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Protein

Glutamine synthetase

Gene

glnA

Organism
Lactobacillus delbrueckii subsp. bulgaricus
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Glutamine synthetase (GS) is an unusual multitasking protein that functions as an enzyme, a transcription coregulator, and a chaperone in ammonium assimilation and in the regulation of genes involved in nitrogen metabolism. It catalyzes the ATP-dependent biosynthesis of glutamine from glutamate and ammonia. Feedback-inhibited GlnA also interacts with and regulates the activity of the transcriptional regulator TnrA. During nitrogen limitation, TnrA is in its DNA-binding active state and turns on the transcription of genes required for nitrogen assimilation. Under conditions of nitrogen excess, feedback-inhibited GlnA forms a stable complex with TnrA, which inhibits its DNA-binding activity. In contrast, feedback-inhibited GlnA acts as a chaperone to stabilize the DNA-binding activity of GlnR, which represses the transcription of nitrogen assimilation genes.By similarity

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.By similarity

Cofactori

Mg2+By similarityNote: Binds 2 Mg2+ ions per subunit.By similarity

Enzyme regulationi

Inhibited by glutamine.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei63Important for inhibition by glutamineBy similarity1
Metal bindingi134Magnesium 1By similarity1
Metal bindingi136Magnesium 2By similarity1
Binding sitei186ATPBy similarity1
Metal bindingi191Magnesium 2By similarity1
Metal bindingi198Magnesium 2By similarity1
Binding sitei243L-glutamate; via carbonyl oxygenBy similarity1
Metal bindingi247Magnesium 1; via pros nitrogenBy similarity1
Binding sitei251ATPBy similarity1
Binding sitei300L-glutamateBy similarity1
Binding sitei306L-glutamateBy similarity1
Binding sitei318ATPBy similarity1
Binding sitei318L-glutamateBy similarity1
Binding sitei323ATPBy similarity1
Metal bindingi335Magnesium 1By similarity1
Binding sitei337L-glutamateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetase1 Publication (EC:6.3.1.2By similarity)
Short name:
GS1 Publication
Alternative name(s):
Glutamate--ammonia ligaseBy similarity
Glutamine synthetase I alphaBy similarity
Short name:
GSI alphaBy similarity
Gene namesi
Name:glnA1 Publication
OrganismiLactobacillus delbrueckii subsp. bulgaricus
Taxonomic identifieri1585 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001532411 – 445Glutamine synthetaseAdd BLAST445

Interactioni

Subunit structurei

Oligomer of 12 subunits arranged in the form of two hexagons. In its feedback-inhibited form, interacts with TnrA in order to block its DNA-binding activity.By similarity

Structurei

3D structure databases

ProteinModelPortaliP45627.
SMRiP45627.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamine synthetase family.Curated

Phylogenomic databases

eggNOGiENOG4105C5F. Bacteria.
COG0174. LUCA.

Family and domain databases

Gene3Di3.10.20.70. 1 hit.
3.30.590.10. 1 hit.
InterProiView protein in InterPro
IPR008147. Gln_synt_b-grasp.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR004809. Gln_synth_I.
IPR027302. Gln_synth_N_conserv_site.
PfamiView protein in Pfam
PF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
SMARTiView protein in SMART
SM01230. Gln-synt_C. 1 hit.
SUPFAMiSSF54368. SSF54368. 1 hit.
TIGRFAMsiTIGR00653. GlnA. 1 hit.
PROSITEiView protein in PROSITE
PS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.

Sequencei

Sequence statusi: Complete.

P45627-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKVITEEEI RKDVEEKNVR FLRLAFTDIN GTLKNLEVPV SQLDDVLGNQ
60 70 80 90 100
TRFDGSSIDG FVRLEESDMV LYPDLATWLV LAWTTVEEGT IGRLVCSVHN
110 120 130 140 150
VDGTPFEGDP RNNLKKVIAE MEEMGFSDFE IGFEAEFFLF KEGKNGEETT
160 170 180 190 200
KVSDHSSYFD MASEDEGAKC RREIVETLEK LGFRVEAAHH EVGDGQQEID
210 220 230 240 250
FRFDNALATA DKLQTFKMVV KTIARKYHLH ASFMAKPVEG LAGNGMHTNM
260 270 280 290 300
SLLKDGKNAF YDKDGQYNLS TTALTFLNGI LEHARAITCV ANPTVNSYKR
310 320 330 340 350
LIPGFEAPVY ISWASRNRSP MVRIPNANEV GTRLEMRSTD PTANPYLLLS
360 370 380 390 400
ACLKAGLTGI KEGKLPMAPV TSNLFEMTDD ERKELGIKPL PSTLHNAIKA
410 420 430 440
FKEDEVVKSA FSEHIVDSFL ELKETEWALY TQSVSEWEVK RYFNY
Length:445
Mass (Da):50,134
Last modified:November 1, 1995 - v1
Checksum:iA57A9E11ABAF87E8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10020 Genomic DNA. Translation: BAA00910.1.
PIRiA48947.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiGLN1A_LACDE
AccessioniPrimary (citable) accession number: P45627
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: July 5, 2017
This is version 73 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families