P45648 (RNPA_COXBU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease P protein component
Short name=RNase P protein
Short name=RNaseP protein
EC=3.1.26.5

Alternative name(s):
Protein C5

Gene names

Name:	rnpA
Ordered Locus Names:	CBU_1918

Organism

Coxiella burnetii (strain RSA 493 / Nine Mile phase I) [Reference proteome] [HAMAP]

Taxonomic identifier

227377 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Coxiellaceae › Coxiella › Coxiella burnetii

Protein attributes

Sequence length	121 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	RNaseP catalyzes the removal of the 5'-leader sequence from pre-tRNA to produce the mature 5'-terminus. It can also cleave other RNA substrates such as 4.5S RNA. The protein component plays an auxiliary but essential role in vivo by binding to the 5'-leader sequence and broadening the substrate specificity of the ribozyme By similarity. HAMAP-Rule MF_00227
Catalytic activity	Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor. HAMAP-Rule MF_00227
Subunit structure	Consists of a catalytic RNA component (M1 or RnpB) and a protein subunit By similarity.
Sequence similarities	Belongs to the RnpA family.
Sequence caution	The sequence AAO91409.2 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	tRNA processing
Ligand	RNA-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC tRNA 5'-leader removal Inferred from electronic annotation. Source: HAMAP
Molecular_function	ribonuclease P activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 121	121	Ribonuclease P protein component HAMAP-Rule MF_00227		PRO_0000198455

Sequences

	Sequence		Length	Mass (Da)
	P45648 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: E9402C53D6206EFE	FASTA	121	14,398
	10 20 30 40 50 60 MEKGFSVGWR IRTTAEFRRI YAARQRIIGR YYLLYYRENE IKHSRLGVVA SKRNVRKAVW 70 80 90 100 110 120 RNRVRRVVKE AFRIRKKDLP AFDIVVVAKA SSVEADNKEL YECINKLFTQ LEKQSKRSSS V

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of an autonomous replication sequence from Coxiella burnetii." Suhan M., Chen S.Y., Thompson H.A., Hoover T.A., Hill A., Williams J.C. J. Bacteriol. 176:5233-5243(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Nine Mile phase I / Bratislava.
[2]	"Complete genome sequence of the Q-fever pathogen, Coxiella burnetii." Seshadri R., Paulsen I.T., Eisen J.A., Read T.D., Nelson K.E., Nelson W.C., Ward N.L., Tettelin H., Davidsen T.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Brinkac L.M., Madupu R., Dodson R.J., Khouri H.M., Lee K.H., Carty H.A. , Scanlan D., Heinzen R.A., Thompson H.A., Samuel J.E., Fraser C.M., Heidelberg J.F. Proc. Natl. Acad. Sci. U.S.A. 100:5455-5460(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RSA 493 / Nine Mile phase I.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U10529 Genomic DNA. Translation: AAA56917.1. AE016828 Genomic DNA. Translation: AAO91409.2. Different initiation.
PIR	I40652.
RefSeq	NP_820895.2. NC_002971.3.
3D structure databases
ProteinModelPortal	P45648.
ModBase	Search...
Protein-protein interaction databases
STRING	227377.CBU_1918.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAO91409; AAO91409; CBU_1918.
GeneID	1209831.
KEGG	cbu:CBU_1918.
PATRIC	17932571. VBICoxBur82552_1902.
Phylogenomic databases
eggNOG	COG0594.
HOGENOM	HOG000266301.
KO	K03536.
OMA	FTQLERQ.
ProtClustDB	CLSK915125.
Enzyme and pathway databases
BioCyc	CBUR227377:GJ7S-1892-MONOMER.
Family and domain databases
Gene3D	3.30.230.10. 1 hit.
HAMAP	MF_00227. RNase_P.
InterPro	IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. IPR000100. RNase_P. IPR020539. RNase_P_CS. [Graphical view]
Pfam	PF00825. Ribonuclease_P. 1 hit. [Graphical view]
ProDom	PD003629. Ribonuclease_P. 1 hit. [Graphical view] [Entries sharing at least one domain]
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00188. rnpA. 1 hit.
PROSITE	PS00648. RIBONUCLEASE_P. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNPA_COXBU

Accession

Primary (citable) accession number: P45648

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program