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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Azospirillum brasilense
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei243ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
OrganismiAzospirillum brasilense
Taxonomic identifieri192 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodospirillalesRhodospirillaceaeAzospirillum

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001194961 – 446Glutamate--tRNA ligaseAdd BLAST446

Proteomic databases

PRIDEiP45631

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliP45631
SMRiP45631
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi9 – 19"HIGH" regionUniRule annotationAdd BLAST11
Motifi240 – 244"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.UniRule annotation

Family and domain databases

CDDicd00808 GluRS_core, 1 hit
Gene3Di1.10.10.350, 1 hit
3.40.50.620, 1 hit
HAMAPiMF_00022 Glu_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR008925 aa-tRNA-synth_I_codon-bd
IPR020751 aa-tRNA-synth_I_codon-bd_sub2
IPR001412 aa-tRNA-synth_I_CS
IPR004527 Glu-tRNA-ligase_bac/mito
IPR000924 Glu/Gln-tRNA-synth
IPR020058 Glu/Gln-tRNA-synth_Ib_cat-dom
IPR033910 GluRS_core
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF00749 tRNA-synt_1c, 1 hit
PRINTSiPR00987 TRNASYNTHGLU
SUPFAMiSSF48163 SSF48163, 1 hit
TIGRFAMsiTIGR00464 gltX_bact, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

P45631-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVAVPFAPS PTGLLHVGNV RLALVNWLFA RKAGGNFLVR LDDTDEERSK
60 70 80 90 100
PEYAEGIERD LTWLGLTWDR FARESDRYGA TDEVAAALKA SGRLYPCYET
110 120 130 140 150
PEELNLKRAS LSSQGRPPIY DRAALRLGDA DRARLEAEGR KPHWRFKLEH
160 170 180 190 200
TPVEWTDLVR GPVHFEGSAL SDPVLIAEDG RPLYTLTSVV DDADLAITHV
210 220 230 240 250
IRGEDHLANT AVQIQIFEAV GGAVPVFAHL PLLTDATGQG LSKRLGSLSV
260 270 280 290 300
ASLREEEGIE PMALASLLAK LGTSDAIEPR LTLDELVAEF DIAKVSRATP
310 320 330 340 350
KFDPEELLRL NARILHLLPF ERVAGELAAS VWMMPTPAFW EAVPNLSRVA
360 370 380 390 400
EARDWWAVTH APVARRRTIP LFLAEAATLL PKEPWDLSTW GTWTGAVKAK
410 420 430 440
TGRKGKDLFL PLRRALTGRD HGGQLKNLLP LIGRTRAHKR LAGETA
Length:446
Mass (Da):49,179
Last modified:December 15, 1998 - v2
Checksum:iA7CE141B79416572
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti6P → R in AAA61907 (Ref. 1) Curated1
Sequence conflicti39V → L in AAA61907 (Ref. 1) Curated1
Sequence conflicti80 – 81AT → RY in AAA61907 (Ref. 1) Curated2
Sequence conflicti112S → V in AAA61907 (Ref. 1) Curated1
Sequence conflicti137A → S in AAA61907 (Ref. 1) Curated1
Sequence conflicti142P → R in AAA61907 (Ref. 1) Curated1
Sequence conflicti168S → A in AAA61907 (Ref. 1) Curated1
Sequence conflicti177A → R in AAA61907 (Ref. 1) Curated1
Sequence conflicti207L → V in AAA61907 (Ref. 1) Curated1
Sequence conflicti220 – 223VGGA → LTNSEGGGV (Ref. 1) Curated4
Sequence conflicti378T → A in AAA61907 (Ref. 1) Curated1
Sequence conflicti382K → E in AAA61907 (Ref. 1) Curated1
Sequence conflicti423 – 424GQ → PE in AAA61907 (Ref. 1) Curated2
Sequence conflicti438H → E in AAA61907 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U17699 Genomic DNA Translation: AAA61907.1
X99587 Genomic DNA Translation: CAA67900.1

Similar proteinsi

Entry informationi

Entry nameiSYE_AZOBR
AccessioniPrimary (citable) accession number: P45631
Secondary accession number(s): O07044
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: December 15, 1998
Last modified: May 23, 2018
This is version 95 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

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