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Protein

RNA polymerase sigma factor RpoS

Gene

rpoS

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Protein inferred from homologyi

Functioni

Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is the master transcriptional regulator of the stationary phase and the general stress response.UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi293 – 312H-T-H motifUniRule annotationAdd BLAST20

GO - Molecular functioni

GO - Biological processi

  • negative regulation of secondary metabolite biosynthetic process Source: PseudoCAP
  • positive regulation of chemotaxis Source: PseudoCAP
  • positive regulation of single-species biofilm formation on inanimate substrate Source: PseudoCAP
  • regulation of cell motility Source: PseudoCAP
  • regulation of cellular response to heat Source: PseudoCAP
  • regulation of cellular response to oxidative stress Source: PseudoCAP
  • regulation of response to salt stress Source: PseudoCAP
  • regulation of transcription, DNA-templated Source: PseudoCAP
  • response to stress Source: UniProtKB-HAMAP
  • transcription initiation from bacterial-type RNA polymerase promoter Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Sigma factor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
RNA polymerase sigma factor RpoSUniRule annotation
Alternative name(s):
Sigma SUniRule annotation
Sigma-38UniRule annotation
Gene namesi
Name:rpoSUniRule annotation
Ordered Locus Names:PA3622
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA3622.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000939751 – 334RNA polymerase sigma factor RpoSAdd BLAST334

Proteomic databases

PaxDbiP45684.

Interactioni

Subunit structurei

Interacts with the RNA polymerase core enzyme.UniRule annotation

Protein-protein interaction databases

STRINGi208964.PA3622.

Structurei

3D structure databases

ProteinModelPortaliP45684.
SMRiP45684.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni61 – 94Sigma-70 factor domain-1UniRule annotationAdd BLAST34
Regioni99 – 169Sigma-70 factor domain-2UniRule annotationAdd BLAST71
Regioni179 – 254Sigma-70 factor domain-3UniRule annotationAdd BLAST76
Regioni267 – 320Sigma-70 factor domain-4UniRule annotationAdd BLAST54

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi123 – 126Interaction with polymerase core subunit RpoC4

Sequence similaritiesi

Belongs to the sigma-70 factor family. RpoS subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DG1. Bacteria.
COG0568. LUCA.
HOGENOMiHOG000270273.
InParanoidiP45684.
KOiK03087.
OMAiKYIDYTR.
PhylomeDBiP45684.

Family and domain databases

Gene3Di1.10.10.10. 2 hits.
HAMAPiMF_00959. Sigma70_RpoS. 1 hit.
InterProiIPR014284. RNA_pol_sigma-70_dom.
IPR000943. RNA_pol_sigma70.
IPR009042. RNA_pol_sigma70_r1_2.
IPR007627. RNA_pol_sigma70_r2.
IPR007624. RNA_pol_sigma70_r3.
IPR007630. RNA_pol_sigma70_r4.
IPR013325. RNA_pol_sigma_r2.
IPR013324. RNA_pol_sigma_r3_r4.
IPR012761. RNA_pol_sigma_RpoS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00140. Sigma70_r1_2. 1 hit.
PF04542. Sigma70_r2. 1 hit.
PF04539. Sigma70_r3. 1 hit.
PF04545. Sigma70_r4. 1 hit.
[Graphical view]
PRINTSiPR00046. SIGMA70FCT.
SUPFAMiSSF88659. SSF88659. 2 hits.
SSF88946. SSF88946. 1 hit.
TIGRFAMsiTIGR02394. rpoS_proteo. 1 hit.
TIGR02937. sigma70-ECF. 1 hit.
PROSITEiPS00715. SIGMA70_1. 1 hit.
PS00716. SIGMA70_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P45684-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALKKEGPEF DHDDEVLLLE PGIMLDESSA DEQPSPRATP KATTSFSSKQ
60 70 80 90 100
HKHIDYTRAL DATQLYLNEI GFSPLLTPEE EVHFARLAQK GDPAGRKRMI
110 120 130 140 150
ESNLRLVVKI ARRYVNRGLS LLDLIEEGNL GLIRAVEKFD PERGFRFSTY
160 170 180 190 200
ATWWIRQTIE RAIMNQTRTI RLPIHVVKEL NVYLRAAREL THKLDHEPSP
210 220 230 240 250
EEIANLLEKP VAEVKRMLGL NERVTSVDVS LGPDSDKTLL DTLTDDRPTD
260 270 280 290 300
PCELLQDDDL SESIDQWLTE LTDKQREVVI RRFGLRGHES STLEEVGQEI
310 320 330
GLTRERVRQI QVEALKRLRE ILEKNGLSSD ALFQ
Length:334
Mass (Da):38,235
Last modified:November 1, 1995 - v1
Checksum:iB049427E5CBDC9DD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D26134 Genomic DNA. Translation: BAA05131.1.
AE004091 Genomic DNA. Translation: AAG07010.1.
PIRiS55064.
RefSeqiNP_252312.1. NC_002516.2.
WP_003113871.1. NZ_ASJY01000588.1.

Genome annotation databases

EnsemblBacteriaiAAG07010; AAG07010; PA3622.
GeneIDi880421.
KEGGipae:PA3622.
PATRICi19841867. VBIPseAer58763_3791.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D26134 Genomic DNA. Translation: BAA05131.1.
AE004091 Genomic DNA. Translation: AAG07010.1.
PIRiS55064.
RefSeqiNP_252312.1. NC_002516.2.
WP_003113871.1. NZ_ASJY01000588.1.

3D structure databases

ProteinModelPortaliP45684.
SMRiP45684.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA3622.

Proteomic databases

PaxDbiP45684.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG07010; AAG07010; PA3622.
GeneIDi880421.
KEGGipae:PA3622.
PATRICi19841867. VBIPseAer58763_3791.

Organism-specific databases

PseudoCAPiPA3622.

Phylogenomic databases

eggNOGiENOG4105DG1. Bacteria.
COG0568. LUCA.
HOGENOMiHOG000270273.
InParanoidiP45684.
KOiK03087.
OMAiKYIDYTR.
PhylomeDBiP45684.

Family and domain databases

Gene3Di1.10.10.10. 2 hits.
HAMAPiMF_00959. Sigma70_RpoS. 1 hit.
InterProiIPR014284. RNA_pol_sigma-70_dom.
IPR000943. RNA_pol_sigma70.
IPR009042. RNA_pol_sigma70_r1_2.
IPR007627. RNA_pol_sigma70_r2.
IPR007624. RNA_pol_sigma70_r3.
IPR007630. RNA_pol_sigma70_r4.
IPR013325. RNA_pol_sigma_r2.
IPR013324. RNA_pol_sigma_r3_r4.
IPR012761. RNA_pol_sigma_RpoS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00140. Sigma70_r1_2. 1 hit.
PF04542. Sigma70_r2. 1 hit.
PF04539. Sigma70_r3. 1 hit.
PF04545. Sigma70_r4. 1 hit.
[Graphical view]
PRINTSiPR00046. SIGMA70FCT.
SUPFAMiSSF88659. SSF88659. 2 hits.
SSF88946. SSF88946. 1 hit.
TIGRFAMsiTIGR02394. rpoS_proteo. 1 hit.
TIGR02937. sigma70-ECF. 1 hit.
PROSITEiPS00715. SIGMA70_1. 1 hit.
PS00716. SIGMA70_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRPOS_PSEAE
AccessioniPrimary (citable) accession number: P45684
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 2, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.