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P45626 (GLNA2_RHIML) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamine synthetase 2

EC=6.3.1.2
Alternative name(s):
Glutamate--ammonia ligase II
Glutamine synthetase II
Short name=GSII
Gene names
Name:glnII
Encoded onPlasmid pSymB (megaplasmid 2)
OrganismRhizobium meliloti (Ensifer meliloti) (Sinorhizobium meliloti)
Taxonomic identifier382 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium

Protein attributes

Sequence length329 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

Subunit structure

Homooctamer.

Subcellular location

Cytoplasm.

Miscellaneous

Two forms of glutamine synthetase (GSI and GSII) can be found in this nitrogen fixing bacteria, GSI is a typical prokaryotic glutamine synthetase whereas GSII is similar to the eukaryotic enzyme.

Sequence similarities

Belongs to the glutamine synthetase family.

Ontologies

Keywords
   Biological processNitrogen fixation
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termPlasmid
Gene Ontology (GO)
   Biological_processglutamine biosynthetic process

Inferred from electronic annotation. Source: InterPro

nitrogen fixation

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

glutamate-ammonia ligase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 329329Glutamine synthetase 2
PRO_0000153226

Sequences

Sequence LengthMass (Da)Tools
P45626 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: AF74BB020F7D637D

FASTA32936,966
        10         20         30         40         50         60 
MTKYKLEYIW LDATRPYQTL RGKTQIKEFD AFPTLEQLPL WGFDGSSTLQ AEGRTSDCVL 

        70         80         90        100        110        120 
KPVTVYPDPV RTNGALVMCE VMMPDAETPH ASNTRATVLD DEGAWFGFEQ EYFFYKNGRP 

       130        140        150        160        170        180 
LGFPEQGYPA PQGPYYTGVG YKNVGDVARQ IVEEHLDICL AAGINHEGIN AEVAKGQWEF 

       190        200        210        220        230        240 
QIFGKGSKKA ADEVCVARYL LVRLTEKYGI DVEFHCKPLG DTDWNGSGMH ANFSTAYLRE 

       250        260        270        280        290        300 
VGGQDYFEAL MAAFEKNLHD HINVYGPDNH LRLTGKHETA PWDKFSYGVA DRGASIRVPH 

       310        320 
SFVNNAYPGY LEDRRANSQG DPYQMLLSS 

« Hide

References

[1]"Glutamine synthetase II in Rhizobium: reexamination of the proposed horizontal transfer of DNA from eukaryotes to prokaryotes."
Shatters R.G., Kahn M.L.
J. Mol. Evol. 29:422-428(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X17523 Genomic DNA. Translation: CAB37407.1.

3D structure databases

ProteinModelPortalP45626.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

eggNOGCOG0174.

Family and domain databases

Gene3D3.30.590.10. 1 hit.
InterProIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMSSF54368. SSF54368. 1 hit.
PROSITEPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGLNA2_RHIML
AccessionPrimary (citable) accession number: P45626
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: October 16, 2013
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families