Reviewed, UniProtKB/Swiss-Prot P45623 (HEM2_SELMA)

Names and origin

Protein names

Recommended name:
    Delta-aminolevulinic acid dehydratase, chloroplastic
      Short name=ALADH
      Short name=ALAD
    EC=4.2.1.24
Alternative name(s):
    Porphobilinogen synthase

Gene names

Name:	HEMB
Synonyms:	ALA2

Organism

Selaginella martensii (Martens's spike moss)

Taxonomic identifier

3247 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Lycopodiophyta › Isoetopsida › Selaginellales › Selaginellaceae › Selaginella

Protein attributes

Sequence length	417 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2 5-aminolevulinate = porphobilinogen + 2 H2O.
Cofactor	Magnesium.
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 1/4.
Subunit structure	Homooctamer By similarity.
Subcellular location	Plastid › chloroplast.
Sequence similarities	Belongs to the ALADH family.

Ontologies

Keywords
Biological process	Chlorophyll biosynthesis Porphyrin biosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	Magnesium
Molecular function	Lyase
Gene Ontology (GO)
Biological process	chlorophyll biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW porphobilinogen synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 40	40	Chloroplast Potential
	Chain	41 – 417	377	Delta-aminolevulinic acid dehydratase, chloroplastic		PRO_0000013319
Regions
	Region	207 – 225	19	Magnesium-binding By similarity
Sites
	Active site	339	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P45623-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 036E57A607886759	FASTA	417	45,182
	10 20 30 40 50 60 MAALLVPGGG AAPGLVWRRR RAAVQCAAAS PSSPDPSWRT NVVSDRVSVE PSPRTIHECE 70 80 90 100 110 120 ADVVSGNPPA APAAPAKAKA PPGTPVVKPL RLTSRPRRNR KSAALRDAFQ ETTLTPANFI 130 140 150 160 170 180 LPLFIHEGEE DSPIGAMPGC SRLGWRHGLI DEVYKARDVG VNSVVLFPKI PDALKSSTGD 190 200 210 220 230 240 EAYNPDGLVP RAIRTLKDKF PDLVIYTDVA LDPYSSDGHD GIVREDGVIM NDETVHQLCK 250 260 270 280 290 300 QAVAQAEAGA DVVSPSDMMD GRVGAIRTAL DEAGYYHVSI MAYTAKYASA FYEPFREELD 310 320 330 340 350 360 SNPRFGDKKT YQMNPENYRE ALLEVHADES EGADILMVKP AMPYLHVIRL LRDTSALPIS 370 380 390 400 410 AYQVSGEYSM IKAAASQGML DEKKAILESL LCIKRAGADV ILTYAALQAA RWLCGEK

References

[1]	Sollbach M., Schneider-Poetsch H.A.W. Submitted (SEP-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	X75043 Genomic DNA. Translation: CAA52955.1.
3D structure databases
HSSP	HSSP built from PDB template 1GZG based on UniProtKB Q59643.
ModBase	Search...
Enzyme and pathway databases
BRENDA	4.2.1.24. 228848.
Family and domain databases
InterPro	IPR001731. 4pyrrol_synth_porphobiln_synth. IPR013785. Aldolase_TIM. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR11458. AlaD_dehydratase. 1 hit.
Pfam	PF00490. ALAD. 1 hit. [Graphical view]
PRINTS	PR00144. DALDHYDRTASE.
ProDom	PD002304. AlaD_dehydratase. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00169. D_ALA_DEHYDRATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEM2_SELMA

Accession

Primary (citable) accession number: P45623

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	June 16, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)