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P45623

- HEM2_SELMA

UniProt

P45623 - HEM2_SELMA

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Protein

Delta-aminolevulinic acid dehydratase, chloroplastic

Gene

HEMB

Organism
Selaginella martensii (Martens's spike moss)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen (By similarity).By similarity

Catalytic activityi

2 5-aminolevulinate = porphobilinogen + 2 H2O.

Cofactori

Mg2+By similarityNote: Binds 2 magnesium ions per monomer. The first magnesium ion is required for catalysis. The second functions as allosteric activator.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei286 – 2861Schiff-base intermediate with substrateBy similarity
Binding sitei296 – 2961Substrate 1By similarity
Binding sitei308 – 3081Substrate 1By similarity
Metal bindingi324 – 3241MagnesiumBy similarity
Active sitei339 – 3391Schiff-base intermediate with substrateBy similarity
Binding sitei365 – 3651Substrate 2By similarity
Binding sitei404 – 4041Substrate 2By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. porphobilinogen synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. chlorophyll biosynthetic process Source: UniProtKB-KW
  2. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Chlorophyll biosynthesis, Heme biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00251; UER00318.

Names & Taxonomyi

Protein namesi
Recommended name:
Delta-aminolevulinic acid dehydratase, chloroplastic (EC:4.2.1.24)
Short name:
ALAD
Short name:
ALADH
Alternative name(s):
Porphobilinogen synthase
Gene namesi
Name:HEMB
Synonyms:ALA2
OrganismiSelaginella martensii (Martens's spike moss)
Taxonomic identifieri3247 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaLycopodiidaeSelaginellalesSelaginellaceaeSelaginella

Subcellular locationi

GO - Cellular componenti

  1. chloroplast Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4040ChloroplastSequence AnalysisAdd
BLAST
Chaini41 – 417377Delta-aminolevulinic acid dehydratase, chloroplasticPRO_0000013319Add
BLAST

Interactioni

Subunit structurei

Homooctamer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP45623.
SMRiP45623. Positions 94-413.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ALAD family.Curated

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR001731. Porphobilinogen_synth.
[Graphical view]
PANTHERiPTHR11458. PTHR11458. 1 hit.
PfamiPF00490. ALAD. 1 hit.
[Graphical view]
PRINTSiPR00144. DALDHYDRTASE.
SMARTiSM01004. ALAD. 1 hit.
[Graphical view]
PROSITEiPS00169. D_ALA_DEHYDRATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P45623-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAALLVPGGG AAPGLVWRRR RAAVQCAAAS PSSPDPSWRT NVVSDRVSVE
60 70 80 90 100
PSPRTIHECE ADVVSGNPPA APAAPAKAKA PPGTPVVKPL RLTSRPRRNR
110 120 130 140 150
KSAALRDAFQ ETTLTPANFI LPLFIHEGEE DSPIGAMPGC SRLGWRHGLI
160 170 180 190 200
DEVYKARDVG VNSVVLFPKI PDALKSSTGD EAYNPDGLVP RAIRTLKDKF
210 220 230 240 250
PDLVIYTDVA LDPYSSDGHD GIVREDGVIM NDETVHQLCK QAVAQAEAGA
260 270 280 290 300
DVVSPSDMMD GRVGAIRTAL DEAGYYHVSI MAYTAKYASA FYEPFREELD
310 320 330 340 350
SNPRFGDKKT YQMNPENYRE ALLEVHADES EGADILMVKP AMPYLHVIRL
360 370 380 390 400
LRDTSALPIS AYQVSGEYSM IKAAASQGML DEKKAILESL LCIKRAGADV
410
ILTYAALQAA RWLCGEK
Length:417
Mass (Da):45,182
Last modified:November 1, 1995 - v1
Checksum:i036E57A607886759
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75043 Genomic DNA. Translation: CAA52955.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75043 Genomic DNA. Translation: CAA52955.1 .

3D structure databases

ProteinModelPortali P45623.
SMRi P45623. Positions 94-413.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00251 ; UER00318 .

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
InterProi IPR013785. Aldolase_TIM.
IPR001731. Porphobilinogen_synth.
[Graphical view ]
PANTHERi PTHR11458. PTHR11458. 1 hit.
Pfami PF00490. ALAD. 1 hit.
[Graphical view ]
PRINTSi PR00144. DALDHYDRTASE.
SMARTi SM01004. ALAD. 1 hit.
[Graphical view ]
PROSITEi PS00169. D_ALA_DEHYDRATASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Sollbach M., Schneider-Poetsch H.A.W.
    Submitted (SEP-1993) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiHEM2_SELMA
AccessioniPrimary (citable) accession number: P45623
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 26, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3