Reviewed, UniProtKB/Swiss-Prot P45687 (XYLA_THENE)

Names and origin

Protein names

Recommended name:
    Xylose isomerase
    EC=5.3.1.5

Gene names

Name:

xylA

Organism

Thermotoga neapolitana

Taxonomic identifier

2337 [NCBI]

Taxonomic lineage

Bacteria › Thermotogae › Thermotogales › Thermotogaceae › Thermotoga

Protein attributes

Sequence length	444 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	D-xylose = D-xylulose. HAMAP MF_00455
Cofactor	Binds 2 cobalt ions per subunit. HAMAP MF_00455
Subunit structure	Homotetramer. HAMAP MF_00455
Subcellular location	Cytoplasm. HAMAP MF_00455
Sequence similarities	Belongs to the xylose isomerase family.
biophysicochemical properties	pH dependence: Optimum pH is 7.1. HAMAP MF_00455 Temperature dependence: Optimum temperature is above 95 degrees Celsius. Extremely thermostable.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Pentose shunt Xylose metabolism
Cellular component	Cytoplasm
Ligand	Cobalt Metal-binding
Molecular function	Isomerase
Technical term	3D-structure
Gene Ontology (GO)
Biological process	D-xylose metabolic process Inferred from electronic annotation. Source: HAMAP pentose-phosphate shunt Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cobalt ion binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: HAMAP xylose isomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 444

444

Xylose isomerase HAMAP MF_00455

PRO_0000195814

Sites

Active site

101

1

HAMAP MF_00455

Active site

104

1

By similarity

Metal binding

232

1

Cobalt 1 HAMAP MF_00455

Metal binding

268

1

Cobalt 1 HAMAP MF_00455

Metal binding

268

1

Cobalt 2 HAMAP MF_00455

Metal binding

271

1

Cobalt 2 HAMAP MF_00455

Metal binding

296

1

Cobalt 1 HAMAP MF_00455

Metal binding

307

1

Cobalt 2 HAMAP MF_00455

Metal binding

309

1

Cobalt 2 HAMAP MF_00455

Metal binding

339

1

Cobalt 1 HAMAP MF_00455

Secondary structure

1

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444

Helix Strand Turn

Details...

Beta strand

23 – 26

4

Helix

38 – 42

5

Beta strand

45 – 47

3

Helix

48 – 51

4

Helix

68 – 70

3

Helix

75 – 93

19

Beta strand

97 – 101

5

Helix

102 – 105

4

Helix

112 – 132

21

Beta strand

136 – 141

6

Beta strand

145 – 147

3

Helix

148 – 150

3

Helix

160 – 179

20

Beta strand

183 – 187

5

Beta strand

192 – 195

4

Helix

202 – 222

21

Beta strand

227 – 231

5

Beta strand

237 – 244

8

Helix

247 – 256

10

Helix

260 – 262

3

Beta strand

263 – 268

6

Helix

269 – 274

6

Helix

279 – 288

10

Beta strand

292 – 296

5

Beta strand

304 – 306

3

Helix

315 – 328

14

Beta strand

336 – 338

3

Helix

349 – 376

28

Helix

378 – 386

9

Helix

388 – 390

3

Helix

393 – 400

8

Helix

405 – 412

8

Helix

425 – 441

17

Sequences

	Sequence		Length	Mass (Da)
	P45687-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 21F606C4D5F68D0B	FASTA	444	50,893
	10 20 30 40 50 60 MAEFFPEIPK VQFEGKESTN PLAFKFYDPE EIIDGKPLKD HLKFSVAFWH TFVNEGRDPF 70 80 90 100 110 120 GDPTADRPWN RYTDPMDKAF ARVDALFEFC EKLNIEYFCF HDRDIAPEGK TLRETNKILD 130 140 150 160 170 180 KVVERIKERM KDSNVKLLWG TANLFSHPRY MHGAATTCSA DVFAYAAAQV KKALEITKEL 190 200 210 220 230 240 GGEGYVFWGG REGYETLLNT DLGFELENLA RFLRMAVDYA KRIGFTGQFL IEPKPKEPTK 250 260 270 280 290 300 HQYDFDVATA YAFLKSHGLD EYFKFNIEAN HATLAGHTFQ HELRMARILG KLGSIDANQG 310 320 330 340 350 360 DLLLGWDTDQ FPTNVYDTTL AMYEVIKAGG FTKGGLNFDA KVRRASYKVE DLFIGHIAGM 370 380 390 400 410 420 DTFALGFKVA YKLVKDGVLD KFIEEKYRSF REGIGRDIVE GKVDFEKLEE YIIDKETIEL 430 440 PSGKQEYLES LINSYIVKTI LELR

References

[1]	"xylA cloning and sequencing and biochemical characterization of xylose isomerase from Thermotoga neapolitana." Vieille C., Hess J.M.J., Kelly R.M., Zeikus J.G.J. Appl. Environ. Microbiol. 61:1867-1875(1995) [PubMed: 7646024] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DSM 5068.
[2]	Gallay O., Chopra R., Conti E., Brick P., Jackson R., Hartley B., Vieille C., Zeikus J.G., Blow D. Submitted (NOV-1997) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS). Strain: DSM 5068.

Cross-references

Sequence databases

EMBLGenBankDDBJ

L38994 Genomic DNA. Translation: AAB06798.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1A0E	X-ray	2.70	A/D	2-444	[»]

ModBase

Search...

Enzyme and pathway databases

BRENDA

5.3.1.5. 1590.

Family and domain databases

HAMAP

MF_00455.
[Tree]

InterPro

IPR013022. Xyl_isomerase-like_TIM-brl.
IPR012307. Xyl_isomerase-typ_TIM-brl.
IPR013452. Xylose_isom_bac.
IPR001998. Xylose_isomerase.
IPR018115. Xylose_isomerase_AS.
[Graphical view]

Gene3D

G3DSA:3.20.20.150. Xyl_isomerase-like_TIM-brl. 1 hit.

Pfam

PF01261. AP_endonuc_2. 1 hit.
[Graphical view]

PRINTS

PR00688. XYLOSISMRASE.

TIGRFAMs

TIGR02630. xylose_isom_A. 1 hit.

PROSITE

PS51415. XYLOSE_ISOMERASE. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

XYLA_THENE

Accession

Primary (citable) accession number: P45687

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	June 16, 2009
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)