg/kg by intravenous injection into tail mice.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi24Y → F: 25-fold decrease in toxicity to mice. 2 Publications1
Mutagenesisi24Y → G: Complete loss of toxicity to mice, and 70-fold decrease in the binding affinity for insect sodium channels. 2 Publications1
Mutagenesisi27 – 28KP → DS: 43.4-fold decrease in toxicity to mice, 170-fold decrease in the binding affinity for insect sodium channels. 2
Mutagenesisi27K → D: 118-fold decrease in toxicity to mice, and 250-fold decrease in the binding affinity for insect sodium channels. 2 Publications1
Mutagenesisi28P → S: 1.8-fold decrease in toxicity to mice, and 1.3-fold decrease in the binding affinity for insect sodium channels. 2 Publications1
Mutagenesisi29H → E: 1.7-fold decrease in toxicity to mice, and 0.8-fold decrease in the binding affinity for insect sodium channels. 1 Publication1
Mutagenesisi30N → A: Complete loss of toxicity to mice, 380-fold decrease in the binding affinity for insect sodium channels. 1 Publication1
Mutagenesisi40Y → G: 1.9-fold decrease in toxicity to mice, and 1.4-fold decrease in the binding affinity for insect sodium channels. 1 Publication1
Mutagenesisi47K → E: 4.1-fold decrease in toxicity to mice, and 14-fold decrease in the binding affinity for insect sodium channels. 1 Publication1
Mutagenesisi57W → G: More than 50-fold decrease in toxicity to mice. 1 Publication1
Mutagenesisi61Y → G: More than 50-fold decrease in toxicity to mice. 1 Publication1
Mutagenesisi72D → A: 0.6-fold decrease in toxicity to mice, and 0.2-fold decrease in the binding affinity for insect sodium channels. 1 Publication1
Mutagenesisi77R → A: Complete loss of toxicity to mice, and complete loss of affinity for insect sodium channels. 1 Publication1
Mutagenesisi81K → E: 71.4-fold decrease in toxicity to mice, and 170-fold decrease in the binding affinity for insect sodium channels. 1 Publication1
Mutagenesisi83H → A: 3.8-fold decrease in toxicity to mice, and 180-fold decrease in the binding affinity for insect sodium channels. 1 Publication1
Mutagenesisi83H → D: 43.9-fold decrease in toxicity to mice, and 96-fold decrease in the binding affinity for insect sodium channels. 1 Publication1
Mutagenesisi83H → K: 1.9-fold decrease in toxicity to mice, and 11.5-fold decrease in the binding affinity for insect sodium channels. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 191 PublicationAdd BLAST19
ChainiPRO_000003523620 – 83Alpha-like toxin BmK-M1Add BLAST64
PropeptideiPRO_000003523784Removed by a carboxypeptidaseCurated1

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi31 ↔ 82
Disulfide bondi35 ↔ 55
Disulfide bondi41 ↔ 65
Disulfide bondi45 ↔ 67

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.

Structurei

Secondary structure

184
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi21 – 27Combined sources7
Turni28 – 30Combined sources3
Helixi38 – 47Combined sources10
Beta strandi51 – 59Combined sources9
Beta strandi62 – 70Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DJTX-ray1.20A/B20-83[»]
1SN1X-ray1.70A20-83[»]
1T7AX-ray1.50A20-83[»]
1T7BX-ray1.85A20-83[»]
1T7EX-ray1.40A20-83[»]
1ZU3X-ray1.33A20-83[»]
1ZUTX-ray1.70A20-83[»]
1ZVEX-ray1.70A20-83[»]
1ZVGX-ray1.20A20-83[»]
1ZYVX-ray1.50A20-83[»]
1ZYWX-ray1.30A20-83[»]
ProteinModelPortaliP45697.
SMRiP45697.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP45697.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.30.30.10. 1 hit.
InterProiIPR003614. Scorpion_toxin-like.
IPR018218. Scorpion_toxinL.
IPR002061. Scorpion_toxinL/defensin.
[Graphical view]
PfamiPF00537. Toxin_3. 1 hit.
[Graphical view]
PRINTSiPR00285. SCORPNTOXIN.
SMARTiSM00505. Knot1. 1 hit.
[Graphical view]
SUPFAMiSSF57095. SSF57095. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P45697-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNYLVMISFA LLLMTGVESV RDAYIAKPHN CVYECARNEY CNDLCTKNGA
60 70 80
KSGYCQWVGK YGNGCWCIEL PDNVPIRVPG KCHR
Length:84
Mass (Da):9,496
Last modified:December 15, 1998 - v2
Checksum:iF8F14623AB2549A2
GO

Sequence cautioni

The sequence AAA69557 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti48N → D AA sequence (PubMed:8896191).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF057554 Genomic DNA. Translation: AAC13693.1.
U28659 mRNA. Translation: AAA69557.1. Different initiation.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF057554 Genomic DNA. Translation: AAC13693.1.
U28659 mRNA. Translation: AAA69557.1. Different initiation.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DJTX-ray1.20A/B20-83[»]
1SN1X-ray1.70A20-83[»]
1T7AX-ray1.50A20-83[»]
1T7BX-ray1.85A20-83[»]
1T7EX-ray1.40A20-83[»]
1ZU3X-ray1.33A20-83[»]
1ZUTX-ray1.70A20-83[»]
1ZVEX-ray1.70A20-83[»]
1ZVGX-ray1.20A20-83[»]
1ZYVX-ray1.50A20-83[»]
1ZYWX-ray1.30A20-83[»]
ProteinModelPortaliP45697.
SMRiP45697.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

TCDBi8.B.1.1.1. the long (4c-c) scorpion toxin (l-st) superfamily.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP45697.

Family and domain databases

Gene3Di3.30.30.10. 1 hit.
InterProiIPR003614. Scorpion_toxin-like.
IPR018218. Scorpion_toxinL.
IPR002061. Scorpion_toxinL/defensin.
[Graphical view]
PfamiPF00537. Toxin_3. 1 hit.
[Graphical view]
PRINTSiPR00285. SCORPNTOXIN.
SMARTiSM00505. Knot1. 1 hit.
[Graphical view]
SUPFAMiSSF57095. SSF57095. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSCX1_MESMA
AccessioniPrimary (citable) accession number: P45697
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: December 15, 1998
Last modified: November 2, 2016
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Miscellaneous

Exists in two forms, due to cis-trans isomerization at 28-Pro-His-29.
Has no effect on SCN2A (Nav1.2).1 Publication

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.