P45686 (RBS_HALNC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribulose bisphosphate carboxylase small chain
Short name=RuBisCO small subunit
EC=4.1.1.39

Gene names

Name:	cbbS
Synonyms:	rbcS
Ordered Locus Names:	Hneap_0921

Organism

Halothiobacillus neapolitanus (strain ATCC 23641 / c2) (Thiobacillus neapolitanus) [Complete proteome] [HAMAP]

Taxonomic identifier

555778 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Chromatiales › Halothiobacillaceae › Halothiobacillus › Thiobacillus neapolitanus

Protein attributes

Sequence length	110 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate. Both reactions occur simultaneously and in competition at the same active site.
Catalytic activity	2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O. 3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.
Subunit structure	8 large chains + 8 small chains.
Sequence similarities	Belongs to the RuBisCO small chain family.

Ontologies

Keywords
Biological process	Calvin cycle Carbon dioxide fixation
Molecular function	Lyase Monooxygenase Oxidoreductase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological_process	reductive pentose-phosphate cycle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	monooxygenase activity Inferred from electronic annotation. Source: UniProtKB-KW ribulose-bisphosphate carboxylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 110

110

Ribulose bisphosphate carboxylase small chain

PRO_0000198631

Secondary structure

1

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110

Helix Strand Turn

Details...

Turn

15 – 18

4

Helix

24 – 36

13

Beta strand

40 – 46

7

Helix

48 – 50

3

Beta strand

57 – 60

4

Helix

69 – 82

14

Beta strand

86 – 94

9

Turn

95 – 98

4

Beta strand

99 – 107

9

Sequences

	Sequence		Length	Mass (Da)
	P45686 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 9C4F1AB8D29B104D	FASTA	110	12,855
	10 20 30 40 50 60 MAEMQDYKQS LKYETFSYLP PMNAERIRAQ IKYAIAQGWS PGIEHVEVKN SMNQYWYMWK 70 80 90 100 110 LPFFGEQNVD NVLAEIEACR SAYPTHQVKL VAYDNYAQSL GLAFVVYRGN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Insertion mutation of the form I cbbL gene encoding ribulose bisphosphate carboxylase/oxygenase (RuBisCO) in Thiobacillus neapolitanus results in expression of form II RuBisCO, loss of carboxysomes, and an increased CO2 requirement for growth." Baker S.H., Jin S., Aldrich H.C., Howard G.T., Shively J.M. J. Bacteriol. 180:4133-4139(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete sequence of Halothiobacillus neapolitanus c2." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D., Goodwin L., Pitluck S., Davenport K., Brettin T., Detter J.C., Han C., Tapia R., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N. , Kerfeld C., Cannon G., Heinhort S. Submitted (OCT-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 23641 / c2.
[3]	"The structure of Halothiobacillus neapolitanus Rubisco." Kerfeld C.A., Sawaya M.R., Pashkov I., Cannon G., Williams E., Tran K., Yeates T.O. Submitted (APR-2005) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF038430 Genomic DNA. Translation: AAC32550.1.
CP001801 Genomic DNA. Translation: ACX95764.1.

RefSeq

YP_003262811.1. NC_013422.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1SVD	X-ray	1.80	M	1-110	[»]

ProteinModelPortal

P45686.

SMR

P45686. Positions 3-110.

ModBase

Search...

Protein-protein interaction databases

STRING

555778.Hneap_0921.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

ACX95764; ACX95764; Hneap_0921.

GeneID

8534062.

KEGG

hna:Hneap_0921.

PATRIC

32205716. VBIHalNea120669_0940.

Organism-specific databases

CMR

Search...

Phylogenomic databases

eggNOG

COG4451.

HOGENOM

HOG000141332.

KO

K01602.

OMA

WNPAIEH.

Enzyme and pathway databases

BioCyc

HNEA555778:GIVV-950-MONOMER.

Family and domain databases

Gene3D

3.30.190.10. 1 hit.

InterPro

IPR000894. RuBisCO_sc_dom.
[Graphical view]

Pfam

PF00101. RuBisCO_small. 1 hit.
[Graphical view]

SMART

SM00961. RuBisCO_small. 1 hit.
[Graphical view]

SUPFAM

SSF55239. RuBisCO_small. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

P45686.

Entry information

Entry name

RBS_HALNC

Accession

Primary (citable) accession number: P45686
Secondary accession number(s): D0KZ91

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program